Mast cells and angiogenesis in malignant and premalignant oral lesions: An immunohistochemical study
Angiogenesis is a complex event facilitated by angiogenic factors released from neoplastic and host immune cells. Among host immune cells, mast cells (MCs) may have greater significance in tumor progression through angiogenesis. The objectives of the study were to evaluate and correlate mast cell de...
Gespeichert in:
| Veröffentlicht in: | Journal of oral and maxillofacial pathology : JOMFP 2017-05, Vol.21 (2), p.229-238 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 238 |
|---|---|
| container_issue | 2 |
| container_start_page | 229 |
| container_title | Journal of oral and maxillofacial pathology : JOMFP |
| container_volume | 21 |
| creator | Laishram, Daisy Rao, Kavita Devi, H S Uma Priya, N S Smitha, T Sheethal, H S |
| description | Angiogenesis is a complex event facilitated by angiogenic factors released from neoplastic and host immune cells. Among host immune cells, mast cells (MCs) may have greater significance in tumor progression through angiogenesis. The objectives of the study were to evaluate and correlate mast cell density (MCD) and microvessel density (MVD) in normal gingival tissue, leukoplakia with and without dysplasia and oral squamous cell carcinoma (OSCC) cases.
Among eighty selected cases, twenty were of normal gingiva, twenty each of leukoplakia without and with dysplasia and twenty of OSCC. The slides were stained with CD34 and counterstained with 0.1% toluidine blue, followed by quantification of MCD and MVD per high-power field (×40) using Image-Pro Express software.
Chi-square test and correlation coefficient were used for statistical analysis.
A statistically significant difference in the values of MVD and MCD between normal gingival tissue, leukoplakia with and without dysplasia and OSCC (
= 0.000) was observed. MVD and MCD showed a positive correlation between the study groups.
MVD and MCD increased significantly in cases of OSCC as compared to leukoplakia with and without dysplasia and normal gingival tissue. It was concluded that MCs may play a significant role in angiogenesis by releasing pro-angiogenic and angiogenic factors which may in turn favor the progression of premalignant lesion to a malignant one. |
| doi_str_mv | 10.4103/jomfp.JOMFP_111_15 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5596673</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A501344910</galeid><sourcerecordid>A501344910</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4125-f51ba7fcc8f35956ebb746d0b5235260258caf14b097938718adbdc41623dba93</originalsourceid><addsrcrecordid>eNptUstq3DAUFaGlmUz7A1kUQzfdeKqHJUtdBIaQpC0J6SKB7ISsh0eDLU0su5C_j5ykeZQihODec450rg4AhwiuKgTJt23s3W716_Li9LdECElE98ACCcFLIsjNO7CAoiYlxOJmHxyktIWQ8oriD2Afc0EwJHgBzIVKY6Ft16VCBZN362Nrg00-FT4Uvep8G1QYH7q7wb4U4qC6osvAGNL3Yh0K3_dTiBufxqg3tvc699M4mbuP4L1TXbKfns4luD49uTr-UZ5fnv08Xp-XukKYlo6iRtVOa-4IFZTZpqkrZmBDMaGYQUy5Vg5VTfYlCK8RV6YxmcswMY0SZAmOHnV3U9Nbo20Y8xvlbvC9Gu5kVF6-7QS_kW38IykVjNUkC3x9Ehji7WTTKHuf5uGoYOOUJBIVIozzmmXol3-g2zgNIdubURgzQUn9gmpVZ6UPLuZ79Swq1xQiUlUi_-QSrP6DysvMU4zBOp_rbwj4kaCHmNJg3bNHBOWcDfmQDfk6G5n0-fV0nil_w0DuAfFTt-I</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1942269537</pqid></control><display><type>article</type><title>Mast cells and angiogenesis in malignant and premalignant oral lesions: An immunohistochemical study</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>Medknow Open Access Journals</source><source>PubMed Central</source><creator>Laishram, Daisy ; Rao, Kavita ; Devi, H S Uma ; Priya, N S ; Smitha, T ; Sheethal, H S</creator><creatorcontrib>Laishram, Daisy ; Rao, Kavita ; Devi, H S Uma ; Priya, N S ; Smitha, T ; Sheethal, H S</creatorcontrib><description>Angiogenesis is a complex event facilitated by angiogenic factors released from neoplastic and host immune cells. Among host immune cells, mast cells (MCs) may have greater significance in tumor progression through angiogenesis. The objectives of the study were to evaluate and correlate mast cell density (MCD) and microvessel density (MVD) in normal gingival tissue, leukoplakia with and without dysplasia and oral squamous cell carcinoma (OSCC) cases.
Among eighty selected cases, twenty were of normal gingiva, twenty each of leukoplakia without and with dysplasia and twenty of OSCC. The slides were stained with CD34 and counterstained with 0.1% toluidine blue, followed by quantification of MCD and MVD per high-power field (×40) using Image-Pro Express software.
Chi-square test and correlation coefficient were used for statistical analysis.
A statistically significant difference in the values of MVD and MCD between normal gingival tissue, leukoplakia with and without dysplasia and OSCC (
= 0.000) was observed. MVD and MCD showed a positive correlation between the study groups.
MVD and MCD increased significantly in cases of OSCC as compared to leukoplakia with and without dysplasia and normal gingival tissue. It was concluded that MCs may play a significant role in angiogenesis by releasing pro-angiogenic and angiogenic factors which may in turn favor the progression of premalignant lesion to a malignant one.</description><identifier>ISSN: 0973-029X</identifier><identifier>EISSN: 1998-393X</identifier><identifier>DOI: 10.4103/jomfp.JOMFP_111_15</identifier><identifier>PMID: 28932032</identifier><language>eng</language><publisher>India: Medknow Publications and Media Pvt. Ltd</publisher><subject>Angiogenesis ; Antigens ; CD34 antigen ; Cell density ; Dysplasia ; Endothelium ; Gingiva ; Hypotheses ; Immunohistochemistry ; Leukokeratosis ; Mast cells ; Metastasis ; Mouth cancer ; Neovascularization ; Oral cancer ; Oral squamous cell carcinoma ; Original ; Pathology ; Squamous cell carcinoma ; Stains & staining ; Statistical analysis ; Statistics ; Toluidine ; Tumors</subject><ispartof>Journal of oral and maxillofacial pathology : JOMFP, 2017-05, Vol.21 (2), p.229-238</ispartof><rights>COPYRIGHT 2017 Medknow Publications and Media Pvt. Ltd.</rights><rights>Copyright Medknow Publications & Media Pvt. Ltd. May/Aug 2017</rights><rights>Copyright: © 2017 Journal of Oral and Maxillofacial Pathology 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4125-f51ba7fcc8f35956ebb746d0b5235260258caf14b097938718adbdc41623dba93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5596673/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5596673/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28932032$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Laishram, Daisy</creatorcontrib><creatorcontrib>Rao, Kavita</creatorcontrib><creatorcontrib>Devi, H S Uma</creatorcontrib><creatorcontrib>Priya, N S</creatorcontrib><creatorcontrib>Smitha, T</creatorcontrib><creatorcontrib>Sheethal, H S</creatorcontrib><title>Mast cells and angiogenesis in malignant and premalignant oral lesions: An immunohistochemical study</title><title>Journal of oral and maxillofacial pathology : JOMFP</title><addtitle>J Oral Maxillofac Pathol</addtitle><description>Angiogenesis is a complex event facilitated by angiogenic factors released from neoplastic and host immune cells. Among host immune cells, mast cells (MCs) may have greater significance in tumor progression through angiogenesis. The objectives of the study were to evaluate and correlate mast cell density (MCD) and microvessel density (MVD) in normal gingival tissue, leukoplakia with and without dysplasia and oral squamous cell carcinoma (OSCC) cases.
Among eighty selected cases, twenty were of normal gingiva, twenty each of leukoplakia without and with dysplasia and twenty of OSCC. The slides were stained with CD34 and counterstained with 0.1% toluidine blue, followed by quantification of MCD and MVD per high-power field (×40) using Image-Pro Express software.
Chi-square test and correlation coefficient were used for statistical analysis.
A statistically significant difference in the values of MVD and MCD between normal gingival tissue, leukoplakia with and without dysplasia and OSCC (
= 0.000) was observed. MVD and MCD showed a positive correlation between the study groups.
MVD and MCD increased significantly in cases of OSCC as compared to leukoplakia with and without dysplasia and normal gingival tissue. It was concluded that MCs may play a significant role in angiogenesis by releasing pro-angiogenic and angiogenic factors which may in turn favor the progression of premalignant lesion to a malignant one.</description><subject>Angiogenesis</subject><subject>Antigens</subject><subject>CD34 antigen</subject><subject>Cell density</subject><subject>Dysplasia</subject><subject>Endothelium</subject><subject>Gingiva</subject><subject>Hypotheses</subject><subject>Immunohistochemistry</subject><subject>Leukokeratosis</subject><subject>Mast cells</subject><subject>Metastasis</subject><subject>Mouth cancer</subject><subject>Neovascularization</subject><subject>Oral cancer</subject><subject>Oral squamous cell carcinoma</subject><subject>Original</subject><subject>Pathology</subject><subject>Squamous cell carcinoma</subject><subject>Stains & staining</subject><subject>Statistical analysis</subject><subject>Statistics</subject><subject>Toluidine</subject><subject>Tumors</subject><issn>0973-029X</issn><issn>1998-393X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNptUstq3DAUFaGlmUz7A1kUQzfdeKqHJUtdBIaQpC0J6SKB7ISsh0eDLU0su5C_j5ykeZQihODec450rg4AhwiuKgTJt23s3W716_Li9LdECElE98ACCcFLIsjNO7CAoiYlxOJmHxyktIWQ8oriD2Afc0EwJHgBzIVKY6Ft16VCBZN362Nrg00-FT4Uvep8G1QYH7q7wb4U4qC6osvAGNL3Yh0K3_dTiBufxqg3tvc699M4mbuP4L1TXbKfns4luD49uTr-UZ5fnv08Xp-XukKYlo6iRtVOa-4IFZTZpqkrZmBDMaGYQUy5Vg5VTfYlCK8RV6YxmcswMY0SZAmOHnV3U9Nbo20Y8xvlbvC9Gu5kVF6-7QS_kW38IykVjNUkC3x9Ehji7WTTKHuf5uGoYOOUJBIVIozzmmXol3-g2zgNIdubURgzQUn9gmpVZ6UPLuZ79Swq1xQiUlUi_-QSrP6DysvMU4zBOp_rbwj4kaCHmNJg3bNHBOWcDfmQDfk6G5n0-fV0nil_w0DuAfFTt-I</recordid><startdate>20170501</startdate><enddate>20170501</enddate><creator>Laishram, Daisy</creator><creator>Rao, Kavita</creator><creator>Devi, H S Uma</creator><creator>Priya, N S</creator><creator>Smitha, T</creator><creator>Sheethal, H S</creator><general>Medknow Publications and Media Pvt. Ltd</general><general>Medknow Publications & Media Pvt. Ltd</general><general>Medknow Publications & Media Pvt Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170501</creationdate><title>Mast cells and angiogenesis in malignant and premalignant oral lesions: An immunohistochemical study</title><author>Laishram, Daisy ; Rao, Kavita ; Devi, H S Uma ; Priya, N S ; Smitha, T ; Sheethal, H S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4125-f51ba7fcc8f35956ebb746d0b5235260258caf14b097938718adbdc41623dba93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Angiogenesis</topic><topic>Antigens</topic><topic>CD34 antigen</topic><topic>Cell density</topic><topic>Dysplasia</topic><topic>Endothelium</topic><topic>Gingiva</topic><topic>Hypotheses</topic><topic>Immunohistochemistry</topic><topic>Leukokeratosis</topic><topic>Mast cells</topic><topic>Metastasis</topic><topic>Mouth cancer</topic><topic>Neovascularization</topic><topic>Oral cancer</topic><topic>Oral squamous cell carcinoma</topic><topic>Original</topic><topic>Pathology</topic><topic>Squamous cell carcinoma</topic><topic>Stains & staining</topic><topic>Statistical analysis</topic><topic>Statistics</topic><topic>Toluidine</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Laishram, Daisy</creatorcontrib><creatorcontrib>Rao, Kavita</creatorcontrib><creatorcontrib>Devi, H S Uma</creatorcontrib><creatorcontrib>Priya, N S</creatorcontrib><creatorcontrib>Smitha, T</creatorcontrib><creatorcontrib>Sheethal, H S</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of oral and maxillofacial pathology : JOMFP</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Laishram, Daisy</au><au>Rao, Kavita</au><au>Devi, H S Uma</au><au>Priya, N S</au><au>Smitha, T</au><au>Sheethal, H S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mast cells and angiogenesis in malignant and premalignant oral lesions: An immunohistochemical study</atitle><jtitle>Journal of oral and maxillofacial pathology : JOMFP</jtitle><addtitle>J Oral Maxillofac Pathol</addtitle><date>2017-05-01</date><risdate>2017</risdate><volume>21</volume><issue>2</issue><spage>229</spage><epage>238</epage><pages>229-238</pages><issn>0973-029X</issn><eissn>1998-393X</eissn><abstract>Angiogenesis is a complex event facilitated by angiogenic factors released from neoplastic and host immune cells. Among host immune cells, mast cells (MCs) may have greater significance in tumor progression through angiogenesis. The objectives of the study were to evaluate and correlate mast cell density (MCD) and microvessel density (MVD) in normal gingival tissue, leukoplakia with and without dysplasia and oral squamous cell carcinoma (OSCC) cases.
Among eighty selected cases, twenty were of normal gingiva, twenty each of leukoplakia without and with dysplasia and twenty of OSCC. The slides were stained with CD34 and counterstained with 0.1% toluidine blue, followed by quantification of MCD and MVD per high-power field (×40) using Image-Pro Express software.
Chi-square test and correlation coefficient were used for statistical analysis.
A statistically significant difference in the values of MVD and MCD between normal gingival tissue, leukoplakia with and without dysplasia and OSCC (
= 0.000) was observed. MVD and MCD showed a positive correlation between the study groups.
MVD and MCD increased significantly in cases of OSCC as compared to leukoplakia with and without dysplasia and normal gingival tissue. It was concluded that MCs may play a significant role in angiogenesis by releasing pro-angiogenic and angiogenic factors which may in turn favor the progression of premalignant lesion to a malignant one.</abstract><cop>India</cop><pub>Medknow Publications and Media Pvt. Ltd</pub><pmid>28932032</pmid><doi>10.4103/jomfp.JOMFP_111_15</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0973-029X |
| ispartof | Journal of oral and maxillofacial pathology : JOMFP, 2017-05, Vol.21 (2), p.229-238 |
| issn | 0973-029X 1998-393X |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5596673 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Medknow Open Access Journals; PubMed Central |
| subjects | Angiogenesis Antigens CD34 antigen Cell density Dysplasia Endothelium Gingiva Hypotheses Immunohistochemistry Leukokeratosis Mast cells Metastasis Mouth cancer Neovascularization Oral cancer Oral squamous cell carcinoma Original Pathology Squamous cell carcinoma Stains & staining Statistical analysis Statistics Toluidine Tumors |
| title | Mast cells and angiogenesis in malignant and premalignant oral lesions: An immunohistochemical study |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T20%3A41%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Mast%20cells%20and%20angiogenesis%20in%20malignant%20and%20premalignant%20oral%20lesions:%20An%20immunohistochemical%20study&rft.jtitle=Journal%20of%20oral%20and%20maxillofacial%20pathology%20:%20JOMFP&rft.au=Laishram,%20Daisy&rft.date=2017-05-01&rft.volume=21&rft.issue=2&rft.spage=229&rft.epage=238&rft.pages=229-238&rft.issn=0973-029X&rft.eissn=1998-393X&rft_id=info:doi/10.4103/jomfp.JOMFP_111_15&rft_dat=%3Cgale_pubme%3EA501344910%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1942269537&rft_id=info:pmid/28932032&rft_galeid=A501344910&rfr_iscdi=true |