Evolutionary structure of Plasmodium falciparum major variant surface antigen genes in South America: Implications for epidemic transmission and surveillance
Strong founder effects resulting from human migration out of Africa have led to geographic variation in single nucleotide polymorphisms (SNPs) and microsatellites (MS) of the malaria parasite, Plasmodium falciparum. This is particularly striking in South America where two major founder populations o...
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| Veröffentlicht in: | BMC evolutionary biology 2017-11, Vol.7 (22), p.9376-9390 |
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| creator | Rougeron, Virginie Tiedje, Kathryn E. Chen, Donald S. Rask, Thomas S. Gamboa, Dionicia Maestre, Amanda Musset, Lise Legrand, Eric Noya, Oscar Yalcindag, Erhan Renaud, François Prugnolle, Franck Day, Karen P. |
| description | Strong founder effects resulting from human migration out of Africa have led to geographic variation in single nucleotide polymorphisms (SNPs) and microsatellites (MS) of the malaria parasite, Plasmodium falciparum. This is particularly striking in South America where two major founder populations of P. falciparum have been identified that are presumed to have arisen from the transatlantic slave trade. Given the importance of the major variant surface antigen of the blood stages of P. falciparum as both a virulence factor and target of immunity, we decided to investigate the population genetics of the genes encoding “Plasmodium falciparum Erythrocyte Membrane Protein 1” (PfEMP1) among several countries in South America, in order to evaluate the transmission patterns of malaria in this continent. Deep sequencing of the DBLα domain of var genes from 128 P. falciparum isolates from five locations in South America was completed using a 454 high throughput sequencing protocol. Striking geographic variation in var DBLα sequences, similar to that seen for SNPs and MS markers, was observed. Colombia and French Guiana had distinct var DBLα sequences, whereas Peru and Venezuela showed an admixture. The importance of such geographic variation to herd immunity and malaria vaccination is discussed.
Given the importance of the major variant surface antigen of the blood stages of Plasmodium falciparum as both a virulence factor and target of immunity, we decided to investigate the evolutionary patterns of the genes encoding “Plasmodium falciparum Erythrocyte Membrane Protein 1” (PfEMP1), in several countries of South America, in order to evaluate the transmission patterns of malaria in this continent. Deep sequencing of the DBLα domain of var genes from 128 P. falciparum isolates from five locations in South America was completed using 454 sequencing. Striking geographic variation in var DBLα types, surprisingly similar to that seen for microsatellite and SNP markers, was observed. Colombia and French Guiana had distinct var DBLα types, whereas Peru and Venezuela showed an admixture. The importance of such geographic variation to herd immunity and malaria vaccination is discussed. |
| doi_str_mv | 10.1002/ece3.3425 |
| format | Article |
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Given the importance of the major variant surface antigen of the blood stages of Plasmodium falciparum as both a virulence factor and target of immunity, we decided to investigate the evolutionary patterns of the genes encoding “Plasmodium falciparum Erythrocyte Membrane Protein 1” (PfEMP1), in several countries of South America, in order to evaluate the transmission patterns of malaria in this continent. Deep sequencing of the DBLα domain of var genes from 128 P. falciparum isolates from five locations in South America was completed using 454 sequencing. Striking geographic variation in var DBLα types, surprisingly similar to that seen for microsatellite and SNP markers, was observed. Colombia and French Guiana had distinct var DBLα types, whereas Peru and Venezuela showed an admixture. The importance of such geographic variation to herd immunity and malaria vaccination is discussed.</description><identifier>ISSN: 2045-7758</identifier><identifier>ISSN: 1471-2148</identifier><identifier>EISSN: 2045-7758</identifier><identifier>EISSN: 1471-2148</identifier><identifier>DOI: 10.1002/ece3.3425</identifier><identifier>PMID: 29187975</identifier><language>eng</language><publisher>England: John Wiley & Sons, Inc</publisher><subject>Antigens ; Biological evolution ; Epidemics ; Erythrocyte membrane protein 1 ; Erythrocytes ; evolutionary structure ; Gene sequencing ; Genes ; Genetics ; Herd immunity ; Immunity ; International trade ; Life Sciences ; Malaria ; Membrane proteins ; Microbiology and Parasitology ; Microsatellites ; Migration ; Next-generation sequencing ; Original Research ; Parasitology ; Plasmodium falciparum ; Plasmodium falciparum Erythrocyte Membrane Protein 1 ; Population genetics ; population genomics ; Single-nucleotide polymorphism ; Vaccination ; var genes ; Vector-borne diseases ; Virulence ; Virulence factors</subject><ispartof>BMC evolutionary biology, 2017-11, Vol.7 (22), p.9376-9390</ispartof><rights>2017 The Authors. published by John Wiley & Sons Ltd.</rights><rights>2017. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). 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This is particularly striking in South America where two major founder populations of P. falciparum have been identified that are presumed to have arisen from the transatlantic slave trade. Given the importance of the major variant surface antigen of the blood stages of P. falciparum as both a virulence factor and target of immunity, we decided to investigate the population genetics of the genes encoding “Plasmodium falciparum Erythrocyte Membrane Protein 1” (PfEMP1) among several countries in South America, in order to evaluate the transmission patterns of malaria in this continent. Deep sequencing of the DBLα domain of var genes from 128 P. falciparum isolates from five locations in South America was completed using a 454 high throughput sequencing protocol. Striking geographic variation in var DBLα sequences, similar to that seen for SNPs and MS markers, was observed. Colombia and French Guiana had distinct var DBLα sequences, whereas Peru and Venezuela showed an admixture. The importance of such geographic variation to herd immunity and malaria vaccination is discussed.
Given the importance of the major variant surface antigen of the blood stages of Plasmodium falciparum as both a virulence factor and target of immunity, we decided to investigate the evolutionary patterns of the genes encoding “Plasmodium falciparum Erythrocyte Membrane Protein 1” (PfEMP1), in several countries of South America, in order to evaluate the transmission patterns of malaria in this continent. Deep sequencing of the DBLα domain of var genes from 128 P. falciparum isolates from five locations in South America was completed using 454 sequencing. Striking geographic variation in var DBLα types, surprisingly similar to that seen for microsatellite and SNP markers, was observed. Colombia and French Guiana had distinct var DBLα types, whereas Peru and Venezuela showed an admixture. The importance of such geographic variation to herd immunity and malaria vaccination is discussed.</description><subject>Antigens</subject><subject>Biological evolution</subject><subject>Epidemics</subject><subject>Erythrocyte membrane protein 1</subject><subject>Erythrocytes</subject><subject>evolutionary structure</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genetics</subject><subject>Herd immunity</subject><subject>Immunity</subject><subject>International trade</subject><subject>Life Sciences</subject><subject>Malaria</subject><subject>Membrane proteins</subject><subject>Microbiology and Parasitology</subject><subject>Microsatellites</subject><subject>Migration</subject><subject>Next-generation sequencing</subject><subject>Original Research</subject><subject>Parasitology</subject><subject>Plasmodium falciparum</subject><subject>Plasmodium falciparum Erythrocyte Membrane Protein 1</subject><subject>Population genetics</subject><subject>population genomics</subject><subject>Single-nucleotide polymorphism</subject><subject>Vaccination</subject><subject>var genes</subject><subject>Vector-borne diseases</subject><subject>Virulence</subject><subject>Virulence 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structure of Plasmodium falciparum major variant surface antigen genes in South America: Implications for epidemic transmission and surveillance</title><author>Rougeron, Virginie ; Tiedje, Kathryn E. ; Chen, Donald S. ; Rask, Thomas S. ; Gamboa, Dionicia ; Maestre, Amanda ; Musset, Lise ; Legrand, Eric ; Noya, Oscar ; Yalcindag, Erhan ; Renaud, François ; Prugnolle, Franck ; Day, Karen P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4815-4070c71d648d0273d09d58ad3212493b74787064ea7e9aaa4c932a72c9d1d3fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Antigens</topic><topic>Biological evolution</topic><topic>Epidemics</topic><topic>Erythrocyte membrane protein 1</topic><topic>Erythrocytes</topic><topic>evolutionary structure</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Genetics</topic><topic>Herd immunity</topic><topic>Immunity</topic><topic>International trade</topic><topic>Life Sciences</topic><topic>Malaria</topic><topic>Membrane proteins</topic><topic>Microbiology and Parasitology</topic><topic>Microsatellites</topic><topic>Migration</topic><topic>Next-generation sequencing</topic><topic>Original Research</topic><topic>Parasitology</topic><topic>Plasmodium falciparum</topic><topic>Plasmodium falciparum Erythrocyte Membrane Protein 1</topic><topic>Population genetics</topic><topic>population genomics</topic><topic>Single-nucleotide polymorphism</topic><topic>Vaccination</topic><topic>var genes</topic><topic>Vector-borne diseases</topic><topic>Virulence</topic><topic>Virulence factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rougeron, Virginie</creatorcontrib><creatorcontrib>Tiedje, Kathryn E.</creatorcontrib><creatorcontrib>Chen, Donald S.</creatorcontrib><creatorcontrib>Rask, Thomas 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Kathryn E.</au><au>Chen, Donald S.</au><au>Rask, Thomas S.</au><au>Gamboa, Dionicia</au><au>Maestre, Amanda</au><au>Musset, Lise</au><au>Legrand, Eric</au><au>Noya, Oscar</au><au>Yalcindag, Erhan</au><au>Renaud, François</au><au>Prugnolle, Franck</au><au>Day, Karen P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evolutionary structure of Plasmodium falciparum major variant surface antigen genes in South America: Implications for epidemic transmission and surveillance</atitle><jtitle>BMC evolutionary biology</jtitle><addtitle>Ecol Evol</addtitle><date>2017-11</date><risdate>2017</risdate><volume>7</volume><issue>22</issue><spage>9376</spage><epage>9390</epage><pages>9376-9390</pages><issn>2045-7758</issn><issn>1471-2148</issn><eissn>2045-7758</eissn><eissn>1471-2148</eissn><abstract>Strong founder effects resulting from human migration out of Africa have led to geographic variation in single nucleotide polymorphisms (SNPs) and microsatellites (MS) of the malaria parasite, Plasmodium falciparum. This is particularly striking in South America where two major founder populations of P. falciparum have been identified that are presumed to have arisen from the transatlantic slave trade. Given the importance of the major variant surface antigen of the blood stages of P. falciparum as both a virulence factor and target of immunity, we decided to investigate the population genetics of the genes encoding “Plasmodium falciparum Erythrocyte Membrane Protein 1” (PfEMP1) among several countries in South America, in order to evaluate the transmission patterns of malaria in this continent. Deep sequencing of the DBLα domain of var genes from 128 P. falciparum isolates from five locations in South America was completed using a 454 high throughput sequencing protocol. Striking geographic variation in var DBLα sequences, similar to that seen for SNPs and MS markers, was observed. Colombia and French Guiana had distinct var DBLα sequences, whereas Peru and Venezuela showed an admixture. The importance of such geographic variation to herd immunity and malaria vaccination is discussed.
Given the importance of the major variant surface antigen of the blood stages of Plasmodium falciparum as both a virulence factor and target of immunity, we decided to investigate the evolutionary patterns of the genes encoding “Plasmodium falciparum Erythrocyte Membrane Protein 1” (PfEMP1), in several countries of South America, in order to evaluate the transmission patterns of malaria in this continent. Deep sequencing of the DBLα domain of var genes from 128 P. falciparum isolates from five locations in South America was completed using 454 sequencing. Striking geographic variation in var DBLα types, surprisingly similar to that seen for microsatellite and SNP markers, was observed. Colombia and French Guiana had distinct var DBLα types, whereas Peru and Venezuela showed an admixture. The importance of such geographic variation to herd immunity and malaria vaccination is discussed.</abstract><cop>England</cop><pub>John Wiley & Sons, Inc</pub><pmid>29187975</pmid><doi>10.1002/ece3.3425</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0001-5873-5681</orcidid><orcidid>https://orcid.org/0000-0003-0215-4110</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Wiley-Blackwell Journals; DOAJ Directory of Open Access Journals; PubMed Central Open Access; Wiley Online Library Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Antigens Biological evolution Epidemics Erythrocyte membrane protein 1 Erythrocytes evolutionary structure Gene sequencing Genes Genetics Herd immunity Immunity International trade Life Sciences Malaria Membrane proteins Microbiology and Parasitology Microsatellites Migration Next-generation sequencing Original Research Parasitology Plasmodium falciparum Plasmodium falciparum Erythrocyte Membrane Protein 1 Population genetics population genomics Single-nucleotide polymorphism Vaccination var genes Vector-borne diseases Virulence Virulence factors |
| title | Evolutionary structure of Plasmodium falciparum major variant surface antigen genes in South America: Implications for epidemic transmission and surveillance |
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