Mimicking Embedded Vasculature Structure for 3D Cancer on a Chip Approaches through Micromilling

The ability for cells to sense and respond to microenvironmental signals is influenced by their three dimensional (3D) surroundings, which includes the extracellular matrix (ECM). In the 3D environment, vascular structures supply cells with nutrients and oxygen thus affecting cell responses such as...

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Veröffentlicht in:Scientific reports 2017-12, Vol.7 (1), p.16724-8, Article 16724
Hauptverfasser: Wan, L., Skoko, J., Yu, J., Ozdoganlar, O. B., LeDuc, P. R., Neumann, C. A.
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container_issue 1
container_start_page 16724
container_title Scientific reports
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creator Wan, L.
Skoko, J.
Yu, J.
Ozdoganlar, O. B.
LeDuc, P. R.
Neumann, C. A.
description The ability for cells to sense and respond to microenvironmental signals is influenced by their three dimensional (3D) surroundings, which includes the extracellular matrix (ECM). In the 3D environment, vascular structures supply cells with nutrients and oxygen thus affecting cell responses such as motility. Interpretation of cell motility studies though is often restricted by the applied approaches such as 2D conventional soft lithography methods that have rectangular channel cross-sectional morphology. To better simulate cell responses to vascular supply in 3D, we developed a cell on a chip system with microfluidic channels with curved cross-sections embedded within a 3D collagen matrix that emulates anatomical vasculature more closely than inorganic polymers, thus to mimic a more physiologically relevant 3D cellular environment. To accomplish this, we constructed perfusable microfluidic channels by embedding sacrificial circular gelatin vascular templates in collagen, which were removed through temperature control. Motile breast cancer cells were pre-seeded into the collagen matrix and when presented with a controlled chemical stimulation from the artificial vasculature, they migrated towards the vasculature structure. We believe this innovative vascular 3D ECM system can be used to provide novel insights into cellular dynamics during multidirectional chemokineses and chemotaxis that exist in cancer and other diseases.
doi_str_mv 10.1038/s41598-017-16458-3
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subjects 13/62
631/1647/277
631/67/1347
Breast cancer
Breast Neoplasms - blood supply
Cancer
Cell Culture Techniques - instrumentation
Cell Line, Tumor
Cell Movement
Chemotaxis
Collagen
Cross-Sectional Studies
Cytology
Embedding
Extracellular matrix
Extracellular Matrix - chemistry
Female
Gelatin
Humanities and Social Sciences
Humans
Microfluidic Analytical Techniques
Microfluidics
Mimicry
Motility
multidisciplinary
Nutrients
Polymers
Science
Science (multidisciplinary)
Temperature control
Tissue Engineering - instrumentation
title Mimicking Embedded Vasculature Structure for 3D Cancer on a Chip Approaches through Micromilling
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