Neutrophils in chronic lymphocytic leukemia are permanently activated and have functional defects
A growing body of studies highlights involvement of neutrophils in cancer development and progression. Our aim was to assess the phenotypic and functional properties of circulating neutrophils from patients with chronic lymphocytic leukemia (CLL). The percentage of CD54+ and CD64+ neutrophils as wel...
Gespeichert in:
| Veröffentlicht in: | Oncotarget 2017-10, Vol.8 (49), p.84889-84901 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 84901 |
|---|---|
| container_issue | 49 |
| container_start_page | 84889 |
| container_title | Oncotarget |
| container_volume | 8 |
| creator | Manukyan, Gayane Papajik, Tomas Gajdos, Petr Mikulkova, Zuzana Urbanova, Renata Gabcova, Gabriela Kudelka, Milos Turcsányi, Peter Ryznerova, Pavlina Prochazka, Vit Kriegova, Eva |
| description | A growing body of studies highlights involvement of neutrophils in cancer development and progression. Our aim was to assess the phenotypic and functional properties of circulating neutrophils from patients with chronic lymphocytic leukemia (CLL). The percentage of CD54+ and CD64+ neutrophils as well as CD54 expression on these cells were higher in CLL patients than in age-matched healthy controls. Neutrophils from CLL produced more reactive oxygen species (ROS) compared to controls in both resting and activated conditions. Lipopolysaccharide-induced production of IL-1β and TNF-a as well as reduced TLR2 expression in neutrophils from CLL than in neutrophils from controls suggesting their tolerant state. Finally, phenotypic alterations of neutrophils, particularly elevation of CD64 and CD54 markers, correlated with disease activity and treatment, and low percentage of neutrophils. Taken together, the alterations in percentage and functional characteristics of neutrophils reflect the clinical course of CLL. Our data provide first evidence that neutrophils in CLL are permanently primed and have functional defects. |
| doi_str_mv | 10.18632/oncotarget.20031 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5689581</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1966983935</sourcerecordid><originalsourceid>FETCH-LOGICAL-c356t-2f638a819f6c7da810f17bb95090963fce654e48e229910e301b4eaf6dd15a0f3</originalsourceid><addsrcrecordid>eNpVUctO3TAQtVARIMoHsKm87OaCHce-8aZShVpAQrApa2uuMyamiZ3azpXu3-PyKp3NHM3jzOMQcsrZGe-UaM5jsLFAesBy1jAm-B454rrVq0ZK8ekDPiQnOT-yarJdd40-IIeN5lIpzY8I3OJSUpwHP2bqA7VDisFbOu6meYh2V_5iXH7j5IFCQjpjmiBgKOOOgi1-CwV7CqGnA2yRuiXUYAww0h4d2pI_k30HY8aTV39M7n_--HVxtbq5u7y--H6zskKqsmqcEh10XDtl130FzPH1ZqMl00wr4Swq2WLbYdNozRkKxjctglN9zyUwJ47JtxfeedlM2Nu6YoLRzMlPkHYmgjf_Z4IfzEPcGqk6LTteCb6-EqT4Z8FczOSzxXGs58YlG67ryzqhhayl_KXUpphzQvc-hjPzrI75p455Vqf2fPm433vHmxbiCeEXkSI</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1966983935</pqid></control><display><type>article</type><title>Neutrophils in chronic lymphocytic leukemia are permanently activated and have functional defects</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>PubMed Central</source><source>Free E- Journals</source><creator>Manukyan, Gayane ; Papajik, Tomas ; Gajdos, Petr ; Mikulkova, Zuzana ; Urbanova, Renata ; Gabcova, Gabriela ; Kudelka, Milos ; Turcsányi, Peter ; Ryznerova, Pavlina ; Prochazka, Vit ; Kriegova, Eva</creator><creatorcontrib>Manukyan, Gayane ; Papajik, Tomas ; Gajdos, Petr ; Mikulkova, Zuzana ; Urbanova, Renata ; Gabcova, Gabriela ; Kudelka, Milos ; Turcsányi, Peter ; Ryznerova, Pavlina ; Prochazka, Vit ; Kriegova, Eva</creatorcontrib><description>A growing body of studies highlights involvement of neutrophils in cancer development and progression. Our aim was to assess the phenotypic and functional properties of circulating neutrophils from patients with chronic lymphocytic leukemia (CLL). The percentage of CD54+ and CD64+ neutrophils as well as CD54 expression on these cells were higher in CLL patients than in age-matched healthy controls. Neutrophils from CLL produced more reactive oxygen species (ROS) compared to controls in both resting and activated conditions. Lipopolysaccharide-induced production of IL-1β and TNF-a as well as reduced TLR2 expression in neutrophils from CLL than in neutrophils from controls suggesting their tolerant state. Finally, phenotypic alterations of neutrophils, particularly elevation of CD64 and CD54 markers, correlated with disease activity and treatment, and low percentage of neutrophils. Taken together, the alterations in percentage and functional characteristics of neutrophils reflect the clinical course of CLL. Our data provide first evidence that neutrophils in CLL are permanently primed and have functional defects.</description><identifier>ISSN: 1949-2553</identifier><identifier>EISSN: 1949-2553</identifier><identifier>DOI: 10.18632/oncotarget.20031</identifier><identifier>PMID: 29156691</identifier><language>eng</language><publisher>United States: Impact Journals LLC</publisher><subject>Research Paper</subject><ispartof>Oncotarget, 2017-10, Vol.8 (49), p.84889-84901</ispartof><rights>Copyright: © 2017 Manukyan et al. 2017</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-2f638a819f6c7da810f17bb95090963fce654e48e229910e301b4eaf6dd15a0f3</citedby><cites>FETCH-LOGICAL-c356t-2f638a819f6c7da810f17bb95090963fce654e48e229910e301b4eaf6dd15a0f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5689581/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5689581/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29156691$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Manukyan, Gayane</creatorcontrib><creatorcontrib>Papajik, Tomas</creatorcontrib><creatorcontrib>Gajdos, Petr</creatorcontrib><creatorcontrib>Mikulkova, Zuzana</creatorcontrib><creatorcontrib>Urbanova, Renata</creatorcontrib><creatorcontrib>Gabcova, Gabriela</creatorcontrib><creatorcontrib>Kudelka, Milos</creatorcontrib><creatorcontrib>Turcsányi, Peter</creatorcontrib><creatorcontrib>Ryznerova, Pavlina</creatorcontrib><creatorcontrib>Prochazka, Vit</creatorcontrib><creatorcontrib>Kriegova, Eva</creatorcontrib><title>Neutrophils in chronic lymphocytic leukemia are permanently activated and have functional defects</title><title>Oncotarget</title><addtitle>Oncotarget</addtitle><description>A growing body of studies highlights involvement of neutrophils in cancer development and progression. Our aim was to assess the phenotypic and functional properties of circulating neutrophils from patients with chronic lymphocytic leukemia (CLL). The percentage of CD54+ and CD64+ neutrophils as well as CD54 expression on these cells were higher in CLL patients than in age-matched healthy controls. Neutrophils from CLL produced more reactive oxygen species (ROS) compared to controls in both resting and activated conditions. Lipopolysaccharide-induced production of IL-1β and TNF-a as well as reduced TLR2 expression in neutrophils from CLL than in neutrophils from controls suggesting their tolerant state. Finally, phenotypic alterations of neutrophils, particularly elevation of CD64 and CD54 markers, correlated with disease activity and treatment, and low percentage of neutrophils. Taken together, the alterations in percentage and functional characteristics of neutrophils reflect the clinical course of CLL. Our data provide first evidence that neutrophils in CLL are permanently primed and have functional defects.</description><subject>Research Paper</subject><issn>1949-2553</issn><issn>1949-2553</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNpVUctO3TAQtVARIMoHsKm87OaCHce-8aZShVpAQrApa2uuMyamiZ3azpXu3-PyKp3NHM3jzOMQcsrZGe-UaM5jsLFAesBy1jAm-B454rrVq0ZK8ekDPiQnOT-yarJdd40-IIeN5lIpzY8I3OJSUpwHP2bqA7VDisFbOu6meYh2V_5iXH7j5IFCQjpjmiBgKOOOgi1-CwV7CqGnA2yRuiXUYAww0h4d2pI_k30HY8aTV39M7n_--HVxtbq5u7y--H6zskKqsmqcEh10XDtl130FzPH1ZqMl00wr4Swq2WLbYdNozRkKxjctglN9zyUwJ47JtxfeedlM2Nu6YoLRzMlPkHYmgjf_Z4IfzEPcGqk6LTteCb6-EqT4Z8FczOSzxXGs58YlG67ryzqhhayl_KXUpphzQvc-hjPzrI75p455Vqf2fPm433vHmxbiCeEXkSI</recordid><startdate>20171017</startdate><enddate>20171017</enddate><creator>Manukyan, Gayane</creator><creator>Papajik, Tomas</creator><creator>Gajdos, Petr</creator><creator>Mikulkova, Zuzana</creator><creator>Urbanova, Renata</creator><creator>Gabcova, Gabriela</creator><creator>Kudelka, Milos</creator><creator>Turcsányi, Peter</creator><creator>Ryznerova, Pavlina</creator><creator>Prochazka, Vit</creator><creator>Kriegova, Eva</creator><general>Impact Journals LLC</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20171017</creationdate><title>Neutrophils in chronic lymphocytic leukemia are permanently activated and have functional defects</title><author>Manukyan, Gayane ; Papajik, Tomas ; Gajdos, Petr ; Mikulkova, Zuzana ; Urbanova, Renata ; Gabcova, Gabriela ; Kudelka, Milos ; Turcsányi, Peter ; Ryznerova, Pavlina ; Prochazka, Vit ; Kriegova, Eva</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-2f638a819f6c7da810f17bb95090963fce654e48e229910e301b4eaf6dd15a0f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Research Paper</topic><toplevel>online_resources</toplevel><creatorcontrib>Manukyan, Gayane</creatorcontrib><creatorcontrib>Papajik, Tomas</creatorcontrib><creatorcontrib>Gajdos, Petr</creatorcontrib><creatorcontrib>Mikulkova, Zuzana</creatorcontrib><creatorcontrib>Urbanova, Renata</creatorcontrib><creatorcontrib>Gabcova, Gabriela</creatorcontrib><creatorcontrib>Kudelka, Milos</creatorcontrib><creatorcontrib>Turcsányi, Peter</creatorcontrib><creatorcontrib>Ryznerova, Pavlina</creatorcontrib><creatorcontrib>Prochazka, Vit</creatorcontrib><creatorcontrib>Kriegova, Eva</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Oncotarget</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Manukyan, Gayane</au><au>Papajik, Tomas</au><au>Gajdos, Petr</au><au>Mikulkova, Zuzana</au><au>Urbanova, Renata</au><au>Gabcova, Gabriela</au><au>Kudelka, Milos</au><au>Turcsányi, Peter</au><au>Ryznerova, Pavlina</au><au>Prochazka, Vit</au><au>Kriegova, Eva</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neutrophils in chronic lymphocytic leukemia are permanently activated and have functional defects</atitle><jtitle>Oncotarget</jtitle><addtitle>Oncotarget</addtitle><date>2017-10-17</date><risdate>2017</risdate><volume>8</volume><issue>49</issue><spage>84889</spage><epage>84901</epage><pages>84889-84901</pages><issn>1949-2553</issn><eissn>1949-2553</eissn><abstract>A growing body of studies highlights involvement of neutrophils in cancer development and progression. Our aim was to assess the phenotypic and functional properties of circulating neutrophils from patients with chronic lymphocytic leukemia (CLL). The percentage of CD54+ and CD64+ neutrophils as well as CD54 expression on these cells were higher in CLL patients than in age-matched healthy controls. Neutrophils from CLL produced more reactive oxygen species (ROS) compared to controls in both resting and activated conditions. Lipopolysaccharide-induced production of IL-1β and TNF-a as well as reduced TLR2 expression in neutrophils from CLL than in neutrophils from controls suggesting their tolerant state. Finally, phenotypic alterations of neutrophils, particularly elevation of CD64 and CD54 markers, correlated with disease activity and treatment, and low percentage of neutrophils. Taken together, the alterations in percentage and functional characteristics of neutrophils reflect the clinical course of CLL. Our data provide first evidence that neutrophils in CLL are permanently primed and have functional defects.</abstract><cop>United States</cop><pub>Impact Journals LLC</pub><pmid>29156691</pmid><doi>10.18632/oncotarget.20031</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1949-2553 |
| ispartof | Oncotarget, 2017-10, Vol.8 (49), p.84889-84901 |
| issn | 1949-2553 1949-2553 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5689581 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; PubMed Central; Free E- Journals |
| subjects | Research Paper |
| title | Neutrophils in chronic lymphocytic leukemia are permanently activated and have functional defects |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T08%3A31%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Neutrophils%20in%20chronic%20lymphocytic%20leukemia%20are%20permanently%20activated%20and%20have%20functional%20defects&rft.jtitle=Oncotarget&rft.au=Manukyan,%20Gayane&rft.date=2017-10-17&rft.volume=8&rft.issue=49&rft.spage=84889&rft.epage=84901&rft.pages=84889-84901&rft.issn=1949-2553&rft.eissn=1949-2553&rft_id=info:doi/10.18632/oncotarget.20031&rft_dat=%3Cproquest_pubme%3E1966983935%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1966983935&rft_id=info:pmid/29156691&rfr_iscdi=true |