Knockdown of Yes-Associated Protein Induces the Apoptosis While Inhibits the Proliferation of Human Periodontal Ligament Stem Cells through Crosstalk between Erk and Bcl-2 Signaling Pathways
The purpose of this study was to provide an insight into the biological effects of knockdown Yes-associated protein (YAP) on the proliferation and apoptosis of human periodontal ligament stem cells (h-PDLSCs). Immunofluorescence and Western blot were used to evaluate Hippo-YAP signaling expression l...
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| Veröffentlicht in: | International journal of medical sciences 2017-01, Vol.14 (12), p.1231-1240 |
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| creator | Wen, Yong Ji, Yawen Zhang, Yunpeng Jiang, Baoqi Tang, Cuizhu Wang, Qi Chen, Xiyan Jia, Linglu Gu, Weiting Xu, Xin |
| description | The purpose of this study was to provide an insight into the biological effects of knockdown Yes-associated protein (YAP) on the proliferation and apoptosis of human periodontal ligament stem cells (h-PDLSCs).
Immunofluorescence and Western blot were used to evaluate Hippo-YAP signaling expression level. Enhanced green fluorescence protein lentiviral vector was constructed to down-regulate YAP in h-PDLSCs. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot were used to detect the interfering efficiency of YAP expression. The proliferation activity was detected by EdU staining. Analysis of apoptosis in h-PDLSCs was done through Annexin V-APC staining, while cell cycle analysis was detected by flow cytometry. Cellular senescence was analyzed by β-galactosidase activity detection. The expression of elements in signaling pathways related with proliferation and apoptosis was detected by Western blot.
YAP was located in nucleus and cytoplasm. After the lentivirus transfection, the expression of YAP mRNA and protein was significantly reduced (P |
| doi_str_mv | 10.7150/ijms.20504 |
| format | Article |
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Immunofluorescence and Western blot were used to evaluate Hippo-YAP signaling expression level. Enhanced green fluorescence protein lentiviral vector was constructed to down-regulate YAP in h-PDLSCs. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot were used to detect the interfering efficiency of YAP expression. The proliferation activity was detected by EdU staining. Analysis of apoptosis in h-PDLSCs was done through Annexin V-APC staining, while cell cycle analysis was detected by flow cytometry. Cellular senescence was analyzed by β-galactosidase activity detection. The expression of elements in signaling pathways related with proliferation and apoptosis was detected by Western blot.
YAP was located in nucleus and cytoplasm. After the lentivirus transfection, the expression of YAP mRNA and protein was significantly reduced (P<0.001). When YAP was knocked down, the proliferation activity of h-PDLSCs was inhibited; the early & late apoptosis rates increased; the proportion of cells in G1 phases increased (P<0.05), while that in G2 and S phase decreased (P<0.05); cellular senescence was accelerated (P<0.01); ERK and its target proteins P-P90RSK and P-MEK were reduced while Bcl-2 family members increased.
Knockdown of YAP inhibits the proliferation activity and induces apoptosis of h-PDLSCs with the involvement of Hippo pathway and has a crosstalk between Erk and Bcl-2 signaling pathways.</description><identifier>ISSN: 1449-1907</identifier><identifier>EISSN: 1449-1907</identifier><identifier>DOI: 10.7150/ijms.20504</identifier><identifier>PMID: 29104479</identifier><language>eng</language><publisher>Australia: Ivyspring International Publisher</publisher><subject>Adaptor Proteins, Signal Transducing - genetics ; Adaptor Proteins, Signal Transducing - metabolism ; Apoptosis ; Cell Nucleus - metabolism ; Cell Proliferation ; Cells, Cultured ; Cytoplasm - metabolism ; Down-Regulation ; Gene Knockdown Techniques ; Humans ; MAP Kinase Signaling System ; Periodontal Ligament - cytology ; Phosphoproteins - genetics ; Phosphoproteins - metabolism ; Protein-Serine-Threonine Kinases - metabolism ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; Research Paper ; RNA, Messenger ; RNA, Small Interfering ; Stem Cells - metabolism ; Transcription Factors ; Transfection</subject><ispartof>International journal of medical sciences, 2017-01, Vol.14 (12), p.1231-1240</ispartof><rights>Ivyspring International Publisher 2017</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-ebe74b633ae27500f55dc28ca5ad40f4434617416f012c12865adb161118b9933</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666556/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5666556/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29104479$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wen, Yong</creatorcontrib><creatorcontrib>Ji, Yawen</creatorcontrib><creatorcontrib>Zhang, Yunpeng</creatorcontrib><creatorcontrib>Jiang, Baoqi</creatorcontrib><creatorcontrib>Tang, Cuizhu</creatorcontrib><creatorcontrib>Wang, Qi</creatorcontrib><creatorcontrib>Chen, Xiyan</creatorcontrib><creatorcontrib>Jia, Linglu</creatorcontrib><creatorcontrib>Gu, Weiting</creatorcontrib><creatorcontrib>Xu, Xin</creatorcontrib><title>Knockdown of Yes-Associated Protein Induces the Apoptosis While Inhibits the Proliferation of Human Periodontal Ligament Stem Cells through Crosstalk between Erk and Bcl-2 Signaling Pathways</title><title>International journal of medical sciences</title><addtitle>Int J Med Sci</addtitle><description>The purpose of this study was to provide an insight into the biological effects of knockdown Yes-associated protein (YAP) on the proliferation and apoptosis of human periodontal ligament stem cells (h-PDLSCs).
Immunofluorescence and Western blot were used to evaluate Hippo-YAP signaling expression level. Enhanced green fluorescence protein lentiviral vector was constructed to down-regulate YAP in h-PDLSCs. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot were used to detect the interfering efficiency of YAP expression. The proliferation activity was detected by EdU staining. Analysis of apoptosis in h-PDLSCs was done through Annexin V-APC staining, while cell cycle analysis was detected by flow cytometry. Cellular senescence was analyzed by β-galactosidase activity detection. The expression of elements in signaling pathways related with proliferation and apoptosis was detected by Western blot.
YAP was located in nucleus and cytoplasm. After the lentivirus transfection, the expression of YAP mRNA and protein was significantly reduced (P<0.001). When YAP was knocked down, the proliferation activity of h-PDLSCs was inhibited; the early & late apoptosis rates increased; the proportion of cells in G1 phases increased (P<0.05), while that in G2 and S phase decreased (P<0.05); cellular senescence was accelerated (P<0.01); ERK and its target proteins P-P90RSK and P-MEK were reduced while Bcl-2 family members increased.
Knockdown of YAP inhibits the proliferation activity and induces apoptosis of h-PDLSCs with the involvement of Hippo pathway and has a crosstalk between Erk and Bcl-2 signaling pathways.</description><subject>Adaptor Proteins, Signal Transducing - genetics</subject><subject>Adaptor Proteins, Signal Transducing - metabolism</subject><subject>Apoptosis</subject><subject>Cell Nucleus - metabolism</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Cytoplasm - metabolism</subject><subject>Down-Regulation</subject><subject>Gene Knockdown Techniques</subject><subject>Humans</subject><subject>MAP Kinase Signaling System</subject><subject>Periodontal Ligament - cytology</subject><subject>Phosphoproteins - genetics</subject><subject>Phosphoproteins - metabolism</subject><subject>Protein-Serine-Threonine Kinases - metabolism</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>Research Paper</subject><subject>RNA, Messenger</subject><subject>RNA, Small Interfering</subject><subject>Stem Cells - metabolism</subject><subject>Transcription Factors</subject><subject>Transfection</subject><issn>1449-1907</issn><issn>1449-1907</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkcFu1DAQhi0EoqVw4QGQjwgpxU5sZ3NBWlaFVqzESgUhTpbjTBJ3HXuxHVZ9OZ4Nb7dU5eSR_m9-z8yP0GtKzmvKyXtzM8XzknDCnqBTylhT0IbUTx_VJ-hFjDeEVGVV0-fopGwoYaxuTtGfL87rbef3Dvse_4RYLGP02qgEHd4En8A4fOW6WUPEaQS83Pld8tFE_GM0FrI2mtako5gbrOkhqGT8neHlPCmHNxCM77xLyuK1GdQELuHrBBNegbWH1uDnYcSr4GPM0Ba3kPYADl-ELVauwx-1LUp8bQanrHED3qg07tVtfIme9cpGeHX_nqHvny6-rS6L9dfPV6vlutCMsVRACzVrRVUpKGtOSM95p8uFVlx1jPSMVUzQmlHRE1pqWi5EFloqKKWLtmmq6gx9OPru5naCTucFgrJyF8ykwq30ysj_FWdGOfjfkgshOBfZ4O29QfC_ZohJTibqvL1y4OcoaSMoqVieLaPvjqg-nCNA__ANJfIQuDwELu8Cz_Cbx4M9oP8Srv4Cc-mrBA</recordid><startdate>20170101</startdate><enddate>20170101</enddate><creator>Wen, Yong</creator><creator>Ji, Yawen</creator><creator>Zhang, Yunpeng</creator><creator>Jiang, Baoqi</creator><creator>Tang, Cuizhu</creator><creator>Wang, Qi</creator><creator>Chen, Xiyan</creator><creator>Jia, Linglu</creator><creator>Gu, Weiting</creator><creator>Xu, Xin</creator><general>Ivyspring International Publisher</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170101</creationdate><title>Knockdown of Yes-Associated Protein Induces the Apoptosis While Inhibits the Proliferation of Human Periodontal Ligament Stem Cells through Crosstalk between Erk and Bcl-2 Signaling Pathways</title><author>Wen, Yong ; Ji, Yawen ; Zhang, Yunpeng ; Jiang, Baoqi ; Tang, Cuizhu ; Wang, Qi ; Chen, Xiyan ; Jia, Linglu ; Gu, Weiting ; Xu, Xin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-ebe74b633ae27500f55dc28ca5ad40f4434617416f012c12865adb161118b9933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adaptor Proteins, Signal Transducing - genetics</topic><topic>Adaptor Proteins, Signal Transducing - metabolism</topic><topic>Apoptosis</topic><topic>Cell Nucleus - metabolism</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Cytoplasm - metabolism</topic><topic>Down-Regulation</topic><topic>Gene Knockdown Techniques</topic><topic>Humans</topic><topic>MAP Kinase Signaling System</topic><topic>Periodontal Ligament - cytology</topic><topic>Phosphoproteins - genetics</topic><topic>Phosphoproteins - metabolism</topic><topic>Protein-Serine-Threonine Kinases - metabolism</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>Research Paper</topic><topic>RNA, Messenger</topic><topic>RNA, Small Interfering</topic><topic>Stem Cells - metabolism</topic><topic>Transcription Factors</topic><topic>Transfection</topic><toplevel>online_resources</toplevel><creatorcontrib>Wen, Yong</creatorcontrib><creatorcontrib>Ji, Yawen</creatorcontrib><creatorcontrib>Zhang, Yunpeng</creatorcontrib><creatorcontrib>Jiang, Baoqi</creatorcontrib><creatorcontrib>Tang, Cuizhu</creatorcontrib><creatorcontrib>Wang, Qi</creatorcontrib><creatorcontrib>Chen, Xiyan</creatorcontrib><creatorcontrib>Jia, Linglu</creatorcontrib><creatorcontrib>Gu, Weiting</creatorcontrib><creatorcontrib>Xu, Xin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of medical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wen, Yong</au><au>Ji, Yawen</au><au>Zhang, Yunpeng</au><au>Jiang, Baoqi</au><au>Tang, Cuizhu</au><au>Wang, Qi</au><au>Chen, Xiyan</au><au>Jia, Linglu</au><au>Gu, Weiting</au><au>Xu, Xin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Knockdown of Yes-Associated Protein Induces the Apoptosis While Inhibits the Proliferation of Human Periodontal Ligament Stem Cells through Crosstalk between Erk and Bcl-2 Signaling Pathways</atitle><jtitle>International journal of medical sciences</jtitle><addtitle>Int J Med Sci</addtitle><date>2017-01-01</date><risdate>2017</risdate><volume>14</volume><issue>12</issue><spage>1231</spage><epage>1240</epage><pages>1231-1240</pages><issn>1449-1907</issn><eissn>1449-1907</eissn><abstract>The purpose of this study was to provide an insight into the biological effects of knockdown Yes-associated protein (YAP) on the proliferation and apoptosis of human periodontal ligament stem cells (h-PDLSCs).
Immunofluorescence and Western blot were used to evaluate Hippo-YAP signaling expression level. Enhanced green fluorescence protein lentiviral vector was constructed to down-regulate YAP in h-PDLSCs. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot were used to detect the interfering efficiency of YAP expression. The proliferation activity was detected by EdU staining. Analysis of apoptosis in h-PDLSCs was done through Annexin V-APC staining, while cell cycle analysis was detected by flow cytometry. Cellular senescence was analyzed by β-galactosidase activity detection. The expression of elements in signaling pathways related with proliferation and apoptosis was detected by Western blot.
YAP was located in nucleus and cytoplasm. After the lentivirus transfection, the expression of YAP mRNA and protein was significantly reduced (P<0.001). When YAP was knocked down, the proliferation activity of h-PDLSCs was inhibited; the early & late apoptosis rates increased; the proportion of cells in G1 phases increased (P<0.05), while that in G2 and S phase decreased (P<0.05); cellular senescence was accelerated (P<0.01); ERK and its target proteins P-P90RSK and P-MEK were reduced while Bcl-2 family members increased.
Knockdown of YAP inhibits the proliferation activity and induces apoptosis of h-PDLSCs with the involvement of Hippo pathway and has a crosstalk between Erk and Bcl-2 signaling pathways.</abstract><cop>Australia</cop><pub>Ivyspring International Publisher</pub><pmid>29104479</pmid><doi>10.7150/ijms.20504</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adaptor Proteins, Signal Transducing - genetics Adaptor Proteins, Signal Transducing - metabolism Apoptosis Cell Nucleus - metabolism Cell Proliferation Cells, Cultured Cytoplasm - metabolism Down-Regulation Gene Knockdown Techniques Humans MAP Kinase Signaling System Periodontal Ligament - cytology Phosphoproteins - genetics Phosphoproteins - metabolism Protein-Serine-Threonine Kinases - metabolism Proto-Oncogene Proteins c-bcl-2 - metabolism Research Paper RNA, Messenger RNA, Small Interfering Stem Cells - metabolism Transcription Factors Transfection |
| title | Knockdown of Yes-Associated Protein Induces the Apoptosis While Inhibits the Proliferation of Human Periodontal Ligament Stem Cells through Crosstalk between Erk and Bcl-2 Signaling Pathways |
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