Fundamental study on a gene transfection methodology for mammalian cells using water-in-oil droplet deformation in a DC electric field

Fundamental study on a gene transfection methodology for mammalian cells using water-in-oil droplet deformation in a DC electric field

We have developed a gene transfection method called water-in-oil droplet electroporation (EP) that uses a dielectric oil and a liquid droplet containing live cells and exogenous DNA. When a cell suspension droplet is placed between a pair of electrodes, an intense DC electric field can induce drople...

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Veröffentlicht in:Biochemistry and biophysics reports 2016-12, Vol.8, p.81-88
Hauptverfasser: Kurita, Hirofumi, Takao, Yasuhiro, Kishikawa, Kenta, Takashima, Kazunori, Numano, Rika, Mizuno, Akira
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container_title Biochemistry and biophysics reports
container_volume 8
creator Kurita, Hirofumi
Takao, Yasuhiro
Kishikawa, Kenta
Takashima, Kazunori
Numano, Rika
Mizuno, Akira
description We have developed a gene transfection method called water-in-oil droplet electroporation (EP) that uses a dielectric oil and a liquid droplet containing live cells and exogenous DNA. When a cell suspension droplet is placed between a pair of electrodes, an intense DC electric field can induce droplet deformation, resulting in an instantaneous short circuit caused by the droplet elongating and contacting the two electrodes simultaneously. Small transient pores are generated in the cell membrane during the short, allowing the introduction of exogenous DNA into the cells. The droplet EP was characterized by varying the following experimental parameters: applied voltage, number of short circuits, type of medium (electric conductivity), concentration of exogenous DNA, and size of the droplet. In addition, the formation of transient pores in the cell membrane during droplet EP and the transfection efficiency were evaluated.
doi_str_mv 10.1016/j.bbrep.2016.08.001
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title Fundamental study on a gene transfection methodology for mammalian cells using water-in-oil droplet deformation in a DC electric field
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