Amino‐acid composition after loop deletion drives domain swapping

Rational engineering of a protein to enable domain swapping requires an understanding of the sequence, structural and energetic factors that favor the domain‐swapped oligomer over the monomer. While it is known that the deletion of loops between β‐strands can promote domain swapping, the spliced seq...

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Veröffentlicht in:Protein science 2017-10, Vol.26 (10), p.1994-2002
Hauptverfasser: Nandwani, Neha, Surana, Parag, Udgaonkar, Jayant B., Das, Ranabir, Gosavi, Shachi
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container_end_page 2002
container_issue 10
container_start_page 1994
container_title Protein science
container_volume 26
creator Nandwani, Neha
Surana, Parag
Udgaonkar, Jayant B.
Das, Ranabir
Gosavi, Shachi
description Rational engineering of a protein to enable domain swapping requires an understanding of the sequence, structural and energetic factors that favor the domain‐swapped oligomer over the monomer. While it is known that the deletion of loops between β‐strands can promote domain swapping, the spliced sequence at the position of the loop deletion is thought to have a minimal role to play in such domain swapping. Here, two loop‐deletion mutants of the non‐domain‐swapping protein monellin, frame‐shifted by a single residue, were designed. Although the spliced sequence in the two mutants differed by only one residue at the site of the deletion, only one of them (YEIKG) promoted domain swapping. The mutant containing the spliced sequence YENKG was entirely monomeric. This new understanding that the domain swapping propensity after loop deletion may depend critically on the chemical composition of the shortened loop will facilitate the rational design of domain swapping. PDB Code(s): 5XFU
doi_str_mv 10.1002/pro.3237
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subjects Amino acids
Amino Acids - chemistry
Amino Acids - genetics
Crystallography, X-Ray
Deletion mutant
domain swapping
hinge loop composition
hinge loop length
Models, Molecular
Protein Domains
Protein engineering
Protein Engineering - methods
Proteins - chemistry
Proteins - genetics
Proteins - metabolism
Recombinant Proteins
Sequence Deletion
single‐chain monellin
X‐ray crystallography
title Amino‐acid composition after loop deletion drives domain swapping
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