A mutation-resistant deoxyribozyme OR gate for highly selective detection of viral nucleic acids

Highly selective probes hybridize only to fully complementary DNA or RNA sequences and, therefore, often fail to recognize mutated viral genomes. Here we designed a probe that possesses two seemingly incompatible properties: it tolerates some point mutations in genome, while it remains selective tow...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2017-09, Vol.53 (76), p.10592-10595
Hauptverfasser:	Kamar, Ola, Sun, Sin-Cih, Lin, Chieh-Hua, Chung, Wan-Yu, Lee, Min-Shi, Liao, Yu-Chieh, Kolpashchikov, Dmitry M, Chuang, Min-Chieh
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Sprache:	eng
Schlagworte:	DNA, Catalytic - genetics DNA, Catalytic - metabolism DNA, Viral - analysis DNA, Viral - genetics Enterovirus A, Human - genetics Fluorescent Dyes - chemistry Mutation Optical Imaging
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container_title	Chemical communications (Cambridge, England)
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creator	Kamar, Ola Sun, Sin-Cih Lin, Chieh-Hua Chung, Wan-Yu Lee, Min-Shi Liao, Yu-Chieh Kolpashchikov, Dmitry M Chuang, Min-Chieh
description	Highly selective probes hybridize only to fully complementary DNA or RNA sequences and, therefore, often fail to recognize mutated viral genomes. Here we designed a probe that possesses two seemingly incompatible properties: it tolerates some point mutations in genome, while it remains selective towards others. An OR deoxyribozyme logic gate was designed to fluorescently report the sequences of enterovirus 71 (EV71) covering ∼90% of all known EV71 strains. Importantly, sequences of closely related coxsackieviruses that differed by single nucleotides were reliably differentiated in 7 out of 8 cases.
doi_str_mv	10.1039/c7cc05576e
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source	MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection
subjects	DNA, Catalytic - genetics DNA, Catalytic - metabolism DNA, Viral - analysis DNA, Viral - genetics Enterovirus A, Human - genetics Fluorescent Dyes - chemistry Mutation Optical Imaging
title	A mutation-resistant deoxyribozyme OR gate for highly selective detection of viral nucleic acids
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