Purification and characterization of trypsin from Luphiosilurus alexandri pyloric cecum

Trypsin from . was purified using only two purification processes: ammonium sulfate precipitation and anion exchange liquid chromatography in DEAE-Sepharose. Trypsin mass was estimated as 24 kDa through SDS-PAGE, which showed only one band in silver staining. The purified enzyme showed an optimum te...

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Veröffentlicht in:	Biochemistry and biophysics reports 2016-12, Vol.8, p.29-33
Hauptverfasser:	Dos Santos, Claudio Wilian Victor, da Costa Marques, Maria Elizabeth, de Araújo Tenório, Humberto, de Miranda, Edma Carvalho, Vieira Pereira, Hugo Juarez
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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