New Insights into Phasmatodea Chromosomes
Currently, approximately 3000 species of stick insects are known; however, chromosome numbers, which range between 21 and 88, are known for only a few of these insects. Also, centromere banding staining (C-banding) patterns were described for fewer than 10 species, and fluorescence in situ hybridiza...
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| Veröffentlicht in: | Genes 2017-11, Vol.8 (11), p.327 |
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| creator | Liehr, Thomas Buleu, Olesya Karamysheva, Tatyana Bugrov, Alexander Rubtsov, Nikolai |
| description | Currently, approximately 3000 species of stick insects are known; however, chromosome numbers, which range between 21 and 88, are known for only a few of these insects. Also, centromere banding staining (C-banding) patterns were described for fewer than 10 species, and fluorescence in situ hybridization (FISH) was applied exclusively in two
species. Interestingly, 10-25% of stick insects (Phasmatodea) are obligatory or facultative parthenogenetic. As clonal and/or bisexual reproduction can affect chromosomal evolution, stick insect karyotypes need to be studied more intensely. Chromosome preparation from embryos of five Phasmatodea species (
,
,
,
, and
) from four families were studied here by C-banding and FISH applying ribosomal deoxyribonucleic acid (rDNA) and telomeric repeat probes. For three species, data on chromosome numbers and structure were obtained here for the first time, i.e.,
,
, and
. Large C-positive regions enriched with rDNA were identified in all five studied, distantly related species. Some of these C-positive blocks were enriched for telomeric repeats, as well. Chromosomal evolution of stick insects is characterized by variations in chromosome numbers as well as transposition and amplification of repetitive DNA sequences. Here, the first steps were made towards identification of individual chromosomes in Phasmatodea. |
| doi_str_mv | 10.3390/genes8110327 |
| format | Article |
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species. Interestingly, 10-25% of stick insects (Phasmatodea) are obligatory or facultative parthenogenetic. As clonal and/or bisexual reproduction can affect chromosomal evolution, stick insect karyotypes need to be studied more intensely. Chromosome preparation from embryos of five Phasmatodea species (
,
,
,
, and
) from four families were studied here by C-banding and FISH applying ribosomal deoxyribonucleic acid (rDNA) and telomeric repeat probes. For three species, data on chromosome numbers and structure were obtained here for the first time, i.e.,
,
, and
. Large C-positive regions enriched with rDNA were identified in all five studied, distantly related species. Some of these C-positive blocks were enriched for telomeric repeats, as well. Chromosomal evolution of stick insects is characterized by variations in chromosome numbers as well as transposition and amplification of repetitive DNA sequences. Here, the first steps were made towards identification of individual chromosomes in Phasmatodea.</description><identifier>ISSN: 2073-4425</identifier><identifier>EISSN: 2073-4425</identifier><identifier>DOI: 10.3390/genes8110327</identifier><identifier>PMID: 29149047</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Bisexuality ; Chromosomes ; Deoxyribonucleic acid ; DNA ; DNA probes ; Embryos ; Fluorescence in situ hybridization ; Insects ; Karyotypes ; Nucleotide sequence ; Phasmatodea ; Species ; Transposition</subject><ispartof>Genes, 2017-11, Vol.8 (11), p.327</ispartof><rights>Copyright MDPI AG 2017</rights><rights>2017 by the authors. 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-b29abe05f4f258b828c47373f565cfad1cbcd672b794bad7dc4374a86b0f39c23</citedby><cites>FETCH-LOGICAL-c412t-b29abe05f4f258b828c47373f565cfad1cbcd672b794bad7dc4374a86b0f39c23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5704240/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5704240/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29149047$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liehr, Thomas</creatorcontrib><creatorcontrib>Buleu, Olesya</creatorcontrib><creatorcontrib>Karamysheva, Tatyana</creatorcontrib><creatorcontrib>Bugrov, Alexander</creatorcontrib><creatorcontrib>Rubtsov, Nikolai</creatorcontrib><title>New Insights into Phasmatodea Chromosomes</title><title>Genes</title><addtitle>Genes (Basel)</addtitle><description>Currently, approximately 3000 species of stick insects are known; however, chromosome numbers, which range between 21 and 88, are known for only a few of these insects. Also, centromere banding staining (C-banding) patterns were described for fewer than 10 species, and fluorescence in situ hybridization (FISH) was applied exclusively in two
species. Interestingly, 10-25% of stick insects (Phasmatodea) are obligatory or facultative parthenogenetic. As clonal and/or bisexual reproduction can affect chromosomal evolution, stick insect karyotypes need to be studied more intensely. Chromosome preparation from embryos of five Phasmatodea species (
,
,
,
, and
) from four families were studied here by C-banding and FISH applying ribosomal deoxyribonucleic acid (rDNA) and telomeric repeat probes. For three species, data on chromosome numbers and structure were obtained here for the first time, i.e.,
,
, and
. Large C-positive regions enriched with rDNA were identified in all five studied, distantly related species. Some of these C-positive blocks were enriched for telomeric repeats, as well. Chromosomal evolution of stick insects is characterized by variations in chromosome numbers as well as transposition and amplification of repetitive DNA sequences. Here, the first steps were made towards identification of individual chromosomes in Phasmatodea.</description><subject>Bisexuality</subject><subject>Chromosomes</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA probes</subject><subject>Embryos</subject><subject>Fluorescence in situ hybridization</subject><subject>Insects</subject><subject>Karyotypes</subject><subject>Nucleotide sequence</subject><subject>Phasmatodea</subject><subject>Species</subject><subject>Transposition</subject><issn>2073-4425</issn><issn>2073-4425</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkc1Lw0AQxRdRbKm9eZaAFwWjs1_Z5CJI8QuKetDzsrvZNClJtu4miv-9kdZSncsMzI_HvHkIHWO4pDSDq4VtbUgxBkrEHhoTEDRmjPD9nXmEpiEsYSgGBIAfohHJMMuAiTE6f7Kf0WMbqkXZhahqOxe9lCo0qnO5VdGs9K5xwTU2HKGDQtXBTjd9gt7ubl9nD_H8-f5xdjOPDcOkizXJlLbAC1YQnuqUpIYJKmjBE24KlWOjTZ4IokXGtMpFbhgVTKWJhoJmhtAJul7rrnrd2NzYtvOqlitfNcp_Sacq-XfTVqVcuA_JBTDCYBA42wh4997b0MmmCsbWtWqt64PEWZIQSinnA3r6D1263reDvYESKQDBlA3UxZoy3oXgbbE9BoP8iUHuxjDgJ7sGtvDv0-k3FrCC8Q</recordid><startdate>20171117</startdate><enddate>20171117</enddate><creator>Liehr, Thomas</creator><creator>Buleu, Olesya</creator><creator>Karamysheva, Tatyana</creator><creator>Bugrov, Alexander</creator><creator>Rubtsov, Nikolai</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20171117</creationdate><title>New Insights into Phasmatodea Chromosomes</title><author>Liehr, Thomas ; Buleu, Olesya ; Karamysheva, Tatyana ; Bugrov, Alexander ; Rubtsov, Nikolai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-b29abe05f4f258b828c47373f565cfad1cbcd672b794bad7dc4374a86b0f39c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Bisexuality</topic><topic>Chromosomes</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA probes</topic><topic>Embryos</topic><topic>Fluorescence in situ hybridization</topic><topic>Insects</topic><topic>Karyotypes</topic><topic>Nucleotide sequence</topic><topic>Phasmatodea</topic><topic>Species</topic><topic>Transposition</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liehr, Thomas</creatorcontrib><creatorcontrib>Buleu, Olesya</creatorcontrib><creatorcontrib>Karamysheva, Tatyana</creatorcontrib><creatorcontrib>Bugrov, Alexander</creatorcontrib><creatorcontrib>Rubtsov, Nikolai</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liehr, Thomas</au><au>Buleu, Olesya</au><au>Karamysheva, Tatyana</au><au>Bugrov, Alexander</au><au>Rubtsov, Nikolai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>New Insights into Phasmatodea Chromosomes</atitle><jtitle>Genes</jtitle><addtitle>Genes (Basel)</addtitle><date>2017-11-17</date><risdate>2017</risdate><volume>8</volume><issue>11</issue><spage>327</spage><pages>327-</pages><issn>2073-4425</issn><eissn>2073-4425</eissn><abstract>Currently, approximately 3000 species of stick insects are known; however, chromosome numbers, which range between 21 and 88, are known for only a few of these insects. Also, centromere banding staining (C-banding) patterns were described for fewer than 10 species, and fluorescence in situ hybridization (FISH) was applied exclusively in two
species. Interestingly, 10-25% of stick insects (Phasmatodea) are obligatory or facultative parthenogenetic. As clonal and/or bisexual reproduction can affect chromosomal evolution, stick insect karyotypes need to be studied more intensely. Chromosome preparation from embryos of five Phasmatodea species (
,
,
,
, and
) from four families were studied here by C-banding and FISH applying ribosomal deoxyribonucleic acid (rDNA) and telomeric repeat probes. For three species, data on chromosome numbers and structure were obtained here for the first time, i.e.,
,
, and
. Large C-positive regions enriched with rDNA were identified in all five studied, distantly related species. Some of these C-positive blocks were enriched for telomeric repeats, as well. Chromosomal evolution of stick insects is characterized by variations in chromosome numbers as well as transposition and amplification of repetitive DNA sequences. Here, the first steps were made towards identification of individual chromosomes in Phasmatodea.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>29149047</pmid><doi>10.3390/genes8110327</doi><oa>free_for_read</oa></addata></record> |
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| source | PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Bisexuality Chromosomes Deoxyribonucleic acid DNA DNA probes Embryos Fluorescence in situ hybridization Insects Karyotypes Nucleotide sequence Phasmatodea Species Transposition |
| title | New Insights into Phasmatodea Chromosomes |
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