Transcription factors and stress response gene alterations in human keratinocytes following Solar Simulated Ultra Violet Radiation
Ultraviolet radiation (UVR) from sunlight is the major effector for skin aging and carcinogenesis. However, genes and pathways altered by solar-simulated UVR (ssUVR), a mixture of UVA and UVB, are not well characterized. Here we report global changes in gene expression as well as associated pathways...
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| Veröffentlicht in: | Scientific reports 2017-10, Vol.7 (1), p.13622-13, Article 13622 |
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| creator | Marais, Thomas L. Des Kluz, Thomas Xu, Dazhong Zhang, Xiaoru Gesumaria, Lisa Matsui, Mary S. Costa, Max Sun, Hong |
| description | Ultraviolet radiation (UVR) from sunlight is the major effector for skin aging and carcinogenesis. However, genes and pathways altered by solar-simulated UVR (ssUVR), a mixture of UVA and UVB, are not well characterized. Here we report global changes in gene expression as well as associated pathways and upstream transcription factors in human keratinocytes exposed to ssUVR. Human HaCaT keratinocytes were exposed to either a single dose or 5 repetitive doses of ssUVR. Comprehensive analyses of gene expression profiles as well as functional annotation were performed at 24 hours post irradiation. Our results revealed that ssUVR modulated genes with diverse cellular functions changed in a dose-dependent manner. Gene expression in cells exposed to a single dose of ssUVR differed significantly from those that underwent repetitive exposures. While single ssUVR caused a significant inhibition in genes involved in cell cycle progression, especially G2/M checkpoint and mitotic regulation, repetitive ssUVR led to extensive changes in genes related to cell signaling and metabolism. We have also identified a panel of ssUVR target genes that exhibited persistent changes in gene expression even at 1 week after irradiation. These results revealed a complex network of transcriptional regulators and pathways that orchestrate the cellular response to ssUVR. |
| doi_str_mv | 10.1038/s41598-017-13765-7 |
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Des ; Kluz, Thomas ; Xu, Dazhong ; Zhang, Xiaoru ; Gesumaria, Lisa ; Matsui, Mary S. ; Costa, Max ; Sun, Hong</creator><creatorcontrib>Marais, Thomas L. Des ; Kluz, Thomas ; Xu, Dazhong ; Zhang, Xiaoru ; Gesumaria, Lisa ; Matsui, Mary S. ; Costa, Max ; Sun, Hong</creatorcontrib><description>Ultraviolet radiation (UVR) from sunlight is the major effector for skin aging and carcinogenesis. However, genes and pathways altered by solar-simulated UVR (ssUVR), a mixture of UVA and UVB, are not well characterized. Here we report global changes in gene expression as well as associated pathways and upstream transcription factors in human keratinocytes exposed to ssUVR. Human HaCaT keratinocytes were exposed to either a single dose or 5 repetitive doses of ssUVR. Comprehensive analyses of gene expression profiles as well as functional annotation were performed at 24 hours post irradiation. Our results revealed that ssUVR modulated genes with diverse cellular functions changed in a dose-dependent manner. Gene expression in cells exposed to a single dose of ssUVR differed significantly from those that underwent repetitive exposures. While single ssUVR caused a significant inhibition in genes involved in cell cycle progression, especially G2/M checkpoint and mitotic regulation, repetitive ssUVR led to extensive changes in genes related to cell signaling and metabolism. We have also identified a panel of ssUVR target genes that exhibited persistent changes in gene expression even at 1 week after irradiation. These results revealed a complex network of transcriptional regulators and pathways that orchestrate the cellular response to ssUVR.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-017-13765-7</identifier><identifier>PMID: 29051608</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/106 ; 38/91 ; 631/61/514 ; 631/67/1813 ; Aging ; Carcinogenesis ; Cell cycle ; Cell Line ; Cellular stress response ; G2 Phase Cell Cycle Checkpoints - radiation effects ; Gene expression ; Gene Expression Regulation - drug effects ; Humanities and Social Sciences ; Humans ; Irradiation ; Keratinocytes ; Keratinocytes - metabolism ; Keratinocytes - radiation effects ; M Phase Cell Cycle Checkpoints - radiation effects ; multidisciplinary ; Science ; Science (multidisciplinary) ; Signal Transduction - radiation effects ; Skin ; Transcription factors ; Transcription Factors - metabolism ; Ultraviolet radiation ; Ultraviolet Rays</subject><ispartof>Scientific reports, 2017-10, Vol.7 (1), p.13622-13, Article 13622</ispartof><rights>The Author(s) 2017</rights><rights>2017. 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Human HaCaT keratinocytes were exposed to either a single dose or 5 repetitive doses of ssUVR. Comprehensive analyses of gene expression profiles as well as functional annotation were performed at 24 hours post irradiation. Our results revealed that ssUVR modulated genes with diverse cellular functions changed in a dose-dependent manner. Gene expression in cells exposed to a single dose of ssUVR differed significantly from those that underwent repetitive exposures. While single ssUVR caused a significant inhibition in genes involved in cell cycle progression, especially G2/M checkpoint and mitotic regulation, repetitive ssUVR led to extensive changes in genes related to cell signaling and metabolism. We have also identified a panel of ssUVR target genes that exhibited persistent changes in gene expression even at 1 week after irradiation. 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Des</au><au>Kluz, Thomas</au><au>Xu, Dazhong</au><au>Zhang, Xiaoru</au><au>Gesumaria, Lisa</au><au>Matsui, Mary S.</au><au>Costa, Max</au><au>Sun, Hong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcription factors and stress response gene alterations in human keratinocytes following Solar Simulated Ultra Violet Radiation</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2017-10-19</date><risdate>2017</risdate><volume>7</volume><issue>1</issue><spage>13622</spage><epage>13</epage><pages>13622-13</pages><artnum>13622</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Ultraviolet radiation (UVR) from sunlight is the major effector for skin aging and carcinogenesis. However, genes and pathways altered by solar-simulated UVR (ssUVR), a mixture of UVA and UVB, are not well characterized. Here we report global changes in gene expression as well as associated pathways and upstream transcription factors in human keratinocytes exposed to ssUVR. Human HaCaT keratinocytes were exposed to either a single dose or 5 repetitive doses of ssUVR. Comprehensive analyses of gene expression profiles as well as functional annotation were performed at 24 hours post irradiation. Our results revealed that ssUVR modulated genes with diverse cellular functions changed in a dose-dependent manner. Gene expression in cells exposed to a single dose of ssUVR differed significantly from those that underwent repetitive exposures. While single ssUVR caused a significant inhibition in genes involved in cell cycle progression, especially G2/M checkpoint and mitotic regulation, repetitive ssUVR led to extensive changes in genes related to cell signaling and metabolism. We have also identified a panel of ssUVR target genes that exhibited persistent changes in gene expression even at 1 week after irradiation. These results revealed a complex network of transcriptional regulators and pathways that orchestrate the cellular response to ssUVR.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>29051608</pmid><doi>10.1038/s41598-017-13765-7</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 13/106 38/91 631/61/514 631/67/1813 Aging Carcinogenesis Cell cycle Cell Line Cellular stress response G2 Phase Cell Cycle Checkpoints - radiation effects Gene expression Gene Expression Regulation - drug effects Humanities and Social Sciences Humans Irradiation Keratinocytes Keratinocytes - metabolism Keratinocytes - radiation effects M Phase Cell Cycle Checkpoints - radiation effects multidisciplinary Science Science (multidisciplinary) Signal Transduction - radiation effects Skin Transcription factors Transcription Factors - metabolism Ultraviolet radiation Ultraviolet Rays |
| title | Transcription factors and stress response gene alterations in human keratinocytes following Solar Simulated Ultra Violet Radiation |
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