Insights into gene expression profiles induced by Socs3 depletion in keratinocytes
Specific deletion of suppressor of cytokine s ignaling 3 ( Socs3 ) in keratinocytes can cause severe skin inflammation with infiltration of immune cells. The molecular mechanisms and key regulatory pathways involved in these processes remain elusive. To investigate the role of Socs3 in keratinocytes...
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| Veröffentlicht in: | Scientific reports 2017-11, Vol.7 (1), p.15830-11, Article 15830 |
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| creator | Bajpai, Archana Ishii, Takashi Miyauchi, Kosuke Gupta, Vipul Nishio-Masaike, Yuka Shimizu-Yoshida, Yuki Kubo, Masato Kitano, Hiroaki |
| description | Specific deletion of suppressor of cytokine
s
ignaling 3 (
Socs3
) in keratinocytes can cause severe skin inflammation with infiltration of immune cells. The molecular mechanisms and key regulatory pathways involved in these processes remain elusive. To investigate the role of Socs3 in keratinocytes, we generated and analyzed global RNA-Seq profiles from
Socs3
conditional knockout (cKO) mice of two different ages (2 and 10 weeks). Over 400 genes were significantly regulated at both time points. Samples from 2-week-old mice exhibited down-regulation of genes involved in keratin-related functions and up-regulation of genes involved in lipid metabolism. At week 10, multiple chemokine and cytokine genes were up-regulated. Functional annotation revealed that the genes differentially expressed in the 2-week-old mice play roles in keratinization, keratinocyte differentiation, and epidermal cell differentiation. By contrast, differentially expressed genes in the 10-week-old animals are involved in acute immune-related functions. A group of activator protein-1–related genes were highly up-regulated in
Socs3
cKO mice of both ages. This observation was validated using qRT-PCR by SOCS3-depleted human keratinocyte–derived HaCaT cells. Our results suggest that, in addition to participating in immune-mediated pathways, SOCS3 also plays important roles in skin barrier homeostasis. |
| doi_str_mv | 10.1038/s41598-017-16155-1 |
| format | Article |
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s
ignaling 3 (
Socs3
) in keratinocytes can cause severe skin inflammation with infiltration of immune cells. The molecular mechanisms and key regulatory pathways involved in these processes remain elusive. To investigate the role of Socs3 in keratinocytes, we generated and analyzed global RNA-Seq profiles from
Socs3
conditional knockout (cKO) mice of two different ages (2 and 10 weeks). Over 400 genes were significantly regulated at both time points. Samples from 2-week-old mice exhibited down-regulation of genes involved in keratin-related functions and up-regulation of genes involved in lipid metabolism. At week 10, multiple chemokine and cytokine genes were up-regulated. Functional annotation revealed that the genes differentially expressed in the 2-week-old mice play roles in keratinization, keratinocyte differentiation, and epidermal cell differentiation. By contrast, differentially expressed genes in the 10-week-old animals are involved in acute immune-related functions. A group of activator protein-1–related genes were highly up-regulated in
Socs3
cKO mice of both ages. This observation was validated using qRT-PCR by SOCS3-depleted human keratinocyte–derived HaCaT cells. Our results suggest that, in addition to participating in immune-mediated pathways, SOCS3 also plays important roles in skin barrier homeostasis.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-017-16155-1</identifier><identifier>PMID: 29158586</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>38/39 ; 38/91 ; 631/114 ; 631/114/2785 ; 631/553 ; 64/60 ; Activator protein 1 ; Cell differentiation ; Clonal deletion ; Cytokines ; Gene deletion ; Gene expression ; Gene regulation ; Homeostasis ; Humanities and Social Sciences ; Keratin ; Keratinization ; Keratinocytes ; Lipid metabolism ; Molecular modelling ; multidisciplinary ; Ribonucleic acid ; RNA ; Science ; Science (multidisciplinary) ; Skin ; SOCS-3 protein</subject><ispartof>Scientific reports, 2017-11, Vol.7 (1), p.15830-11, Article 15830</ispartof><rights>The Author(s) 2017</rights><rights>2017. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c518t-b70fcd0ac5e437062e712baf6974b8e4ea555a8af79515311dd548898d9a9e353</citedby><cites>FETCH-LOGICAL-c518t-b70fcd0ac5e437062e712baf6974b8e4ea555a8af79515311dd548898d9a9e353</cites><orcidid>0000-0003-3345-7288</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5696538/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5696538/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29158586$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bajpai, Archana</creatorcontrib><creatorcontrib>Ishii, Takashi</creatorcontrib><creatorcontrib>Miyauchi, Kosuke</creatorcontrib><creatorcontrib>Gupta, Vipul</creatorcontrib><creatorcontrib>Nishio-Masaike, Yuka</creatorcontrib><creatorcontrib>Shimizu-Yoshida, Yuki</creatorcontrib><creatorcontrib>Kubo, Masato</creatorcontrib><creatorcontrib>Kitano, Hiroaki</creatorcontrib><title>Insights into gene expression profiles induced by Socs3 depletion in keratinocytes</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Specific deletion of suppressor of cytokine
s
ignaling 3 (
Socs3
) in keratinocytes can cause severe skin inflammation with infiltration of immune cells. The molecular mechanisms and key regulatory pathways involved in these processes remain elusive. To investigate the role of Socs3 in keratinocytes, we generated and analyzed global RNA-Seq profiles from
Socs3
conditional knockout (cKO) mice of two different ages (2 and 10 weeks). Over 400 genes were significantly regulated at both time points. Samples from 2-week-old mice exhibited down-regulation of genes involved in keratin-related functions and up-regulation of genes involved in lipid metabolism. At week 10, multiple chemokine and cytokine genes were up-regulated. Functional annotation revealed that the genes differentially expressed in the 2-week-old mice play roles in keratinization, keratinocyte differentiation, and epidermal cell differentiation. By contrast, differentially expressed genes in the 10-week-old animals are involved in acute immune-related functions. A group of activator protein-1–related genes were highly up-regulated in
Socs3
cKO mice of both ages. This observation was validated using qRT-PCR by SOCS3-depleted human keratinocyte–derived HaCaT cells. Our results suggest that, in addition to participating in immune-mediated pathways, SOCS3 also plays important roles in skin barrier homeostasis.</description><subject>38/39</subject><subject>38/91</subject><subject>631/114</subject><subject>631/114/2785</subject><subject>631/553</subject><subject>64/60</subject><subject>Activator protein 1</subject><subject>Cell differentiation</subject><subject>Clonal deletion</subject><subject>Cytokines</subject><subject>Gene deletion</subject><subject>Gene expression</subject><subject>Gene regulation</subject><subject>Homeostasis</subject><subject>Humanities and Social Sciences</subject><subject>Keratin</subject><subject>Keratinization</subject><subject>Keratinocytes</subject><subject>Lipid metabolism</subject><subject>Molecular modelling</subject><subject>multidisciplinary</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Skin</subject><subject>SOCS-3 protein</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kV1LHDEUhoNYVLb-gV6UAW-8mTYfc2aSG6GIVUEoqL0OmcyZNXY2mSYz4v77Zl0r24K5SeA85z05PIR8YvQLo0J-TRUDJUvKmpLVDKBke-SI0wpKLjjf33kfkuOUHmk-wFXF1AE55IqBBFkfkdtrn9zyYUqF81MoluixwOcxYkou-GKMoXcDbqrdbLEr2nVxF2wSRYfjgNOGcb74hdFMzge7njB9JB96MyQ8fr0X5Of3i_vzq_Lmx-X1-beb0gKTU9k2tLcdNRawEg2tOTaMt6avVVO1Eis0AGCk6RsFDARjXQeVlEp2yigUIBbkbJs7zu0KO4t-imbQY3QrE9c6GKf_rXj3oJfhSUOtahAyB5y-BsTwe8Y06ZVLFofBeAxz0kzVtVIKQGT05D_0MczR5_VeqIpKka0sCN9SNoaUIvZvn2FUb6zprTWdrekXa5rlps-7a7y1_HWUAbEFUi75Jcad2e_H_gH8_aN9</recordid><startdate>20171120</startdate><enddate>20171120</enddate><creator>Bajpai, Archana</creator><creator>Ishii, Takashi</creator><creator>Miyauchi, Kosuke</creator><creator>Gupta, Vipul</creator><creator>Nishio-Masaike, Yuka</creator><creator>Shimizu-Yoshida, Yuki</creator><creator>Kubo, Masato</creator><creator>Kitano, Hiroaki</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-3345-7288</orcidid></search><sort><creationdate>20171120</creationdate><title>Insights into gene expression profiles induced by Socs3 depletion in keratinocytes</title><author>Bajpai, Archana ; 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s
ignaling 3 (
Socs3
) in keratinocytes can cause severe skin inflammation with infiltration of immune cells. The molecular mechanisms and key regulatory pathways involved in these processes remain elusive. To investigate the role of Socs3 in keratinocytes, we generated and analyzed global RNA-Seq profiles from
Socs3
conditional knockout (cKO) mice of two different ages (2 and 10 weeks). Over 400 genes were significantly regulated at both time points. Samples from 2-week-old mice exhibited down-regulation of genes involved in keratin-related functions and up-regulation of genes involved in lipid metabolism. At week 10, multiple chemokine and cytokine genes were up-regulated. Functional annotation revealed that the genes differentially expressed in the 2-week-old mice play roles in keratinization, keratinocyte differentiation, and epidermal cell differentiation. By contrast, differentially expressed genes in the 10-week-old animals are involved in acute immune-related functions. A group of activator protein-1–related genes were highly up-regulated in
Socs3
cKO mice of both ages. This observation was validated using qRT-PCR by SOCS3-depleted human keratinocyte–derived HaCaT cells. Our results suggest that, in addition to participating in immune-mediated pathways, SOCS3 also plays important roles in skin barrier homeostasis.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>29158586</pmid><doi>10.1038/s41598-017-16155-1</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-3345-7288</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 38/39 38/91 631/114 631/114/2785 631/553 64/60 Activator protein 1 Cell differentiation Clonal deletion Cytokines Gene deletion Gene expression Gene regulation Homeostasis Humanities and Social Sciences Keratin Keratinization Keratinocytes Lipid metabolism Molecular modelling multidisciplinary Ribonucleic acid RNA Science Science (multidisciplinary) Skin SOCS-3 protein |
| title | Insights into gene expression profiles induced by Socs3 depletion in keratinocytes |
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