Wnt5a induces ROR1 to complex with HS1 to enhance migration of chronic lymphocytic leukemia cells
ROR1 (receptor tyrosine kinase-like orphan receptor 1) is a conserved, oncoembryonic surface antigen expressed in chronic lymphocytic leukemia (CLL). We found that ROR1 associates with hematopoietic-lineage-cell-specific protein 1 (HS1) in freshly isolated CLL cells or in CLL cells cultured with exo...
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| creator | Hasan, M K Yu, J Chen, L Cui, Bing Widhopf II, G F Rassenti, L Shen, Z Briggs, S P Kipps, T J |
| description | ROR1 (receptor tyrosine kinase-like orphan receptor 1) is a conserved, oncoembryonic surface antigen expressed in chronic lymphocytic leukemia (CLL). We found that ROR1 associates with hematopoietic-lineage-cell-specific protein 1 (HS1) in freshly isolated CLL cells or in CLL cells cultured with exogenous Wnt5a. Wnt5a also induced HS1 tyrosine phosphorylation, recruitment of ARHGEF1, activation of RhoA and enhanced chemokine-directed migration; such effects could be inhibited by cirmtuzumab, a humanized anti-ROR1 mAb. We generated truncated forms of ROR1 and found its extracellular cysteine-rich domain or kringle domain was necessary for Wnt5a-induced HS1 phosphorylation. Moreover, the cytoplamic, and more specifically the proline-rich domain (PRD), of ROR1 was required for it to associate with HS1 and allow for F-actin polymerization in response to Wnt5a. Accordingly, we introduced single amino acid substitutions of proline (P) to alanine (A) in the ROR1 PRD at positions 784, 808, 826, 841 or 850 in potential SH3-binding motifs. In contrast to wild-type ROR1, or other ROR1
P→A
mutants, ROR1
P(841)A
had impaired capacity to recruit HS1 and ARHGEF1 to ROR1 in response to Wnt5a. Moreover, Wnt5a could not induce cells expressing ROR1
P(841)A
to phosphorylate HS1 or activate ARHGEF1, and was unable to enhance CLL-cell motility. Collectively, these studies indicate HS1 plays an important role in ROR1-dependent Wnt5a-enhanced chemokine-directed leukemia-cell migration. |
| doi_str_mv | 10.1038/leu.2017.133 |
| format | Article |
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P→A
mutants, ROR1
P(841)A
had impaired capacity to recruit HS1 and ARHGEF1 to ROR1 in response to Wnt5a. Moreover, Wnt5a could not induce cells expressing ROR1
P(841)A
to phosphorylate HS1 or activate ARHGEF1, and was unable to enhance CLL-cell motility. Collectively, these studies indicate HS1 plays an important role in ROR1-dependent Wnt5a-enhanced chemokine-directed leukemia-cell migration.</description><identifier>ISSN: 0887-6924</identifier><identifier>EISSN: 1476-5551</identifier><identifier>DOI: 10.1038/leu.2017.133</identifier><identifier>PMID: 28465529</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/95 ; 14 ; 14/19 ; 631/45/612/1243 ; 631/67/1990/283/1895 ; 631/80/84 ; 631/80/86 ; 692/420/755 ; 82 ; 82/58 ; Actin ; Alanine ; Amino Acid Sequence ; Amino acids ; Analysis ; Blood Proteins - chemistry ; Blood Proteins - metabolism ; Cancer Research ; Care and treatment ; Cell adhesion & migration ; Cell migration ; Cell Movement - immunology ; Chemokines ; Chemokines - metabolism ; Chronic lymphocytic leukemia ; Critical Care Medicine ; Diagnosis ; Hematology ; Humans ; Intensive ; Internal Medicine ; Leukemia ; Leukemia, Lymphocytic, Chronic, B-Cell - immunology ; Leukemia, Lymphocytic, Chronic, B-Cell - metabolism ; Lymphatic leukemia ; Medicine ; Medicine & Public Health ; Microscopy ; Monoclonal antibodies ; Multiprotein Complexes - metabolism ; Mutants ; Oncology ; Original ; original-article ; Phosphorylation ; Polymerization ; Proline ; Protein Binding ; Protein Interaction Domains and Motifs ; Protein-tyrosine kinase receptors ; Receptor Tyrosine Kinase-like Orphan Receptors - chemistry ; Receptor Tyrosine Kinase-like Orphan Receptors - metabolism ; Rho Guanine Nucleotide Exchange Factors - metabolism ; rhoA GTP-Binding Protein - metabolism ; RhoA protein ; Tumor Cells, Cultured ; Tyrosine ; Wnt protein ; Wnt-5a Protein - metabolism</subject><ispartof>Leukemia, 2017-12, Vol.31 (12), p.2615-2622</ispartof><rights>The Author(s) 2017</rights><rights>COPYRIGHT 2017 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Dec 2017</rights><rights>Copyright © 2017 The Author(s) 2017 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c548t-284174b81a30be5eef854451b5b0578717876b80372f58e0a09e9aa46f98ef493</citedby><cites>FETCH-LOGICAL-c548t-284174b81a30be5eef854451b5b0578717876b80372f58e0a09e9aa46f98ef493</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/leu.2017.133$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/leu.2017.133$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,27903,27904,41467,42536,51298</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28465529$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hasan, M K</creatorcontrib><creatorcontrib>Yu, J</creatorcontrib><creatorcontrib>Chen, L</creatorcontrib><creatorcontrib>Cui, Bing</creatorcontrib><creatorcontrib>Widhopf II, G F</creatorcontrib><creatorcontrib>Rassenti, L</creatorcontrib><creatorcontrib>Shen, Z</creatorcontrib><creatorcontrib>Briggs, S P</creatorcontrib><creatorcontrib>Kipps, T J</creatorcontrib><title>Wnt5a induces ROR1 to complex with HS1 to enhance migration of chronic lymphocytic leukemia cells</title><title>Leukemia</title><addtitle>Leukemia</addtitle><addtitle>Leukemia</addtitle><description>ROR1 (receptor tyrosine kinase-like orphan receptor 1) is a conserved, oncoembryonic surface antigen expressed in chronic lymphocytic leukemia (CLL). We found that ROR1 associates with hematopoietic-lineage-cell-specific protein 1 (HS1) in freshly isolated CLL cells or in CLL cells cultured with exogenous Wnt5a. Wnt5a also induced HS1 tyrosine phosphorylation, recruitment of ARHGEF1, activation of RhoA and enhanced chemokine-directed migration; such effects could be inhibited by cirmtuzumab, a humanized anti-ROR1 mAb. We generated truncated forms of ROR1 and found its extracellular cysteine-rich domain or kringle domain was necessary for Wnt5a-induced HS1 phosphorylation. Moreover, the cytoplamic, and more specifically the proline-rich domain (PRD), of ROR1 was required for it to associate with HS1 and allow for F-actin polymerization in response to Wnt5a. Accordingly, we introduced single amino acid substitutions of proline (P) to alanine (A) in the ROR1 PRD at positions 784, 808, 826, 841 or 850 in potential SH3-binding motifs. In contrast to wild-type ROR1, or other ROR1
P→A
mutants, ROR1
P(841)A
had impaired capacity to recruit HS1 and ARHGEF1 to ROR1 in response to Wnt5a. Moreover, Wnt5a could not induce cells expressing ROR1
P(841)A
to phosphorylate HS1 or activate ARHGEF1, and was unable to enhance CLL-cell motility. Collectively, these studies indicate HS1 plays an important role in ROR1-dependent Wnt5a-enhanced chemokine-directed leukemia-cell migration.</description><subject>13/95</subject><subject>14</subject><subject>14/19</subject><subject>631/45/612/1243</subject><subject>631/67/1990/283/1895</subject><subject>631/80/84</subject><subject>631/80/86</subject><subject>692/420/755</subject><subject>82</subject><subject>82/58</subject><subject>Actin</subject><subject>Alanine</subject><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Analysis</subject><subject>Blood Proteins - chemistry</subject><subject>Blood Proteins - metabolism</subject><subject>Cancer Research</subject><subject>Care and treatment</subject><subject>Cell adhesion & migration</subject><subject>Cell migration</subject><subject>Cell Movement - immunology</subject><subject>Chemokines</subject><subject>Chemokines - metabolism</subject><subject>Chronic lymphocytic leukemia</subject><subject>Critical Care Medicine</subject><subject>Diagnosis</subject><subject>Hematology</subject><subject>Humans</subject><subject>Intensive</subject><subject>Internal Medicine</subject><subject>Leukemia</subject><subject>Leukemia, Lymphocytic, Chronic, B-Cell - immunology</subject><subject>Leukemia, Lymphocytic, Chronic, B-Cell - metabolism</subject><subject>Lymphatic leukemia</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Microscopy</subject><subject>Monoclonal antibodies</subject><subject>Multiprotein Complexes - metabolism</subject><subject>Mutants</subject><subject>Oncology</subject><subject>Original</subject><subject>original-article</subject><subject>Phosphorylation</subject><subject>Polymerization</subject><subject>Proline</subject><subject>Protein Binding</subject><subject>Protein Interaction Domains and Motifs</subject><subject>Protein-tyrosine kinase receptors</subject><subject>Receptor Tyrosine Kinase-like Orphan Receptors - chemistry</subject><subject>Receptor Tyrosine Kinase-like Orphan Receptors - metabolism</subject><subject>Rho Guanine Nucleotide Exchange Factors - metabolism</subject><subject>rhoA GTP-Binding Protein - metabolism</subject><subject>RhoA protein</subject><subject>Tumor Cells, Cultured</subject><subject>Tyrosine</subject><subject>Wnt protein</subject><subject>Wnt-5a Protein - metabolism</subject><issn>0887-6924</issn><issn>1476-5551</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNptkt9rFDEQx4Mo9qy--SwBQXxwz2Q32WRfhFKqFQqFqvgYsrnZ29Rsck12tfffm_VqvZMSQsLMZ77J_EDoJSVLSir53sG0LAkVS1pVj9CCMlEXnHP6GC2IlKKom5IdoWcpXRMyO-un6KiUrOa8bBZIf_cj19j61WQg4avLK4rHgE0YNg5u8S879vj8yx8b-F57A3iw66hHGzwOHTZ9DN4a7LbDpg9mO853mH7AYDU24Fx6jp502iV4cXceo28fz76enhcXl58-n55cFIYzORb5S1SwVlJdkRY4QCc5Y5y2vCVcSEHzrltJKlF2XALRpIFGa1Z3jYSONdUx-rDT3UztACsDfozaqU20g45bFbRVhx5ve7UOPxWvBSGlzAJv7wRiuJkgjWqwaU5BewhTUlQ2rKFciBl9_R96Haboc3qKNqKqmlzf-h-11g6U9V3I75pZVJ1wKnhNGSOZWj5A5bXKNTTBQ2ez_SDgzV5AD9qNfQpumluSDsF3O9DEkFKE7r4YlKh5dlTulJpnR-XZyfir_QLew3-HJQPFDkjZ5dcQ97J-SPA3mzXLjg</recordid><startdate>20171201</startdate><enddate>20171201</enddate><creator>Hasan, M K</creator><creator>Yu, J</creator><creator>Chen, L</creator><creator>Cui, Bing</creator><creator>Widhopf II, G F</creator><creator>Rassenti, L</creator><creator>Shen, Z</creator><creator>Briggs, S P</creator><creator>Kipps, T J</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20171201</creationdate><title>Wnt5a induces ROR1 to complex with HS1 to enhance migration of chronic lymphocytic leukemia cells</title><author>Hasan, M K ; Yu, J ; Chen, L ; Cui, Bing ; Widhopf II, G F ; Rassenti, L ; Shen, Z ; Briggs, S P ; Kipps, T J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c548t-284174b81a30be5eef854451b5b0578717876b80372f58e0a09e9aa46f98ef493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>13/95</topic><topic>14</topic><topic>14/19</topic><topic>631/45/612/1243</topic><topic>631/67/1990/283/1895</topic><topic>631/80/84</topic><topic>631/80/86</topic><topic>692/420/755</topic><topic>82</topic><topic>82/58</topic><topic>Actin</topic><topic>Alanine</topic><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Analysis</topic><topic>Blood Proteins - chemistry</topic><topic>Blood Proteins - metabolism</topic><topic>Cancer Research</topic><topic>Care and treatment</topic><topic>Cell adhesion & migration</topic><topic>Cell migration</topic><topic>Cell Movement - immunology</topic><topic>Chemokines</topic><topic>Chemokines - metabolism</topic><topic>Chronic lymphocytic leukemia</topic><topic>Critical Care Medicine</topic><topic>Diagnosis</topic><topic>Hematology</topic><topic>Humans</topic><topic>Intensive</topic><topic>Internal Medicine</topic><topic>Leukemia</topic><topic>Leukemia, Lymphocytic, Chronic, B-Cell - immunology</topic><topic>Leukemia, Lymphocytic, Chronic, B-Cell - metabolism</topic><topic>Lymphatic leukemia</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Microscopy</topic><topic>Monoclonal antibodies</topic><topic>Multiprotein Complexes - metabolism</topic><topic>Mutants</topic><topic>Oncology</topic><topic>Original</topic><topic>original-article</topic><topic>Phosphorylation</topic><topic>Polymerization</topic><topic>Proline</topic><topic>Protein Binding</topic><topic>Protein Interaction Domains and Motifs</topic><topic>Protein-tyrosine kinase receptors</topic><topic>Receptor Tyrosine Kinase-like Orphan Receptors - chemistry</topic><topic>Receptor Tyrosine Kinase-like Orphan Receptors - metabolism</topic><topic>Rho Guanine Nucleotide Exchange Factors - metabolism</topic><topic>rhoA GTP-Binding Protein - metabolism</topic><topic>RhoA protein</topic><topic>Tumor Cells, Cultured</topic><topic>Tyrosine</topic><topic>Wnt protein</topic><topic>Wnt-5a Protein - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hasan, M K</creatorcontrib><creatorcontrib>Yu, J</creatorcontrib><creatorcontrib>Chen, L</creatorcontrib><creatorcontrib>Cui, Bing</creatorcontrib><creatorcontrib>Widhopf II, G F</creatorcontrib><creatorcontrib>Rassenti, L</creatorcontrib><creatorcontrib>Shen, Z</creatorcontrib><creatorcontrib>Briggs, S P</creatorcontrib><creatorcontrib>Kipps, T J</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nursing & Allied Health Database</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Leukemia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hasan, M K</au><au>Yu, J</au><au>Chen, L</au><au>Cui, Bing</au><au>Widhopf II, G F</au><au>Rassenti, L</au><au>Shen, Z</au><au>Briggs, S P</au><au>Kipps, T J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Wnt5a induces ROR1 to complex with HS1 to enhance migration of chronic lymphocytic leukemia cells</atitle><jtitle>Leukemia</jtitle><stitle>Leukemia</stitle><addtitle>Leukemia</addtitle><date>2017-12-01</date><risdate>2017</risdate><volume>31</volume><issue>12</issue><spage>2615</spage><epage>2622</epage><pages>2615-2622</pages><issn>0887-6924</issn><eissn>1476-5551</eissn><abstract>ROR1 (receptor tyrosine kinase-like orphan receptor 1) is a conserved, oncoembryonic surface antigen expressed in chronic lymphocytic leukemia (CLL). We found that ROR1 associates with hematopoietic-lineage-cell-specific protein 1 (HS1) in freshly isolated CLL cells or in CLL cells cultured with exogenous Wnt5a. Wnt5a also induced HS1 tyrosine phosphorylation, recruitment of ARHGEF1, activation of RhoA and enhanced chemokine-directed migration; such effects could be inhibited by cirmtuzumab, a humanized anti-ROR1 mAb. We generated truncated forms of ROR1 and found its extracellular cysteine-rich domain or kringle domain was necessary for Wnt5a-induced HS1 phosphorylation. Moreover, the cytoplamic, and more specifically the proline-rich domain (PRD), of ROR1 was required for it to associate with HS1 and allow for F-actin polymerization in response to Wnt5a. Accordingly, we introduced single amino acid substitutions of proline (P) to alanine (A) in the ROR1 PRD at positions 784, 808, 826, 841 or 850 in potential SH3-binding motifs. In contrast to wild-type ROR1, or other ROR1
P→A
mutants, ROR1
P(841)A
had impaired capacity to recruit HS1 and ARHGEF1 to ROR1 in response to Wnt5a. Moreover, Wnt5a could not induce cells expressing ROR1
P(841)A
to phosphorylate HS1 or activate ARHGEF1, and was unable to enhance CLL-cell motility. Collectively, these studies indicate HS1 plays an important role in ROR1-dependent Wnt5a-enhanced chemokine-directed leukemia-cell migration.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>28465529</pmid><doi>10.1038/leu.2017.133</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0887-6924 |
| ispartof | Leukemia, 2017-12, Vol.31 (12), p.2615-2622 |
| issn | 0887-6924 1476-5551 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5670028 |
| source | MEDLINE; Springer Nature - Complete Springer Journals; Nature Journals Online |
| subjects | 13/95 14 14/19 631/45/612/1243 631/67/1990/283/1895 631/80/84 631/80/86 692/420/755 82 82/58 Actin Alanine Amino Acid Sequence Amino acids Analysis Blood Proteins - chemistry Blood Proteins - metabolism Cancer Research Care and treatment Cell adhesion & migration Cell migration Cell Movement - immunology Chemokines Chemokines - metabolism Chronic lymphocytic leukemia Critical Care Medicine Diagnosis Hematology Humans Intensive Internal Medicine Leukemia Leukemia, Lymphocytic, Chronic, B-Cell - immunology Leukemia, Lymphocytic, Chronic, B-Cell - metabolism Lymphatic leukemia Medicine Medicine & Public Health Microscopy Monoclonal antibodies Multiprotein Complexes - metabolism Mutants Oncology Original original-article Phosphorylation Polymerization Proline Protein Binding Protein Interaction Domains and Motifs Protein-tyrosine kinase receptors Receptor Tyrosine Kinase-like Orphan Receptors - chemistry Receptor Tyrosine Kinase-like Orphan Receptors - metabolism Rho Guanine Nucleotide Exchange Factors - metabolism rhoA GTP-Binding Protein - metabolism RhoA protein Tumor Cells, Cultured Tyrosine Wnt protein Wnt-5a Protein - metabolism |
| title | Wnt5a induces ROR1 to complex with HS1 to enhance migration of chronic lymphocytic leukemia cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T19%3A04%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Wnt5a%20induces%20ROR1%20to%20complex%20with%20HS1%20to%20enhance%20migration%20of%20chronic%20lymphocytic%20leukemia%20cells&rft.jtitle=Leukemia&rft.au=Hasan,%20M%20K&rft.date=2017-12-01&rft.volume=31&rft.issue=12&rft.spage=2615&rft.epage=2622&rft.pages=2615-2622&rft.issn=0887-6924&rft.eissn=1476-5551&rft_id=info:doi/10.1038/leu.2017.133&rft_dat=%3Cgale_pubme%3EA517561440%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1973398466&rft_id=info:pmid/28465529&rft_galeid=A517561440&rfr_iscdi=true |