Substitution of Aromatic Residues with Polar Residues in the Active Site Pocket of epi-Isozizaene Synthase Leads to the Generation of New Cyclic Sesquiterpenes

The sesquiterpene cyclase epi-isozizaene synthase (EIZS) catalyzes the cyclization of farnesyl diphosphate to form the tricyclic hydrocarbon precursor of the antibiotic albaflavenone. The hydrophobic active site pocket of EIZS serves as a template as it binds and chaperones the flexible substrate an...

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Veröffentlicht in:	Biochemistry (Easton) 2017-10, Vol.56 (43), p.5798-5811
Hauptverfasser:	Blank, Patrick N, Barrow, Golda H, Chou, Wayne K. W, Duan, Lian, Cane, David E, Christianson, David W
Format:	Artikel
Sprache:	eng
Schlagworte:	active sites Amino Acid Substitution antibiotics aromatic compounds Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism BASIC BIOLOGICAL SCIENCES Carbon-Carbon Lyases - chemistry Carbon-Carbon Lyases - genetics Carbon-Carbon Lyases - metabolism catalysts Catalytic Domain crystal structure Crystallography, X-Ray cyclization genetics hydrocarbons Hydrophobic and Hydrophilic Interactions hydrophobicity hydroxylation INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY Models, Molecular mutagenesis mutants Mutation, Missense peptides and proteins Sesquiterpenes - chemistry Sesquiterpenes - metabolism sesquiterpenoids Streptomyces coelicolor - enzymology Streptomyces coelicolor - genetics trichodiene synthase
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