Identification of Purple Acid Phosphatases in Chickpea and Potential Roles of CaPAP7 in Seed Phytate Accumulation
Purple acid phosphatases (PAPs) play important roles in phosphate (Pi) acquisition and utilization. These PAPs hydrolyze organic Phosphorus (P) containing compounds in rhizosphere as well as inside the plant cell. However, roles of PAPs in one of the most widely cultivated legumes, chickpea ( Cicer...
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| Veröffentlicht in: | Scientific reports 2017-09, Vol.7 (1), p.11012-12, Article 11012 |
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| description | Purple acid phosphatases (PAPs) play important roles in phosphate (Pi) acquisition and utilization. These PAPs hydrolyze organic Phosphorus (P) containing compounds in rhizosphere as well as inside the plant cell. However, roles of PAPs in one of the most widely cultivated legumes, chickpea (
Cicer arietnum L
.), have not been unraveled so far. In the present study, we identified 25 putative PAPs in chickpea (CaPAPs) which possess functional PAP motifs and domains. Differential regulation of
CaPAP
s under different nutrient deficiencies revealed their roles under multiple nutrient stresses including Pi deficiency. Interestingly, most of the
CaPAP
s were prominently expressed in flowers and young pods indicating their roles in flower and seed development. Association mapping of SNPs underlying
CaPAP
s with seed traits revealed significant association of low Pi inducible
CaPAP
7 with seed weight and phytate content. Biochemical characterization of recombinant CaPAP7 established it to be a functional acid phosphatase with highest activity on most abundant organic-P substrate, phytate. Exogenous application of recombinant CaPAP7 enhanced biomass and Pi content of Arabidopsis seedlings supplemented with phytate as sole P source. Taken together, our results uncover the PAPs in chickpea and potential roles of CaPAP7 in seed phytate accumulation. |
| doi_str_mv | 10.1038/s41598-017-11490-9 |
| format | Article |
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Cicer arietnum L
.), have not been unraveled so far. In the present study, we identified 25 putative PAPs in chickpea (CaPAPs) which possess functional PAP motifs and domains. Differential regulation of
CaPAP
s under different nutrient deficiencies revealed their roles under multiple nutrient stresses including Pi deficiency. Interestingly, most of the
CaPAP
s were prominently expressed in flowers and young pods indicating their roles in flower and seed development. Association mapping of SNPs underlying
CaPAP
s with seed traits revealed significant association of low Pi inducible
CaPAP
7 with seed weight and phytate content. Biochemical characterization of recombinant CaPAP7 established it to be a functional acid phosphatase with highest activity on most abundant organic-P substrate, phytate. Exogenous application of recombinant CaPAP7 enhanced biomass and Pi content of Arabidopsis seedlings supplemented with phytate as sole P source. Taken together, our results uncover the PAPs in chickpea and potential roles of CaPAP7 in seed phytate accumulation.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-017-11490-9</identifier><identifier>PMID: 28887557</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>38 ; 38/77 ; 38/90 ; 42 ; 45 ; 45/43 ; 45/77 ; 631/449/1659 ; 631/449/2661/2665 ; 82/80 ; Acid Phosphatase - metabolism ; Arabidopsis - genetics ; Arabidopsis - growth & development ; Arabidopsis - metabolism ; Cicer - enzymology ; Cicer - metabolism ; Cicer arietinum ; Cultivation ; Flowers ; Gene Expression ; Glycoproteins - metabolism ; Grain cultivation ; Humanities and Social Sciences ; Legumes ; multidisciplinary ; Nutrient deficiency ; Organic phosphorus ; Phosphatase ; Phosphorus ; Phytic Acid - metabolism ; Purple acid phosphatase ; Recombinant Proteins - metabolism ; Rhizosphere ; Science ; Science (multidisciplinary) ; Seedlings ; Seeds - metabolism</subject><ispartof>Scientific reports, 2017-09, Vol.7 (1), p.11012-12, Article 11012</ispartof><rights>The Author(s) 2017</rights><rights>2017. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-24447b92752f4b07b2de308cde82173690db8712f530211595f34d7cacac15bc3</citedby><cites>FETCH-LOGICAL-c474t-24447b92752f4b07b2de308cde82173690db8712f530211595f34d7cacac15bc3</cites><orcidid>0000-0002-8775-8249</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5591292/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5591292/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,27929,27930,41125,42194,51581,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28887557$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bhadouria, Jyoti</creatorcontrib><creatorcontrib>Singh, Ajit Pal</creatorcontrib><creatorcontrib>Mehra, Poonam</creatorcontrib><creatorcontrib>Verma, Lokesh</creatorcontrib><creatorcontrib>Srivastawa, Rishi</creatorcontrib><creatorcontrib>Parida, Swarup K.</creatorcontrib><creatorcontrib>Giri, Jitender</creatorcontrib><title>Identification of Purple Acid Phosphatases in Chickpea and Potential Roles of CaPAP7 in Seed Phytate Accumulation</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Purple acid phosphatases (PAPs) play important roles in phosphate (Pi) acquisition and utilization. These PAPs hydrolyze organic Phosphorus (P) containing compounds in rhizosphere as well as inside the plant cell. However, roles of PAPs in one of the most widely cultivated legumes, chickpea (
Cicer arietnum L
.), have not been unraveled so far. In the present study, we identified 25 putative PAPs in chickpea (CaPAPs) which possess functional PAP motifs and domains. Differential regulation of
CaPAP
s under different nutrient deficiencies revealed their roles under multiple nutrient stresses including Pi deficiency. Interestingly, most of the
CaPAP
s were prominently expressed in flowers and young pods indicating their roles in flower and seed development. Association mapping of SNPs underlying
CaPAP
s with seed traits revealed significant association of low Pi inducible
CaPAP
7 with seed weight and phytate content. Biochemical characterization of recombinant CaPAP7 established it to be a functional acid phosphatase with highest activity on most abundant organic-P substrate, phytate. Exogenous application of recombinant CaPAP7 enhanced biomass and Pi content of Arabidopsis seedlings supplemented with phytate as sole P source. Taken together, our results uncover the PAPs in chickpea and potential roles of CaPAP7 in seed phytate accumulation.</description><subject>38</subject><subject>38/77</subject><subject>38/90</subject><subject>42</subject><subject>45</subject><subject>45/43</subject><subject>45/77</subject><subject>631/449/1659</subject><subject>631/449/2661/2665</subject><subject>82/80</subject><subject>Acid Phosphatase - metabolism</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - growth & development</subject><subject>Arabidopsis - metabolism</subject><subject>Cicer - enzymology</subject><subject>Cicer - metabolism</subject><subject>Cicer arietinum</subject><subject>Cultivation</subject><subject>Flowers</subject><subject>Gene Expression</subject><subject>Glycoproteins - metabolism</subject><subject>Grain cultivation</subject><subject>Humanities and Social Sciences</subject><subject>Legumes</subject><subject>multidisciplinary</subject><subject>Nutrient deficiency</subject><subject>Organic phosphorus</subject><subject>Phosphatase</subject><subject>Phosphorus</subject><subject>Phytic Acid - 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metabolism</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - growth & development</topic><topic>Arabidopsis - metabolism</topic><topic>Cicer - enzymology</topic><topic>Cicer - metabolism</topic><topic>Cicer arietinum</topic><topic>Cultivation</topic><topic>Flowers</topic><topic>Gene Expression</topic><topic>Glycoproteins - metabolism</topic><topic>Grain cultivation</topic><topic>Humanities and Social Sciences</topic><topic>Legumes</topic><topic>multidisciplinary</topic><topic>Nutrient deficiency</topic><topic>Organic phosphorus</topic><topic>Phosphatase</topic><topic>Phosphorus</topic><topic>Phytic Acid - metabolism</topic><topic>Purple acid phosphatase</topic><topic>Recombinant Proteins - metabolism</topic><topic>Rhizosphere</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Seedlings</topic><topic>Seeds - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bhadouria, Jyoti</creatorcontrib><creatorcontrib>Singh, Ajit Pal</creatorcontrib><creatorcontrib>Mehra, Poonam</creatorcontrib><creatorcontrib>Verma, Lokesh</creatorcontrib><creatorcontrib>Srivastawa, Rishi</creatorcontrib><creatorcontrib>Parida, Swarup K.</creatorcontrib><creatorcontrib>Giri, Jitender</creatorcontrib><collection>Springer Nature OA/Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>Proquest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bhadouria, Jyoti</au><au>Singh, Ajit Pal</au><au>Mehra, Poonam</au><au>Verma, Lokesh</au><au>Srivastawa, Rishi</au><au>Parida, Swarup K.</au><au>Giri, Jitender</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of Purple Acid Phosphatases in Chickpea and Potential Roles of CaPAP7 in Seed Phytate Accumulation</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2017-09-08</date><risdate>2017</risdate><volume>7</volume><issue>1</issue><spage>11012</spage><epage>12</epage><pages>11012-12</pages><artnum>11012</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Purple acid phosphatases (PAPs) play important roles in phosphate (Pi) acquisition and utilization. These PAPs hydrolyze organic Phosphorus (P) containing compounds in rhizosphere as well as inside the plant cell. However, roles of PAPs in one of the most widely cultivated legumes, chickpea (
Cicer arietnum L
.), have not been unraveled so far. In the present study, we identified 25 putative PAPs in chickpea (CaPAPs) which possess functional PAP motifs and domains. Differential regulation of
CaPAP
s under different nutrient deficiencies revealed their roles under multiple nutrient stresses including Pi deficiency. Interestingly, most of the
CaPAP
s were prominently expressed in flowers and young pods indicating their roles in flower and seed development. Association mapping of SNPs underlying
CaPAP
s with seed traits revealed significant association of low Pi inducible
CaPAP
7 with seed weight and phytate content. Biochemical characterization of recombinant CaPAP7 established it to be a functional acid phosphatase with highest activity on most abundant organic-P substrate, phytate. Exogenous application of recombinant CaPAP7 enhanced biomass and Pi content of Arabidopsis seedlings supplemented with phytate as sole P source. Taken together, our results uncover the PAPs in chickpea and potential roles of CaPAP7 in seed phytate accumulation.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>28887557</pmid><doi>10.1038/s41598-017-11490-9</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-8775-8249</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 38 38/77 38/90 42 45 45/43 45/77 631/449/1659 631/449/2661/2665 82/80 Acid Phosphatase - metabolism Arabidopsis - genetics Arabidopsis - growth & development Arabidopsis - metabolism Cicer - enzymology Cicer - metabolism Cicer arietinum Cultivation Flowers Gene Expression Glycoproteins - metabolism Grain cultivation Humanities and Social Sciences Legumes multidisciplinary Nutrient deficiency Organic phosphorus Phosphatase Phosphorus Phytic Acid - metabolism Purple acid phosphatase Recombinant Proteins - metabolism Rhizosphere Science Science (multidisciplinary) Seedlings Seeds - metabolism |
| title | Identification of Purple Acid Phosphatases in Chickpea and Potential Roles of CaPAP7 in Seed Phytate Accumulation |
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