Nanofiber-based sutures induce endogenous antimicrobial peptide
The aim of this study was to develop nanofiber-based sutures capable of inducing endogenous antimicrobial peptide production. We used co-axial electrospinning deposition and rolling to fabricate sutures containing pam3CSK4 peptide and 25-hydroxyvitamin D (25D ). The diameters and mechanical properti...
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| Veröffentlicht in: | Nanomedicine (London, England) England), 2017-11, Vol.12 (21), p.2597-2609 |
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| creator | Chen, Shixuan Ge, Liangpeng Gombart, Adrian F Shuler, Franklin D Carlson, Mark A Reilly, Debra A Xie, Jingwei |
| description | The aim of this study was to develop nanofiber-based sutures capable of inducing endogenous antimicrobial peptide production.
We used co-axial electrospinning deposition and rolling to fabricate sutures containing pam3CSK4 peptide and 25-hydroxyvitamin D
(25D
).
The diameters and mechanical properties of the sutures were adjustable to meet the criteria of United States Pharmacopeia designation. 25D
exhibited a sustained release from nanofiber sutures over 4 weeks. Pam3CSK4 peptide also showed an initial burst followed by a sustained release over 4 weeks. The co-delivery of 25D
and pam3CSK4 peptide enhanced cathelicidin antimicrobial peptide production from U937 cells and keratinocytes compared with 25D
delivery alone. In addition, the 25D
/pam3CSK4 peptide co-loaded nanofiber sutures did not significantly influence proliferation of keratinocytes, fibroblasts, or the monocytic cell lines U937 and HL-60.
The use of 25D
/pam3CSK4 peptide co-loaded nanofiber sutures could potentially induce endogenous antimicrobial peptide production and reduce surgical site infections. |
| doi_str_mv | 10.2217/nnm-2017-0161 |
| format | Article |
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We used co-axial electrospinning deposition and rolling to fabricate sutures containing pam3CSK4 peptide and 25-hydroxyvitamin D
(25D
).
The diameters and mechanical properties of the sutures were adjustable to meet the criteria of United States Pharmacopeia designation. 25D
exhibited a sustained release from nanofiber sutures over 4 weeks. Pam3CSK4 peptide also showed an initial burst followed by a sustained release over 4 weeks. The co-delivery of 25D
and pam3CSK4 peptide enhanced cathelicidin antimicrobial peptide production from U937 cells and keratinocytes compared with 25D
delivery alone. In addition, the 25D
/pam3CSK4 peptide co-loaded nanofiber sutures did not significantly influence proliferation of keratinocytes, fibroblasts, or the monocytic cell lines U937 and HL-60.
The use of 25D
/pam3CSK4 peptide co-loaded nanofiber sutures could potentially induce endogenous antimicrobial peptide production and reduce surgical site infections.</description><identifier>ISSN: 1743-5889</identifier><identifier>EISSN: 1748-6963</identifier><identifier>DOI: 10.2217/nnm-2017-0161</identifier><identifier>PMID: 28960168</identifier><language>eng</language><publisher>England: Future Medicine Ltd</publisher><subject>25-hydroxyvitamin D ; Antibiotics ; Antimicrobial agents ; Antimicrobial Cationic Peptides - biosynthesis ; Biomechanical Phenomena ; Calcifediol - chemistry ; Calcifediol - pharmacology ; Cell Line ; Cell Proliferation ; co-delivery ; Cost control ; Drug Carriers ; Drug Liberation ; electrospun nanofibers ; Fibroblasts - cytology ; Fibroblasts - metabolism ; Gene expression ; Humans ; Infections ; Injuries ; Keratinocytes - cytology ; Keratinocytes - metabolism ; Lipopeptides - chemistry ; Lipopeptides - pharmacology ; Nanofibers - chemistry ; pam3Cys-Ser-(Lys)4 peptide ; Particle Size ; Peptides ; Proteins ; Silver ; Skin - metabolism ; Surface Properties ; surgical site infection ; Surgical site infections ; Sutures ; Vitamin D ; Vitamin deficiency</subject><ispartof>Nanomedicine (London, England), 2017-11, Vol.12 (21), p.2597-2609</ispartof><rights>2017 Future Medicine Ltd</rights><rights>Copyright Future Medicine Ltd Nov 2017</rights><rights>2017 Future Medicine Ltd 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-baa711d8e256189c84c90d9fe7ebc1173b572c3529657623b3a21260a804047b3</citedby><cites>FETCH-LOGICAL-c465t-baa711d8e256189c84c90d9fe7ebc1173b572c3529657623b3a21260a804047b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5674207/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5674207/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,725,778,782,883,27911,27912,53778,53780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28960168$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Shixuan</creatorcontrib><creatorcontrib>Ge, Liangpeng</creatorcontrib><creatorcontrib>Gombart, Adrian F</creatorcontrib><creatorcontrib>Shuler, Franklin D</creatorcontrib><creatorcontrib>Carlson, Mark A</creatorcontrib><creatorcontrib>Reilly, Debra A</creatorcontrib><creatorcontrib>Xie, Jingwei</creatorcontrib><title>Nanofiber-based sutures induce endogenous antimicrobial peptide</title><title>Nanomedicine (London, England)</title><addtitle>Nanomedicine (Lond)</addtitle><description>The aim of this study was to develop nanofiber-based sutures capable of inducing endogenous antimicrobial peptide production.
We used co-axial electrospinning deposition and rolling to fabricate sutures containing pam3CSK4 peptide and 25-hydroxyvitamin D
(25D
).
The diameters and mechanical properties of the sutures were adjustable to meet the criteria of United States Pharmacopeia designation. 25D
exhibited a sustained release from nanofiber sutures over 4 weeks. Pam3CSK4 peptide also showed an initial burst followed by a sustained release over 4 weeks. The co-delivery of 25D
and pam3CSK4 peptide enhanced cathelicidin antimicrobial peptide production from U937 cells and keratinocytes compared with 25D
delivery alone. In addition, the 25D
/pam3CSK4 peptide co-loaded nanofiber sutures did not significantly influence proliferation of keratinocytes, fibroblasts, or the monocytic cell lines U937 and HL-60.
The use of 25D
/pam3CSK4 peptide co-loaded nanofiber sutures could potentially induce endogenous antimicrobial peptide production and reduce surgical site infections.</description><subject>25-hydroxyvitamin D</subject><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Antimicrobial Cationic Peptides - biosynthesis</subject><subject>Biomechanical Phenomena</subject><subject>Calcifediol - chemistry</subject><subject>Calcifediol - pharmacology</subject><subject>Cell Line</subject><subject>Cell Proliferation</subject><subject>co-delivery</subject><subject>Cost control</subject><subject>Drug Carriers</subject><subject>Drug Liberation</subject><subject>electrospun nanofibers</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - metabolism</subject><subject>Gene expression</subject><subject>Humans</subject><subject>Infections</subject><subject>Injuries</subject><subject>Keratinocytes - cytology</subject><subject>Keratinocytes - metabolism</subject><subject>Lipopeptides - chemistry</subject><subject>Lipopeptides - pharmacology</subject><subject>Nanofibers - chemistry</subject><subject>pam3Cys-Ser-(Lys)4 peptide</subject><subject>Particle Size</subject><subject>Peptides</subject><subject>Proteins</subject><subject>Silver</subject><subject>Skin - metabolism</subject><subject>Surface Properties</subject><subject>surgical site infection</subject><subject>Surgical site infections</subject><subject>Sutures</subject><subject>Vitamin D</subject><subject>Vitamin deficiency</subject><issn>1743-5889</issn><issn>1748-6963</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp1kD1PwzAQhi0EoqUwsqJIzAHbSfyxgFDFl1TBArPlOJfiqnGCnSDx73FJqWBgsq17_N7dg9ApwReUEn7pXJNSTHiKCSN7aEp4LlImWbb_fc_SQgg5QUchrDAuBCX4EE2okCzyYoqun7Rra1uCT0sdoErC0A8eQmJdNRhIwFXtElw7hES73jbW-La0ep100PW2gmN0UOt1gJPtOUOvd7cv84d08Xz_OL9ZpCZnRR-zNSekEkALRoQ0IjcSV7IGDqUhhGdlwanJCipZwRnNykxTQhnWAuc452U2Q1djbjeUDVQGXO_1WnXeNtp_qlZb9bfi7Jtath-qYDynmMeA822Ab98HCL1atYN3cWZFM0qYFITlkUpHKq4Zgod614FgtfGtom-18a02viN_9nusHf0jOAJyBOrRq7HgDKjxFX9YYx38E_4FkRSPtQ</recordid><startdate>20171101</startdate><enddate>20171101</enddate><creator>Chen, Shixuan</creator><creator>Ge, Liangpeng</creator><creator>Gombart, Adrian F</creator><creator>Shuler, Franklin D</creator><creator>Carlson, Mark A</creator><creator>Reilly, Debra A</creator><creator>Xie, Jingwei</creator><general>Future Medicine Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>EHMNL</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope></search><sort><creationdate>20171101</creationdate><title>Nanofiber-based sutures induce endogenous antimicrobial peptide</title><author>Chen, Shixuan ; Ge, Liangpeng ; Gombart, Adrian F ; Shuler, Franklin D ; Carlson, Mark A ; Reilly, Debra A ; Xie, Jingwei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-baa711d8e256189c84c90d9fe7ebc1173b572c3529657623b3a21260a804047b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>25-hydroxyvitamin D</topic><topic>Antibiotics</topic><topic>Antimicrobial agents</topic><topic>Antimicrobial Cationic Peptides - biosynthesis</topic><topic>Biomechanical Phenomena</topic><topic>Calcifediol - chemistry</topic><topic>Calcifediol - pharmacology</topic><topic>Cell Line</topic><topic>Cell Proliferation</topic><topic>co-delivery</topic><topic>Cost control</topic><topic>Drug Carriers</topic><topic>Drug Liberation</topic><topic>electrospun nanofibers</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - metabolism</topic><topic>Gene expression</topic><topic>Humans</topic><topic>Infections</topic><topic>Injuries</topic><topic>Keratinocytes - cytology</topic><topic>Keratinocytes - metabolism</topic><topic>Lipopeptides - chemistry</topic><topic>Lipopeptides - pharmacology</topic><topic>Nanofibers - chemistry</topic><topic>pam3Cys-Ser-(Lys)4 peptide</topic><topic>Particle Size</topic><topic>Peptides</topic><topic>Proteins</topic><topic>Silver</topic><topic>Skin - metabolism</topic><topic>Surface Properties</topic><topic>surgical site infection</topic><topic>Surgical site infections</topic><topic>Sutures</topic><topic>Vitamin D</topic><topic>Vitamin deficiency</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Shixuan</creatorcontrib><creatorcontrib>Ge, Liangpeng</creatorcontrib><creatorcontrib>Gombart, Adrian F</creatorcontrib><creatorcontrib>Shuler, Franklin D</creatorcontrib><creatorcontrib>Carlson, Mark A</creatorcontrib><creatorcontrib>Reilly, Debra A</creatorcontrib><creatorcontrib>Xie, Jingwei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>UK & Ireland Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nanomedicine (London, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Shixuan</au><au>Ge, Liangpeng</au><au>Gombart, Adrian F</au><au>Shuler, Franklin D</au><au>Carlson, Mark A</au><au>Reilly, Debra A</au><au>Xie, Jingwei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nanofiber-based sutures induce endogenous antimicrobial peptide</atitle><jtitle>Nanomedicine (London, England)</jtitle><addtitle>Nanomedicine (Lond)</addtitle><date>2017-11-01</date><risdate>2017</risdate><volume>12</volume><issue>21</issue><spage>2597</spage><epage>2609</epage><pages>2597-2609</pages><issn>1743-5889</issn><eissn>1748-6963</eissn><abstract>The aim of this study was to develop nanofiber-based sutures capable of inducing endogenous antimicrobial peptide production.
We used co-axial electrospinning deposition and rolling to fabricate sutures containing pam3CSK4 peptide and 25-hydroxyvitamin D
(25D
).
The diameters and mechanical properties of the sutures were adjustable to meet the criteria of United States Pharmacopeia designation. 25D
exhibited a sustained release from nanofiber sutures over 4 weeks. Pam3CSK4 peptide also showed an initial burst followed by a sustained release over 4 weeks. The co-delivery of 25D
and pam3CSK4 peptide enhanced cathelicidin antimicrobial peptide production from U937 cells and keratinocytes compared with 25D
delivery alone. In addition, the 25D
/pam3CSK4 peptide co-loaded nanofiber sutures did not significantly influence proliferation of keratinocytes, fibroblasts, or the monocytic cell lines U937 and HL-60.
The use of 25D
/pam3CSK4 peptide co-loaded nanofiber sutures could potentially induce endogenous antimicrobial peptide production and reduce surgical site infections.</abstract><cop>England</cop><pub>Future Medicine Ltd</pub><pmid>28960168</pmid><doi>10.2217/nnm-2017-0161</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1743-5889 |
| ispartof | Nanomedicine (London, England), 2017-11, Vol.12 (21), p.2597-2609 |
| issn | 1743-5889 1748-6963 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5674207 |
| source | MEDLINE; PubMed Central |
| subjects | 25-hydroxyvitamin D Antibiotics Antimicrobial agents Antimicrobial Cationic Peptides - biosynthesis Biomechanical Phenomena Calcifediol - chemistry Calcifediol - pharmacology Cell Line Cell Proliferation co-delivery Cost control Drug Carriers Drug Liberation electrospun nanofibers Fibroblasts - cytology Fibroblasts - metabolism Gene expression Humans Infections Injuries Keratinocytes - cytology Keratinocytes - metabolism Lipopeptides - chemistry Lipopeptides - pharmacology Nanofibers - chemistry pam3Cys-Ser-(Lys)4 peptide Particle Size Peptides Proteins Silver Skin - metabolism Surface Properties surgical site infection Surgical site infections Sutures Vitamin D Vitamin deficiency |
| title | Nanofiber-based sutures induce endogenous antimicrobial peptide |
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