Occurrence of Virulence Genes Associated with Human Pathogenic Vibrios Isolated from Two Commercial Dusky Kob (Argyrosmus japonicus) Farms and Kareiga Estuary in the Eastern Cape Province, South Africa
: Seafood-borne infections, often linked to contaminated seafood and water, are of increasing global public health concern. The aim of this study was to evaluate the prevalence of human pathogenic vibrios and their associated virulence genes isolated from fish and water samples from 2 commercial dus...
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| creator | Fri, Justine Ndip, Roland Ndip Njom, Henry Akum Clarke, Anna Maria |
| description | : Seafood-borne
infections, often linked to contaminated seafood and water, are of increasing global public health concern. The aim of this study was to evaluate the prevalence of human pathogenic vibrios and their associated virulence genes isolated from fish and water samples from 2 commercial dusky kob farms and Kareiga estuary, South Africa.
: A total of 200 samples including dusky kob fish (
= 120) and seawater (
= 80) were subjected to
screening on thiosulfate-citrate-bile salts-sucrose agar (TCBS). Presumptive isolates were confirmed and delineated to
,
,
, and
by PCR. Various pathogenic gene markers were screened:
(
and
),
(
and
and
,
). Restriction Fragment Length Polymorphism (RFLP) of the
A gene of
strains was performed to determine the associated biotypes.
: Total
prevalence was 59.4% (606/1020) of which
was the most predominant 193 (31.85%), followed by
74 (12.21%) and
33 (5.45%). No
strain was detected. One of the
strains possessed the
gene 7 (9.46%) while most (91.9%; 68/74)
isolates were of the E-type genotype.
virulence genes detected were
(13.5%),
(10.4%) and
(1.0%). 12.16% (9/74) of
strains exhibited a biotype 3 RFLP pattern.
: This is the first report of potentially pathogenic vibrios from healthy marine fish in the study area, and therefore a public health concern. |
| doi_str_mv | 10.3390/ijerph14101111 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5664612</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1965688422</sourcerecordid><originalsourceid>FETCH-LOGICAL-c418t-1333a66c2794b35612b5c3b5352aeb1cf589cb43a5dfe13013a84e5f10f501343</originalsourceid><addsrcrecordid>eNpdkltv1DAQhSMEoqXwyiMaiZcisSWOL01ekFbL9qJWaiUKr9HEO9l4Sexgx632J_KvcC9ULX6xR_7m-Ix1suw9yw84r_IvZkN-7JhgOUvrRbbLlMpnQuXs5ZPzTvYmhE2e81Ko6nW2U5SVUKoUu9mfC62j92Q1gWvhp_GxvyuOyVKAeQhOG5xoBTdm6uAkDmjhEqfOrckanRoab1yA0-D6O6z1boCrGwcLNwzkU3MP32L4tYUz18D-3K-33oUhBtjg6JJEDJ_gCP0QAO0KztCTWSMswxTRb8FYmDqCJYaJvIUFjgSX3l2b5PEzfHcxmZq33mh8m71qsQ_07mHfy34cLa8WJ7Pzi-PTxfx8pgUrpxnjnKNSujisRMOlYkUjNW8klwVSw3Qry0o3gqNctcR4zjiWgmTL8lamQvC97Ou97hibgVaa7OSxr0dvhmS4dmjq5zfWdPXaXddSKZGeSwL7DwLe_Y4UpnowQVPfoyUXQ80qwYtSsOowoR__QzcuepvGS5SSqixFcSt4cE_p9LPBU_tohuX1bUrq5ylJDR-ejvCI_4sF_wtywbyU</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1965688422</pqid></control><display><type>article</type><title>Occurrence of Virulence Genes Associated with Human Pathogenic Vibrios Isolated from Two Commercial Dusky Kob (Argyrosmus japonicus) Farms and Kareiga Estuary in the Eastern Cape Province, South Africa</title><source>MEDLINE</source><source>PubMed Central Open Access</source><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Fri, Justine ; Ndip, Roland Ndip ; Njom, Henry Akum ; Clarke, Anna Maria</creator><creatorcontrib>Fri, Justine ; Ndip, Roland Ndip ; Njom, Henry Akum ; Clarke, Anna Maria</creatorcontrib><description>: Seafood-borne
infections, often linked to contaminated seafood and water, are of increasing global public health concern. The aim of this study was to evaluate the prevalence of human pathogenic vibrios and their associated virulence genes isolated from fish and water samples from 2 commercial dusky kob farms and Kareiga estuary, South Africa.
: A total of 200 samples including dusky kob fish (
= 120) and seawater (
= 80) were subjected to
screening on thiosulfate-citrate-bile salts-sucrose agar (TCBS). Presumptive isolates were confirmed and delineated to
,
,
, and
by PCR. Various pathogenic gene markers were screened:
(
and
),
(
and
and
,
). Restriction Fragment Length Polymorphism (RFLP) of the
A gene of
strains was performed to determine the associated biotypes.
: Total
prevalence was 59.4% (606/1020) of which
was the most predominant 193 (31.85%), followed by
74 (12.21%) and
33 (5.45%). No
strain was detected. One of the
strains possessed the
gene 7 (9.46%) while most (91.9%; 68/74)
isolates were of the E-type genotype.
virulence genes detected were
(13.5%),
(10.4%) and
(1.0%). 12.16% (9/74) of
strains exhibited a biotype 3 RFLP pattern.
: This is the first report of potentially pathogenic vibrios from healthy marine fish in the study area, and therefore a public health concern.</description><identifier>ISSN: 1660-4601</identifier><identifier>ISSN: 1661-7827</identifier><identifier>EISSN: 1660-4601</identifier><identifier>DOI: 10.3390/ijerph14101111</identifier><identifier>PMID: 28946684</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Agar ; Animals ; Aquaculture ; Argyrosomus japonicus ; Bile salts ; Biotypes ; Chemical analysis ; Citric acid ; Epidemiology ; Estuaries ; Fish ; Gene polymorphism ; Genes ; Genes, Bacterial ; Marine fish ; Pathogens ; Perciformes ; Polymerase Chain Reaction ; Polymorphism ; Public health ; Restriction fragment length polymorphism ; Salts ; Seafood ; Seafood - microbiology ; Seawater ; Seawater - microbiology ; South Africa ; Strains (organisms) ; Sucrose ; Sugar ; Thiosulfate ; Vibrio ; Vibrio - classification ; Vibrio - genetics ; Vibrio parahaemolyticus ; Vibrio vulnificus ; Virulence ; Virulence Factors - classification ; Virulence Factors - genetics ; VvhA gene ; Water analysis ; Water pollution ; Water sampling ; Waterborne diseases</subject><ispartof>International journal of environmental research and public health, 2017-09, Vol.14 (10), p.1111</ispartof><rights>Copyright MDPI AG 2017</rights><rights>2017 by the authors. 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-1333a66c2794b35612b5c3b5352aeb1cf589cb43a5dfe13013a84e5f10f501343</citedby><cites>FETCH-LOGICAL-c418t-1333a66c2794b35612b5c3b5352aeb1cf589cb43a5dfe13013a84e5f10f501343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5664612/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5664612/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28946684$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fri, Justine</creatorcontrib><creatorcontrib>Ndip, Roland Ndip</creatorcontrib><creatorcontrib>Njom, Henry Akum</creatorcontrib><creatorcontrib>Clarke, Anna Maria</creatorcontrib><title>Occurrence of Virulence Genes Associated with Human Pathogenic Vibrios Isolated from Two Commercial Dusky Kob (Argyrosmus japonicus) Farms and Kareiga Estuary in the Eastern Cape Province, South Africa</title><title>International journal of environmental research and public health</title><addtitle>Int J Environ Res Public Health</addtitle><description>: Seafood-borne
infections, often linked to contaminated seafood and water, are of increasing global public health concern. The aim of this study was to evaluate the prevalence of human pathogenic vibrios and their associated virulence genes isolated from fish and water samples from 2 commercial dusky kob farms and Kareiga estuary, South Africa.
: A total of 200 samples including dusky kob fish (
= 120) and seawater (
= 80) were subjected to
screening on thiosulfate-citrate-bile salts-sucrose agar (TCBS). Presumptive isolates were confirmed and delineated to
,
,
, and
by PCR. Various pathogenic gene markers were screened:
(
and
),
(
and
and
,
). Restriction Fragment Length Polymorphism (RFLP) of the
A gene of
strains was performed to determine the associated biotypes.
: Total
prevalence was 59.4% (606/1020) of which
was the most predominant 193 (31.85%), followed by
74 (12.21%) and
33 (5.45%). No
strain was detected. One of the
strains possessed the
gene 7 (9.46%) while most (91.9%; 68/74)
isolates were of the E-type genotype.
virulence genes detected were
(13.5%),
(10.4%) and
(1.0%). 12.16% (9/74) of
strains exhibited a biotype 3 RFLP pattern.
: This is the first report of potentially pathogenic vibrios from healthy marine fish in the study area, and therefore a public health concern.</description><subject>Agar</subject><subject>Animals</subject><subject>Aquaculture</subject><subject>Argyrosomus japonicus</subject><subject>Bile salts</subject><subject>Biotypes</subject><subject>Chemical analysis</subject><subject>Citric acid</subject><subject>Epidemiology</subject><subject>Estuaries</subject><subject>Fish</subject><subject>Gene polymorphism</subject><subject>Genes</subject><subject>Genes, Bacterial</subject><subject>Marine fish</subject><subject>Pathogens</subject><subject>Perciformes</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism</subject><subject>Public health</subject><subject>Restriction fragment length polymorphism</subject><subject>Salts</subject><subject>Seafood</subject><subject>Seafood - microbiology</subject><subject>Seawater</subject><subject>Seawater - microbiology</subject><subject>South Africa</subject><subject>Strains (organisms)</subject><subject>Sucrose</subject><subject>Sugar</subject><subject>Thiosulfate</subject><subject>Vibrio</subject><subject>Vibrio - classification</subject><subject>Vibrio - genetics</subject><subject>Vibrio parahaemolyticus</subject><subject>Vibrio vulnificus</subject><subject>Virulence</subject><subject>Virulence Factors - classification</subject><subject>Virulence Factors - genetics</subject><subject>VvhA gene</subject><subject>Water analysis</subject><subject>Water pollution</subject><subject>Water sampling</subject><subject>Waterborne diseases</subject><issn>1660-4601</issn><issn>1661-7827</issn><issn>1660-4601</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkltv1DAQhSMEoqXwyiMaiZcisSWOL01ekFbL9qJWaiUKr9HEO9l4Sexgx632J_KvcC9ULX6xR_7m-Ix1suw9yw84r_IvZkN-7JhgOUvrRbbLlMpnQuXs5ZPzTvYmhE2e81Ko6nW2U5SVUKoUu9mfC62j92Q1gWvhp_GxvyuOyVKAeQhOG5xoBTdm6uAkDmjhEqfOrckanRoab1yA0-D6O6z1boCrGwcLNwzkU3MP32L4tYUz18D-3K-33oUhBtjg6JJEDJ_gCP0QAO0KztCTWSMswxTRb8FYmDqCJYaJvIUFjgSX3l2b5PEzfHcxmZq33mh8m71qsQ_07mHfy34cLa8WJ7Pzi-PTxfx8pgUrpxnjnKNSujisRMOlYkUjNW8klwVSw3Qry0o3gqNctcR4zjiWgmTL8lamQvC97Ou97hibgVaa7OSxr0dvhmS4dmjq5zfWdPXaXddSKZGeSwL7DwLe_Y4UpnowQVPfoyUXQ80qwYtSsOowoR__QzcuepvGS5SSqixFcSt4cE_p9LPBU_tohuX1bUrq5ylJDR-ejvCI_4sF_wtywbyU</recordid><startdate>20170925</startdate><enddate>20170925</enddate><creator>Fri, Justine</creator><creator>Ndip, Roland Ndip</creator><creator>Njom, Henry Akum</creator><creator>Clarke, Anna Maria</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170925</creationdate><title>Occurrence of Virulence Genes Associated with Human Pathogenic Vibrios Isolated from Two Commercial Dusky Kob (Argyrosmus japonicus) Farms and Kareiga Estuary in the Eastern Cape Province, South Africa</title><author>Fri, Justine ; Ndip, Roland Ndip ; Njom, Henry Akum ; Clarke, Anna Maria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-1333a66c2794b35612b5c3b5352aeb1cf589cb43a5dfe13013a84e5f10f501343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Agar</topic><topic>Animals</topic><topic>Aquaculture</topic><topic>Argyrosomus japonicus</topic><topic>Bile salts</topic><topic>Biotypes</topic><topic>Chemical analysis</topic><topic>Citric acid</topic><topic>Epidemiology</topic><topic>Estuaries</topic><topic>Fish</topic><topic>Gene polymorphism</topic><topic>Genes</topic><topic>Genes, Bacterial</topic><topic>Marine fish</topic><topic>Pathogens</topic><topic>Perciformes</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism</topic><topic>Public health</topic><topic>Restriction fragment length polymorphism</topic><topic>Salts</topic><topic>Seafood</topic><topic>Seafood - microbiology</topic><topic>Seawater</topic><topic>Seawater - microbiology</topic><topic>South Africa</topic><topic>Strains (organisms)</topic><topic>Sucrose</topic><topic>Sugar</topic><topic>Thiosulfate</topic><topic>Vibrio</topic><topic>Vibrio - classification</topic><topic>Vibrio - genetics</topic><topic>Vibrio parahaemolyticus</topic><topic>Vibrio vulnificus</topic><topic>Virulence</topic><topic>Virulence Factors - classification</topic><topic>Virulence Factors - genetics</topic><topic>VvhA gene</topic><topic>Water analysis</topic><topic>Water pollution</topic><topic>Water sampling</topic><topic>Waterborne diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fri, Justine</creatorcontrib><creatorcontrib>Ndip, Roland Ndip</creatorcontrib><creatorcontrib>Njom, Henry Akum</creatorcontrib><creatorcontrib>Clarke, Anna Maria</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of environmental research and public health</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fri, Justine</au><au>Ndip, Roland Ndip</au><au>Njom, Henry Akum</au><au>Clarke, Anna Maria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Occurrence of Virulence Genes Associated with Human Pathogenic Vibrios Isolated from Two Commercial Dusky Kob (Argyrosmus japonicus) Farms and Kareiga Estuary in the Eastern Cape Province, South Africa</atitle><jtitle>International journal of environmental research and public health</jtitle><addtitle>Int J Environ Res Public Health</addtitle><date>2017-09-25</date><risdate>2017</risdate><volume>14</volume><issue>10</issue><spage>1111</spage><pages>1111-</pages><issn>1660-4601</issn><issn>1661-7827</issn><eissn>1660-4601</eissn><abstract>: Seafood-borne
infections, often linked to contaminated seafood and water, are of increasing global public health concern. The aim of this study was to evaluate the prevalence of human pathogenic vibrios and their associated virulence genes isolated from fish and water samples from 2 commercial dusky kob farms and Kareiga estuary, South Africa.
: A total of 200 samples including dusky kob fish (
= 120) and seawater (
= 80) were subjected to
screening on thiosulfate-citrate-bile salts-sucrose agar (TCBS). Presumptive isolates were confirmed and delineated to
,
,
, and
by PCR. Various pathogenic gene markers were screened:
(
and
),
(
and
and
,
). Restriction Fragment Length Polymorphism (RFLP) of the
A gene of
strains was performed to determine the associated biotypes.
: Total
prevalence was 59.4% (606/1020) of which
was the most predominant 193 (31.85%), followed by
74 (12.21%) and
33 (5.45%). No
strain was detected. One of the
strains possessed the
gene 7 (9.46%) while most (91.9%; 68/74)
isolates were of the E-type genotype.
virulence genes detected were
(13.5%),
(10.4%) and
(1.0%). 12.16% (9/74) of
strains exhibited a biotype 3 RFLP pattern.
: This is the first report of potentially pathogenic vibrios from healthy marine fish in the study area, and therefore a public health concern.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>28946684</pmid><doi>10.3390/ijerph14101111</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1660-4601 |
| ispartof | International journal of environmental research and public health, 2017-09, Vol.14 (10), p.1111 |
| issn | 1660-4601 1661-7827 1660-4601 |
| language | eng |
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| source | MEDLINE; PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Agar Animals Aquaculture Argyrosomus japonicus Bile salts Biotypes Chemical analysis Citric acid Epidemiology Estuaries Fish Gene polymorphism Genes Genes, Bacterial Marine fish Pathogens Perciformes Polymerase Chain Reaction Polymorphism Public health Restriction fragment length polymorphism Salts Seafood Seafood - microbiology Seawater Seawater - microbiology South Africa Strains (organisms) Sucrose Sugar Thiosulfate Vibrio Vibrio - classification Vibrio - genetics Vibrio parahaemolyticus Vibrio vulnificus Virulence Virulence Factors - classification Virulence Factors - genetics VvhA gene Water analysis Water pollution Water sampling Waterborne diseases |
| title | Occurrence of Virulence Genes Associated with Human Pathogenic Vibrios Isolated from Two Commercial Dusky Kob (Argyrosmus japonicus) Farms and Kareiga Estuary in the Eastern Cape Province, South Africa |
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