A Marine Bacterium, Bacillus sp. Isolated from the Sediment Samples of Algoa Bay in South Africa Produces a Polysaccharide-Bioflocculant
Bioflocculants mediate the removal of suspended particles from solution and the efficiency of flocculation is dependent on the characteristics of the flocculant. Apart from the merits of biodegradability and harmlessness, bioflocculants could be viable as industrially relevant flocculants as they ar...
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| Veröffentlicht in: | International journal of environmental research and public health 2017-09, Vol.14 (10), p.1149 |
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| creator | Ntozonke, Ncedo Okaiyeto, Kunle Okoli, Arinze S Olaniran, Ademola O Nwodo, Uchechukwu U Okoh, Anthony I |
| description | Bioflocculants mediate the removal of suspended particles from solution and the efficiency of flocculation is dependent on the characteristics of the flocculant. Apart from the merits of biodegradability and harmlessness, bioflocculants could be viable as industrially relevant flocculants as they are a renewable resource. Additionally, the shortcomings associated with the conventionally used flocculants such as aluminium salts and acrylamide polymers, which include dementia and cancer, highlight more the need to use bioflocculants as an alternative. Consequently, in this study a marine sediment bacterial isolate was screened for bioflocculant production. Basic local alignment search tools (BLAST) analysis of 16S ribosomal deoxyribonucleic acid (rDNA) sequence of the bacterial isolate showed 98% similarity to
MR-R1. The bacteria produced bioflocculant optimally with inoculum size (4%
) (85%), glucose (85.65%) and mixed nitrogen source (urea, ammonium chloride and yeast extract) (75.9%) and the divalent cation (Ca
) (62.3%). Under optimal conditions, a maximum flocculating activity of over 85% was attained after 60 h of cultivation. The purified polysaccharide-bioflocculant flocculated optimally at alkaline pH 12 (81%), in the presence of Mn
(73%) and Ca
(72.8%). The high flocculation activity shown indicates that the bioflocculant may contend favourably as an alternative to the conventionally used flocculants in water treatment. |
| doi_str_mv | 10.3390/ijerph14101149 |
| format | Article |
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MR-R1. The bacteria produced bioflocculant optimally with inoculum size (4%
) (85%), glucose (85.65%) and mixed nitrogen source (urea, ammonium chloride and yeast extract) (75.9%) and the divalent cation (Ca
) (62.3%). Under optimal conditions, a maximum flocculating activity of over 85% was attained after 60 h of cultivation. The purified polysaccharide-bioflocculant flocculated optimally at alkaline pH 12 (81%), in the presence of Mn
(73%) and Ca
(72.8%). The high flocculation activity shown indicates that the bioflocculant may contend favourably as an alternative to the conventionally used flocculants in water treatment.</description><identifier>ISSN: 1660-4601</identifier><identifier>ISSN: 1661-7827</identifier><identifier>EISSN: 1660-4601</identifier><identifier>DOI: 10.3390/ijerph14101149</identifier><identifier>PMID: 28961180</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Acrylamide ; Aluminum ; Ammonium ; Ammonium chloride ; Bacillus ; Bacillus - genetics ; Bacillus - metabolism ; Bacteria ; Bays ; Biodegradability ; Biodegradation ; Calcium ; Cancer ; Chlorides ; Cultivation ; Dementia disorders ; Deoxyribonucleic acid ; DNA ; Flocculants ; Flocculation ; Geologic Sediments - microbiology ; Glycerol ; Inoculum ; Marine sediments ; Metabolites ; Microorganisms ; Nitrogen ; Polyacrylamide ; Polymers ; Polysaccharides - chemistry ; Polysaccharides - metabolism ; Public health ; Salts ; Sediments ; South Africa ; Urea ; Water treatment ; Yeast</subject><ispartof>International journal of environmental research and public health, 2017-09, Vol.14 (10), p.1149</ispartof><rights>Copyright MDPI AG 2017</rights><rights>2017 by the authors. 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-7262ae1886df3b8749bbe82890f71f981f020f37e7eb2394d20b9f597f1a0a173</citedby><cites>FETCH-LOGICAL-c418t-7262ae1886df3b8749bbe82890f71f981f020f37e7eb2394d20b9f597f1a0a173</cites><orcidid>0000-0002-9770-085X ; 0000-0002-7211-714X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5664650/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5664650/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28961180$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ntozonke, Ncedo</creatorcontrib><creatorcontrib>Okaiyeto, Kunle</creatorcontrib><creatorcontrib>Okoli, Arinze S</creatorcontrib><creatorcontrib>Olaniran, Ademola O</creatorcontrib><creatorcontrib>Nwodo, Uchechukwu U</creatorcontrib><creatorcontrib>Okoh, Anthony I</creatorcontrib><title>A Marine Bacterium, Bacillus sp. Isolated from the Sediment Samples of Algoa Bay in South Africa Produces a Polysaccharide-Bioflocculant</title><title>International journal of environmental research and public health</title><addtitle>Int J Environ Res Public Health</addtitle><description>Bioflocculants mediate the removal of suspended particles from solution and the efficiency of flocculation is dependent on the characteristics of the flocculant. Apart from the merits of biodegradability and harmlessness, bioflocculants could be viable as industrially relevant flocculants as they are a renewable resource. Additionally, the shortcomings associated with the conventionally used flocculants such as aluminium salts and acrylamide polymers, which include dementia and cancer, highlight more the need to use bioflocculants as an alternative. Consequently, in this study a marine sediment bacterial isolate was screened for bioflocculant production. Basic local alignment search tools (BLAST) analysis of 16S ribosomal deoxyribonucleic acid (rDNA) sequence of the bacterial isolate showed 98% similarity to
MR-R1. The bacteria produced bioflocculant optimally with inoculum size (4%
) (85%), glucose (85.65%) and mixed nitrogen source (urea, ammonium chloride and yeast extract) (75.9%) and the divalent cation (Ca
) (62.3%). Under optimal conditions, a maximum flocculating activity of over 85% was attained after 60 h of cultivation. The purified polysaccharide-bioflocculant flocculated optimally at alkaline pH 12 (81%), in the presence of Mn
(73%) and Ca
(72.8%). The high flocculation activity shown indicates that the bioflocculant may contend favourably as an alternative to the conventionally used flocculants in water treatment.</description><subject>Acrylamide</subject><subject>Aluminum</subject><subject>Ammonium</subject><subject>Ammonium chloride</subject><subject>Bacillus</subject><subject>Bacillus - genetics</subject><subject>Bacillus - metabolism</subject><subject>Bacteria</subject><subject>Bays</subject><subject>Biodegradability</subject><subject>Biodegradation</subject><subject>Calcium</subject><subject>Cancer</subject><subject>Chlorides</subject><subject>Cultivation</subject><subject>Dementia disorders</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Flocculants</subject><subject>Flocculation</subject><subject>Geologic Sediments - microbiology</subject><subject>Glycerol</subject><subject>Inoculum</subject><subject>Marine sediments</subject><subject>Metabolites</subject><subject>Microorganisms</subject><subject>Nitrogen</subject><subject>Polyacrylamide</subject><subject>Polymers</subject><subject>Polysaccharides - chemistry</subject><subject>Polysaccharides - metabolism</subject><subject>Public health</subject><subject>Salts</subject><subject>Sediments</subject><subject>South Africa</subject><subject>Urea</subject><subject>Water treatment</subject><subject>Yeast</subject><issn>1660-4601</issn><issn>1661-7827</issn><issn>1660-4601</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkU9rFTEUxYMotla3LiXgpgvnmcxkMslGeC3-KVQUnq5DJnPTySMzGZOJ8L6BH9s8WkvrKgfyu-few0HoNSWbppHkvdtDXEbKKKGUySfolHJOKsYJffpAn6AXKe0JaQTj8jk6qYXklApyiv5s8Vcd3Qz4QpsVosvTu6N03ueE07LBVyl4vcKAbQwTXkfAOxjcBPOKd3paPCQcLN76m6DL4AG7Ge9CXke8tdEZjb_HMGRTqCKDPyRtzFg2DlBduGB9MCZ7Pa8v0TOrfYJXd-8Z-vnp44_LL9X1t89Xl9vryjAq1qqrea2BCsEH2_SiY7LvQZQ8xHbUSkEtqYltOuigrxvJhpr00rays1QTTbvmDH249V1yP8FgSo6ovVqim3Q8qKCdevwzu1HdhN-q5ZzxlhSD8zuDGH5lSKuaXDLgSwgIOSkqWVtT2fDjrrf_ofuQ41ziFYq3XHRc1IXa3FImhpQi2PtjKFHHktXjksvAm4cR7vF_rTZ_AeTapGc</recordid><startdate>20170929</startdate><enddate>20170929</enddate><creator>Ntozonke, Ncedo</creator><creator>Okaiyeto, Kunle</creator><creator>Okoli, Arinze S</creator><creator>Olaniran, Ademola O</creator><creator>Nwodo, Uchechukwu U</creator><creator>Okoh, Anthony I</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9770-085X</orcidid><orcidid>https://orcid.org/0000-0002-7211-714X</orcidid></search><sort><creationdate>20170929</creationdate><title>A Marine Bacterium, Bacillus sp. 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Isolated from the Sediment Samples of Algoa Bay in South Africa Produces a Polysaccharide-Bioflocculant</atitle><jtitle>International journal of environmental research and public health</jtitle><addtitle>Int J Environ Res Public Health</addtitle><date>2017-09-29</date><risdate>2017</risdate><volume>14</volume><issue>10</issue><spage>1149</spage><pages>1149-</pages><issn>1660-4601</issn><issn>1661-7827</issn><eissn>1660-4601</eissn><abstract>Bioflocculants mediate the removal of suspended particles from solution and the efficiency of flocculation is dependent on the characteristics of the flocculant. Apart from the merits of biodegradability and harmlessness, bioflocculants could be viable as industrially relevant flocculants as they are a renewable resource. Additionally, the shortcomings associated with the conventionally used flocculants such as aluminium salts and acrylamide polymers, which include dementia and cancer, highlight more the need to use bioflocculants as an alternative. Consequently, in this study a marine sediment bacterial isolate was screened for bioflocculant production. Basic local alignment search tools (BLAST) analysis of 16S ribosomal deoxyribonucleic acid (rDNA) sequence of the bacterial isolate showed 98% similarity to
MR-R1. The bacteria produced bioflocculant optimally with inoculum size (4%
) (85%), glucose (85.65%) and mixed nitrogen source (urea, ammonium chloride and yeast extract) (75.9%) and the divalent cation (Ca
) (62.3%). Under optimal conditions, a maximum flocculating activity of over 85% was attained after 60 h of cultivation. The purified polysaccharide-bioflocculant flocculated optimally at alkaline pH 12 (81%), in the presence of Mn
(73%) and Ca
(72.8%). The high flocculation activity shown indicates that the bioflocculant may contend favourably as an alternative to the conventionally used flocculants in water treatment.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>28961180</pmid><doi>10.3390/ijerph14101149</doi><orcidid>https://orcid.org/0000-0002-9770-085X</orcidid><orcidid>https://orcid.org/0000-0002-7211-714X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acrylamide Aluminum Ammonium Ammonium chloride Bacillus Bacillus - genetics Bacillus - metabolism Bacteria Bays Biodegradability Biodegradation Calcium Cancer Chlorides Cultivation Dementia disorders Deoxyribonucleic acid DNA Flocculants Flocculation Geologic Sediments - microbiology Glycerol Inoculum Marine sediments Metabolites Microorganisms Nitrogen Polyacrylamide Polymers Polysaccharides - chemistry Polysaccharides - metabolism Public health Salts Sediments South Africa Urea Water treatment Yeast |
| title | A Marine Bacterium, Bacillus sp. Isolated from the Sediment Samples of Algoa Bay in South Africa Produces a Polysaccharide-Bioflocculant |
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