Virulence of current German PEDV strains in suckling pigs and investigation of protective effects of maternally derived antibodies
Porcine epidemic diarrhea (PED) has caused tremendous losses to the United States pig industry since 2013. From 2014, outbreaks were also reported from Central Europe. To characterize the Central European PEDV strains regarding their virulence in suckling piglets, and to assess the protective effect...
Gespeichert in:
| Veröffentlicht in: | Scientific reports 2017-09, Vol.7 (1), p.10825-11, Article 10825 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 11 |
|---|---|
| container_issue | 1 |
| container_start_page | 10825 |
| container_title | Scientific reports |
| container_volume | 7 |
| creator | Leidenberger, S. Schröder, Ch Zani, L. Auste, A. Pinette, M. Ambagala, A. Nikolin, V. de Smit, H. Beer, M. Blome, S. |
| description | Porcine epidemic diarrhea (PED) has caused tremendous losses to the United States pig industry since 2013. From 2014, outbreaks were also reported from Central Europe. To characterize the Central European PEDV strains regarding their virulence in suckling piglets, and to assess the protective effect of maternally derived antibodies (MDA), four trial groups were randomly assigned, each consisting of two pregnant sows and their litter. To induce MDA in a subset of piglets, two sows received a cell culture-adapted PEDV strain, and another two sows were inoculated with field material from German PED outbreaks. Four sows stayed naïve. Subsequently, all piglets were inoculated with the corresponding PEDV strains at an age of 3 to 6 days, and virus shedding, clinical signs and occurrence of specific antibodies were assessed. Piglets without MDA showed a morbidity of 100% and low lethality, while almost all MDA-positive piglets stayed clinically healthy and showed considerably lower virus shedding. Taken together, the Central European PEDV strains showed rather low virulence under experimental conditions, and pre-inoculation of sows led to a solid protection of their offspring. The latter is the prerequisite for a sow vaccination concept that could help to prevent PED induced losses in the piglet sector. |
| doi_str_mv | 10.1038/s41598-017-11160-w |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5589859</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1957749609</sourcerecordid><originalsourceid>FETCH-LOGICAL-c540t-2a03fd1aaeaa0b37cc505e63c54429cffb269d42e0953011bf8b86b74c8980c73</originalsourceid><addsrcrecordid>eNp1kU9vFSEUxYmxsU3bL-DCkLhxM8qfYQY2Jqat1aRJXWi3hGHujNQZeALzmm795DK-2ryayAZyz7k_uByEXlLylhIu36WaCiUrQtuKUtqQ6u4ZOmKkFhXjjD3fOx-i05RuSVmCqZqqF-iQSSl5w-QR-nXj4jKBt4DDgO0SI_iMLyHOxuMvF-c3OOVonE_YeZwW-2NyfsQbNyZsfF-KW0jZjSa74FfCJoYMNrstYBiGckprdTYZojfTdI97iEXsS3d2XegdpBN0MJgpwenDfoy-fbz4evapurq-_Hz24aqyoia5YobwoafGgDGk4621gghoeFFrpuwwdKxRfc2AKMEJpd0gO9l0bW2lksS2_Bi933E3SzdDb8ug0Ux6E91s4r0Oxumninff9Ri2WohCEKoA3jwAYvi5lLn17JKFaTIewpI0VbwVVApWF-vrf6y3YVl_YHWJtq1VQ1Yg27lsDClFGB4fQ4leU9a7lHVJWf9JWd-Vplf7Yzy2_M20GPjOkIrkR4h7d_8f-xs7CrZO</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1957749609</pqid></control><display><type>article</type><title>Virulence of current German PEDV strains in suckling pigs and investigation of protective effects of maternally derived antibodies</title><source>MEDLINE</source><source>Nature Free</source><source>Free E-Journal (出版社公開部分のみ）</source><source>PubMed Central</source><source>Directory of Open Access Journals</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><source>Springer Nature OA Free Journals</source><creator>Leidenberger, S. ; Schröder, Ch ; Zani, L. ; Auste, A. ; Pinette, M. ; Ambagala, A. ; Nikolin, V. ; de Smit, H. ; Beer, M. ; Blome, S.</creator><creatorcontrib>Leidenberger, S. ; Schröder, Ch ; Zani, L. ; Auste, A. ; Pinette, M. ; Ambagala, A. ; Nikolin, V. ; de Smit, H. ; Beer, M. ; Blome, S.</creatorcontrib><description>Porcine epidemic diarrhea (PED) has caused tremendous losses to the United States pig industry since 2013. From 2014, outbreaks were also reported from Central Europe. To characterize the Central European PEDV strains regarding their virulence in suckling piglets, and to assess the protective effect of maternally derived antibodies (MDA), four trial groups were randomly assigned, each consisting of two pregnant sows and their litter. To induce MDA in a subset of piglets, two sows received a cell culture-adapted PEDV strain, and another two sows were inoculated with field material from German PED outbreaks. Four sows stayed naïve. Subsequently, all piglets were inoculated with the corresponding PEDV strains at an age of 3 to 6 days, and virus shedding, clinical signs and occurrence of specific antibodies were assessed. Piglets without MDA showed a morbidity of 100% and low lethality, while almost all MDA-positive piglets stayed clinically healthy and showed considerably lower virus shedding. Taken together, the Central European PEDV strains showed rather low virulence under experimental conditions, and pre-inoculation of sows led to a solid protection of their offspring. The latter is the prerequisite for a sow vaccination concept that could help to prevent PED induced losses in the piglet sector.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-017-11160-w</identifier><identifier>PMID: 28883628</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/250/255/2514 ; 631/326/596/2555 ; Animals ; Antibodies ; Antibodies, Viral - blood ; Cell culture ; Coronavirus Infections - immunology ; Coronavirus Infections - pathology ; Coronavirus Infections - veterinary ; Coronavirus Infections - virology ; Diarrhea ; Germany ; Hogs ; Humanities and Social Sciences ; Immunity, Maternally-Acquired ; Immunoglobulins ; Inoculation ; Lethality ; Morbidity ; multidisciplinary ; Offspring ; Outbreaks ; Porcine epidemic diarrhea virus - immunology ; Porcine epidemic diarrhea virus - isolation & purification ; Porcine epidemic diarrhea virus - pathogenicity ; Pregnancy ; Science ; Science (multidisciplinary) ; Strains (organisms) ; Suckling behavior ; Survival Analysis ; Swine ; Swine Diseases - immunology ; Swine Diseases - pathology ; Swine Diseases - virology ; Transmissible gastroenteritis ; Vaccination ; Virulence ; Virus Shedding</subject><ispartof>Scientific reports, 2017-09, Vol.7 (1), p.10825-11, Article 10825</ispartof><rights>The Author(s) 2017</rights><rights>2017. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-2a03fd1aaeaa0b37cc505e63c54429cffb269d42e0953011bf8b86b74c8980c73</citedby><cites>FETCH-LOGICAL-c540t-2a03fd1aaeaa0b37cc505e63c54429cffb269d42e0953011bf8b86b74c8980c73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5589859/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5589859/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28883628$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Leidenberger, S.</creatorcontrib><creatorcontrib>Schröder, Ch</creatorcontrib><creatorcontrib>Zani, L.</creatorcontrib><creatorcontrib>Auste, A.</creatorcontrib><creatorcontrib>Pinette, M.</creatorcontrib><creatorcontrib>Ambagala, A.</creatorcontrib><creatorcontrib>Nikolin, V.</creatorcontrib><creatorcontrib>de Smit, H.</creatorcontrib><creatorcontrib>Beer, M.</creatorcontrib><creatorcontrib>Blome, S.</creatorcontrib><title>Virulence of current German PEDV strains in suckling pigs and investigation of protective effects of maternally derived antibodies</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Porcine epidemic diarrhea (PED) has caused tremendous losses to the United States pig industry since 2013. From 2014, outbreaks were also reported from Central Europe. To characterize the Central European PEDV strains regarding their virulence in suckling piglets, and to assess the protective effect of maternally derived antibodies (MDA), four trial groups were randomly assigned, each consisting of two pregnant sows and their litter. To induce MDA in a subset of piglets, two sows received a cell culture-adapted PEDV strain, and another two sows were inoculated with field material from German PED outbreaks. Four sows stayed naïve. Subsequently, all piglets were inoculated with the corresponding PEDV strains at an age of 3 to 6 days, and virus shedding, clinical signs and occurrence of specific antibodies were assessed. Piglets without MDA showed a morbidity of 100% and low lethality, while almost all MDA-positive piglets stayed clinically healthy and showed considerably lower virus shedding. Taken together, the Central European PEDV strains showed rather low virulence under experimental conditions, and pre-inoculation of sows led to a solid protection of their offspring. The latter is the prerequisite for a sow vaccination concept that could help to prevent PED induced losses in the piglet sector.</description><subject>631/250/255/2514</subject><subject>631/326/596/2555</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Viral - blood</subject><subject>Cell culture</subject><subject>Coronavirus Infections - immunology</subject><subject>Coronavirus Infections - pathology</subject><subject>Coronavirus Infections - veterinary</subject><subject>Coronavirus Infections - virology</subject><subject>Diarrhea</subject><subject>Germany</subject><subject>Hogs</subject><subject>Humanities and Social Sciences</subject><subject>Immunity, Maternally-Acquired</subject><subject>Immunoglobulins</subject><subject>Inoculation</subject><subject>Lethality</subject><subject>Morbidity</subject><subject>multidisciplinary</subject><subject>Offspring</subject><subject>Outbreaks</subject><subject>Porcine epidemic diarrhea virus - immunology</subject><subject>Porcine epidemic diarrhea virus - isolation & purification</subject><subject>Porcine epidemic diarrhea virus - pathogenicity</subject><subject>Pregnancy</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Strains (organisms)</subject><subject>Suckling behavior</subject><subject>Survival Analysis</subject><subject>Swine</subject><subject>Swine Diseases - immunology</subject><subject>Swine Diseases - pathology</subject><subject>Swine Diseases - virology</subject><subject>Transmissible gastroenteritis</subject><subject>Vaccination</subject><subject>Virulence</subject><subject>Virus Shedding</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kU9vFSEUxYmxsU3bL-DCkLhxM8qfYQY2Jqat1aRJXWi3hGHujNQZeALzmm795DK-2ryayAZyz7k_uByEXlLylhIu36WaCiUrQtuKUtqQ6u4ZOmKkFhXjjD3fOx-i05RuSVmCqZqqF-iQSSl5w-QR-nXj4jKBt4DDgO0SI_iMLyHOxuMvF-c3OOVonE_YeZwW-2NyfsQbNyZsfF-KW0jZjSa74FfCJoYMNrstYBiGckprdTYZojfTdI97iEXsS3d2XegdpBN0MJgpwenDfoy-fbz4evapurq-_Hz24aqyoia5YobwoafGgDGk4621gghoeFFrpuwwdKxRfc2AKMEJpd0gO9l0bW2lksS2_Bi933E3SzdDb8ug0Ux6E91s4r0Oxumninff9Ri2WohCEKoA3jwAYvi5lLn17JKFaTIewpI0VbwVVApWF-vrf6y3YVl_YHWJtq1VQ1Yg27lsDClFGB4fQ4leU9a7lHVJWf9JWd-Vplf7Yzy2_M20GPjOkIrkR4h7d_8f-xs7CrZO</recordid><startdate>20170907</startdate><enddate>20170907</enddate><creator>Leidenberger, S.</creator><creator>Schröder, Ch</creator><creator>Zani, L.</creator><creator>Auste, A.</creator><creator>Pinette, M.</creator><creator>Ambagala, A.</creator><creator>Nikolin, V.</creator><creator>de Smit, H.</creator><creator>Beer, M.</creator><creator>Blome, S.</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170907</creationdate><title>Virulence of current German PEDV strains in suckling pigs and investigation of protective effects of maternally derived antibodies</title><author>Leidenberger, S. ; Schröder, Ch ; Zani, L. ; Auste, A. ; Pinette, M. ; Ambagala, A. ; Nikolin, V. ; de Smit, H. ; Beer, M. ; Blome, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540t-2a03fd1aaeaa0b37cc505e63c54429cffb269d42e0953011bf8b86b74c8980c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>631/250/255/2514</topic><topic>631/326/596/2555</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Viral - blood</topic><topic>Cell culture</topic><topic>Coronavirus Infections - immunology</topic><topic>Coronavirus Infections - pathology</topic><topic>Coronavirus Infections - veterinary</topic><topic>Coronavirus Infections - virology</topic><topic>Diarrhea</topic><topic>Germany</topic><topic>Hogs</topic><topic>Humanities and Social Sciences</topic><topic>Immunity, Maternally-Acquired</topic><topic>Immunoglobulins</topic><topic>Inoculation</topic><topic>Lethality</topic><topic>Morbidity</topic><topic>multidisciplinary</topic><topic>Offspring</topic><topic>Outbreaks</topic><topic>Porcine epidemic diarrhea virus - immunology</topic><topic>Porcine epidemic diarrhea virus - isolation & purification</topic><topic>Porcine epidemic diarrhea virus - pathogenicity</topic><topic>Pregnancy</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Strains (organisms)</topic><topic>Suckling behavior</topic><topic>Survival Analysis</topic><topic>Swine</topic><topic>Swine Diseases - immunology</topic><topic>Swine Diseases - pathology</topic><topic>Swine Diseases - virology</topic><topic>Transmissible gastroenteritis</topic><topic>Vaccination</topic><topic>Virulence</topic><topic>Virus Shedding</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Leidenberger, S.</creatorcontrib><creatorcontrib>Schröder, Ch</creatorcontrib><creatorcontrib>Zani, L.</creatorcontrib><creatorcontrib>Auste, A.</creatorcontrib><creatorcontrib>Pinette, M.</creatorcontrib><creatorcontrib>Ambagala, A.</creatorcontrib><creatorcontrib>Nikolin, V.</creatorcontrib><creatorcontrib>de Smit, H.</creatorcontrib><creatorcontrib>Beer, M.</creatorcontrib><creatorcontrib>Blome, S.</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Complete (ProQuest Database)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Science Journals (ProQuest Database)</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leidenberger, S.</au><au>Schröder, Ch</au><au>Zani, L.</au><au>Auste, A.</au><au>Pinette, M.</au><au>Ambagala, A.</au><au>Nikolin, V.</au><au>de Smit, H.</au><au>Beer, M.</au><au>Blome, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Virulence of current German PEDV strains in suckling pigs and investigation of protective effects of maternally derived antibodies</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2017-09-07</date><risdate>2017</risdate><volume>7</volume><issue>1</issue><spage>10825</spage><epage>11</epage><pages>10825-11</pages><artnum>10825</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Porcine epidemic diarrhea (PED) has caused tremendous losses to the United States pig industry since 2013. From 2014, outbreaks were also reported from Central Europe. To characterize the Central European PEDV strains regarding their virulence in suckling piglets, and to assess the protective effect of maternally derived antibodies (MDA), four trial groups were randomly assigned, each consisting of two pregnant sows and their litter. To induce MDA in a subset of piglets, two sows received a cell culture-adapted PEDV strain, and another two sows were inoculated with field material from German PED outbreaks. Four sows stayed naïve. Subsequently, all piglets were inoculated with the corresponding PEDV strains at an age of 3 to 6 days, and virus shedding, clinical signs and occurrence of specific antibodies were assessed. Piglets without MDA showed a morbidity of 100% and low lethality, while almost all MDA-positive piglets stayed clinically healthy and showed considerably lower virus shedding. Taken together, the Central European PEDV strains showed rather low virulence under experimental conditions, and pre-inoculation of sows led to a solid protection of their offspring. The latter is the prerequisite for a sow vaccination concept that could help to prevent PED induced losses in the piglet sector.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>28883628</pmid><doi>10.1038/s41598-017-11160-w</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2045-2322 |
| ispartof | Scientific reports, 2017-09, Vol.7 (1), p.10825-11, Article 10825 |
| issn | 2045-2322 2045-2322 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5589859 |
| source | MEDLINE; Nature Free; Free E-Journal (出版社公開部分のみ）; PubMed Central; Directory of Open Access Journals; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; Springer Nature OA Free Journals |
| subjects | 631/250/255/2514 631/326/596/2555 Animals Antibodies Antibodies, Viral - blood Cell culture Coronavirus Infections - immunology Coronavirus Infections - pathology Coronavirus Infections - veterinary Coronavirus Infections - virology Diarrhea Germany Hogs Humanities and Social Sciences Immunity, Maternally-Acquired Immunoglobulins Inoculation Lethality Morbidity multidisciplinary Offspring Outbreaks Porcine epidemic diarrhea virus - immunology Porcine epidemic diarrhea virus - isolation & purification Porcine epidemic diarrhea virus - pathogenicity Pregnancy Science Science (multidisciplinary) Strains (organisms) Suckling behavior Survival Analysis Swine Swine Diseases - immunology Swine Diseases - pathology Swine Diseases - virology Transmissible gastroenteritis Vaccination Virulence Virus Shedding |
| title | Virulence of current German PEDV strains in suckling pigs and investigation of protective effects of maternally derived antibodies |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T18%3A11%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Virulence%20of%20current%20German%20PEDV%20strains%20in%20suckling%20pigs%20and%20investigation%20of%20protective%20effects%20of%20maternally%20derived%20antibodies&rft.jtitle=Scientific%20reports&rft.au=Leidenberger,%20S.&rft.date=2017-09-07&rft.volume=7&rft.issue=1&rft.spage=10825&rft.epage=11&rft.pages=10825-11&rft.artnum=10825&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/s41598-017-11160-w&rft_dat=%3Cproquest_pubme%3E1957749609%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1957749609&rft_id=info:pmid/28883628&rfr_iscdi=true |