Microsymbionts of Phaseolus vulgaris in acid and alkaline soils of Mexico
In order to investigate bean-nodulating rhizobia in different types of soil, 41 nodule isolates from acid and alkaline soils in Mexico were characterized. Based upon the phylogenetic studies of 16S rRNA, atpD, glnII, recA, rpoB, gyrB, nifH and nodC genes, the isolates originating from acid soils wer...
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| Veröffentlicht in: | Systematic and applied microbiology 2014-12, Vol.37 (8), p.605-612 |
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| creator | Verástegui-Valdés, Myrthala M. Zhang, Yu Jing Rivera-Orduña, Flor N. Cheng, Hai-Ping Sui, Xing Hua Wang, En Tao |
| description | In order to investigate bean-nodulating rhizobia in different types of soil, 41 nodule isolates from acid and alkaline soils in Mexico were characterized. Based upon the phylogenetic studies of 16S rRNA, atpD, glnII, recA, rpoB, gyrB, nifH and nodC genes, the isolates originating from acid soils were identified as the phaseoli symbiovar of the Rhizobium leguminosarum-like group and Rhizobium grahamii, whereas the isolates from alkaline soils were defined as Ensifer americanum sv. mediterranense and Rhizobium radiobacter. The isolates of “R. leguminosarum” and E. americanum harbored nodC and nifH genes, but the symbiotic genes were not detected in the four isolates of the other two species. It was the first time that “R. leguminosarum” and E. americanum have been reported as bean-nodulating bacteria in Mexico. The high similarity of symbiotic genes in the Rhizobium and Ensifer populations showed that these genes had the same origin and have diversified recently in different rhizobial species. Phenotypic characterization revealed that the “R. leguminosarum” population was more adapted to the acid and low salinity conditions, while the E. americanum population preferred alkaline conditions. The findings of this study have improved the knowledge of the diversity, geographic distribution and evolution of bean-nodulating rhizobia in Mexico. |
| doi_str_mv | 10.1016/j.syapm.2014.08.005 |
| format | Article |
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Based upon the phylogenetic studies of 16S rRNA, atpD, glnII, recA, rpoB, gyrB, nifH and nodC genes, the isolates originating from acid soils were identified as the phaseoli symbiovar of the Rhizobium leguminosarum-like group and Rhizobium grahamii, whereas the isolates from alkaline soils were defined as Ensifer americanum sv. mediterranense and Rhizobium radiobacter. The isolates of “R. leguminosarum” and E. americanum harbored nodC and nifH genes, but the symbiotic genes were not detected in the four isolates of the other two species. It was the first time that “R. leguminosarum” and E. americanum have been reported as bean-nodulating bacteria in Mexico. The high similarity of symbiotic genes in the Rhizobium and Ensifer populations showed that these genes had the same origin and have diversified recently in different rhizobial species. Phenotypic characterization revealed that the “R. leguminosarum” population was more adapted to the acid and low salinity conditions, while the E. americanum population preferred alkaline conditions. The findings of this study have improved the knowledge of the diversity, geographic distribution and evolution of bean-nodulating rhizobia in Mexico.</description><identifier>ISSN: 0723-2020</identifier><identifier>EISSN: 1618-0984</identifier><identifier>DOI: 10.1016/j.syapm.2014.08.005</identifier><identifier>PMID: 25294010</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>acid soils ; acid tolerance ; Agrobacterium radiobacter ; alkaline soils ; bacteria ; Biogeography ; DNA, Bacterial - analysis ; DNA, Bacterial - genetics ; Ensifer ; genes ; geographical distribution ; Hydrogen-Ion Concentration ; Mexico ; microsymbionts ; Mobility ; Molecular Sequence Data ; Phaseolus ; Phaseolus - microbiology ; Phaseolus vulgaris ; phenotype ; Phylogeny ; Rhizobia ; Rhizobium ; Rhizobium - classification ; Rhizobium - genetics ; Rhizobium - physiology ; ribosomal RNA ; RNA, Ribosomal, 16S - genetics ; Root Nodules, Plant - microbiology ; salinity ; Soil - chemistry ; Soil Microbiology ; Soil pH ; Symbiosis</subject><ispartof>Systematic and applied microbiology, 2014-12, Vol.37 (8), p.605-612</ispartof><rights>2014 Elsevier GmbH</rights><rights>Copyright © 2014 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c698t-c6d21da5949d4a2d3e4a708b42b83d68b5a41baf66cd5d51e0da64a3e0d3f3173</citedby><cites>FETCH-LOGICAL-c698t-c6d21da5949d4a2d3e4a708b42b83d68b5a41baf66cd5d51e0da64a3e0d3f3173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0723202014001258$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25294010$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Verástegui-Valdés, Myrthala M.</creatorcontrib><creatorcontrib>Zhang, Yu Jing</creatorcontrib><creatorcontrib>Rivera-Orduña, Flor N.</creatorcontrib><creatorcontrib>Cheng, Hai-Ping</creatorcontrib><creatorcontrib>Sui, Xing Hua</creatorcontrib><creatorcontrib>Wang, En Tao</creatorcontrib><title>Microsymbionts of Phaseolus vulgaris in acid and alkaline soils of Mexico</title><title>Systematic and applied microbiology</title><addtitle>Syst Appl Microbiol</addtitle><description>In order to investigate bean-nodulating rhizobia in different types of soil, 41 nodule isolates from acid and alkaline soils in Mexico were characterized. Based upon the phylogenetic studies of 16S rRNA, atpD, glnII, recA, rpoB, gyrB, nifH and nodC genes, the isolates originating from acid soils were identified as the phaseoli symbiovar of the Rhizobium leguminosarum-like group and Rhizobium grahamii, whereas the isolates from alkaline soils were defined as Ensifer americanum sv. mediterranense and Rhizobium radiobacter. The isolates of “R. leguminosarum” and E. americanum harbored nodC and nifH genes, but the symbiotic genes were not detected in the four isolates of the other two species. It was the first time that “R. leguminosarum” and E. americanum have been reported as bean-nodulating bacteria in Mexico. The high similarity of symbiotic genes in the Rhizobium and Ensifer populations showed that these genes had the same origin and have diversified recently in different rhizobial species. Phenotypic characterization revealed that the “R. leguminosarum” population was more adapted to the acid and low salinity conditions, while the E. americanum population preferred alkaline conditions. The findings of this study have improved the knowledge of the diversity, geographic distribution and evolution of bean-nodulating rhizobia in Mexico.</description><subject>acid soils</subject><subject>acid tolerance</subject><subject>Agrobacterium radiobacter</subject><subject>alkaline soils</subject><subject>bacteria</subject><subject>Biogeography</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Bacterial - genetics</subject><subject>Ensifer</subject><subject>genes</subject><subject>geographical distribution</subject><subject>Hydrogen-Ion Concentration</subject><subject>Mexico</subject><subject>microsymbionts</subject><subject>Mobility</subject><subject>Molecular Sequence Data</subject><subject>Phaseolus</subject><subject>Phaseolus - microbiology</subject><subject>Phaseolus vulgaris</subject><subject>phenotype</subject><subject>Phylogeny</subject><subject>Rhizobia</subject><subject>Rhizobium</subject><subject>Rhizobium - classification</subject><subject>Rhizobium - genetics</subject><subject>Rhizobium - physiology</subject><subject>ribosomal RNA</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Root Nodules, Plant - microbiology</subject><subject>salinity</subject><subject>Soil - chemistry</subject><subject>Soil Microbiology</subject><subject>Soil pH</subject><subject>Symbiosis</subject><issn>0723-2020</issn><issn>1618-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS0EokvhEyChHLlsGP-NfQAJVUArtYIDnC3HdlovTrzYyar77fF22woucLDnML_37JmH0GsMLQYs3m3asjfbsSWAWQuyBeBP0AoLLNegJHuKVtARuiZA4AS9KGUDFVQCP0cnhBPFAMMKXVwFm1PZj31I01yaNDTfbkzxKS6l2S3x2uRQmjA1xgbXmKme-NPEMPmmpBDvBFf-Ntj0Ej0bTCz-1X09RT8-f_p-dr6-_Prl4uzj5doKJed6O4Kd4Yopxwxx1DPTgewZ6SV1QvbcMNybQQjruOPYgzOCGVorHSju6Cn6cPTdLv3onfXTnE3U2xxGk_c6maD_7kzhRl-nneaCK9KJavD23iCnX4svsx5DsT5GM_m0FE0AgFLS8f-jWBClBMFYVpQe0cM6S_bD448w6ENeeqPv8tKHvDRIXfOqqjd_DvOoeQioAu-PgK8r3QWfdbHBT9a7kL2dtUvhnw_8BqoHqPo</recordid><startdate>20141201</startdate><enddate>20141201</enddate><creator>Verástegui-Valdés, Myrthala M.</creator><creator>Zhang, Yu Jing</creator><creator>Rivera-Orduña, Flor N.</creator><creator>Cheng, Hai-Ping</creator><creator>Sui, Xing Hua</creator><creator>Wang, En Tao</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope></search><sort><creationdate>20141201</creationdate><title>Microsymbionts of Phaseolus vulgaris in acid and alkaline soils of Mexico</title><author>Verástegui-Valdés, Myrthala M. ; Zhang, Yu Jing ; Rivera-Orduña, Flor N. ; Cheng, Hai-Ping ; Sui, Xing Hua ; Wang, En Tao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c698t-c6d21da5949d4a2d3e4a708b42b83d68b5a41baf66cd5d51e0da64a3e0d3f3173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>acid soils</topic><topic>acid tolerance</topic><topic>Agrobacterium radiobacter</topic><topic>alkaline soils</topic><topic>bacteria</topic><topic>Biogeography</topic><topic>DNA, Bacterial - analysis</topic><topic>DNA, Bacterial - genetics</topic><topic>Ensifer</topic><topic>genes</topic><topic>geographical distribution</topic><topic>Hydrogen-Ion Concentration</topic><topic>Mexico</topic><topic>microsymbionts</topic><topic>Mobility</topic><topic>Molecular Sequence Data</topic><topic>Phaseolus</topic><topic>Phaseolus - microbiology</topic><topic>Phaseolus vulgaris</topic><topic>phenotype</topic><topic>Phylogeny</topic><topic>Rhizobia</topic><topic>Rhizobium</topic><topic>Rhizobium - classification</topic><topic>Rhizobium - genetics</topic><topic>Rhizobium - physiology</topic><topic>ribosomal RNA</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Root Nodules, Plant - microbiology</topic><topic>salinity</topic><topic>Soil - chemistry</topic><topic>Soil Microbiology</topic><topic>Soil pH</topic><topic>Symbiosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Verástegui-Valdés, Myrthala M.</creatorcontrib><creatorcontrib>Zhang, Yu Jing</creatorcontrib><creatorcontrib>Rivera-Orduña, Flor N.</creatorcontrib><creatorcontrib>Cheng, Hai-Ping</creatorcontrib><creatorcontrib>Sui, Xing Hua</creatorcontrib><creatorcontrib>Wang, En Tao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Systematic and applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Verástegui-Valdés, Myrthala M.</au><au>Zhang, Yu Jing</au><au>Rivera-Orduña, Flor N.</au><au>Cheng, Hai-Ping</au><au>Sui, Xing Hua</au><au>Wang, En Tao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microsymbionts of Phaseolus vulgaris in acid and alkaline soils of Mexico</atitle><jtitle>Systematic and applied microbiology</jtitle><addtitle>Syst Appl Microbiol</addtitle><date>2014-12-01</date><risdate>2014</risdate><volume>37</volume><issue>8</issue><spage>605</spage><epage>612</epage><pages>605-612</pages><issn>0723-2020</issn><eissn>1618-0984</eissn><abstract>In order to investigate bean-nodulating rhizobia in different types of soil, 41 nodule isolates from acid and alkaline soils in Mexico were characterized. Based upon the phylogenetic studies of 16S rRNA, atpD, glnII, recA, rpoB, gyrB, nifH and nodC genes, the isolates originating from acid soils were identified as the phaseoli symbiovar of the Rhizobium leguminosarum-like group and Rhizobium grahamii, whereas the isolates from alkaline soils were defined as Ensifer americanum sv. mediterranense and Rhizobium radiobacter. The isolates of “R. leguminosarum” and E. americanum harbored nodC and nifH genes, but the symbiotic genes were not detected in the four isolates of the other two species. It was the first time that “R. leguminosarum” and E. americanum have been reported as bean-nodulating bacteria in Mexico. The high similarity of symbiotic genes in the Rhizobium and Ensifer populations showed that these genes had the same origin and have diversified recently in different rhizobial species. Phenotypic characterization revealed that the “R. leguminosarum” population was more adapted to the acid and low salinity conditions, while the E. americanum population preferred alkaline conditions. The findings of this study have improved the knowledge of the diversity, geographic distribution and evolution of bean-nodulating rhizobia in Mexico.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>25294010</pmid><doi>10.1016/j.syapm.2014.08.005</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Systematic and applied microbiology, 2014-12, Vol.37 (8), p.605-612 |
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| subjects | acid soils acid tolerance Agrobacterium radiobacter alkaline soils bacteria Biogeography DNA, Bacterial - analysis DNA, Bacterial - genetics Ensifer genes geographical distribution Hydrogen-Ion Concentration Mexico microsymbionts Mobility Molecular Sequence Data Phaseolus Phaseolus - microbiology Phaseolus vulgaris phenotype Phylogeny Rhizobia Rhizobium Rhizobium - classification Rhizobium - genetics Rhizobium - physiology ribosomal RNA RNA, Ribosomal, 16S - genetics Root Nodules, Plant - microbiology salinity Soil - chemistry Soil Microbiology Soil pH Symbiosis |
| title | Microsymbionts of Phaseolus vulgaris in acid and alkaline soils of Mexico |
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