Bone Marrow, Adipose, and Lung Tissue‐Derived Murine Mesenchymal Stromal Cells Release Different Mediators and Differentially Affect Airway and Lung Parenchyma in Experimental Asthma
Mesenchymal stromal cells (MSCs) from different sources have differential effects on lung injury. To compare the effects of murine MSCs from bone marrow (BM), adipose tissue (AD), and lung tissue (LUNG) on inflammatory and remodeling processes in experimental allergic asthma, female C57BL/6 mice wer...
Gespeichert in:
| Veröffentlicht in: | Stem cells translational medicine 2017-06, Vol.6 (6), p.1557-1567 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1567 |
|---|---|
| container_issue | 6 |
| container_start_page | 1557 |
| container_title | Stem cells translational medicine |
| container_volume | 6 |
| creator | Abreu, Soraia C. Antunes, Mariana A. Xisto, Debora G. Cruz, Fernanda F. Branco, Vivian C. Bandeira, Elga Zola Kitoko, Jamil de Araújo, Almair F. Dellatorre‐Texeira, Ludmilla Olsen, Priscilla C. Weiss, Daniel J. Diaz, Bruno L. Morales, Marcelo M. Rocco, Patricia R. M. |
| description | Mesenchymal stromal cells (MSCs) from different sources have differential effects on lung injury. To compare the effects of murine MSCs from bone marrow (BM), adipose tissue (AD), and lung tissue (LUNG) on inflammatory and remodeling processes in experimental allergic asthma, female C57BL/6 mice were sensitized and challenged with ovalbumin (OVA) or saline (C). Twenty‐four hours after the last challenge, mice received either saline (50 µl, SAL), BM‐MSCs, AD‐MSCs, or LUNG‐MSCs (105 cells per mouse in 50 µl total volume) intratracheally. At 1 week, BM‐MSCs produced significantly greater reductions in resistive and viscoelastic pressures, bronchoconstriction index, collagen fiber content in lung parenchyma (but not airways), eosinophil infiltration, and levels of interleukin (IL)‐4, IL‐13, transforming growth factor (TGF)‐β, and vascular endothelial growth factor (VEGF) in lung homogenates compared to AD‐MSCs and LUNG‐MSCs. Only BM‐MSCs increased IL‐10 and interferon (IFN)‐γ in lung tissue. In parallel in vitro experiments, BM‐MSCs increased M2 macrophage polarization, whereas AD‐MSCs and LUNG‐MSCs had higher baseline levels of IL‐4, insulin‐like growth factor (IGF), and VEGF secretion. Exposure of MSCs to serum specimens obtained from asthmatic mice promoted reductions in secretion of these mediators, particularly in BM‐MSCs. Intratracheally administered BM‐MSCs, AD‐MSCs, and LUNG‐MSCs were differentially effective at reducing airway inflammation and remodeling and improving lung function in the current model of allergic asthma. In conclusion, intratracheal administration of MSCs from BM, AD, and LUNG were differentially effective at reducing airway inflammation and remodeling and improving lung function comparably reduced inflammation and fibrogenesis in this asthma model. However, altered lung mechanics and lung remodeling responded better to BM‐MSCs than to AD‐MSCs or LUNG‐MSCs. Moreover, each type of MSC was differentially affected in a surrogate in vitro model of the in vivo lung environment. Stem Cells Translational Medicine 2017;6:1557–1567
Bone marrow mesenchymal stromal cells (BM‐MSCs) reduced collagen fibers, eosinophils, and levels of interleukin (IL)‐4, IL‐13, transforming growth factor (TGF)‐β, and vascular endothelial growth factor (VEGF) compared to adipose tissue (AD)‐MSCs and LUNG‐MSCs. Only BM‐MSCs increased IL‐10 and interferon (IFN)‐γ in lung, and M2 macrophage polarization. These MSCs were differentially effective at reducing airway infl |
| doi_str_mv | 10.1002/sctm.16-0398 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5689762</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A735770498</galeid><sourcerecordid>A735770498</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5506-ec789d9a8e21a0592f08a59d938d60e0dd1e525493ba60e39d89daf9c0fe4ee43</originalsourceid><addsrcrecordid>eNp9kstu1DAUhiMEolXpjjWyxIbFpPgSO_YGKUzLRZoKRIe15SYnM66SeGonHbLjEXgcnocnwWGGKbDAm-PLd_5zjvUnyVOCzwjG9GUo-_aMiBQzJR8kx5TwPBVc4oeHvciOktMQbnBcQglF8ePkiMqMcp6L4-T7a9cBujTeu-0MFZXduAAzZLoKLYZuhZY2hAF-fP12Dt7eQYUuB2-nDAjQleuxNQ266r2b4hyaJqBP0IAJgM5tXYOHro9sZU3vfPgle7i3pmlGVMRT2aPC-q0Z7-t-NH6vj2yHLr5sYvk2JsUyRejXrXmSPKpNE-B0H0-Sz28ulvN36eLD2_fzYpGWnGORQplLVSkjgRKDuaI1lobHGyYrgQFXFQFOeabYtYlnpqqIm1qVuIYMIGMnyaud7ma4bqEqYw_eNHoT2zF-1M5Y_fdLZ9d65e40F1LlgkaBF3sB724HCL1ubSjjV5kO3BA0kYpgqTI2oc__QW_c4Ls4nqZUKiE4Y_ieWpkGtO1qF-uWk6gucsbzHGdKRmq2o0rvQvBQH1omWE_e0ZN3NBF68k7En_055gH-7ZQIsB2wtQ2M_xXTV_MlI9F_gv0EnL_TWA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2289665330</pqid></control><display><type>article</type><title>Bone Marrow, Adipose, and Lung Tissue‐Derived Murine Mesenchymal Stromal Cells Release Different Mediators and Differentially Affect Airway and Lung Parenchyma in Experimental Asthma</title><source>Oxford Journals Open Access Collection</source><source>Wiley Online Library Open Access</source><source>DOAJ Directory of Open Access Journals</source><source>Wiley Online Library Journals Frontfile Complete</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Abreu, Soraia C. ; Antunes, Mariana A. ; Xisto, Debora G. ; Cruz, Fernanda F. ; Branco, Vivian C. ; Bandeira, Elga ; Zola Kitoko, Jamil ; de Araújo, Almair F. ; Dellatorre‐Texeira, Ludmilla ; Olsen, Priscilla C. ; Weiss, Daniel J. ; Diaz, Bruno L. ; Morales, Marcelo M. ; Rocco, Patricia R. M.</creator><creatorcontrib>Abreu, Soraia C. ; Antunes, Mariana A. ; Xisto, Debora G. ; Cruz, Fernanda F. ; Branco, Vivian C. ; Bandeira, Elga ; Zola Kitoko, Jamil ; de Araújo, Almair F. ; Dellatorre‐Texeira, Ludmilla ; Olsen, Priscilla C. ; Weiss, Daniel J. ; Diaz, Bruno L. ; Morales, Marcelo M. ; Rocco, Patricia R. M.</creatorcontrib><description>Mesenchymal stromal cells (MSCs) from different sources have differential effects on lung injury. To compare the effects of murine MSCs from bone marrow (BM), adipose tissue (AD), and lung tissue (LUNG) on inflammatory and remodeling processes in experimental allergic asthma, female C57BL/6 mice were sensitized and challenged with ovalbumin (OVA) or saline (C). Twenty‐four hours after the last challenge, mice received either saline (50 µl, SAL), BM‐MSCs, AD‐MSCs, or LUNG‐MSCs (105 cells per mouse in 50 µl total volume) intratracheally. At 1 week, BM‐MSCs produced significantly greater reductions in resistive and viscoelastic pressures, bronchoconstriction index, collagen fiber content in lung parenchyma (but not airways), eosinophil infiltration, and levels of interleukin (IL)‐4, IL‐13, transforming growth factor (TGF)‐β, and vascular endothelial growth factor (VEGF) in lung homogenates compared to AD‐MSCs and LUNG‐MSCs. Only BM‐MSCs increased IL‐10 and interferon (IFN)‐γ in lung tissue. In parallel in vitro experiments, BM‐MSCs increased M2 macrophage polarization, whereas AD‐MSCs and LUNG‐MSCs had higher baseline levels of IL‐4, insulin‐like growth factor (IGF), and VEGF secretion. Exposure of MSCs to serum specimens obtained from asthmatic mice promoted reductions in secretion of these mediators, particularly in BM‐MSCs. Intratracheally administered BM‐MSCs, AD‐MSCs, and LUNG‐MSCs were differentially effective at reducing airway inflammation and remodeling and improving lung function in the current model of allergic asthma. In conclusion, intratracheal administration of MSCs from BM, AD, and LUNG were differentially effective at reducing airway inflammation and remodeling and improving lung function comparably reduced inflammation and fibrogenesis in this asthma model. However, altered lung mechanics and lung remodeling responded better to BM‐MSCs than to AD‐MSCs or LUNG‐MSCs. Moreover, each type of MSC was differentially affected in a surrogate in vitro model of the in vivo lung environment. Stem Cells Translational Medicine 2017;6:1557–1567
Bone marrow mesenchymal stromal cells (BM‐MSCs) reduced collagen fibers, eosinophils, and levels of interleukin (IL)‐4, IL‐13, transforming growth factor (TGF)‐β, and vascular endothelial growth factor (VEGF) compared to adipose tissue (AD)‐MSCs and LUNG‐MSCs. Only BM‐MSCs increased IL‐10 and interferon (IFN)‐γ in lung, and M2 macrophage polarization. These MSCs were differentially effective at reducing airway inflammation and remodeling in allergic asthma.</description><identifier>ISSN: 2157-6564</identifier><identifier>ISSN: 2157-6580</identifier><identifier>EISSN: 2157-6580</identifier><identifier>DOI: 10.1002/sctm.16-0398</identifier><identifier>PMID: 28425576</identifier><language>eng</language><publisher>United States: Oxford University Press</publisher><subject>Adipose Stem Cells/VSF ; Adipose tissue ; Advertising executives ; Analysis ; Angiogenesis ; Antibiotics ; Asthma ; Biomarkers ; Bone marrow ; Bone Marrow Stem Cells ; Bronchoconstriction ; Collagen ; Cytokines ; Disease Models (Animal/Cell) ; Ethylenediaminetetraacetic acid ; Experiments ; Fibrosis ; Growth factors ; Inflammation ; Inflammation / Inflammatory Disease ; Insulin ; Insulin-like growth factors ; Interferon ; Laboratory animals ; Leukocytes (eosinophilic) ; Lungs ; Macrophage ; Macrophages ; Mesenchymal Stem Cells ; Mesenchymal stromal cells ; Mesenchyme ; Ovalbumin ; Parenchyma ; Research funding ; Respiratory function ; Respiratory tract ; Respiratory tract diseases ; Stem cell transplantation ; Stem cells ; Stromal cells ; Studies ; Tissue Engineering and Regenerative Medicine ; Trachea ; Transforming growth factor ; Transforming growth factors ; Translational s and Reviews ; Vascular endothelial growth factor ; Viscoelasticity</subject><ispartof>Stem cells translational medicine, 2017-06, Vol.6 (6), p.1557-1567</ispartof><rights>2017 The Authors published by Wiley Periodicals, Inc. on behalf of AlphaMed Press</rights><rights>2017 The Authors Stem Cells Translational Medicine published by Wiley Periodicals, Inc. on behalf of AlphaMed Press.</rights><rights>COPYRIGHT 2017 Oxford University Press</rights><rights>2017. This work is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2017 The Authors S C T M published by Wiley Periodicals, Inc. on behalf of AlphaMed Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5506-ec789d9a8e21a0592f08a59d938d60e0dd1e525493ba60e39d89daf9c0fe4ee43</citedby><cites>FETCH-LOGICAL-c5506-ec789d9a8e21a0592f08a59d938d60e0dd1e525493ba60e39d89daf9c0fe4ee43</cites><orcidid>0000-0003-1412-7136</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5689762/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5689762/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,1411,11541,27901,27902,45550,45551,46027,46451,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28425576$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abreu, Soraia C.</creatorcontrib><creatorcontrib>Antunes, Mariana A.</creatorcontrib><creatorcontrib>Xisto, Debora G.</creatorcontrib><creatorcontrib>Cruz, Fernanda F.</creatorcontrib><creatorcontrib>Branco, Vivian C.</creatorcontrib><creatorcontrib>Bandeira, Elga</creatorcontrib><creatorcontrib>Zola Kitoko, Jamil</creatorcontrib><creatorcontrib>de Araújo, Almair F.</creatorcontrib><creatorcontrib>Dellatorre‐Texeira, Ludmilla</creatorcontrib><creatorcontrib>Olsen, Priscilla C.</creatorcontrib><creatorcontrib>Weiss, Daniel J.</creatorcontrib><creatorcontrib>Diaz, Bruno L.</creatorcontrib><creatorcontrib>Morales, Marcelo M.</creatorcontrib><creatorcontrib>Rocco, Patricia R. M.</creatorcontrib><title>Bone Marrow, Adipose, and Lung Tissue‐Derived Murine Mesenchymal Stromal Cells Release Different Mediators and Differentially Affect Airway and Lung Parenchyma in Experimental Asthma</title><title>Stem cells translational medicine</title><addtitle>Stem Cells Transl Med</addtitle><description>Mesenchymal stromal cells (MSCs) from different sources have differential effects on lung injury. To compare the effects of murine MSCs from bone marrow (BM), adipose tissue (AD), and lung tissue (LUNG) on inflammatory and remodeling processes in experimental allergic asthma, female C57BL/6 mice were sensitized and challenged with ovalbumin (OVA) or saline (C). Twenty‐four hours after the last challenge, mice received either saline (50 µl, SAL), BM‐MSCs, AD‐MSCs, or LUNG‐MSCs (105 cells per mouse in 50 µl total volume) intratracheally. At 1 week, BM‐MSCs produced significantly greater reductions in resistive and viscoelastic pressures, bronchoconstriction index, collagen fiber content in lung parenchyma (but not airways), eosinophil infiltration, and levels of interleukin (IL)‐4, IL‐13, transforming growth factor (TGF)‐β, and vascular endothelial growth factor (VEGF) in lung homogenates compared to AD‐MSCs and LUNG‐MSCs. Only BM‐MSCs increased IL‐10 and interferon (IFN)‐γ in lung tissue. In parallel in vitro experiments, BM‐MSCs increased M2 macrophage polarization, whereas AD‐MSCs and LUNG‐MSCs had higher baseline levels of IL‐4, insulin‐like growth factor (IGF), and VEGF secretion. Exposure of MSCs to serum specimens obtained from asthmatic mice promoted reductions in secretion of these mediators, particularly in BM‐MSCs. Intratracheally administered BM‐MSCs, AD‐MSCs, and LUNG‐MSCs were differentially effective at reducing airway inflammation and remodeling and improving lung function in the current model of allergic asthma. In conclusion, intratracheal administration of MSCs from BM, AD, and LUNG were differentially effective at reducing airway inflammation and remodeling and improving lung function comparably reduced inflammation and fibrogenesis in this asthma model. However, altered lung mechanics and lung remodeling responded better to BM‐MSCs than to AD‐MSCs or LUNG‐MSCs. Moreover, each type of MSC was differentially affected in a surrogate in vitro model of the in vivo lung environment. Stem Cells Translational Medicine 2017;6:1557–1567
Bone marrow mesenchymal stromal cells (BM‐MSCs) reduced collagen fibers, eosinophils, and levels of interleukin (IL)‐4, IL‐13, transforming growth factor (TGF)‐β, and vascular endothelial growth factor (VEGF) compared to adipose tissue (AD)‐MSCs and LUNG‐MSCs. Only BM‐MSCs increased IL‐10 and interferon (IFN)‐γ in lung, and M2 macrophage polarization. These MSCs were differentially effective at reducing airway inflammation and remodeling in allergic asthma.</description><subject>Adipose Stem Cells/VSF</subject><subject>Adipose tissue</subject><subject>Advertising executives</subject><subject>Analysis</subject><subject>Angiogenesis</subject><subject>Antibiotics</subject><subject>Asthma</subject><subject>Biomarkers</subject><subject>Bone marrow</subject><subject>Bone Marrow Stem Cells</subject><subject>Bronchoconstriction</subject><subject>Collagen</subject><subject>Cytokines</subject><subject>Disease Models (Animal/Cell)</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Experiments</subject><subject>Fibrosis</subject><subject>Growth factors</subject><subject>Inflammation</subject><subject>Inflammation / Inflammatory Disease</subject><subject>Insulin</subject><subject>Insulin-like growth factors</subject><subject>Interferon</subject><subject>Laboratory animals</subject><subject>Leukocytes (eosinophilic)</subject><subject>Lungs</subject><subject>Macrophage</subject><subject>Macrophages</subject><subject>Mesenchymal Stem Cells</subject><subject>Mesenchymal stromal cells</subject><subject>Mesenchyme</subject><subject>Ovalbumin</subject><subject>Parenchyma</subject><subject>Research funding</subject><subject>Respiratory function</subject><subject>Respiratory tract</subject><subject>Respiratory tract diseases</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Stromal cells</subject><subject>Studies</subject><subject>Tissue Engineering and Regenerative Medicine</subject><subject>Trachea</subject><subject>Transforming growth factor</subject><subject>Transforming growth factors</subject><subject>Translational s and Reviews</subject><subject>Vascular endothelial growth factor</subject><subject>Viscoelasticity</subject><issn>2157-6564</issn><issn>2157-6580</issn><issn>2157-6580</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kstu1DAUhiMEolXpjjWyxIbFpPgSO_YGKUzLRZoKRIe15SYnM66SeGonHbLjEXgcnocnwWGGKbDAm-PLd_5zjvUnyVOCzwjG9GUo-_aMiBQzJR8kx5TwPBVc4oeHvciOktMQbnBcQglF8ePkiMqMcp6L4-T7a9cBujTeu-0MFZXduAAzZLoKLYZuhZY2hAF-fP12Dt7eQYUuB2-nDAjQleuxNQ266r2b4hyaJqBP0IAJgM5tXYOHro9sZU3vfPgle7i3pmlGVMRT2aPC-q0Z7-t-NH6vj2yHLr5sYvk2JsUyRejXrXmSPKpNE-B0H0-Sz28ulvN36eLD2_fzYpGWnGORQplLVSkjgRKDuaI1lobHGyYrgQFXFQFOeabYtYlnpqqIm1qVuIYMIGMnyaud7ma4bqEqYw_eNHoT2zF-1M5Y_fdLZ9d65e40F1LlgkaBF3sB724HCL1ubSjjV5kO3BA0kYpgqTI2oc__QW_c4Ls4nqZUKiE4Y_ieWpkGtO1qF-uWk6gucsbzHGdKRmq2o0rvQvBQH1omWE_e0ZN3NBF68k7En_055gH-7ZQIsB2wtQ2M_xXTV_MlI9F_gv0EnL_TWA</recordid><startdate>201706</startdate><enddate>201706</enddate><creator>Abreu, Soraia C.</creator><creator>Antunes, Mariana A.</creator><creator>Xisto, Debora G.</creator><creator>Cruz, Fernanda F.</creator><creator>Branco, Vivian C.</creator><creator>Bandeira, Elga</creator><creator>Zola Kitoko, Jamil</creator><creator>de Araújo, Almair F.</creator><creator>Dellatorre‐Texeira, Ludmilla</creator><creator>Olsen, Priscilla C.</creator><creator>Weiss, Daniel J.</creator><creator>Diaz, Bruno L.</creator><creator>Morales, Marcelo M.</creator><creator>Rocco, Patricia R. M.</creator><general>Oxford University Press</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1412-7136</orcidid></search><sort><creationdate>201706</creationdate><title>Bone Marrow, Adipose, and Lung Tissue‐Derived Murine Mesenchymal Stromal Cells Release Different Mediators and Differentially Affect Airway and Lung Parenchyma in Experimental Asthma</title><author>Abreu, Soraia C. ; Antunes, Mariana A. ; Xisto, Debora G. ; Cruz, Fernanda F. ; Branco, Vivian C. ; Bandeira, Elga ; Zola Kitoko, Jamil ; de Araújo, Almair F. ; Dellatorre‐Texeira, Ludmilla ; Olsen, Priscilla C. ; Weiss, Daniel J. ; Diaz, Bruno L. ; Morales, Marcelo M. ; Rocco, Patricia R. M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5506-ec789d9a8e21a0592f08a59d938d60e0dd1e525493ba60e39d89daf9c0fe4ee43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adipose Stem Cells/VSF</topic><topic>Adipose tissue</topic><topic>Advertising executives</topic><topic>Analysis</topic><topic>Angiogenesis</topic><topic>Antibiotics</topic><topic>Asthma</topic><topic>Biomarkers</topic><topic>Bone marrow</topic><topic>Bone Marrow Stem Cells</topic><topic>Bronchoconstriction</topic><topic>Collagen</topic><topic>Cytokines</topic><topic>Disease Models (Animal/Cell)</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Experiments</topic><topic>Fibrosis</topic><topic>Growth factors</topic><topic>Inflammation</topic><topic>Inflammation / Inflammatory Disease</topic><topic>Insulin</topic><topic>Insulin-like growth factors</topic><topic>Interferon</topic><topic>Laboratory animals</topic><topic>Leukocytes (eosinophilic)</topic><topic>Lungs</topic><topic>Macrophage</topic><topic>Macrophages</topic><topic>Mesenchymal Stem Cells</topic><topic>Mesenchymal stromal cells</topic><topic>Mesenchyme</topic><topic>Ovalbumin</topic><topic>Parenchyma</topic><topic>Research funding</topic><topic>Respiratory function</topic><topic>Respiratory tract</topic><topic>Respiratory tract diseases</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Stromal cells</topic><topic>Studies</topic><topic>Tissue Engineering and Regenerative Medicine</topic><topic>Trachea</topic><topic>Transforming growth factor</topic><topic>Transforming growth factors</topic><topic>Translational s and Reviews</topic><topic>Vascular endothelial growth factor</topic><topic>Viscoelasticity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abreu, Soraia C.</creatorcontrib><creatorcontrib>Antunes, Mariana A.</creatorcontrib><creatorcontrib>Xisto, Debora G.</creatorcontrib><creatorcontrib>Cruz, Fernanda F.</creatorcontrib><creatorcontrib>Branco, Vivian C.</creatorcontrib><creatorcontrib>Bandeira, Elga</creatorcontrib><creatorcontrib>Zola Kitoko, Jamil</creatorcontrib><creatorcontrib>de Araújo, Almair F.</creatorcontrib><creatorcontrib>Dellatorre‐Texeira, Ludmilla</creatorcontrib><creatorcontrib>Olsen, Priscilla C.</creatorcontrib><creatorcontrib>Weiss, Daniel J.</creatorcontrib><creatorcontrib>Diaz, Bruno L.</creatorcontrib><creatorcontrib>Morales, Marcelo M.</creatorcontrib><creatorcontrib>Rocco, Patricia R. M.</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Stem cells translational medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abreu, Soraia C.</au><au>Antunes, Mariana A.</au><au>Xisto, Debora G.</au><au>Cruz, Fernanda F.</au><au>Branco, Vivian C.</au><au>Bandeira, Elga</au><au>Zola Kitoko, Jamil</au><au>de Araújo, Almair F.</au><au>Dellatorre‐Texeira, Ludmilla</au><au>Olsen, Priscilla C.</au><au>Weiss, Daniel J.</au><au>Diaz, Bruno L.</au><au>Morales, Marcelo M.</au><au>Rocco, Patricia R. M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bone Marrow, Adipose, and Lung Tissue‐Derived Murine Mesenchymal Stromal Cells Release Different Mediators and Differentially Affect Airway and Lung Parenchyma in Experimental Asthma</atitle><jtitle>Stem cells translational medicine</jtitle><addtitle>Stem Cells Transl Med</addtitle><date>2017-06</date><risdate>2017</risdate><volume>6</volume><issue>6</issue><spage>1557</spage><epage>1567</epage><pages>1557-1567</pages><issn>2157-6564</issn><issn>2157-6580</issn><eissn>2157-6580</eissn><abstract>Mesenchymal stromal cells (MSCs) from different sources have differential effects on lung injury. To compare the effects of murine MSCs from bone marrow (BM), adipose tissue (AD), and lung tissue (LUNG) on inflammatory and remodeling processes in experimental allergic asthma, female C57BL/6 mice were sensitized and challenged with ovalbumin (OVA) or saline (C). Twenty‐four hours after the last challenge, mice received either saline (50 µl, SAL), BM‐MSCs, AD‐MSCs, or LUNG‐MSCs (105 cells per mouse in 50 µl total volume) intratracheally. At 1 week, BM‐MSCs produced significantly greater reductions in resistive and viscoelastic pressures, bronchoconstriction index, collagen fiber content in lung parenchyma (but not airways), eosinophil infiltration, and levels of interleukin (IL)‐4, IL‐13, transforming growth factor (TGF)‐β, and vascular endothelial growth factor (VEGF) in lung homogenates compared to AD‐MSCs and LUNG‐MSCs. Only BM‐MSCs increased IL‐10 and interferon (IFN)‐γ in lung tissue. In parallel in vitro experiments, BM‐MSCs increased M2 macrophage polarization, whereas AD‐MSCs and LUNG‐MSCs had higher baseline levels of IL‐4, insulin‐like growth factor (IGF), and VEGF secretion. Exposure of MSCs to serum specimens obtained from asthmatic mice promoted reductions in secretion of these mediators, particularly in BM‐MSCs. Intratracheally administered BM‐MSCs, AD‐MSCs, and LUNG‐MSCs were differentially effective at reducing airway inflammation and remodeling and improving lung function in the current model of allergic asthma. In conclusion, intratracheal administration of MSCs from BM, AD, and LUNG were differentially effective at reducing airway inflammation and remodeling and improving lung function comparably reduced inflammation and fibrogenesis in this asthma model. However, altered lung mechanics and lung remodeling responded better to BM‐MSCs than to AD‐MSCs or LUNG‐MSCs. Moreover, each type of MSC was differentially affected in a surrogate in vitro model of the in vivo lung environment. Stem Cells Translational Medicine 2017;6:1557–1567
Bone marrow mesenchymal stromal cells (BM‐MSCs) reduced collagen fibers, eosinophils, and levels of interleukin (IL)‐4, IL‐13, transforming growth factor (TGF)‐β, and vascular endothelial growth factor (VEGF) compared to adipose tissue (AD)‐MSCs and LUNG‐MSCs. Only BM‐MSCs increased IL‐10 and interferon (IFN)‐γ in lung, and M2 macrophage polarization. These MSCs were differentially effective at reducing airway inflammation and remodeling in allergic asthma.</abstract><cop>United States</cop><pub>Oxford University Press</pub><pmid>28425576</pmid><doi>10.1002/sctm.16-0398</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-1412-7136</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2157-6564 |
| ispartof | Stem cells translational medicine, 2017-06, Vol.6 (6), p.1557-1567 |
| issn | 2157-6564 2157-6580 2157-6580 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5689762 |
| source | Oxford Journals Open Access Collection; Wiley Online Library Open Access; DOAJ Directory of Open Access Journals; Wiley Online Library Journals Frontfile Complete; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Adipose Stem Cells/VSF Adipose tissue Advertising executives Analysis Angiogenesis Antibiotics Asthma Biomarkers Bone marrow Bone Marrow Stem Cells Bronchoconstriction Collagen Cytokines Disease Models (Animal/Cell) Ethylenediaminetetraacetic acid Experiments Fibrosis Growth factors Inflammation Inflammation / Inflammatory Disease Insulin Insulin-like growth factors Interferon Laboratory animals Leukocytes (eosinophilic) Lungs Macrophage Macrophages Mesenchymal Stem Cells Mesenchymal stromal cells Mesenchyme Ovalbumin Parenchyma Research funding Respiratory function Respiratory tract Respiratory tract diseases Stem cell transplantation Stem cells Stromal cells Studies Tissue Engineering and Regenerative Medicine Trachea Transforming growth factor Transforming growth factors Translational s and Reviews Vascular endothelial growth factor Viscoelasticity |
| title | Bone Marrow, Adipose, and Lung Tissue‐Derived Murine Mesenchymal Stromal Cells Release Different Mediators and Differentially Affect Airway and Lung Parenchyma in Experimental Asthma |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T03%3A56%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bone%20Marrow,%20Adipose,%20and%20Lung%20Tissue%E2%80%90Derived%20Murine%20Mesenchymal%20Stromal%20Cells%20Release%20Different%20Mediators%20and%20Differentially%20Affect%20Airway%20and%20Lung%20Parenchyma%20in%20Experimental%20Asthma&rft.jtitle=Stem%20cells%20translational%20medicine&rft.au=Abreu,%20Soraia%20C.&rft.date=2017-06&rft.volume=6&rft.issue=6&rft.spage=1557&rft.epage=1567&rft.pages=1557-1567&rft.issn=2157-6564&rft.eissn=2157-6580&rft_id=info:doi/10.1002/sctm.16-0398&rft_dat=%3Cgale_pubme%3EA735770498%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2289665330&rft_id=info:pmid/28425576&rft_galeid=A735770498&rfr_iscdi=true |