Successful amplification of DNA aboard the International Space Station
As the range and duration of human ventures into space increase, it becomes imperative that we understand the effects of the cosmic environment on astronaut health. Molecular technologies now widely used in research and medicine will need to become available in space to ensure appropriate care of as...
Gespeichert in:
| Veröffentlicht in: | NPJ microgravity 2017-11, Vol.3 (1), p.26-4, Article 26 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 4 |
|---|---|
| container_issue | 1 |
| container_start_page | 26 |
| container_title | NPJ microgravity |
| container_volume | 3 |
| creator | Boguraev, Anna-Sophia Christensen, Holly C. Bonneau, Ashley R. Pezza, John A. Nichols, Nicole M. Giraldez, Antonio J. Gray, Michelle M. Wagner, Brandon M. Aken, Jordan T. Foley, Kevin D. Copeland, D. Scott Kraves, Sebastian Alvarez Saavedra, Ezequiel |
| description | As the range and duration of human ventures into space increase, it becomes imperative that we understand the effects of the cosmic environment on astronaut health. Molecular technologies now widely used in research and medicine will need to become available in space to ensure appropriate care of astronauts. The polymerase chain reaction (PCR) is the gold standard for DNA analysis, yet its potential for use on-orbit remains under-explored. We describe DNA amplification aboard the International Space Station (ISS) through the use of a miniaturized miniPCR system. Target sequences in plasmid, zebrafish genomic DNA, and bisulfite-treated DNA were successfully amplified under a variety of conditions. Methylation-specific primers differentially amplified bisulfite-treated samples as would be expected under standard laboratory conditions. Our findings establish proof of concept for targeted detection of DNA sequences during spaceflight and lay a foundation for future uses ranging from environmental monitoring to on-orbit diagnostics. |
| doi_str_mv | 10.1038/s41526-017-0033-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5691047</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1967863806</sourcerecordid><originalsourceid>FETCH-LOGICAL-c470t-cb058cda21d10069c0b0f570b945d084d8a56f64b22cefd1ab3b468561960dec3</originalsourceid><addsrcrecordid>eNp1kcFLwzAUxoMobsz9AV6k4MVL9aVp0uYijOl0MPQwPYc0TbeOrplJK_jfm61zTMFTEr7f-957-RC6xHCLgaR3LsY0YiHgJAQgJOQnqB-RhIQpMHp6dO-hoXMrAMAxSzmNzlEv4pglKeZ9NJm3SmnnirYK5HpTlUWpZFOaOjBF8PAyCmRmpM2DZqmDad1oW-9UWQXzjVQ6mDe79wU6K2Tl9HB_DtD75PFt_BzOXp-m49EsVHECTagyoKnKZYRzDMC4ggwKmkDGY5pDGueppKxgcRZFShc5lhnJ_NCUYc4g14oM0H3nu2mztc6VrhsrK7Gx5VraL2FkKX4rdbkUC_MpKOMY4sQb3OwNrPlotWvEunRKV5WstWmd8I2SlBH_bx69_oOuTOv3r3YUxZTEjHgKd5Syxjmri8MwGMQ2KNEFJXxQYhuU4L7m6niLQ8VPLB6IOsB5qV5oe9T6X9dvb1Wdug</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1965153463</pqid></control><display><type>article</type><title>Successful amplification of DNA aboard the International Space Station</title><source>DOAJ Directory of Open Access Journals</source><source>Springer Nature OA Free Journals</source><source>Nature Free</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Boguraev, Anna-Sophia ; Christensen, Holly C. ; Bonneau, Ashley R. ; Pezza, John A. ; Nichols, Nicole M. ; Giraldez, Antonio J. ; Gray, Michelle M. ; Wagner, Brandon M. ; Aken, Jordan T. ; Foley, Kevin D. ; Copeland, D. Scott ; Kraves, Sebastian ; Alvarez Saavedra, Ezequiel</creator><creatorcontrib>Boguraev, Anna-Sophia ; Christensen, Holly C. ; Bonneau, Ashley R. ; Pezza, John A. ; Nichols, Nicole M. ; Giraldez, Antonio J. ; Gray, Michelle M. ; Wagner, Brandon M. ; Aken, Jordan T. ; Foley, Kevin D. ; Copeland, D. Scott ; Kraves, Sebastian ; Alvarez Saavedra, Ezequiel</creatorcontrib><description>As the range and duration of human ventures into space increase, it becomes imperative that we understand the effects of the cosmic environment on astronaut health. Molecular technologies now widely used in research and medicine will need to become available in space to ensure appropriate care of astronauts. The polymerase chain reaction (PCR) is the gold standard for DNA analysis, yet its potential for use on-orbit remains under-explored. We describe DNA amplification aboard the International Space Station (ISS) through the use of a miniaturized miniPCR system. Target sequences in plasmid, zebrafish genomic DNA, and bisulfite-treated DNA were successfully amplified under a variety of conditions. Methylation-specific primers differentially amplified bisulfite-treated samples as would be expected under standard laboratory conditions. Our findings establish proof of concept for targeted detection of DNA sequences during spaceflight and lay a foundation for future uses ranging from environmental monitoring to on-orbit diagnostics.</description><identifier>ISSN: 2373-8065</identifier><identifier>EISSN: 2373-8065</identifier><identifier>DOI: 10.1038/s41526-017-0033-9</identifier><identifier>PMID: 29167819</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/1647 ; 631/337 ; Applied Microbiology ; Biomedical and Life Sciences ; Biotechnology ; Bisulfite ; Brief Communication ; Classical and Continuum Physics ; Deoxyribonucleic acid ; DNA ; DNA methylation ; Environmental monitoring ; Immunology ; Life Sciences ; Multinational space ventures ; Nucleotide sequence ; Polymerase chain reaction ; Primers ; Space Exploration and Astronautics ; Space flight ; Space Sciences (including Extraterrestrial Physics ; Space stations</subject><ispartof>NPJ microgravity, 2017-11, Vol.3 (1), p.26-4, Article 26</ispartof><rights>The Author(s) 2017</rights><rights>2017. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-cb058cda21d10069c0b0f570b945d084d8a56f64b22cefd1ab3b468561960dec3</citedby><cites>FETCH-LOGICAL-c470t-cb058cda21d10069c0b0f570b945d084d8a56f64b22cefd1ab3b468561960dec3</cites><orcidid>0000-0001-9148-0879</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5691047/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5691047/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29167819$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Boguraev, Anna-Sophia</creatorcontrib><creatorcontrib>Christensen, Holly C.</creatorcontrib><creatorcontrib>Bonneau, Ashley R.</creatorcontrib><creatorcontrib>Pezza, John A.</creatorcontrib><creatorcontrib>Nichols, Nicole M.</creatorcontrib><creatorcontrib>Giraldez, Antonio J.</creatorcontrib><creatorcontrib>Gray, Michelle M.</creatorcontrib><creatorcontrib>Wagner, Brandon M.</creatorcontrib><creatorcontrib>Aken, Jordan T.</creatorcontrib><creatorcontrib>Foley, Kevin D.</creatorcontrib><creatorcontrib>Copeland, D. Scott</creatorcontrib><creatorcontrib>Kraves, Sebastian</creatorcontrib><creatorcontrib>Alvarez Saavedra, Ezequiel</creatorcontrib><title>Successful amplification of DNA aboard the International Space Station</title><title>NPJ microgravity</title><addtitle>npj Microgravity</addtitle><addtitle>NPJ Microgravity</addtitle><description>As the range and duration of human ventures into space increase, it becomes imperative that we understand the effects of the cosmic environment on astronaut health. Molecular technologies now widely used in research and medicine will need to become available in space to ensure appropriate care of astronauts. The polymerase chain reaction (PCR) is the gold standard for DNA analysis, yet its potential for use on-orbit remains under-explored. We describe DNA amplification aboard the International Space Station (ISS) through the use of a miniaturized miniPCR system. Target sequences in plasmid, zebrafish genomic DNA, and bisulfite-treated DNA were successfully amplified under a variety of conditions. Methylation-specific primers differentially amplified bisulfite-treated samples as would be expected under standard laboratory conditions. Our findings establish proof of concept for targeted detection of DNA sequences during spaceflight and lay a foundation for future uses ranging from environmental monitoring to on-orbit diagnostics.</description><subject>631/1647</subject><subject>631/337</subject><subject>Applied Microbiology</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Bisulfite</subject><subject>Brief Communication</subject><subject>Classical and Continuum Physics</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA methylation</subject><subject>Environmental monitoring</subject><subject>Immunology</subject><subject>Life Sciences</subject><subject>Multinational space ventures</subject><subject>Nucleotide sequence</subject><subject>Polymerase chain reaction</subject><subject>Primers</subject><subject>Space Exploration and Astronautics</subject><subject>Space flight</subject><subject>Space Sciences (including Extraterrestrial Physics</subject><subject>Space stations</subject><issn>2373-8065</issn><issn>2373-8065</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kcFLwzAUxoMobsz9AV6k4MVL9aVp0uYijOl0MPQwPYc0TbeOrplJK_jfm61zTMFTEr7f-957-RC6xHCLgaR3LsY0YiHgJAQgJOQnqB-RhIQpMHp6dO-hoXMrAMAxSzmNzlEv4pglKeZ9NJm3SmnnirYK5HpTlUWpZFOaOjBF8PAyCmRmpM2DZqmDad1oW-9UWQXzjVQ6mDe79wU6K2Tl9HB_DtD75PFt_BzOXp-m49EsVHECTagyoKnKZYRzDMC4ggwKmkDGY5pDGueppKxgcRZFShc5lhnJ_NCUYc4g14oM0H3nu2mztc6VrhsrK7Gx5VraL2FkKX4rdbkUC_MpKOMY4sQb3OwNrPlotWvEunRKV5WstWmd8I2SlBH_bx69_oOuTOv3r3YUxZTEjHgKd5Syxjmri8MwGMQ2KNEFJXxQYhuU4L7m6niLQ8VPLB6IOsB5qV5oe9T6X9dvb1Wdug</recordid><startdate>20171116</startdate><enddate>20171116</enddate><creator>Boguraev, Anna-Sophia</creator><creator>Christensen, Holly C.</creator><creator>Bonneau, Ashley R.</creator><creator>Pezza, John A.</creator><creator>Nichols, Nicole M.</creator><creator>Giraldez, Antonio J.</creator><creator>Gray, Michelle M.</creator><creator>Wagner, Brandon M.</creator><creator>Aken, Jordan T.</creator><creator>Foley, Kevin D.</creator><creator>Copeland, D. Scott</creator><creator>Kraves, Sebastian</creator><creator>Alvarez Saavedra, Ezequiel</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-9148-0879</orcidid></search><sort><creationdate>20171116</creationdate><title>Successful amplification of DNA aboard the International Space Station</title><author>Boguraev, Anna-Sophia ; Christensen, Holly C. ; Bonneau, Ashley R. ; Pezza, John A. ; Nichols, Nicole M. ; Giraldez, Antonio J. ; Gray, Michelle M. ; Wagner, Brandon M. ; Aken, Jordan T. ; Foley, Kevin D. ; Copeland, D. Scott ; Kraves, Sebastian ; Alvarez Saavedra, Ezequiel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-cb058cda21d10069c0b0f570b945d084d8a56f64b22cefd1ab3b468561960dec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>631/1647</topic><topic>631/337</topic><topic>Applied Microbiology</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Bisulfite</topic><topic>Brief Communication</topic><topic>Classical and Continuum Physics</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA methylation</topic><topic>Environmental monitoring</topic><topic>Immunology</topic><topic>Life Sciences</topic><topic>Multinational space ventures</topic><topic>Nucleotide sequence</topic><topic>Polymerase chain reaction</topic><topic>Primers</topic><topic>Space Exploration and Astronautics</topic><topic>Space flight</topic><topic>Space Sciences (including Extraterrestrial Physics</topic><topic>Space stations</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boguraev, Anna-Sophia</creatorcontrib><creatorcontrib>Christensen, Holly C.</creatorcontrib><creatorcontrib>Bonneau, Ashley R.</creatorcontrib><creatorcontrib>Pezza, John A.</creatorcontrib><creatorcontrib>Nichols, Nicole M.</creatorcontrib><creatorcontrib>Giraldez, Antonio J.</creatorcontrib><creatorcontrib>Gray, Michelle M.</creatorcontrib><creatorcontrib>Wagner, Brandon M.</creatorcontrib><creatorcontrib>Aken, Jordan T.</creatorcontrib><creatorcontrib>Foley, Kevin D.</creatorcontrib><creatorcontrib>Copeland, D. Scott</creatorcontrib><creatorcontrib>Kraves, Sebastian</creatorcontrib><creatorcontrib>Alvarez Saavedra, Ezequiel</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>NPJ microgravity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boguraev, Anna-Sophia</au><au>Christensen, Holly C.</au><au>Bonneau, Ashley R.</au><au>Pezza, John A.</au><au>Nichols, Nicole M.</au><au>Giraldez, Antonio J.</au><au>Gray, Michelle M.</au><au>Wagner, Brandon M.</au><au>Aken, Jordan T.</au><au>Foley, Kevin D.</au><au>Copeland, D. Scott</au><au>Kraves, Sebastian</au><au>Alvarez Saavedra, Ezequiel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Successful amplification of DNA aboard the International Space Station</atitle><jtitle>NPJ microgravity</jtitle><stitle>npj Microgravity</stitle><addtitle>NPJ Microgravity</addtitle><date>2017-11-16</date><risdate>2017</risdate><volume>3</volume><issue>1</issue><spage>26</spage><epage>4</epage><pages>26-4</pages><artnum>26</artnum><issn>2373-8065</issn><eissn>2373-8065</eissn><abstract>As the range and duration of human ventures into space increase, it becomes imperative that we understand the effects of the cosmic environment on astronaut health. Molecular technologies now widely used in research and medicine will need to become available in space to ensure appropriate care of astronauts. The polymerase chain reaction (PCR) is the gold standard for DNA analysis, yet its potential for use on-orbit remains under-explored. We describe DNA amplification aboard the International Space Station (ISS) through the use of a miniaturized miniPCR system. Target sequences in plasmid, zebrafish genomic DNA, and bisulfite-treated DNA were successfully amplified under a variety of conditions. Methylation-specific primers differentially amplified bisulfite-treated samples as would be expected under standard laboratory conditions. Our findings establish proof of concept for targeted detection of DNA sequences during spaceflight and lay a foundation for future uses ranging from environmental monitoring to on-orbit diagnostics.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>29167819</pmid><doi>10.1038/s41526-017-0033-9</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0001-9148-0879</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2373-8065 |
| ispartof | NPJ microgravity, 2017-11, Vol.3 (1), p.26-4, Article 26 |
| issn | 2373-8065 2373-8065 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5691047 |
| source | DOAJ Directory of Open Access Journals; Springer Nature OA Free Journals; Nature Free; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | 631/1647 631/337 Applied Microbiology Biomedical and Life Sciences Biotechnology Bisulfite Brief Communication Classical and Continuum Physics Deoxyribonucleic acid DNA DNA methylation Environmental monitoring Immunology Life Sciences Multinational space ventures Nucleotide sequence Polymerase chain reaction Primers Space Exploration and Astronautics Space flight Space Sciences (including Extraterrestrial Physics Space stations |
| title | Successful amplification of DNA aboard the International Space Station |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T18%3A20%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Successful%20amplification%20of%20DNA%20aboard%20the%20International%20Space%20Station&rft.jtitle=NPJ%20microgravity&rft.au=Boguraev,%20Anna-Sophia&rft.date=2017-11-16&rft.volume=3&rft.issue=1&rft.spage=26&rft.epage=4&rft.pages=26-4&rft.artnum=26&rft.issn=2373-8065&rft.eissn=2373-8065&rft_id=info:doi/10.1038/s41526-017-0033-9&rft_dat=%3Cproquest_pubme%3E1967863806%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1965153463&rft_id=info:pmid/29167819&rfr_iscdi=true |