Synthesis and application of magnetite dextran-spermine nanoparticles in breast cancer hyperthermia
Cancer treatment has been very challenging in recent decades. One of the most promising cancer treatment methods is hyperthermia, which increases the tumor temperature (41–45 °C). Magnetic nanoparticles have been widely used for selective targeting of cancer cells. In the present study, magnetic dex...
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| Veröffentlicht in: | Progress in biomaterials 2017-09, Vol.6 (3), p.75-84 |
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| creator | Avazzadeh, Reza Vasheghani-Farahani, Ebrahim Soleimani, Masoud Amanpour, Saeid Sadeghi, Mohsen |
| description | Cancer treatment has been very challenging in recent decades. One of the most promising cancer treatment methods is hyperthermia, which increases the tumor temperature (41–45 °C). Magnetic nanoparticles have been widely used for selective targeting of cancer cells. In the present study, magnetic dextran-spermine nanoparticles, conjugated with Anti-HER2 antibody to target breast cancer cells were developed. The magnetic dextran-spermine nanoparticles (DMNPs) were prepared by ionic gelation, followed by conjugation of antibody to them using EDC-NHS method. Then the Prussian blue method was used to estimate the targeting ability and cellular uptake. Cytotoxicity assay by MTT showed that antibody-conjugated MNPs (ADMNPs) have no toxic effect on SKBR3 and human fibroblast cells. Finally, the hyperthermia was applied to show that synthesized ADMNPs, could increase the cancer cells temperature up to 45 °C and kill most of them without affecting normal cells. These observations proved that Anti-HER2 conjugated magnetic dextran-spermine nanoparticles can target and destroy cancer cells and are potentially suitable for cancer treatment. |
| doi_str_mv | 10.1007/s40204-017-0068-8 |
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One of the most promising cancer treatment methods is hyperthermia, which increases the tumor temperature (41–45 °C). Magnetic nanoparticles have been widely used for selective targeting of cancer cells. In the present study, magnetic dextran-spermine nanoparticles, conjugated with Anti-HER2 antibody to target breast cancer cells were developed. The magnetic dextran-spermine nanoparticles (DMNPs) were prepared by ionic gelation, followed by conjugation of antibody to them using EDC-NHS method. Then the Prussian blue method was used to estimate the targeting ability and cellular uptake. Cytotoxicity assay by MTT showed that antibody-conjugated MNPs (ADMNPs) have no toxic effect on SKBR3 and human fibroblast cells. Finally, the hyperthermia was applied to show that synthesized ADMNPs, could increase the cancer cells temperature up to 45 °C and kill most of them without affecting normal cells. These observations proved that Anti-HER2 conjugated magnetic dextran-spermine nanoparticles can target and destroy cancer cells and are potentially suitable for cancer treatment.</description><identifier>ISSN: 2194-0509</identifier><identifier>EISSN: 2194-0517</identifier><identifier>DOI: 10.1007/s40204-017-0068-8</identifier><identifier>PMID: 28624871</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Biomaterials ; Breast cancer ; Cancer ; Cancer therapies ; Chemistry and Materials Science ; Conjugation ; Cytotoxicity ; Dextran ; ErbB-2 protein ; Fever ; Gelation ; Hyperthermia ; Magnetite ; Materials Science ; Nanoparticles ; Original Research ; Spermine ; Toxicity</subject><ispartof>Progress in biomaterials, 2017-09, Vol.6 (3), p.75-84</ispartof><rights>The Author(s) 2017</rights><rights>Progress in Biomaterials is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-a52915c510a1b963c51f2e7f1abf14cce7f21722cf18d473212a2c9e75bcf9033</citedby><cites>FETCH-LOGICAL-c466t-a52915c510a1b963c51f2e7f1abf14cce7f21722cf18d473212a2c9e75bcf9033</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5597569/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5597569/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,883,27907,27908,41471,42540,51302,53774,53776</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28624871$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Avazzadeh, Reza</creatorcontrib><creatorcontrib>Vasheghani-Farahani, Ebrahim</creatorcontrib><creatorcontrib>Soleimani, Masoud</creatorcontrib><creatorcontrib>Amanpour, Saeid</creatorcontrib><creatorcontrib>Sadeghi, Mohsen</creatorcontrib><title>Synthesis and application of magnetite dextran-spermine nanoparticles in breast cancer hyperthermia</title><title>Progress in biomaterials</title><addtitle>Prog Biomater</addtitle><addtitle>Prog Biomater</addtitle><description>Cancer treatment has been very challenging in recent decades. One of the most promising cancer treatment methods is hyperthermia, which increases the tumor temperature (41–45 °C). Magnetic nanoparticles have been widely used for selective targeting of cancer cells. In the present study, magnetic dextran-spermine nanoparticles, conjugated with Anti-HER2 antibody to target breast cancer cells were developed. The magnetic dextran-spermine nanoparticles (DMNPs) were prepared by ionic gelation, followed by conjugation of antibody to them using EDC-NHS method. Then the Prussian blue method was used to estimate the targeting ability and cellular uptake. Cytotoxicity assay by MTT showed that antibody-conjugated MNPs (ADMNPs) have no toxic effect on SKBR3 and human fibroblast cells. Finally, the hyperthermia was applied to show that synthesized ADMNPs, could increase the cancer cells temperature up to 45 °C and kill most of them without affecting normal cells. These observations proved that Anti-HER2 conjugated magnetic dextran-spermine nanoparticles can target and destroy cancer cells and are potentially suitable for cancer treatment.</description><subject>Biomaterials</subject><subject>Breast cancer</subject><subject>Cancer</subject><subject>Cancer therapies</subject><subject>Chemistry and Materials Science</subject><subject>Conjugation</subject><subject>Cytotoxicity</subject><subject>Dextran</subject><subject>ErbB-2 protein</subject><subject>Fever</subject><subject>Gelation</subject><subject>Hyperthermia</subject><subject>Magnetite</subject><subject>Materials Science</subject><subject>Nanoparticles</subject><subject>Original Research</subject><subject>Spermine</subject><subject>Toxicity</subject><issn>2194-0509</issn><issn>2194-0517</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kUFLJDEQhYMoq6g_YC8S8NxrKt3pdC6CiLqCsIfVc6jOVM9EZtJtkhHn329kdNDD5pJH1VevCh5jP0H8AiH0RWqEFE0lQFdCtF3V7bEjCaZUFOj9nRbmkJ2m9CzK042oQf9gh7JrZdNpOGLu7ybkBSWfOIYZx2laeofZj4GPA1_hPFD2mfiM3nLEUKWJ4soH4gHDOGHM3i0pcR94HwlT5g6Do8gXmwIW4wLjCTsYcJno9OM_Zk-3N4_Xv6uHP3f311cPlWvaNleopAHlFAiE3rR1UYMkPQD2AzTOFSlBS-kG6GaNriVIlM6QVr0bjKjrY3a59Z3W_YpmjkI5eWmn6FcYN3ZEb793gl_Y-fhqlTJataYYnH8YxPFlTSnb53EdQ7nZgqk7BaZroFCwpVwcU4o07DaAsO_R2G00tkRj36OxXZk5-3rabuIziALILZBKK8wpfln9X9d_JUycBA</recordid><startdate>20170901</startdate><enddate>20170901</enddate><creator>Avazzadeh, Reza</creator><creator>Vasheghani-Farahani, Ebrahim</creator><creator>Soleimani, Masoud</creator><creator>Amanpour, Saeid</creator><creator>Sadeghi, Mohsen</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>KB.</scope><scope>L6V</scope><scope>LK8</scope><scope>M7P</scope><scope>M7S</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>5PM</scope></search><sort><creationdate>20170901</creationdate><title>Synthesis and application of magnetite dextran-spermine nanoparticles in breast cancer hyperthermia</title><author>Avazzadeh, Reza ; Vasheghani-Farahani, Ebrahim ; Soleimani, Masoud ; Amanpour, Saeid ; Sadeghi, Mohsen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-a52915c510a1b963c51f2e7f1abf14cce7f21722cf18d473212a2c9e75bcf9033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Biomaterials</topic><topic>Breast cancer</topic><topic>Cancer</topic><topic>Cancer therapies</topic><topic>Chemistry and Materials Science</topic><topic>Conjugation</topic><topic>Cytotoxicity</topic><topic>Dextran</topic><topic>ErbB-2 protein</topic><topic>Fever</topic><topic>Gelation</topic><topic>Hyperthermia</topic><topic>Magnetite</topic><topic>Materials Science</topic><topic>Nanoparticles</topic><topic>Original Research</topic><topic>Spermine</topic><topic>Toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Avazzadeh, Reza</creatorcontrib><creatorcontrib>Vasheghani-Farahani, Ebrahim</creatorcontrib><creatorcontrib>Soleimani, Masoud</creatorcontrib><creatorcontrib>Amanpour, Saeid</creatorcontrib><creatorcontrib>Sadeghi, Mohsen</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection (ProQuest)</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Materials Science Database</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Progress in biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Avazzadeh, Reza</au><au>Vasheghani-Farahani, Ebrahim</au><au>Soleimani, Masoud</au><au>Amanpour, Saeid</au><au>Sadeghi, Mohsen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis and application of magnetite dextran-spermine nanoparticles in breast cancer hyperthermia</atitle><jtitle>Progress in biomaterials</jtitle><stitle>Prog Biomater</stitle><addtitle>Prog Biomater</addtitle><date>2017-09-01</date><risdate>2017</risdate><volume>6</volume><issue>3</issue><spage>75</spage><epage>84</epage><pages>75-84</pages><issn>2194-0509</issn><eissn>2194-0517</eissn><abstract>Cancer treatment has been very challenging in recent decades. One of the most promising cancer treatment methods is hyperthermia, which increases the tumor temperature (41–45 °C). Magnetic nanoparticles have been widely used for selective targeting of cancer cells. In the present study, magnetic dextran-spermine nanoparticles, conjugated with Anti-HER2 antibody to target breast cancer cells were developed. The magnetic dextran-spermine nanoparticles (DMNPs) were prepared by ionic gelation, followed by conjugation of antibody to them using EDC-NHS method. Then the Prussian blue method was used to estimate the targeting ability and cellular uptake. Cytotoxicity assay by MTT showed that antibody-conjugated MNPs (ADMNPs) have no toxic effect on SKBR3 and human fibroblast cells. Finally, the hyperthermia was applied to show that synthesized ADMNPs, could increase the cancer cells temperature up to 45 °C and kill most of them without affecting normal cells. 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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Springer Nature - Complete Springer Journals; PubMed Central |
| subjects | Biomaterials Breast cancer Cancer Cancer therapies Chemistry and Materials Science Conjugation Cytotoxicity Dextran ErbB-2 protein Fever Gelation Hyperthermia Magnetite Materials Science Nanoparticles Original Research Spermine Toxicity |
| title | Synthesis and application of magnetite dextran-spermine nanoparticles in breast cancer hyperthermia |
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