Development and validation of a sensitive assay for analysis of midazolam, free and conjugated 1-hydroxymidazolam and 4-hydroxymidazolam in pediatric plasma: Application to Pediatric Pharmacokinetic Study

•A quantitative LC-MS/MS assay for midazolam and its metabolites in pediatric plasma.•β-glucuronidase employed to measure total hydroxymidazolam metabolites.•Solid phase extraction with μ-elution plates provided a robust sample cleanup.•Pharmacokinetic profile of midazolam and its metabolites in a c...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2017-11, Vol.1067, p.1-9
Hauptverfasser:	Moorthy, Ganesh S., Jogiraju, Harini, Vedar, Christina, Zuppa, Athena F.
Format:	Artikel
Sprache:	eng
Schlagworte:	1-Hydroxymidazolam 4-Hydroxymidazolam beta-glucuronidase Child children Chromatography, High Pressure Liquid - methods clinical trials Drug Stability Female Glucuronidase hydrolysis Glucuronide metabolites high performance liquid chromatography Humans hydroxylation isomers Limit of Detection Linear Models Male metabolites Midazolam Midazolam - analogs & derivatives Midazolam - blood Midazolam - pharmacokinetics monitoring patients pharmacodynamics pharmacokinetics quality control Reproducibility of Results solid phase extraction tandem mass spectrometry Tandem Mass Spectrometry - methods
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!