Use of the relative release index for histamine in LAD2 cells to evaluate the potential anaphylactoid effects of drugs
Anaphylactoid reactions are common clinical acute adverse drug reactions that can exacerbate a patient’s condition and produce effects that may become life-threatening. Therefore, it is important to establish a novel method to evaluate drugs for anaphylactoid reactions. In this study, we developed a...
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| Veröffentlicht in: | Scientific reports 2017-10, Vol.7 (1), p.13714-9, Article 13714 |
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| creator | Han, Shengli Lv, Yanni Kong, Liyun Che, Delu Liu, Rui Fu, Jia Cao, Jiao Wang, Jue Wang, Cheng He, Huaizhen Zhang, Tao Dong, Xinzhong He, Langchong |
| description | Anaphylactoid reactions are common clinical acute adverse drug reactions that can exacerbate a patient’s condition and produce effects that may become life-threatening. Therefore, it is important to establish a novel method to evaluate drugs for anaphylactoid reactions. In this study, we developed a sensitive and rapid method to detect histamine release from LAD2 cells using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and constructed a relative release index based on various release curve parameters, including allergen release time and sudden change rate, to evaluate the potential and strength of allergen-induced anaphylactoid reactions. This LAD2 release model was used to evaluate anaphylactoid reactions induced by ciprofloxacin, norfloxacin, lomefloxacin, moxifloxacin, and baicalin. The results positively correlated with those obtained with an Evans blue ear test and negatively correlated with the Ca
2+
influx EC
50
. In summary, the current study established a novel
in vitro
method to analyze the properties of histamine release from LAD2 cells and characterize the sensitization and strength of sensitization of drugs or components that may induce anaphylactoid reactions. |
| doi_str_mv | 10.1038/s41598-017-14224-z |
| format | Article |
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2+
influx EC
50
. In summary, the current study established a novel
in vitro
method to analyze the properties of histamine release from LAD2 cells and characterize the sensitization and strength of sensitization of drugs or components that may induce anaphylactoid reactions.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-017-14224-z</identifier><identifier>PMID: 29057927</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/51 ; 631/250 ; 692/699 ; 82 ; 82/16 ; 82/58 ; 96 ; Allergens ; Anaphylactoid reactions ; Anaphylaxis - chemically induced ; Anaphylaxis - metabolism ; Animals ; Anti-Infective Agents - adverse effects ; Baicalin ; Calcium - metabolism ; Calcium influx ; Cations, Divalent - metabolism ; Cell Line ; Chromatography, Liquid - methods ; Ciprofloxacin ; Drug development ; Histamine ; Histamine - analysis ; Histamine - metabolism ; Humanities and Social Sciences ; Humans ; Liquid chromatography ; Lomefloxacin ; Male ; Mass spectrometry ; Mass spectroscopy ; Mast Cells - drug effects ; Mast Cells - metabolism ; Mice, Inbred C57BL ; Moxifloxacin ; multidisciplinary ; Nerve Tissue Proteins - genetics ; Nerve Tissue Proteins - metabolism ; Norfloxacin ; Random Allocation ; Receptors, G-Protein-Coupled - genetics ; Receptors, G-Protein-Coupled - metabolism ; Receptors, Neuropeptide - genetics ; Receptors, Neuropeptide - metabolism ; Science ; Science (multidisciplinary) ; Side effects ; Tandem Mass Spectrometry - methods</subject><ispartof>Scientific reports, 2017-10, Vol.7 (1), p.13714-9, Article 13714</ispartof><rights>The Author(s) 2017</rights><rights>2017. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-62b535cd074d4b70ddc186f85ccffeb3374fb78df071ffc5c209411bb90d37c83</citedby><cites>FETCH-LOGICAL-c540t-62b535cd074d4b70ddc186f85ccffeb3374fb78df071ffc5c209411bb90d37c83</cites><orcidid>0000-0003-1887-6484</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5651870/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5651870/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29057927$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Han, Shengli</creatorcontrib><creatorcontrib>Lv, Yanni</creatorcontrib><creatorcontrib>Kong, Liyun</creatorcontrib><creatorcontrib>Che, Delu</creatorcontrib><creatorcontrib>Liu, Rui</creatorcontrib><creatorcontrib>Fu, Jia</creatorcontrib><creatorcontrib>Cao, Jiao</creatorcontrib><creatorcontrib>Wang, Jue</creatorcontrib><creatorcontrib>Wang, Cheng</creatorcontrib><creatorcontrib>He, Huaizhen</creatorcontrib><creatorcontrib>Zhang, Tao</creatorcontrib><creatorcontrib>Dong, Xinzhong</creatorcontrib><creatorcontrib>He, Langchong</creatorcontrib><title>Use of the relative release index for histamine in LAD2 cells to evaluate the potential anaphylactoid effects of drugs</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Anaphylactoid reactions are common clinical acute adverse drug reactions that can exacerbate a patient’s condition and produce effects that may become life-threatening. Therefore, it is important to establish a novel method to evaluate drugs for anaphylactoid reactions. In this study, we developed a sensitive and rapid method to detect histamine release from LAD2 cells using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and constructed a relative release index based on various release curve parameters, including allergen release time and sudden change rate, to evaluate the potential and strength of allergen-induced anaphylactoid reactions. This LAD2 release model was used to evaluate anaphylactoid reactions induced by ciprofloxacin, norfloxacin, lomefloxacin, moxifloxacin, and baicalin. The results positively correlated with those obtained with an Evans blue ear test and negatively correlated with the Ca
2+
influx EC
50
. In summary, the current study established a novel
in vitro
method to analyze the properties of histamine release from LAD2 cells and characterize the sensitization and strength of sensitization of drugs or components that may induce anaphylactoid reactions.</description><subject>13/51</subject><subject>631/250</subject><subject>692/699</subject><subject>82</subject><subject>82/16</subject><subject>82/58</subject><subject>96</subject><subject>Allergens</subject><subject>Anaphylactoid reactions</subject><subject>Anaphylaxis - chemically induced</subject><subject>Anaphylaxis - metabolism</subject><subject>Animals</subject><subject>Anti-Infective Agents - adverse effects</subject><subject>Baicalin</subject><subject>Calcium - metabolism</subject><subject>Calcium influx</subject><subject>Cations, Divalent - metabolism</subject><subject>Cell Line</subject><subject>Chromatography, Liquid - methods</subject><subject>Ciprofloxacin</subject><subject>Drug development</subject><subject>Histamine</subject><subject>Histamine - analysis</subject><subject>Histamine - 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Therefore, it is important to establish a novel method to evaluate drugs for anaphylactoid reactions. In this study, we developed a sensitive and rapid method to detect histamine release from LAD2 cells using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and constructed a relative release index based on various release curve parameters, including allergen release time and sudden change rate, to evaluate the potential and strength of allergen-induced anaphylactoid reactions. This LAD2 release model was used to evaluate anaphylactoid reactions induced by ciprofloxacin, norfloxacin, lomefloxacin, moxifloxacin, and baicalin. The results positively correlated with those obtained with an Evans blue ear test and negatively correlated with the Ca
2+
influx EC
50
. In summary, the current study established a novel
in vitro
method to analyze the properties of histamine release from LAD2 cells and characterize the sensitization and strength of sensitization of drugs or components that may induce anaphylactoid reactions.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>29057927</pmid><doi>10.1038/s41598-017-14224-z</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-1887-6484</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 13/51 631/250 692/699 82 82/16 82/58 96 Allergens Anaphylactoid reactions Anaphylaxis - chemically induced Anaphylaxis - metabolism Animals Anti-Infective Agents - adverse effects Baicalin Calcium - metabolism Calcium influx Cations, Divalent - metabolism Cell Line Chromatography, Liquid - methods Ciprofloxacin Drug development Histamine Histamine - analysis Histamine - metabolism Humanities and Social Sciences Humans Liquid chromatography Lomefloxacin Male Mass spectrometry Mass spectroscopy Mast Cells - drug effects Mast Cells - metabolism Mice, Inbred C57BL Moxifloxacin multidisciplinary Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Norfloxacin Random Allocation Receptors, G-Protein-Coupled - genetics Receptors, G-Protein-Coupled - metabolism Receptors, Neuropeptide - genetics Receptors, Neuropeptide - metabolism Science Science (multidisciplinary) Side effects Tandem Mass Spectrometry - methods |
| title | Use of the relative release index for histamine in LAD2 cells to evaluate the potential anaphylactoid effects of drugs |
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