Label-free and real-time monitoring of single cell attachment on template-stripped plasmonic nano-holes

Leveraging microfluidics and nano-plasmonics, we present in this paper a new method employing a micro-nano-device that is capable of monitoring the dynamic cell-substrate attachment process at single cell level in real time without labeling. The micro-nano-device essentially has a gold thin film as...

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Veröffentlicht in:Scientific reports 2017-09, Vol.7 (1), p.11020-11, Article 11020
Hauptverfasser: Tu, Long, Li, Xuzhou, Bian, Shengtai, Yu, Yingting, Li, Junxiang, Huang, Liang, Liu, Peng, Wu, Qiong, Wang, Wenhui
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container_title Scientific reports
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creator Tu, Long
Li, Xuzhou
Bian, Shengtai
Yu, Yingting
Li, Junxiang
Huang, Liang
Liu, Peng
Wu, Qiong
Wang, Wenhui
description Leveraging microfluidics and nano-plasmonics, we present in this paper a new method employing a micro-nano-device that is capable of monitoring the dynamic cell-substrate attachment process at single cell level in real time without labeling. The micro-nano-device essentially has a gold thin film as the substrate perforated with periodic, near-cm 2 -area, template-stripped nano-holes, which generate plasmonic extraordinary optical transmission (EOT) with a high sensitivity to refractive index changes at the metal-dielectric interface. Using this device, we successfully demonstrated label-free and real-time monitoring of the dynamic cell attachment process for single mouse embryonic stem cell (C3H10) and human tumor cell (HeLa) by collecting EOT spectrum data during 3-hour on-chip culture. We further collected the EOT spectral shift data at the start and end points of measurement during 3-hour on-chip culture for 50 C3H10 and 50 HeLa cells, respectively. The experiment results show that the single cell attachment process of both HeLa and C3H10 cells follow the logistic retarded growth model, but with different kinetic parameters. Variations in spectral shift during the same culture period across single cells present new evidence for cell heterogeneity. The micro-nano-device provides a new, label-free, real-time, and sensitive, platform to investigate the cell adhesion kinetics at single cell level.
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The micro-nano-device essentially has a gold thin film as the substrate perforated with periodic, near-cm 2 -area, template-stripped nano-holes, which generate plasmonic extraordinary optical transmission (EOT) with a high sensitivity to refractive index changes at the metal-dielectric interface. Using this device, we successfully demonstrated label-free and real-time monitoring of the dynamic cell attachment process for single mouse embryonic stem cell (C3H10) and human tumor cell (HeLa) by collecting EOT spectrum data during 3-hour on-chip culture. We further collected the EOT spectral shift data at the start and end points of measurement during 3-hour on-chip culture for 50 C3H10 and 50 HeLa cells, respectively. The experiment results show that the single cell attachment process of both HeLa and C3H10 cells follow the logistic retarded growth model, but with different kinetic parameters. 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subjects 13/56
142/126
631/1647/1888/2005
631/61/350/1058
82/103
96/10
96/63
Animals
Cell Adhesion
Cell adhesion & migration
Cell culture
Epithelial Cells - physiology
HeLa Cells
Heterogeneity
Humanities and Social Sciences
Humans
Mice
Microfluidics
Microfluidics - instrumentation
Microfluidics - methods
Mouse Embryonic Stem Cells - physiology
multidisciplinary
Science
Science (multidisciplinary)
Single-Cell Analysis - instrumentation
Single-Cell Analysis - methods
Stem cells
Thin films
title Label-free and real-time monitoring of single cell attachment on template-stripped plasmonic nano-holes
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