CXCL13 and TH1/Th2 cytokines in the serum and cerebrospinal fluid of neurosyphilis patients
Neurosyphilis is a chronic infectious disease with involvement of central nervous system infection by Treponema pallidum. This study was to investigate the contents of B lymphocyte chemokine 1 (BLC-1/chemokine [C-X-C motif] ligand 13), Th1 cytokines (Interleukin [IL]-2, IL-12, and Interferon [IFN]-γ...
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| Veröffentlicht in: | Medicine (Baltimore) 2017-11, Vol.96 (47), p.e8850-e8850 |
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| creator | Yan, Yongxing Wang, Jun Qu, Bin Zhang, Yan Wei, Yingnan Liu, Huili Wu, Chunli |
| description | Neurosyphilis is a chronic infectious disease with involvement of central nervous system infection by Treponema pallidum. This study was to investigate the contents of B lymphocyte chemokine 1 (BLC-1/chemokine [C-X-C motif] ligand 13), Th1 cytokines (Interleukin [IL]-2, IL-12, and Interferon [IFN]-γ), and Th2 cytokines (IL-6 and IL-10) in serum and cerebrospinal fluid (CSF) of HIV-negative patients with neurosyphilis before and after treatment, aiming to elucidate roles of CXCL13 and Th1/Th2 cytokines in immune response to and pathogenesis of neurosyphilis.Enzyme-linked immunosorbent assay was employed to detect the contents of CXCL13, IL-2, IL-12, IFN-γ, IL-6, and IL-10 in serum and CSF of 47 HIV-negative patients with neurosyphilis, 36 syphilis patients without neurological involvement and 23 controls (noninfectious intracranial disease) before, 3 and 12 months after treatment with high dose penicillin.Results showed that there was no significant difference in blood CXCL13 content among 3 groups (P > .05); CSF CXCL13 content in neurosyphilis patients was significantly higher than in other 2 groups (P |
| doi_str_mv | 10.1097/MD.0000000000008850 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5708994</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>29381995</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4506-fa8632b3e516ccb2dea89a16a7491720b27544a0da51e44ea19ba8307207ca5d3</originalsourceid><addsrcrecordid>eNpdkN1q20AQhZeS0jhpnyBQ9gVk769We1MITlsHHHLjQiAXy0gaVZvIktiVYvz2lePWpJmbgZlzvmEOIVeczTmzZnF3M2dvKss0-0BmXMs00TZVZ2TGmNCJsUadk4sYnxjj0gj1iZwLKzNurZ6Rx-XDcs0lhbakmxVfbGpBi_3QPfsWI_UtHWqkEcO4fZUUGDAPXex9Cw2tmtGXtKtoi-M03Pe1b3ykPQwe2yF-Jh8raCJ--dsvya8f3zfLVbK-_3m7vF4nhdIsTSrIUilyiZqnRZGLEiGzwFMwynIjWC6MVgpYCZqjUgjc5pBJNq1MAbqUl-TbkduP-RbLYrodoHF98FsIe9eBd_9vWl-7392L04Zl1qoJII-AYvoiBqxOXs7cIWt3d-PeZz25vr49e_L8C3cSqKNg1zUDhvjcjDsMrkZohvqVp40ViWDccC4MSw7kVP4B-y6KPw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>CXCL13 and TH1/Th2 cytokines in the serum and cerebrospinal fluid of neurosyphilis patients</title><source>Wolters Kluwer Open Health</source><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>IngentaConnect Free/Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Yan, Yongxing ; Wang, Jun ; Qu, Bin ; Zhang, Yan ; Wei, Yingnan ; Liu, Huili ; Wu, Chunli</creator><creatorcontrib>Yan, Yongxing ; Wang, Jun ; Qu, Bin ; Zhang, Yan ; Wei, Yingnan ; Liu, Huili ; Wu, Chunli</creatorcontrib><description>Neurosyphilis is a chronic infectious disease with involvement of central nervous system infection by Treponema pallidum. This study was to investigate the contents of B lymphocyte chemokine 1 (BLC-1/chemokine [C-X-C motif] ligand 13), Th1 cytokines (Interleukin [IL]-2, IL-12, and Interferon [IFN]-γ), and Th2 cytokines (IL-6 and IL-10) in serum and cerebrospinal fluid (CSF) of HIV-negative patients with neurosyphilis before and after treatment, aiming to elucidate roles of CXCL13 and Th1/Th2 cytokines in immune response to and pathogenesis of neurosyphilis.Enzyme-linked immunosorbent assay was employed to detect the contents of CXCL13, IL-2, IL-12, IFN-γ, IL-6, and IL-10 in serum and CSF of 47 HIV-negative patients with neurosyphilis, 36 syphilis patients without neurological involvement and 23 controls (noninfectious intracranial disease) before, 3 and 12 months after treatment with high dose penicillin.Results showed that there was no significant difference in blood CXCL13 content among 3 groups (P > .05); CSF CXCL13 content in neurosyphilis patients was significantly higher than in other 2 groups (P < .001), and positively related to leucocyte count, protein concentration, and IgG index. IL-6 and IL-10 contents of the serum and CSF in neurosyphilis patients were markedly higher than in other 2 groups (P < .05 or .01), but IL-2, IL-12, and IFN-γ of the serum and CSF were significantly lower than in other 2 groups (P < .05 or .01). The IL-6, IL-10, IL-2, IL-12, and IFN-γ contents of the serum and CSF were comparable between control group and syphilis group (P > .05). CSF CXCL13 content was positively related with IL-6 and IL-10 content, while negatively related to IL-12 content in neurosyphilis patients. CSF IL-6 content was negatively related with IL-12 content. In neurosyphilis patients, the CSF CXCL13 content reduced significantly at 3 and 12 months (P < .001), the CSF IL-2 and IL-12 contents increased significantly at 12 months, and CSF IL-6 contents reduced significantly at 12 months after treatment (P < .05 or .01).It is concluded that neurosyphilis patients did not have normal immune function. CXCL13 and Th1/Th2 cytokines are involved in the immune response of neurosyphilis patients. CSF CXCL13 and Th1/Th2 cytokines contents may be used for the diagnosis and evaluation of therapeutic efficacy of neurosyphilis.</description><identifier>ISSN: 0025-7974</identifier><identifier>EISSN: 1536-5964</identifier><identifier>DOI: 10.1097/MD.0000000000008850</identifier><identifier>PMID: 29381995</identifier><language>eng</language><publisher>United States: The Authors. Published by Wolters Kluwer Health, Inc. All rights reserved</publisher><subject>Adult ; Aged ; Anti-Bacterial Agents - therapeutic use ; Case-Control Studies ; Chemokine CXCL13 - analysis ; Cytokines - analysis ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Interferon-gamma - analysis ; Interleukin-10 - analysis ; Interleukin-12 - analysis ; Interleukin-2 - analysis ; Interleukin-6 - analysis ; Male ; Middle Aged ; Neurosyphilis - blood ; Neurosyphilis - cerebrospinal fluid ; Neurosyphilis - drug therapy ; Observational Study ; Penicillins - therapeutic use ; Syphilis - blood ; Syphilis - cerebrospinal fluid ; Syphilis - drug therapy ; Th1-Th2 Balance - drug effects ; Young Adult</subject><ispartof>Medicine (Baltimore), 2017-11, Vol.96 (47), p.e8850-e8850</ispartof><rights>The Authors. Published by Wolters Kluwer Health, Inc. All rights reserved.</rights><rights>Copyright © 2017 The Authors. Published by Wolters Kluwer Health, Inc. All rights reserved.</rights><rights>Copyright © 2017 the Author(s). Published by Wolters Kluwer Health, Inc. 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4506-fa8632b3e516ccb2dea89a16a7491720b27544a0da51e44ea19ba8307207ca5d3</citedby><cites>FETCH-LOGICAL-c4506-fa8632b3e516ccb2dea89a16a7491720b27544a0da51e44ea19ba8307207ca5d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5708994/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5708994/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29381995$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yan, Yongxing</creatorcontrib><creatorcontrib>Wang, Jun</creatorcontrib><creatorcontrib>Qu, Bin</creatorcontrib><creatorcontrib>Zhang, Yan</creatorcontrib><creatorcontrib>Wei, Yingnan</creatorcontrib><creatorcontrib>Liu, Huili</creatorcontrib><creatorcontrib>Wu, Chunli</creatorcontrib><title>CXCL13 and TH1/Th2 cytokines in the serum and cerebrospinal fluid of neurosyphilis patients</title><title>Medicine (Baltimore)</title><addtitle>Medicine (Baltimore)</addtitle><description>Neurosyphilis is a chronic infectious disease with involvement of central nervous system infection by Treponema pallidum. This study was to investigate the contents of B lymphocyte chemokine 1 (BLC-1/chemokine [C-X-C motif] ligand 13), Th1 cytokines (Interleukin [IL]-2, IL-12, and Interferon [IFN]-γ), and Th2 cytokines (IL-6 and IL-10) in serum and cerebrospinal fluid (CSF) of HIV-negative patients with neurosyphilis before and after treatment, aiming to elucidate roles of CXCL13 and Th1/Th2 cytokines in immune response to and pathogenesis of neurosyphilis.Enzyme-linked immunosorbent assay was employed to detect the contents of CXCL13, IL-2, IL-12, IFN-γ, IL-6, and IL-10 in serum and CSF of 47 HIV-negative patients with neurosyphilis, 36 syphilis patients without neurological involvement and 23 controls (noninfectious intracranial disease) before, 3 and 12 months after treatment with high dose penicillin.Results showed that there was no significant difference in blood CXCL13 content among 3 groups (P > .05); CSF CXCL13 content in neurosyphilis patients was significantly higher than in other 2 groups (P < .001), and positively related to leucocyte count, protein concentration, and IgG index. IL-6 and IL-10 contents of the serum and CSF in neurosyphilis patients were markedly higher than in other 2 groups (P < .05 or .01), but IL-2, IL-12, and IFN-γ of the serum and CSF were significantly lower than in other 2 groups (P < .05 or .01). The IL-6, IL-10, IL-2, IL-12, and IFN-γ contents of the serum and CSF were comparable between control group and syphilis group (P > .05). CSF CXCL13 content was positively related with IL-6 and IL-10 content, while negatively related to IL-12 content in neurosyphilis patients. CSF IL-6 content was negatively related with IL-12 content. In neurosyphilis patients, the CSF CXCL13 content reduced significantly at 3 and 12 months (P < .001), the CSF IL-2 and IL-12 contents increased significantly at 12 months, and CSF IL-6 contents reduced significantly at 12 months after treatment (P < .05 or .01).It is concluded that neurosyphilis patients did not have normal immune function. CXCL13 and Th1/Th2 cytokines are involved in the immune response of neurosyphilis patients. CSF CXCL13 and Th1/Th2 cytokines contents may be used for the diagnosis and evaluation of therapeutic efficacy of neurosyphilis.</description><subject>Adult</subject><subject>Aged</subject><subject>Anti-Bacterial Agents - therapeutic use</subject><subject>Case-Control Studies</subject><subject>Chemokine CXCL13 - analysis</subject><subject>Cytokines - analysis</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Humans</subject><subject>Interferon-gamma - analysis</subject><subject>Interleukin-10 - analysis</subject><subject>Interleukin-12 - analysis</subject><subject>Interleukin-2 - analysis</subject><subject>Interleukin-6 - analysis</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Neurosyphilis - blood</subject><subject>Neurosyphilis - cerebrospinal fluid</subject><subject>Neurosyphilis - drug therapy</subject><subject>Observational Study</subject><subject>Penicillins - therapeutic use</subject><subject>Syphilis - blood</subject><subject>Syphilis - cerebrospinal fluid</subject><subject>Syphilis - drug therapy</subject><subject>Th1-Th2 Balance - drug effects</subject><subject>Young Adult</subject><issn>0025-7974</issn><issn>1536-5964</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkN1q20AQhZeS0jhpnyBQ9gVk769We1MITlsHHHLjQiAXy0gaVZvIktiVYvz2lePWpJmbgZlzvmEOIVeczTmzZnF3M2dvKss0-0BmXMs00TZVZ2TGmNCJsUadk4sYnxjj0gj1iZwLKzNurZ6Rx-XDcs0lhbakmxVfbGpBi_3QPfsWI_UtHWqkEcO4fZUUGDAPXex9Cw2tmtGXtKtoi-M03Pe1b3ykPQwe2yF-Jh8raCJ--dsvya8f3zfLVbK-_3m7vF4nhdIsTSrIUilyiZqnRZGLEiGzwFMwynIjWC6MVgpYCZqjUgjc5pBJNq1MAbqUl-TbkduP-RbLYrodoHF98FsIe9eBd_9vWl-7392L04Zl1qoJII-AYvoiBqxOXs7cIWt3d-PeZz25vr49e_L8C3cSqKNg1zUDhvjcjDsMrkZohvqVp40ViWDccC4MSw7kVP4B-y6KPw</recordid><startdate>20171101</startdate><enddate>20171101</enddate><creator>Yan, Yongxing</creator><creator>Wang, Jun</creator><creator>Qu, Bin</creator><creator>Zhang, Yan</creator><creator>Wei, Yingnan</creator><creator>Liu, Huili</creator><creator>Wu, Chunli</creator><general>The Authors. Published by Wolters Kluwer Health, Inc. All rights reserved</general><general>Wolters Kluwer Health</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20171101</creationdate><title>CXCL13 and TH1/Th2 cytokines in the serum and cerebrospinal fluid of neurosyphilis patients</title><author>Yan, Yongxing ; Wang, Jun ; Qu, Bin ; Zhang, Yan ; Wei, Yingnan ; Liu, Huili ; Wu, Chunli</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4506-fa8632b3e516ccb2dea89a16a7491720b27544a0da51e44ea19ba8307207ca5d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Anti-Bacterial Agents - therapeutic use</topic><topic>Case-Control Studies</topic><topic>Chemokine CXCL13 - analysis</topic><topic>Cytokines - analysis</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Humans</topic><topic>Interferon-gamma - analysis</topic><topic>Interleukin-10 - analysis</topic><topic>Interleukin-12 - analysis</topic><topic>Interleukin-2 - analysis</topic><topic>Interleukin-6 - analysis</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Neurosyphilis - blood</topic><topic>Neurosyphilis - cerebrospinal fluid</topic><topic>Neurosyphilis - drug therapy</topic><topic>Observational Study</topic><topic>Penicillins - therapeutic use</topic><topic>Syphilis - blood</topic><topic>Syphilis - cerebrospinal fluid</topic><topic>Syphilis - drug therapy</topic><topic>Th1-Th2 Balance - drug effects</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yan, Yongxing</creatorcontrib><creatorcontrib>Wang, Jun</creatorcontrib><creatorcontrib>Qu, Bin</creatorcontrib><creatorcontrib>Zhang, Yan</creatorcontrib><creatorcontrib>Wei, Yingnan</creatorcontrib><creatorcontrib>Liu, Huili</creatorcontrib><creatorcontrib>Wu, Chunli</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Medicine (Baltimore)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yan, Yongxing</au><au>Wang, Jun</au><au>Qu, Bin</au><au>Zhang, Yan</au><au>Wei, Yingnan</au><au>Liu, Huili</au><au>Wu, Chunli</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CXCL13 and TH1/Th2 cytokines in the serum and cerebrospinal fluid of neurosyphilis patients</atitle><jtitle>Medicine (Baltimore)</jtitle><addtitle>Medicine (Baltimore)</addtitle><date>2017-11-01</date><risdate>2017</risdate><volume>96</volume><issue>47</issue><spage>e8850</spage><epage>e8850</epage><pages>e8850-e8850</pages><issn>0025-7974</issn><eissn>1536-5964</eissn><abstract>Neurosyphilis is a chronic infectious disease with involvement of central nervous system infection by Treponema pallidum. This study was to investigate the contents of B lymphocyte chemokine 1 (BLC-1/chemokine [C-X-C motif] ligand 13), Th1 cytokines (Interleukin [IL]-2, IL-12, and Interferon [IFN]-γ), and Th2 cytokines (IL-6 and IL-10) in serum and cerebrospinal fluid (CSF) of HIV-negative patients with neurosyphilis before and after treatment, aiming to elucidate roles of CXCL13 and Th1/Th2 cytokines in immune response to and pathogenesis of neurosyphilis.Enzyme-linked immunosorbent assay was employed to detect the contents of CXCL13, IL-2, IL-12, IFN-γ, IL-6, and IL-10 in serum and CSF of 47 HIV-negative patients with neurosyphilis, 36 syphilis patients without neurological involvement and 23 controls (noninfectious intracranial disease) before, 3 and 12 months after treatment with high dose penicillin.Results showed that there was no significant difference in blood CXCL13 content among 3 groups (P > .05); CSF CXCL13 content in neurosyphilis patients was significantly higher than in other 2 groups (P < .001), and positively related to leucocyte count, protein concentration, and IgG index. IL-6 and IL-10 contents of the serum and CSF in neurosyphilis patients were markedly higher than in other 2 groups (P < .05 or .01), but IL-2, IL-12, and IFN-γ of the serum and CSF were significantly lower than in other 2 groups (P < .05 or .01). The IL-6, IL-10, IL-2, IL-12, and IFN-γ contents of the serum and CSF were comparable between control group and syphilis group (P > .05). CSF CXCL13 content was positively related with IL-6 and IL-10 content, while negatively related to IL-12 content in neurosyphilis patients. CSF IL-6 content was negatively related with IL-12 content. In neurosyphilis patients, the CSF CXCL13 content reduced significantly at 3 and 12 months (P < .001), the CSF IL-2 and IL-12 contents increased significantly at 12 months, and CSF IL-6 contents reduced significantly at 12 months after treatment (P < .05 or .01).It is concluded that neurosyphilis patients did not have normal immune function. CXCL13 and Th1/Th2 cytokines are involved in the immune response of neurosyphilis patients. CSF CXCL13 and Th1/Th2 cytokines contents may be used for the diagnosis and evaluation of therapeutic efficacy of neurosyphilis.</abstract><cop>United States</cop><pub>The Authors. Published by Wolters Kluwer Health, Inc. All rights reserved</pub><pmid>29381995</pmid><doi>10.1097/MD.0000000000008850</doi><oa>free_for_read</oa></addata></record> |
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| ispartof | Medicine (Baltimore), 2017-11, Vol.96 (47), p.e8850-e8850 |
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| source | Wolters Kluwer Open Health; MEDLINE; DOAJ Directory of Open Access Journals; IngentaConnect Free/Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Adult Aged Anti-Bacterial Agents - therapeutic use Case-Control Studies Chemokine CXCL13 - analysis Cytokines - analysis Enzyme-Linked Immunosorbent Assay Female Humans Interferon-gamma - analysis Interleukin-10 - analysis Interleukin-12 - analysis Interleukin-2 - analysis Interleukin-6 - analysis Male Middle Aged Neurosyphilis - blood Neurosyphilis - cerebrospinal fluid Neurosyphilis - drug therapy Observational Study Penicillins - therapeutic use Syphilis - blood Syphilis - cerebrospinal fluid Syphilis - drug therapy Th1-Th2 Balance - drug effects Young Adult |
| title | CXCL13 and TH1/Th2 cytokines in the serum and cerebrospinal fluid of neurosyphilis patients |
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