Detection and differentiation of HIV-2 using the point-of-care Alere q HIV-1/2 Detect nucleic acid test
•The Alere q HIV-1/2 Detect is a rapid point-of-care HIV nucleic acid test.•It can qualitatively detect and differentiate HIV-1 and HIV-2 in whole blood or plasma samples. The Alere q HIV-1/2 Detect test (Alere Detect) is a rapid point-of-care (POC) nucleic acid test (NAT) that can detect and differ...
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| Veröffentlicht in: | Journal of clinical virology 2017-12, Vol.97, p.22-25 |
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| creator | Chang, Ming Steinmetzer, Katrin Raugi, Dana N. Smith, Robert A. Ba, Selly Sall, Fatima Seydi, Moussa Niang, Alassane Sall, ElHadji Ibrahima Cisse, Ousseynou Rödel, Katja Coombs, Robert W. Gottlieb, Geoffrey S. |
| description | •The Alere q HIV-1/2 Detect is a rapid point-of-care HIV nucleic acid test.•It can qualitatively detect and differentiate HIV-1 and HIV-2 in whole blood or plasma samples.
The Alere q HIV-1/2 Detect test (Alere Detect) is a rapid point-of-care (POC) nucleic acid test (NAT) that can detect and differentiate HIV-1 and HIV-2 in 25-μL whole blood or plasma samples. The Alere Detect test has been validated for early infant diagnosis of HIV-1 infection, and it is the only POC NAT device currently known to detect HIV-2, which is endemic in West Africa.
To evaluate the sensitivity detecting HIV-2 RNA and the differential performance of the Alere Detect.
Plasma samples from non-HIV (n=4), HIV-1 (n=22), HIV-2 (n=111; 29 Group A, 2 Group B) and HIV-1/HIV-2 dually-seropositive (n=8) participants in Senegal and the United States and HIV-2 reference strains (3 Group A, 1 Group B) were tested by Alere Detect, Abbott RealTime HIV-1 and the University of Washington HIV-2 RNA quantitative (UW HIV-2) assays.
The Alere Detect correctly differentiated between HIV-1 and HIV-2 in all 80 (100%) patient samples with detectable HIV RNA (n=20 HIV-1, 60 HIV-2). The overall HIV-2 detection concordance between Alere Detect and the UW HIV-2 assay was 68% (54/80); the concordance improved to 100% (30/30) for samples with HIV-2 RNA >300copies/mL. Neither assay detected HIV-2 RNA in 31 of 111 HIV-2 seropositive samples.
The Alere Detect test is a novel device detecting HIV RNA in clinical samples, and differentiating HIV-1 and HIV-2 with a high level of specificity. It has the potential for use as a rapid HIV-2 NAT-based diagnosis tool in resource-limited settings and to confirm HIV-2 infection for the CDC 4th generation HIV-1/2 diagnostic algorithm. |
| doi_str_mv | 10.1016/j.jcv.2017.10.013 |
| format | Article |
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The Alere q HIV-1/2 Detect test (Alere Detect) is a rapid point-of-care (POC) nucleic acid test (NAT) that can detect and differentiate HIV-1 and HIV-2 in 25-μL whole blood or plasma samples. The Alere Detect test has been validated for early infant diagnosis of HIV-1 infection, and it is the only POC NAT device currently known to detect HIV-2, which is endemic in West Africa.
To evaluate the sensitivity detecting HIV-2 RNA and the differential performance of the Alere Detect.
Plasma samples from non-HIV (n=4), HIV-1 (n=22), HIV-2 (n=111; 29 Group A, 2 Group B) and HIV-1/HIV-2 dually-seropositive (n=8) participants in Senegal and the United States and HIV-2 reference strains (3 Group A, 1 Group B) were tested by Alere Detect, Abbott RealTime HIV-1 and the University of Washington HIV-2 RNA quantitative (UW HIV-2) assays.
The Alere Detect correctly differentiated between HIV-1 and HIV-2 in all 80 (100%) patient samples with detectable HIV RNA (n=20 HIV-1, 60 HIV-2). The overall HIV-2 detection concordance between Alere Detect and the UW HIV-2 assay was 68% (54/80); the concordance improved to 100% (30/30) for samples with HIV-2 RNA >300copies/mL. Neither assay detected HIV-2 RNA in 31 of 111 HIV-2 seropositive samples.
The Alere Detect test is a novel device detecting HIV RNA in clinical samples, and differentiating HIV-1 and HIV-2 with a high level of specificity. It has the potential for use as a rapid HIV-2 NAT-based diagnosis tool in resource-limited settings and to confirm HIV-2 infection for the CDC 4th generation HIV-1/2 diagnostic algorithm.</description><identifier>ISSN: 1386-6532</identifier><identifier>EISSN: 1873-5967</identifier><identifier>DOI: 10.1016/j.jcv.2017.10.013</identifier><identifier>PMID: 29096389</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Alere q HIV-1/2 Detect ; Diagnosis ; HIV-2 ; Nucleic acid test ; Point-of-care ; Qualitative</subject><ispartof>Journal of clinical virology, 2017-12, Vol.97, p.22-25</ispartof><rights>2017 Elsevier B.V.</rights><rights>Copyright © 2017 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-1e07299bd33884c3d4e2e38c8d754e584f91705dfb5a7aff967ab896d14e338c3</citedby><cites>FETCH-LOGICAL-c451t-1e07299bd33884c3d4e2e38c8d754e584f91705dfb5a7aff967ab896d14e338c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1386653217302895$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29096389$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chang, Ming</creatorcontrib><creatorcontrib>Steinmetzer, Katrin</creatorcontrib><creatorcontrib>Raugi, Dana N.</creatorcontrib><creatorcontrib>Smith, Robert A.</creatorcontrib><creatorcontrib>Ba, Selly</creatorcontrib><creatorcontrib>Sall, Fatima</creatorcontrib><creatorcontrib>Seydi, Moussa</creatorcontrib><creatorcontrib>Niang, Alassane</creatorcontrib><creatorcontrib>Sall, ElHadji Ibrahima</creatorcontrib><creatorcontrib>Cisse, Ousseynou</creatorcontrib><creatorcontrib>Rödel, Katja</creatorcontrib><creatorcontrib>Coombs, Robert W.</creatorcontrib><creatorcontrib>Gottlieb, Geoffrey S.</creatorcontrib><title>Detection and differentiation of HIV-2 using the point-of-care Alere q HIV-1/2 Detect nucleic acid test</title><title>Journal of clinical virology</title><addtitle>J Clin Virol</addtitle><description>•The Alere q HIV-1/2 Detect is a rapid point-of-care HIV nucleic acid test.•It can qualitatively detect and differentiate HIV-1 and HIV-2 in whole blood or plasma samples.
The Alere q HIV-1/2 Detect test (Alere Detect) is a rapid point-of-care (POC) nucleic acid test (NAT) that can detect and differentiate HIV-1 and HIV-2 in 25-μL whole blood or plasma samples. The Alere Detect test has been validated for early infant diagnosis of HIV-1 infection, and it is the only POC NAT device currently known to detect HIV-2, which is endemic in West Africa.
To evaluate the sensitivity detecting HIV-2 RNA and the differential performance of the Alere Detect.
Plasma samples from non-HIV (n=4), HIV-1 (n=22), HIV-2 (n=111; 29 Group A, 2 Group B) and HIV-1/HIV-2 dually-seropositive (n=8) participants in Senegal and the United States and HIV-2 reference strains (3 Group A, 1 Group B) were tested by Alere Detect, Abbott RealTime HIV-1 and the University of Washington HIV-2 RNA quantitative (UW HIV-2) assays.
The Alere Detect correctly differentiated between HIV-1 and HIV-2 in all 80 (100%) patient samples with detectable HIV RNA (n=20 HIV-1, 60 HIV-2). The overall HIV-2 detection concordance between Alere Detect and the UW HIV-2 assay was 68% (54/80); the concordance improved to 100% (30/30) for samples with HIV-2 RNA >300copies/mL. Neither assay detected HIV-2 RNA in 31 of 111 HIV-2 seropositive samples.
The Alere Detect test is a novel device detecting HIV RNA in clinical samples, and differentiating HIV-1 and HIV-2 with a high level of specificity. It has the potential for use as a rapid HIV-2 NAT-based diagnosis tool in resource-limited settings and to confirm HIV-2 infection for the CDC 4th generation HIV-1/2 diagnostic algorithm.</description><subject>Alere q HIV-1/2 Detect</subject><subject>Diagnosis</subject><subject>HIV-2</subject><subject>Nucleic acid test</subject><subject>Point-of-care</subject><subject>Qualitative</subject><issn>1386-6532</issn><issn>1873-5967</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNp9kctuEzEUhi0Eohf6AGyQl2wm9XVsCwmp6oVWqsQG2FqOfZw6moxTeyYSb4_TtBVsWNk-_s5_Lj9CHylZUEL78_Vi7XcLRqhq7wWh_A06plrxTppevW13rvuul5wdoZNa14RQyYV6j46YIabn2hyj1RVM4KeUR-zGgEOKEQqMU3JPsRzx7d2vjuG5pnGFpwfA25zGqcux864Avhgajh-fKHrO8EEOj7MfIHnsfAp4gjp9QO-iGyqcPZ-n6OfN9Y_L2-7--7e7y4v7zgtJp44CUcyYZeBca-F5EMCAa6-DkgKkFtFQRWSIS-mUi7HN6Zba9IEKaCmen6KvB93tvNxA8G2U4ga7LWnjym-bXbL__ozpwa7yzkpFiOamCXx-Fij5cW6d202qHobBjZDnaqnpeyaEYrqh9ID6kmstEF_LUGL3Btm1bQbZvUH7UDOo5Xz6u7_XjBdHGvDlAEDb0i5BsdUnGD2EVNpmbcjpP_J_ALldoMw</recordid><startdate>20171201</startdate><enddate>20171201</enddate><creator>Chang, Ming</creator><creator>Steinmetzer, Katrin</creator><creator>Raugi, Dana N.</creator><creator>Smith, Robert A.</creator><creator>Ba, Selly</creator><creator>Sall, Fatima</creator><creator>Seydi, Moussa</creator><creator>Niang, Alassane</creator><creator>Sall, ElHadji Ibrahima</creator><creator>Cisse, Ousseynou</creator><creator>Rödel, Katja</creator><creator>Coombs, Robert W.</creator><creator>Gottlieb, Geoffrey S.</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20171201</creationdate><title>Detection and differentiation of HIV-2 using the point-of-care Alere q HIV-1/2 Detect nucleic acid test</title><author>Chang, Ming ; Steinmetzer, Katrin ; Raugi, Dana N. ; Smith, Robert A. ; Ba, Selly ; Sall, Fatima ; Seydi, Moussa ; Niang, Alassane ; Sall, ElHadji Ibrahima ; Cisse, Ousseynou ; Rödel, Katja ; Coombs, Robert W. ; Gottlieb, Geoffrey S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-1e07299bd33884c3d4e2e38c8d754e584f91705dfb5a7aff967ab896d14e338c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Alere q HIV-1/2 Detect</topic><topic>Diagnosis</topic><topic>HIV-2</topic><topic>Nucleic acid test</topic><topic>Point-of-care</topic><topic>Qualitative</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chang, Ming</creatorcontrib><creatorcontrib>Steinmetzer, Katrin</creatorcontrib><creatorcontrib>Raugi, Dana N.</creatorcontrib><creatorcontrib>Smith, Robert A.</creatorcontrib><creatorcontrib>Ba, Selly</creatorcontrib><creatorcontrib>Sall, Fatima</creatorcontrib><creatorcontrib>Seydi, Moussa</creatorcontrib><creatorcontrib>Niang, Alassane</creatorcontrib><creatorcontrib>Sall, ElHadji Ibrahima</creatorcontrib><creatorcontrib>Cisse, Ousseynou</creatorcontrib><creatorcontrib>Rödel, Katja</creatorcontrib><creatorcontrib>Coombs, Robert W.</creatorcontrib><creatorcontrib>Gottlieb, Geoffrey S.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chang, Ming</au><au>Steinmetzer, Katrin</au><au>Raugi, Dana N.</au><au>Smith, Robert A.</au><au>Ba, Selly</au><au>Sall, Fatima</au><au>Seydi, Moussa</au><au>Niang, Alassane</au><au>Sall, ElHadji Ibrahima</au><au>Cisse, Ousseynou</au><au>Rödel, Katja</au><au>Coombs, Robert W.</au><au>Gottlieb, Geoffrey S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection and differentiation of HIV-2 using the point-of-care Alere q HIV-1/2 Detect nucleic acid test</atitle><jtitle>Journal of clinical virology</jtitle><addtitle>J Clin Virol</addtitle><date>2017-12-01</date><risdate>2017</risdate><volume>97</volume><spage>22</spage><epage>25</epage><pages>22-25</pages><issn>1386-6532</issn><eissn>1873-5967</eissn><abstract>•The Alere q HIV-1/2 Detect is a rapid point-of-care HIV nucleic acid test.•It can qualitatively detect and differentiate HIV-1 and HIV-2 in whole blood or plasma samples.
The Alere q HIV-1/2 Detect test (Alere Detect) is a rapid point-of-care (POC) nucleic acid test (NAT) that can detect and differentiate HIV-1 and HIV-2 in 25-μL whole blood or plasma samples. The Alere Detect test has been validated for early infant diagnosis of HIV-1 infection, and it is the only POC NAT device currently known to detect HIV-2, which is endemic in West Africa.
To evaluate the sensitivity detecting HIV-2 RNA and the differential performance of the Alere Detect.
Plasma samples from non-HIV (n=4), HIV-1 (n=22), HIV-2 (n=111; 29 Group A, 2 Group B) and HIV-1/HIV-2 dually-seropositive (n=8) participants in Senegal and the United States and HIV-2 reference strains (3 Group A, 1 Group B) were tested by Alere Detect, Abbott RealTime HIV-1 and the University of Washington HIV-2 RNA quantitative (UW HIV-2) assays.
The Alere Detect correctly differentiated between HIV-1 and HIV-2 in all 80 (100%) patient samples with detectable HIV RNA (n=20 HIV-1, 60 HIV-2). The overall HIV-2 detection concordance between Alere Detect and the UW HIV-2 assay was 68% (54/80); the concordance improved to 100% (30/30) for samples with HIV-2 RNA >300copies/mL. Neither assay detected HIV-2 RNA in 31 of 111 HIV-2 seropositive samples.
The Alere Detect test is a novel device detecting HIV RNA in clinical samples, and differentiating HIV-1 and HIV-2 with a high level of specificity. It has the potential for use as a rapid HIV-2 NAT-based diagnosis tool in resource-limited settings and to confirm HIV-2 infection for the CDC 4th generation HIV-1/2 diagnostic algorithm.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>29096389</pmid><doi>10.1016/j.jcv.2017.10.013</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Alere q HIV-1/2 Detect Diagnosis HIV-2 Nucleic acid test Point-of-care Qualitative |
| title | Detection and differentiation of HIV-2 using the point-of-care Alere q HIV-1/2 Detect nucleic acid test |
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