In Vitro Cytotoxicity and Setting Time Assessment of Calcium-Enriched Mixture Cement, Retro Mineral Trioxide Aggregate and Mineral Trioxide Aggregate

In Vitro Cytotoxicity and Setting Time Assessment of Calcium-Enriched Mixture Cement, Retro Mineral Trioxide Aggregate and Mineral Trioxide Aggregate

The present study sought to evaluate and compare biocompatibility and setting time of Retro mineral trioxide aggregate (MTA), calcium-enriched mixture (CEM) and Angelus MTA. CEM cement, Angelus MTA and Retro MTA were assessed in set and fresh states. Extracts transformed to each cavity of three 24-w...

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Veröffentlicht in:Iranian endodontic journal 2017-01, Vol.12 (4), p.488-492
Hauptverfasser: Pornamazeh, Tahereh, Yadegari, Zahra, Ghasemi, Amir, Sheykh-Al-Eslamian, Seyedeh Mahsa, Shojaeian, Shiva
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container_end_page 492
container_issue 4
container_start_page 488
container_title Iranian endodontic journal
container_volume 12
creator Pornamazeh, Tahereh
Yadegari, Zahra
Ghasemi, Amir
Sheykh-Al-Eslamian, Seyedeh Mahsa
Shojaeian, Shiva
description The present study sought to evaluate and compare biocompatibility and setting time of Retro mineral trioxide aggregate (MTA), calcium-enriched mixture (CEM) and Angelus MTA. CEM cement, Angelus MTA and Retro MTA were assessed in set and fresh states. Extracts transformed to each cavity of three 24-well plates in which 1×10 cell were seeded into each well 24 h earlier. All specimens were incubated in a humidified incubator with 5% CO2 at 37 C. Mosmann's tetrazolium toxicity (MTT) assay was used to determine cytotoxicity on L929 mouse fibroblast cell line. Cell viability was determined at 1, 24, and 72 h after exposure. The initial setting time was measured by 113.4 g Gilmore needle testing. Then, final setting times were assessed by the 456.5 g Gilmore needle. Data comparisons were performed using the analysis of variance (ANOVA) and Tukey's post hoc test ( =0.05). All groups in both forms indicated higher cell vitality compared to positive control group (
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Retro MTA showed significantly lower initial and final setting time compared to CEM and Angelus MTA ( &lt;0.001). Our results indicated the good cell viability values of Retro MTA and relatively short period of setting time. It seems a promising alternative material in clinical situations where accelerated setting is required. 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title In Vitro Cytotoxicity and Setting Time Assessment of Calcium-Enriched Mixture Cement, Retro Mineral Trioxide Aggregate and Mineral Trioxide Aggregate
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