Comparative analysis of double-stranded RNA degradation and processing in insects
RNA interference (RNAi) based methods are being developed for pest management. A few products for control of coleopteran pests are expected to be commercialized soon. However, variability in RNAi efficiency among insects is preventing the widespread use of this technology. In this study, we conducte...
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| Veröffentlicht in: | Scientific reports 2017-12, Vol.7 (1), p.17059-12, Article 17059 |
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| creator | Singh, Indrakant K. Singh, Satnam Mogilicherla, Kanakachari Shukla, Jayendra Nath Palli, Subba Reddy |
| description | RNA interference (RNAi) based methods are being developed for pest management. A few products for control of coleopteran pests are expected to be commercialized soon. However, variability in RNAi efficiency among insects is preventing the widespread use of this technology. In this study, we conducted research to identify reasons for variability in RNAi efficiency among thirty-seven (37) insects belonging to five orders. Studies on double-stranded RNA (dsRNA) degradation by dsRNases and processing of labeled dsRNA to siRNA showed that both dsRNA degradation and processing are variable among insects belonging to different orders as well as among different insect species within the same order. We identified homologs of key RNAi genes in the genomes of some of these insects and studied their domain architecture. These data suggest that dsRNA digestion by dsRNases and its processing to siRNAs in the cells are among the major factors contributing to differential RNAi efficiency reported among insects. |
| doi_str_mv | 10.1038/s41598-017-17134-2 |
| format | Article |
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A few products for control of coleopteran pests are expected to be commercialized soon. However, variability in RNAi efficiency among insects is preventing the widespread use of this technology. In this study, we conducted research to identify reasons for variability in RNAi efficiency among thirty-seven (37) insects belonging to five orders. Studies on double-stranded RNA (dsRNA) degradation by dsRNases and processing of labeled dsRNA to siRNA showed that both dsRNA degradation and processing are variable among insects belonging to different orders as well as among different insect species within the same order. We identified homologs of key RNAi genes in the genomes of some of these insects and studied their domain architecture. These data suggest that dsRNA digestion by dsRNases and its processing to siRNAs in the cells are among the major factors contributing to differential RNAi efficiency reported among insects.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-017-17134-2</identifier><identifier>PMID: 29213068</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>38 ; 38/89 ; 631/601/1466 ; 631/601/1737 ; Animals ; Body Fluids - metabolism ; Coleoptera - metabolism ; Comparative analysis ; Double-stranded RNA ; Efficiency ; Genomes ; Hemiptera - metabolism ; Humanities and Social Sciences ; Insect Proteins - metabolism ; Insecta - metabolism ; Insects ; Lepidoptera - metabolism ; multidisciplinary ; Pest control ; Pests ; Ribonuclease III - metabolism ; RNA processing ; RNA, Double-Stranded - administration & dosage ; RNA, Double-Stranded - metabolism ; RNA, Small Interfering - administration & dosage ; RNA, Small Interfering - metabolism ; RNA-mediated interference ; Science ; Science (multidisciplinary) ; siRNA</subject><ispartof>Scientific reports, 2017-12, Vol.7 (1), p.17059-12, Article 17059</ispartof><rights>The Author(s) 2017</rights><rights>2017. 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A few products for control of coleopteran pests are expected to be commercialized soon. However, variability in RNAi efficiency among insects is preventing the widespread use of this technology. In this study, we conducted research to identify reasons for variability in RNAi efficiency among thirty-seven (37) insects belonging to five orders. Studies on double-stranded RNA (dsRNA) degradation by dsRNases and processing of labeled dsRNA to siRNA showed that both dsRNA degradation and processing are variable among insects belonging to different orders as well as among different insect species within the same order. We identified homologs of key RNAi genes in the genomes of some of these insects and studied their domain architecture. 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A few products for control of coleopteran pests are expected to be commercialized soon. However, variability in RNAi efficiency among insects is preventing the widespread use of this technology. In this study, we conducted research to identify reasons for variability in RNAi efficiency among thirty-seven (37) insects belonging to five orders. Studies on double-stranded RNA (dsRNA) degradation by dsRNases and processing of labeled dsRNA to siRNA showed that both dsRNA degradation and processing are variable among insects belonging to different orders as well as among different insect species within the same order. We identified homologs of key RNAi genes in the genomes of some of these insects and studied their domain architecture. 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| subjects | 38 38/89 631/601/1466 631/601/1737 Animals Body Fluids - metabolism Coleoptera - metabolism Comparative analysis Double-stranded RNA Efficiency Genomes Hemiptera - metabolism Humanities and Social Sciences Insect Proteins - metabolism Insecta - metabolism Insects Lepidoptera - metabolism multidisciplinary Pest control Pests Ribonuclease III - metabolism RNA processing RNA, Double-Stranded - administration & dosage RNA, Double-Stranded - metabolism RNA, Small Interfering - administration & dosage RNA, Small Interfering - metabolism RNA-mediated interference Science Science (multidisciplinary) siRNA |
| title | Comparative analysis of double-stranded RNA degradation and processing in insects |
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