Label-free and real-time monitoring of single cell attachment on template-stripped plasmonic nano-holes
Leveraging microfluidics and nano-plasmonics, we present in this paper a new method employing a micro-nano-device that is capable of monitoring the dynamic cell-substrate attachment process at single cell level in real time without labeling. The micro-nano-device essentially has a gold thin film as...
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description | Leveraging microfluidics and nano-plasmonics, we present in this paper a new method employing a micro-nano-device that is capable of monitoring the dynamic cell-substrate attachment process at single cell level in real time without labeling. The micro-nano-device essentially has a gold thin film as the substrate perforated with periodic, near-cm
2
-area, template-stripped nano-holes, which generate plasmonic extraordinary optical transmission (EOT) with a high sensitivity to refractive index changes at the metal-dielectric interface. Using this device, we successfully demonstrated label-free and real-time monitoring of the dynamic cell attachment process for single mouse embryonic stem cell (C3H10) and human tumor cell (HeLa) by collecting EOT spectrum data during 3-hour on-chip culture. We further collected the EOT spectral shift data at the start and end points of measurement during 3-hour on-chip culture for 50 C3H10 and 50 HeLa cells, respectively. The experiment results show that the single cell attachment process of both HeLa and C3H10 cells follow the logistic retarded growth model, but with different kinetic parameters. Variations in spectral shift during the same culture period across single cells present new evidence for cell heterogeneity. The micro-nano-device provides a new, label-free, real-time, and sensitive, platform to investigate the cell adhesion kinetics at single cell level. |
doi_str_mv | 10.1038/s41598-017-11383-x |
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2
-area, template-stripped nano-holes, which generate plasmonic extraordinary optical transmission (EOT) with a high sensitivity to refractive index changes at the metal-dielectric interface. Using this device, we successfully demonstrated label-free and real-time monitoring of the dynamic cell attachment process for single mouse embryonic stem cell (C3H10) and human tumor cell (HeLa) by collecting EOT spectrum data during 3-hour on-chip culture. We further collected the EOT spectral shift data at the start and end points of measurement during 3-hour on-chip culture for 50 C3H10 and 50 HeLa cells, respectively. The experiment results show that the single cell attachment process of both HeLa and C3H10 cells follow the logistic retarded growth model, but with different kinetic parameters. Variations in spectral shift during the same culture period across single cells present new evidence for cell heterogeneity. The micro-nano-device provides a new, label-free, real-time, and sensitive, platform to investigate the cell adhesion kinetics at single cell level.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-017-11383-x</identifier><identifier>PMID: 28887548</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/56 ; 142/126 ; 631/1647/1888/2005 ; 631/61/350/1058 ; 82/103 ; 96/10 ; 96/63 ; Animals ; Cell Adhesion ; Cell adhesion & migration ; Cell culture ; Epithelial Cells - physiology ; HeLa Cells ; Heterogeneity ; Humanities and Social Sciences ; Humans ; Mice ; Microfluidics ; Microfluidics - instrumentation ; Microfluidics - methods ; Mouse Embryonic Stem Cells - physiology ; multidisciplinary ; Science ; Science (multidisciplinary) ; Single-Cell Analysis - instrumentation ; Single-Cell Analysis - methods ; Stem cells ; Thin films</subject><ispartof>Scientific reports, 2017-09, Vol.7 (1), p.11020-11, Article 11020</ispartof><rights>The Author(s) 2017</rights><rights>2017. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-8972aadbbe3902eb5f2fbc841b61fe7cc8169b2cf72d5c19c2f37f23280592cd3</citedby><cites>FETCH-LOGICAL-c540t-8972aadbbe3902eb5f2fbc841b61fe7cc8169b2cf72d5c19c2f37f23280592cd3</cites><orcidid>0000-0001-8198-2234</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5591264/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5591264/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28887548$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tu, Long</creatorcontrib><creatorcontrib>Li, Xuzhou</creatorcontrib><creatorcontrib>Bian, Shengtai</creatorcontrib><creatorcontrib>Yu, Yingting</creatorcontrib><creatorcontrib>Li, Junxiang</creatorcontrib><creatorcontrib>Huang, Liang</creatorcontrib><creatorcontrib>Liu, Peng</creatorcontrib><creatorcontrib>Wu, Qiong</creatorcontrib><creatorcontrib>Wang, Wenhui</creatorcontrib><title>Label-free and real-time monitoring of single cell attachment on template-stripped plasmonic nano-holes</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Leveraging microfluidics and nano-plasmonics, we present in this paper a new method employing a micro-nano-device that is capable of monitoring the dynamic cell-substrate attachment process at single cell level in real time without labeling. The micro-nano-device essentially has a gold thin film as the substrate perforated with periodic, near-cm
2
-area, template-stripped nano-holes, which generate plasmonic extraordinary optical transmission (EOT) with a high sensitivity to refractive index changes at the metal-dielectric interface. Using this device, we successfully demonstrated label-free and real-time monitoring of the dynamic cell attachment process for single mouse embryonic stem cell (C3H10) and human tumor cell (HeLa) by collecting EOT spectrum data during 3-hour on-chip culture. We further collected the EOT spectral shift data at the start and end points of measurement during 3-hour on-chip culture for 50 C3H10 and 50 HeLa cells, respectively. The experiment results show that the single cell attachment process of both HeLa and C3H10 cells follow the logistic retarded growth model, but with different kinetic parameters. Variations in spectral shift during the same culture period across single cells present new evidence for cell heterogeneity. The micro-nano-device provides a new, label-free, real-time, and sensitive, platform to investigate the cell adhesion kinetics at single cell level.</description><subject>13/56</subject><subject>142/126</subject><subject>631/1647/1888/2005</subject><subject>631/61/350/1058</subject><subject>82/103</subject><subject>96/10</subject><subject>96/63</subject><subject>Animals</subject><subject>Cell Adhesion</subject><subject>Cell adhesion & migration</subject><subject>Cell culture</subject><subject>Epithelial Cells - physiology</subject><subject>HeLa Cells</subject><subject>Heterogeneity</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Mice</subject><subject>Microfluidics</subject><subject>Microfluidics - instrumentation</subject><subject>Microfluidics - methods</subject><subject>Mouse Embryonic Stem Cells - physiology</subject><subject>multidisciplinary</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Single-Cell Analysis - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tu, Long</au><au>Li, Xuzhou</au><au>Bian, Shengtai</au><au>Yu, Yingting</au><au>Li, Junxiang</au><au>Huang, Liang</au><au>Liu, Peng</au><au>Wu, Qiong</au><au>Wang, Wenhui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Label-free and real-time monitoring of single cell attachment on template-stripped plasmonic nano-holes</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2017-09-08</date><risdate>2017</risdate><volume>7</volume><issue>1</issue><spage>11020</spage><epage>11</epage><pages>11020-11</pages><artnum>11020</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Leveraging microfluidics and nano-plasmonics, we present in this paper a new method employing a micro-nano-device that is capable of monitoring the dynamic cell-substrate attachment process at single cell level in real time without labeling. The micro-nano-device essentially has a gold thin film as the substrate perforated with periodic, near-cm
2
-area, template-stripped nano-holes, which generate plasmonic extraordinary optical transmission (EOT) with a high sensitivity to refractive index changes at the metal-dielectric interface. Using this device, we successfully demonstrated label-free and real-time monitoring of the dynamic cell attachment process for single mouse embryonic stem cell (C3H10) and human tumor cell (HeLa) by collecting EOT spectrum data during 3-hour on-chip culture. We further collected the EOT spectral shift data at the start and end points of measurement during 3-hour on-chip culture for 50 C3H10 and 50 HeLa cells, respectively. The experiment results show that the single cell attachment process of both HeLa and C3H10 cells follow the logistic retarded growth model, but with different kinetic parameters. Variations in spectral shift during the same culture period across single cells present new evidence for cell heterogeneity. The micro-nano-device provides a new, label-free, real-time, and sensitive, platform to investigate the cell adhesion kinetics at single cell level.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>28887548</pmid><doi>10.1038/s41598-017-11383-x</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-8198-2234</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | 13/56 142/126 631/1647/1888/2005 631/61/350/1058 82/103 96/10 96/63 Animals Cell Adhesion Cell adhesion & migration Cell culture Epithelial Cells - physiology HeLa Cells Heterogeneity Humanities and Social Sciences Humans Mice Microfluidics Microfluidics - instrumentation Microfluidics - methods Mouse Embryonic Stem Cells - physiology multidisciplinary Science Science (multidisciplinary) Single-Cell Analysis - instrumentation Single-Cell Analysis - methods Stem cells Thin films |
title | Label-free and real-time monitoring of single cell attachment on template-stripped plasmonic nano-holes |
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