Negative pressure wound therapy: Regulating blood flow perfusion and microvessel maturation through microvascular pericytes
Negative pressure wound therapy (NPWT) has been demonstrated to accelerate wound healing by promoting angiogenesis. However, whether blood flow perfusion is regulated by microvessel maturation and pericytes following NPWT remains unclear, as well as the exact association between pericytes and collag...
Gespeichert in:
| Veröffentlicht in: | International journal of molecular medicine 2017-11, Vol.40 (5), p.1415-1425 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1425 |
|---|---|
| container_issue | 5 |
| container_start_page | 1415 |
| container_title | International journal of molecular medicine |
| container_volume | 40 |
| creator | Ma, Zhanjun Li, Zonghuan Shou, Kangquan Jian, Chao Li, Pengcheng Niu, Yahui Qi, Baiwen Yu, Aixi |
| description | Negative pressure wound therapy (NPWT) has been demonstrated to accelerate wound healing by promoting angiogenesis. However, whether blood flow perfusion is regulated by microvessel maturation and pericytes following NPWT remains unclear, as well as the exact association between pericytes and collagen type IV. The aim of this study was to investigate the relevant association between blood flow perfusion and microvessel maturation and pericytes following NPWT, and to further explore the underlying molecular mechanisms. We also aimed to investigate the association between pericytes and collagen type IV. For this purpose, we created a rat model of diabetic wounds and microvascular blood flow perfusion was detected using a laser Doppler blood perfusion imager. The expression levels of angiogenin-1, tyrosine phosphorylation of tyrosine kinase receptor-2 (Tie-2), α-smooth muscle actin (α-SMA) and collagen type IV were detected and analyzed through immunohistochemistry, immunofluorescence, RT-qPCR and western blot analysis. The results revealed that NPWT promoted the overexpression of angiogenin-1, Tie-2, α-SMA and collagen type IV, and significantly increased blood flow perfusion coupled with microvessel maturation in the NPWT group at the later stages (7-10 days) of wound healing. Our results suggested that NPWT can preferentially enhance vessel maturation and increase the number of pericytes, thus regulating blood flow perfusion. On the other hand, pericytes and collagen type IV had a mutual interaction, promoting microvessel maturation. |
| doi_str_mv | 10.3892/ijmm.2017.3131 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5627868</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A523795505</galeid><sourcerecordid>A523795505</sourcerecordid><originalsourceid>FETCH-LOGICAL-c584t-e3f5e4b75754e2ae7989e72c636bc16ab34612ac81d711b21cf46ec81b6490943</originalsourceid><addsrcrecordid>eNptksuL1EAQxoMo7kOvHiXgxUvGfqbTHoRl0VVYFETBW9PpVDI9JOnYncwy-M9bYcdRYelDP-r3fVRXVZa9oGTDK83e-N0wbBihasMpp4-yc6o0LZgQPx7jmRJVcCXLs-wipR0hTApdPc3OWKUJ5ZqdZ78-Q2dnv4d8ipDSEiG_C8vY5PMWop0Ob_Ov0C09ImOX130ITd724S6fILZL8mHMLcKDdzHsUQ99Pth5ichjaN7GsHTbY9gmh0ZxlXp3mCE9y560tk_w_LhfZt8_vP92_bG4_XLz6frqtnCyEnMBvJUgaiWVFMAsKF1pUMyVvKwdLW3NRUmZdRVtFKU1o64VJeC1LoUmWvDL7N2977TUAzQOxjna3kzRDzYeTLDe_B8Z_dZ0YW9kyVRVVmjw6mgQw88F0mx2YYkj5myoxnpTyjT5S3W2B-PHNqCZG3xy5koyrrSURCK1eYDC1QCWKYzQenx_SIA1TClCe0qcErPOgFlnwKwzYNYZQMHLf797wv80HYHX90CasHm-CenErFaFIAWRBRVU8t_b5L3b</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1979111290</pqid></control><display><type>article</type><title>Negative pressure wound therapy: Regulating blood flow perfusion and microvessel maturation through microvascular pericytes</title><source>Spandidos Publications Journals</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Ma, Zhanjun ; Li, Zonghuan ; Shou, Kangquan ; Jian, Chao ; Li, Pengcheng ; Niu, Yahui ; Qi, Baiwen ; Yu, Aixi</creator><creatorcontrib>Ma, Zhanjun ; Li, Zonghuan ; Shou, Kangquan ; Jian, Chao ; Li, Pengcheng ; Niu, Yahui ; Qi, Baiwen ; Yu, Aixi</creatorcontrib><description>Negative pressure wound therapy (NPWT) has been demonstrated to accelerate wound healing by promoting angiogenesis. However, whether blood flow perfusion is regulated by microvessel maturation and pericytes following NPWT remains unclear, as well as the exact association between pericytes and collagen type IV. The aim of this study was to investigate the relevant association between blood flow perfusion and microvessel maturation and pericytes following NPWT, and to further explore the underlying molecular mechanisms. We also aimed to investigate the association between pericytes and collagen type IV. For this purpose, we created a rat model of diabetic wounds and microvascular blood flow perfusion was detected using a laser Doppler blood perfusion imager. The expression levels of angiogenin-1, tyrosine phosphorylation of tyrosine kinase receptor-2 (Tie-2), α-smooth muscle actin (α-SMA) and collagen type IV were detected and analyzed through immunohistochemistry, immunofluorescence, RT-qPCR and western blot analysis. The results revealed that NPWT promoted the overexpression of angiogenin-1, Tie-2, α-SMA and collagen type IV, and significantly increased blood flow perfusion coupled with microvessel maturation in the NPWT group at the later stages (7-10 days) of wound healing. Our results suggested that NPWT can preferentially enhance vessel maturation and increase the number of pericytes, thus regulating blood flow perfusion. On the other hand, pericytes and collagen type IV had a mutual interaction, promoting microvessel maturation.</description><identifier>ISSN: 1107-3756</identifier><identifier>EISSN: 1791-244X</identifier><identifier>DOI: 10.3892/ijmm.2017.3131</identifier><identifier>PMID: 28901392</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Angiotensin I - genetics ; Angiotensin I - metabolism ; Animals ; Biomarkers ; Blood flow ; blood flow perfusion ; Collagen ; collagen type IV ; Diabetes ; Diabetes Mellitus, Experimental ; Fasting ; Female ; Fluorescent Antibody Technique ; Glucose ; Health aspects ; Immunohistochemistry ; Kinases ; Laboratory animals ; Male ; Medical research ; Microvessels - metabolism ; Microvessels - physiology ; Negative-Pressure Wound Therapy ; Perfusion (Physiology) ; pericytes ; Pericytes - physiology ; Phosphorylation ; Pressure ulcers ; Rats ; Receptor, TIE-2 - metabolism ; Regional Blood Flow ; Rodents ; vessel maturation ; Wound healing ; Wound Healing - physiology</subject><ispartof>International journal of molecular medicine, 2017-11, Vol.40 (5), p.1415-1425</ispartof><rights>Copyright: © Ma et al.</rights><rights>COPYRIGHT 2017 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2017</rights><rights>Copyright: © Ma et al. 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c584t-e3f5e4b75754e2ae7989e72c636bc16ab34612ac81d711b21cf46ec81b6490943</citedby><cites>FETCH-LOGICAL-c584t-e3f5e4b75754e2ae7989e72c636bc16ab34612ac81d711b21cf46ec81b6490943</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,5571,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28901392$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ma, Zhanjun</creatorcontrib><creatorcontrib>Li, Zonghuan</creatorcontrib><creatorcontrib>Shou, Kangquan</creatorcontrib><creatorcontrib>Jian, Chao</creatorcontrib><creatorcontrib>Li, Pengcheng</creatorcontrib><creatorcontrib>Niu, Yahui</creatorcontrib><creatorcontrib>Qi, Baiwen</creatorcontrib><creatorcontrib>Yu, Aixi</creatorcontrib><title>Negative pressure wound therapy: Regulating blood flow perfusion and microvessel maturation through microvascular pericytes</title><title>International journal of molecular medicine</title><addtitle>Int J Mol Med</addtitle><description>Negative pressure wound therapy (NPWT) has been demonstrated to accelerate wound healing by promoting angiogenesis. However, whether blood flow perfusion is regulated by microvessel maturation and pericytes following NPWT remains unclear, as well as the exact association between pericytes and collagen type IV. The aim of this study was to investigate the relevant association between blood flow perfusion and microvessel maturation and pericytes following NPWT, and to further explore the underlying molecular mechanisms. We also aimed to investigate the association between pericytes and collagen type IV. For this purpose, we created a rat model of diabetic wounds and microvascular blood flow perfusion was detected using a laser Doppler blood perfusion imager. The expression levels of angiogenin-1, tyrosine phosphorylation of tyrosine kinase receptor-2 (Tie-2), α-smooth muscle actin (α-SMA) and collagen type IV were detected and analyzed through immunohistochemistry, immunofluorescence, RT-qPCR and western blot analysis. The results revealed that NPWT promoted the overexpression of angiogenin-1, Tie-2, α-SMA and collagen type IV, and significantly increased blood flow perfusion coupled with microvessel maturation in the NPWT group at the later stages (7-10 days) of wound healing. Our results suggested that NPWT can preferentially enhance vessel maturation and increase the number of pericytes, thus regulating blood flow perfusion. On the other hand, pericytes and collagen type IV had a mutual interaction, promoting microvessel maturation.</description><subject>Angiotensin I - genetics</subject><subject>Angiotensin I - metabolism</subject><subject>Animals</subject><subject>Biomarkers</subject><subject>Blood flow</subject><subject>blood flow perfusion</subject><subject>Collagen</subject><subject>collagen type IV</subject><subject>Diabetes</subject><subject>Diabetes Mellitus, Experimental</subject><subject>Fasting</subject><subject>Female</subject><subject>Fluorescent Antibody Technique</subject><subject>Glucose</subject><subject>Health aspects</subject><subject>Immunohistochemistry</subject><subject>Kinases</subject><subject>Laboratory animals</subject><subject>Male</subject><subject>Medical research</subject><subject>Microvessels - metabolism</subject><subject>Microvessels - physiology</subject><subject>Negative-Pressure Wound Therapy</subject><subject>Perfusion (Physiology)</subject><subject>pericytes</subject><subject>Pericytes - physiology</subject><subject>Phosphorylation</subject><subject>Pressure ulcers</subject><subject>Rats</subject><subject>Receptor, TIE-2 - metabolism</subject><subject>Regional Blood Flow</subject><subject>Rodents</subject><subject>vessel maturation</subject><subject>Wound healing</subject><subject>Wound Healing - physiology</subject><issn>1107-3756</issn><issn>1791-244X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNptksuL1EAQxoMo7kOvHiXgxUvGfqbTHoRl0VVYFETBW9PpVDI9JOnYncwy-M9bYcdRYelDP-r3fVRXVZa9oGTDK83e-N0wbBihasMpp4-yc6o0LZgQPx7jmRJVcCXLs-wipR0hTApdPc3OWKUJ5ZqdZ78-Q2dnv4d8ipDSEiG_C8vY5PMWop0Ob_Ov0C09ImOX130ITd724S6fILZL8mHMLcKDdzHsUQ99Pth5ichjaN7GsHTbY9gmh0ZxlXp3mCE9y560tk_w_LhfZt8_vP92_bG4_XLz6frqtnCyEnMBvJUgaiWVFMAsKF1pUMyVvKwdLW3NRUmZdRVtFKU1o64VJeC1LoUmWvDL7N2977TUAzQOxjna3kzRDzYeTLDe_B8Z_dZ0YW9kyVRVVmjw6mgQw88F0mx2YYkj5myoxnpTyjT5S3W2B-PHNqCZG3xy5koyrrSURCK1eYDC1QCWKYzQenx_SIA1TClCe0qcErPOgFlnwKwzYNYZQMHLf797wv80HYHX90CasHm-CenErFaFIAWRBRVU8t_b5L3b</recordid><startdate>20171101</startdate><enddate>20171101</enddate><creator>Ma, Zhanjun</creator><creator>Li, Zonghuan</creator><creator>Shou, Kangquan</creator><creator>Jian, Chao</creator><creator>Li, Pengcheng</creator><creator>Niu, Yahui</creator><creator>Qi, Baiwen</creator><creator>Yu, Aixi</creator><general>D.A. Spandidos</general><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope></search><sort><creationdate>20171101</creationdate><title>Negative pressure wound therapy: Regulating blood flow perfusion and microvessel maturation through microvascular pericytes</title><author>Ma, Zhanjun ; Li, Zonghuan ; Shou, Kangquan ; Jian, Chao ; Li, Pengcheng ; Niu, Yahui ; Qi, Baiwen ; Yu, Aixi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c584t-e3f5e4b75754e2ae7989e72c636bc16ab34612ac81d711b21cf46ec81b6490943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Angiotensin I - genetics</topic><topic>Angiotensin I - metabolism</topic><topic>Animals</topic><topic>Biomarkers</topic><topic>Blood flow</topic><topic>blood flow perfusion</topic><topic>Collagen</topic><topic>collagen type IV</topic><topic>Diabetes</topic><topic>Diabetes Mellitus, Experimental</topic><topic>Fasting</topic><topic>Female</topic><topic>Fluorescent Antibody Technique</topic><topic>Glucose</topic><topic>Health aspects</topic><topic>Immunohistochemistry</topic><topic>Kinases</topic><topic>Laboratory animals</topic><topic>Male</topic><topic>Medical research</topic><topic>Microvessels - metabolism</topic><topic>Microvessels - physiology</topic><topic>Negative-Pressure Wound Therapy</topic><topic>Perfusion (Physiology)</topic><topic>pericytes</topic><topic>Pericytes - physiology</topic><topic>Phosphorylation</topic><topic>Pressure ulcers</topic><topic>Rats</topic><topic>Receptor, TIE-2 - metabolism</topic><topic>Regional Blood Flow</topic><topic>Rodents</topic><topic>vessel maturation</topic><topic>Wound healing</topic><topic>Wound Healing - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ma, Zhanjun</creatorcontrib><creatorcontrib>Li, Zonghuan</creatorcontrib><creatorcontrib>Shou, Kangquan</creatorcontrib><creatorcontrib>Jian, Chao</creatorcontrib><creatorcontrib>Li, Pengcheng</creatorcontrib><creatorcontrib>Niu, Yahui</creatorcontrib><creatorcontrib>Qi, Baiwen</creatorcontrib><creatorcontrib>Yu, Aixi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ma, Zhanjun</au><au>Li, Zonghuan</au><au>Shou, Kangquan</au><au>Jian, Chao</au><au>Li, Pengcheng</au><au>Niu, Yahui</au><au>Qi, Baiwen</au><au>Yu, Aixi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Negative pressure wound therapy: Regulating blood flow perfusion and microvessel maturation through microvascular pericytes</atitle><jtitle>International journal of molecular medicine</jtitle><addtitle>Int J Mol Med</addtitle><date>2017-11-01</date><risdate>2017</risdate><volume>40</volume><issue>5</issue><spage>1415</spage><epage>1425</epage><pages>1415-1425</pages><issn>1107-3756</issn><eissn>1791-244X</eissn><abstract>Negative pressure wound therapy (NPWT) has been demonstrated to accelerate wound healing by promoting angiogenesis. However, whether blood flow perfusion is regulated by microvessel maturation and pericytes following NPWT remains unclear, as well as the exact association between pericytes and collagen type IV. The aim of this study was to investigate the relevant association between blood flow perfusion and microvessel maturation and pericytes following NPWT, and to further explore the underlying molecular mechanisms. We also aimed to investigate the association between pericytes and collagen type IV. For this purpose, we created a rat model of diabetic wounds and microvascular blood flow perfusion was detected using a laser Doppler blood perfusion imager. The expression levels of angiogenin-1, tyrosine phosphorylation of tyrosine kinase receptor-2 (Tie-2), α-smooth muscle actin (α-SMA) and collagen type IV were detected and analyzed through immunohistochemistry, immunofluorescence, RT-qPCR and western blot analysis. The results revealed that NPWT promoted the overexpression of angiogenin-1, Tie-2, α-SMA and collagen type IV, and significantly increased blood flow perfusion coupled with microvessel maturation in the NPWT group at the later stages (7-10 days) of wound healing. Our results suggested that NPWT can preferentially enhance vessel maturation and increase the number of pericytes, thus regulating blood flow perfusion. On the other hand, pericytes and collagen type IV had a mutual interaction, promoting microvessel maturation.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>28901392</pmid><doi>10.3892/ijmm.2017.3131</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1107-3756 |
| ispartof | International journal of molecular medicine, 2017-11, Vol.40 (5), p.1415-1425 |
| issn | 1107-3756 1791-244X |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5627868 |
| source | Spandidos Publications Journals; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Angiotensin I - genetics Angiotensin I - metabolism Animals Biomarkers Blood flow blood flow perfusion Collagen collagen type IV Diabetes Diabetes Mellitus, Experimental Fasting Female Fluorescent Antibody Technique Glucose Health aspects Immunohistochemistry Kinases Laboratory animals Male Medical research Microvessels - metabolism Microvessels - physiology Negative-Pressure Wound Therapy Perfusion (Physiology) pericytes Pericytes - physiology Phosphorylation Pressure ulcers Rats Receptor, TIE-2 - metabolism Regional Blood Flow Rodents vessel maturation Wound healing Wound Healing - physiology |
| title | Negative pressure wound therapy: Regulating blood flow perfusion and microvessel maturation through microvascular pericytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T12%3A24%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Negative%20pressure%20wound%20therapy:%20Regulating%20blood%20flow%20perfusion%20and%20microvessel%20maturation%20through%20microvascular%20pericytes&rft.jtitle=International%20journal%20of%20molecular%20medicine&rft.au=Ma,%20Zhanjun&rft.date=2017-11-01&rft.volume=40&rft.issue=5&rft.spage=1415&rft.epage=1425&rft.pages=1415-1425&rft.issn=1107-3756&rft.eissn=1791-244X&rft_id=info:doi/10.3892/ijmm.2017.3131&rft_dat=%3Cgale_pubme%3EA523795505%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1979111290&rft_id=info:pmid/28901392&rft_galeid=A523795505&rfr_iscdi=true |