Fangchinoline inhibits migration and causes apoptosis of human breast cancer MDA-MB-231 cells
In order to improve outcomes after breast cancer treatment, it is essential to understand the mechanisms of action of potential therapeutic agents. The effect of fangchinoline (FAN) on migration and apoptosis of human breast cancer MDA-MB-231 cells and its underlying mechanisms were investigated. MD...
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| Veröffentlicht in: | Oncology letters 2017-11, Vol.14 (5), p.5307-5312 |
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| creator | Wang, Binggao Xing, Zhibo Wang, Fengmei Yuan, Xinyan Zhang, Yanhui |
| description | In order to improve outcomes after breast cancer treatment, it is essential to understand the mechanisms of action of potential therapeutic agents. The effect of fangchinoline (FAN) on migration and apoptosis of human breast cancer MDA-MB-231 cells and its underlying mechanisms were investigated. MDA-MB-231 cells were treated with different concentrations of FAN, growth inhibition rates were measured by MTT assay and morphological changes of apoptotic cells were observed by Hoechst staining. The wound-healing assay was used to determine of the effect of FAN on the migration of MDA-MB-231 cells. ELISA was used to detect the expression of MMP-2 and −9 in MDA-MB-231 cells treated with different concentrations of FAN and western blot analysis was used to quantify expression of NF-κβ and Iκβ proteins in the same cells. Our results showed that FAN significantly inhibited the growth of MDA-MB-231 cells in concentration-dependent manner and it induced MDA-MB-231 cell apoptosis. With the high FAN concentrations and long exposure times, the levels of MMP-2 and −9 decreased and the expression of NF-κβ decreased, while the expression of Iκβ protein increased. Based on these results, the antitumor effects of FAN on breast cancer cells can be explained at least partially by inducing apoptosis and inhibiting the migration of MDA-MB-231 cells. |
| doi_str_mv | 10.3892/ol.2017.6831 |
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The effect of fangchinoline (FAN) on migration and apoptosis of human breast cancer MDA-MB-231 cells and its underlying mechanisms were investigated. MDA-MB-231 cells were treated with different concentrations of FAN, growth inhibition rates were measured by MTT assay and morphological changes of apoptotic cells were observed by Hoechst staining. The wound-healing assay was used to determine of the effect of FAN on the migration of MDA-MB-231 cells. ELISA was used to detect the expression of MMP-2 and −9 in MDA-MB-231 cells treated with different concentrations of FAN and western blot analysis was used to quantify expression of NF-κβ and Iκβ proteins in the same cells. Our results showed that FAN significantly inhibited the growth of MDA-MB-231 cells in concentration-dependent manner and it induced MDA-MB-231 cell apoptosis. With the high FAN concentrations and long exposure times, the levels of MMP-2 and −9 decreased and the expression of NF-κβ decreased, while the expression of Iκβ protein increased. Based on these results, the antitumor effects of FAN on breast cancer cells can be explained at least partially by inducing apoptosis and inhibiting the migration of MDA-MB-231 cells.</description><identifier>ISSN: 1792-1074</identifier><identifier>EISSN: 1792-1082</identifier><identifier>DOI: 10.3892/ol.2017.6831</identifier><identifier>PMID: 29098027</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Angiogenesis ; Apoptosis ; Breast cancer ; Care and treatment ; Cell growth ; cell migration ; Cytoplasm ; Development and progression ; Enzymes ; fangchinoline ; Health aspects ; Magnoliidae ; MDA-MB-231 cells ; Metastasis ; Oncology ; Plant products ; Proteins ; Studies</subject><ispartof>Oncology letters, 2017-11, Vol.14 (5), p.5307-5312</ispartof><rights>Copyright: © Wang et al.</rights><rights>COPYRIGHT 2017 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2017</rights><rights>Copyright: © Wang et al. 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c605t-26becf1be3dc47b9d97013d4d07c3e1b811bdef17fe80244470e0af3f23528ea3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5652195/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5652195/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,886,5572,27926,27927,53793,53795</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29098027$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Binggao</creatorcontrib><creatorcontrib>Xing, Zhibo</creatorcontrib><creatorcontrib>Wang, Fengmei</creatorcontrib><creatorcontrib>Yuan, Xinyan</creatorcontrib><creatorcontrib>Zhang, Yanhui</creatorcontrib><title>Fangchinoline inhibits migration and causes apoptosis of human breast cancer MDA-MB-231 cells</title><title>Oncology letters</title><addtitle>Oncol Lett</addtitle><description>In order to improve outcomes after breast cancer treatment, it is essential to understand the mechanisms of action of potential therapeutic agents. The effect of fangchinoline (FAN) on migration and apoptosis of human breast cancer MDA-MB-231 cells and its underlying mechanisms were investigated. MDA-MB-231 cells were treated with different concentrations of FAN, growth inhibition rates were measured by MTT assay and morphological changes of apoptotic cells were observed by Hoechst staining. The wound-healing assay was used to determine of the effect of FAN on the migration of MDA-MB-231 cells. ELISA was used to detect the expression of MMP-2 and −9 in MDA-MB-231 cells treated with different concentrations of FAN and western blot analysis was used to quantify expression of NF-κβ and Iκβ proteins in the same cells. Our results showed that FAN significantly inhibited the growth of MDA-MB-231 cells in concentration-dependent manner and it induced MDA-MB-231 cell apoptosis. With the high FAN concentrations and long exposure times, the levels of MMP-2 and −9 decreased and the expression of NF-κβ decreased, while the expression of Iκβ protein increased. Based on these results, the antitumor effects of FAN on breast cancer cells can be explained at least partially by inducing apoptosis and inhibiting the migration of MDA-MB-231 cells.</description><subject>Angiogenesis</subject><subject>Apoptosis</subject><subject>Breast cancer</subject><subject>Care and treatment</subject><subject>Cell growth</subject><subject>cell migration</subject><subject>Cytoplasm</subject><subject>Development and progression</subject><subject>Enzymes</subject><subject>fangchinoline</subject><subject>Health aspects</subject><subject>Magnoliidae</subject><subject>MDA-MB-231 cells</subject><subject>Metastasis</subject><subject>Oncology</subject><subject>Plant products</subject><subject>Proteins</subject><subject>Studies</subject><issn>1792-1074</issn><issn>1792-1082</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNptkk1v1DAQhiMEolXpjTOyBEIcmsUfSWxfkJbSAtJWvcARWY4z3rhK7MVOkPj3OGxZugjPwZb9zGvP-C2K5wSvmJD0bRhWFBO-agQjj4pTwiUtCRb08WHNq5PiPKU7nEfdECGap8UJlVgKTPlp8e1a-63pnQ-D84Cc713rpoRGt416csEj7Ttk9JwgIb0Luykkl1CwqJ9H7VEbQacpA95ARDcf1uXN-5IyggwMQ3pWPLF6SHB-P58VX6-vvlx-Kje3Hz9frjelaXA9lbRpwVjSAutMxVvZSY4J66oOc8OAtIKQtgNLuIX86qqqOAasLbOU1VSAZmfFu73ubm5H6Az4KepB7aIbdfypgnbq-MS7Xm3DD1U3NSWyzgJv7gVi-D5DmtTo0lKC9hDmpIhssKQidy2jL_9B78IcfS4vU6KWVSPr5i-11QMo523I95pFVK1ryiQRhJNMrf5D5ehgdCZ4sC7vHyW8fpDQgx6mPoVhXn4qHYMXe9DEkFIEe2gGwWqxjgqDWqyjFutk_MXDBh7gP0bJwKs9kHbZEK4L6cDcbkqc47fOL_NkyEo</recordid><startdate>20171101</startdate><enddate>20171101</enddate><creator>Wang, Binggao</creator><creator>Xing, Zhibo</creator><creator>Wang, Fengmei</creator><creator>Yuan, Xinyan</creator><creator>Zhang, Yanhui</creator><general>D.A. Spandidos</general><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20171101</creationdate><title>Fangchinoline inhibits migration and causes apoptosis of human breast cancer MDA-MB-231 cells</title><author>Wang, Binggao ; Xing, Zhibo ; Wang, Fengmei ; Yuan, Xinyan ; Zhang, Yanhui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c605t-26becf1be3dc47b9d97013d4d07c3e1b811bdef17fe80244470e0af3f23528ea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Angiogenesis</topic><topic>Apoptosis</topic><topic>Breast cancer</topic><topic>Care and treatment</topic><topic>Cell growth</topic><topic>cell migration</topic><topic>Cytoplasm</topic><topic>Development and progression</topic><topic>Enzymes</topic><topic>fangchinoline</topic><topic>Health aspects</topic><topic>Magnoliidae</topic><topic>MDA-MB-231 cells</topic><topic>Metastasis</topic><topic>Oncology</topic><topic>Plant products</topic><topic>Proteins</topic><topic>Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Binggao</creatorcontrib><creatorcontrib>Xing, Zhibo</creatorcontrib><creatorcontrib>Wang, Fengmei</creatorcontrib><creatorcontrib>Yuan, Xinyan</creatorcontrib><creatorcontrib>Zhang, Yanhui</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Oncology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Binggao</au><au>Xing, Zhibo</au><au>Wang, Fengmei</au><au>Yuan, Xinyan</au><au>Zhang, Yanhui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fangchinoline inhibits migration and causes apoptosis of human breast cancer MDA-MB-231 cells</atitle><jtitle>Oncology letters</jtitle><addtitle>Oncol Lett</addtitle><date>2017-11-01</date><risdate>2017</risdate><volume>14</volume><issue>5</issue><spage>5307</spage><epage>5312</epage><pages>5307-5312</pages><issn>1792-1074</issn><eissn>1792-1082</eissn><abstract>In order to improve outcomes after breast cancer treatment, it is essential to understand the mechanisms of action of potential therapeutic agents. The effect of fangchinoline (FAN) on migration and apoptosis of human breast cancer MDA-MB-231 cells and its underlying mechanisms were investigated. MDA-MB-231 cells were treated with different concentrations of FAN, growth inhibition rates were measured by MTT assay and morphological changes of apoptotic cells were observed by Hoechst staining. The wound-healing assay was used to determine of the effect of FAN on the migration of MDA-MB-231 cells. ELISA was used to detect the expression of MMP-2 and −9 in MDA-MB-231 cells treated with different concentrations of FAN and western blot analysis was used to quantify expression of NF-κβ and Iκβ proteins in the same cells. Our results showed that FAN significantly inhibited the growth of MDA-MB-231 cells in concentration-dependent manner and it induced MDA-MB-231 cell apoptosis. With the high FAN concentrations and long exposure times, the levels of MMP-2 and −9 decreased and the expression of NF-κβ decreased, while the expression of Iκβ protein increased. Based on these results, the antitumor effects of FAN on breast cancer cells can be explained at least partially by inducing apoptosis and inhibiting the migration of MDA-MB-231 cells.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>29098027</pmid><doi>10.3892/ol.2017.6831</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Angiogenesis Apoptosis Breast cancer Care and treatment Cell growth cell migration Cytoplasm Development and progression Enzymes fangchinoline Health aspects Magnoliidae MDA-MB-231 cells Metastasis Oncology Plant products Proteins Studies |
| title | Fangchinoline inhibits migration and causes apoptosis of human breast cancer MDA-MB-231 cells |
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