Feedback Microtubule Control and Microtubule-Actin Cross-talk in Arabidopsis Revealed by Integrative Proteomic and Cell Biology Analysis of KATANIN 1 Mutants
Microtubule organization and dynamics are critical for key developmental processes such as cell division, elongation, and morphogenesis. Microtubule severing is an essential regulator of microtubules and is exclusively executed by KATANIN 1 in Arabidopsis. In this study, we comparatively studied the...
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| Veröffentlicht in: | Molecular & cellular proteomics 2017-09, Vol.16 (9), p.1591-1609 |
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| creator | Takáč, Tomáš Šamajová, Olga Pechan, Tibor Luptovčiak, Ivan Šamaj, Jozef |
| description | Microtubule organization and dynamics are critical for key developmental processes such as cell division, elongation, and morphogenesis. Microtubule severing is an essential regulator of microtubules and is exclusively executed by KATANIN 1 in Arabidopsis. In this study, we comparatively studied the proteome-wide effects in two KATANIN 1 mutants. Thus, shotgun proteomic analysis of roots and aerial parts of single nucleotide mutant fra2 and T-DNA insertion mutant ktn1-2 was carried out. We have detected 42 proteins differentially abundant in both fra2 and ktn1-2. KATANIN 1 dysfunction altered the abundance of proteins involved in development, metabolism, and stress responses. The differential regulation of tubulins and microtubule-destabilizing protein MDP25 implied a feedback microtubule control in KATANIN 1 mutants. Furthermore, deregulation of profilin 1, actin-depolymerizing factor 3, and actin 7 was observed. These findings were confirmed by immunoblotting analysis of actin and by microscopic observation of actin filaments using fluorescently labeled phalloidin. Results obtained by quantitative RT-PCR analysis revealed that changed protein abundances were not a consequence of altered expression levels of corresponding genes in the mutants. In conclusion, we show that abundances of several cytoskeletal proteins as well as organization of microtubules and the actin cytoskeleton are amended in accordance with defective microtubule severing. |
| doi_str_mv | 10.1074/mcp.M117.068015 |
| format | Article |
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Microtubule severing is an essential regulator of microtubules and is exclusively executed by KATANIN 1 in Arabidopsis. In this study, we comparatively studied the proteome-wide effects in two KATANIN 1 mutants. Thus, shotgun proteomic analysis of roots and aerial parts of single nucleotide mutant fra2 and T-DNA insertion mutant ktn1-2 was carried out. We have detected 42 proteins differentially abundant in both fra2 and ktn1-2. KATANIN 1 dysfunction altered the abundance of proteins involved in development, metabolism, and stress responses. The differential regulation of tubulins and microtubule-destabilizing protein MDP25 implied a feedback microtubule control in KATANIN 1 mutants. Furthermore, deregulation of profilin 1, actin-depolymerizing factor 3, and actin 7 was observed. These findings were confirmed by immunoblotting analysis of actin and by microscopic observation of actin filaments using fluorescently labeled phalloidin. Results obtained by quantitative RT-PCR analysis revealed that changed protein abundances were not a consequence of altered expression levels of corresponding genes in the mutants. In conclusion, we show that abundances of several cytoskeletal proteins as well as organization of microtubules and the actin cytoskeleton are amended in accordance with defective microtubule severing.</description><identifier>ISSN: 1535-9476</identifier><identifier>EISSN: 1535-9484</identifier><identifier>DOI: 10.1074/mcp.M117.068015</identifier><identifier>PMID: 28706004</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Actin ; Actin-depolymerizing protein ; Actins - metabolism ; Arabidopsis ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Cell Biology ; Cell division ; Control systems ; Cytoskeleton ; Deoxyribonucleic acid ; Depolymerization ; Deregulation ; DNA ; Elongation ; Feedback ; Feedback, Physiological ; Filaments ; Gene expression ; Gene Ontology ; Genes, Plant ; Immunoblotting ; Katanin - genetics ; Metabolism ; Microtubules ; Microtubules - metabolism ; Molecular Sequence Annotation ; Morphogenesis ; Mutants ; Mutation - genetics ; Phalloidin ; Polymerase chain reaction ; Profilin ; Protein Interaction Maps ; Proteins ; Proteome - metabolism ; Proteomics - methods ; T-DNA</subject><ispartof>Molecular & cellular proteomics, 2017-09, Vol.16 (9), p.1591-1609</ispartof><rights>2017 © 2017 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.</rights><rights>2017 by The American Society for Biochemistry and Molecular Biology, Inc.</rights><rights>Copyright American Society for Biochemistry and Molecular Biology Sep 2017</rights><rights>2017 by The American Society for Biochemistry and Molecular Biology, Inc. 2017 The American Society for Biochemistry and Molecular Biology, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-198ddf04b03620a7a43c9e87efe7745c99862acd8e1e1252efa443c05826ee2a3</citedby><cites>FETCH-LOGICAL-c471t-198ddf04b03620a7a43c9e87efe7745c99862acd8e1e1252efa443c05826ee2a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587860/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587860/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,725,778,782,883,27911,27912,53778,53780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28706004$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takáč, Tomáš</creatorcontrib><creatorcontrib>Šamajová, Olga</creatorcontrib><creatorcontrib>Pechan, Tibor</creatorcontrib><creatorcontrib>Luptovčiak, Ivan</creatorcontrib><creatorcontrib>Šamaj, Jozef</creatorcontrib><title>Feedback Microtubule Control and Microtubule-Actin Cross-talk in Arabidopsis Revealed by Integrative Proteomic and Cell Biology Analysis of KATANIN 1 Mutants</title><title>Molecular & cellular proteomics</title><addtitle>Mol Cell Proteomics</addtitle><description>Microtubule organization and dynamics are critical for key developmental processes such as cell division, elongation, and morphogenesis. Microtubule severing is an essential regulator of microtubules and is exclusively executed by KATANIN 1 in Arabidopsis. In this study, we comparatively studied the proteome-wide effects in two KATANIN 1 mutants. Thus, shotgun proteomic analysis of roots and aerial parts of single nucleotide mutant fra2 and T-DNA insertion mutant ktn1-2 was carried out. We have detected 42 proteins differentially abundant in both fra2 and ktn1-2. KATANIN 1 dysfunction altered the abundance of proteins involved in development, metabolism, and stress responses. The differential regulation of tubulins and microtubule-destabilizing protein MDP25 implied a feedback microtubule control in KATANIN 1 mutants. Furthermore, deregulation of profilin 1, actin-depolymerizing factor 3, and actin 7 was observed. These findings were confirmed by immunoblotting analysis of actin and by microscopic observation of actin filaments using fluorescently labeled phalloidin. Results obtained by quantitative RT-PCR analysis revealed that changed protein abundances were not a consequence of altered expression levels of corresponding genes in the mutants. In conclusion, we show that abundances of several cytoskeletal proteins as well as organization of microtubules and the actin cytoskeleton are amended in accordance with defective microtubule severing.</description><subject>Actin</subject><subject>Actin-depolymerizing protein</subject><subject>Actins - metabolism</subject><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Cell Biology</subject><subject>Cell division</subject><subject>Control systems</subject><subject>Cytoskeleton</subject><subject>Deoxyribonucleic acid</subject><subject>Depolymerization</subject><subject>Deregulation</subject><subject>DNA</subject><subject>Elongation</subject><subject>Feedback</subject><subject>Feedback, Physiological</subject><subject>Filaments</subject><subject>Gene expression</subject><subject>Gene Ontology</subject><subject>Genes, Plant</subject><subject>Immunoblotting</subject><subject>Katanin - genetics</subject><subject>Metabolism</subject><subject>Microtubules</subject><subject>Microtubules - metabolism</subject><subject>Molecular Sequence Annotation</subject><subject>Morphogenesis</subject><subject>Mutants</subject><subject>Mutation - genetics</subject><subject>Phalloidin</subject><subject>Polymerase chain reaction</subject><subject>Profilin</subject><subject>Protein Interaction Maps</subject><subject>Proteins</subject><subject>Proteome - metabolism</subject><subject>Proteomics - methods</subject><subject>T-DNA</subject><issn>1535-9476</issn><issn>1535-9484</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1u1DAUhSMEoqWwZocssWGTqZ04sbNBChEtIzoFobK2HPtmcOuJB9sZaR6Gd63DlFFBYuW_7x75nJNlrwleEMzo-UZtFytC2ALXHJPqSXZKqrLKG8rp0-Oe1SfZixBuMS4wYdXz7KTgDNcY09Ps1wWA7qW6QyujvItTP1lAnRujdxbJUT--z1sVzYg670LIo7R3KJ1aL3uj3TaYgL7BDqQFjfo9Wo4R1l5GswP0NSmA2xj1W7EDa9EH46xb71E7SrufZ92APrc37fXyGhG0mqIcY3iZPRukDfDqYT3Lvl98vOk-5VdfLpdde5UrykjMScO1HjDtcVkXWDJJS9UAZzAAY7RSTcPrQirNgQApqgIGSROCK17UAIUsz7L3B93t1G9AK0j2pRVbbzbS74WTRvz9MpofYu12oqo44zVOAu8eBLz7OUGIYmOCSj7lCG4KgjQp-qagjCf07T_orZt8SmGmeFkz1lQ0UecHSs1hexiOnyFYzNWLVL2YqxeH6tPEm8cejvyfrhPQHABISe4MeBGUgVGBNh5UFNqZ_4rfA79Gv6Y</recordid><startdate>20170901</startdate><enddate>20170901</enddate><creator>Takáč, Tomáš</creator><creator>Šamajová, Olga</creator><creator>Pechan, Tibor</creator><creator>Luptovčiak, Ivan</creator><creator>Šamaj, Jozef</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><general>The American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170901</creationdate><title>Feedback Microtubule Control and Microtubule-Actin Cross-talk in Arabidopsis Revealed by Integrative Proteomic and Cell Biology Analysis of KATANIN 1 Mutants</title><author>Takáč, Tomáš ; Šamajová, Olga ; Pechan, Tibor ; Luptovčiak, Ivan ; Šamaj, Jozef</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-198ddf04b03620a7a43c9e87efe7745c99862acd8e1e1252efa443c05826ee2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Actin</topic><topic>Actin-depolymerizing protein</topic><topic>Actins - metabolism</topic><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Cell Biology</topic><topic>Cell division</topic><topic>Control systems</topic><topic>Cytoskeleton</topic><topic>Deoxyribonucleic acid</topic><topic>Depolymerization</topic><topic>Deregulation</topic><topic>DNA</topic><topic>Elongation</topic><topic>Feedback</topic><topic>Feedback, Physiological</topic><topic>Filaments</topic><topic>Gene expression</topic><topic>Gene Ontology</topic><topic>Genes, Plant</topic><topic>Immunoblotting</topic><topic>Katanin - genetics</topic><topic>Metabolism</topic><topic>Microtubules</topic><topic>Microtubules - metabolism</topic><topic>Molecular Sequence Annotation</topic><topic>Morphogenesis</topic><topic>Mutants</topic><topic>Mutation - genetics</topic><topic>Phalloidin</topic><topic>Polymerase chain reaction</topic><topic>Profilin</topic><topic>Protein Interaction Maps</topic><topic>Proteins</topic><topic>Proteome - metabolism</topic><topic>Proteomics - methods</topic><topic>T-DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takáč, Tomáš</creatorcontrib><creatorcontrib>Šamajová, Olga</creatorcontrib><creatorcontrib>Pechan, Tibor</creatorcontrib><creatorcontrib>Luptovčiak, Ivan</creatorcontrib><creatorcontrib>Šamaj, Jozef</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular & cellular proteomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takáč, Tomáš</au><au>Šamajová, Olga</au><au>Pechan, Tibor</au><au>Luptovčiak, Ivan</au><au>Šamaj, Jozef</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Feedback Microtubule Control and Microtubule-Actin Cross-talk in Arabidopsis Revealed by Integrative Proteomic and Cell Biology Analysis of KATANIN 1 Mutants</atitle><jtitle>Molecular & cellular proteomics</jtitle><addtitle>Mol Cell Proteomics</addtitle><date>2017-09-01</date><risdate>2017</risdate><volume>16</volume><issue>9</issue><spage>1591</spage><epage>1609</epage><pages>1591-1609</pages><issn>1535-9476</issn><eissn>1535-9484</eissn><abstract>Microtubule organization and dynamics are critical for key developmental processes such as cell division, elongation, and morphogenesis. Microtubule severing is an essential regulator of microtubules and is exclusively executed by KATANIN 1 in Arabidopsis. In this study, we comparatively studied the proteome-wide effects in two KATANIN 1 mutants. Thus, shotgun proteomic analysis of roots and aerial parts of single nucleotide mutant fra2 and T-DNA insertion mutant ktn1-2 was carried out. We have detected 42 proteins differentially abundant in both fra2 and ktn1-2. KATANIN 1 dysfunction altered the abundance of proteins involved in development, metabolism, and stress responses. The differential regulation of tubulins and microtubule-destabilizing protein MDP25 implied a feedback microtubule control in KATANIN 1 mutants. Furthermore, deregulation of profilin 1, actin-depolymerizing factor 3, and actin 7 was observed. These findings were confirmed by immunoblotting analysis of actin and by microscopic observation of actin filaments using fluorescently labeled phalloidin. Results obtained by quantitative RT-PCR analysis revealed that changed protein abundances were not a consequence of altered expression levels of corresponding genes in the mutants. In conclusion, we show that abundances of several cytoskeletal proteins as well as organization of microtubules and the actin cytoskeleton are amended in accordance with defective microtubule severing.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>28706004</pmid><doi>10.1074/mcp.M117.068015</doi><tpages>19</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Actin Actin-depolymerizing protein Actins - metabolism Arabidopsis Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Cell Biology Cell division Control systems Cytoskeleton Deoxyribonucleic acid Depolymerization Deregulation DNA Elongation Feedback Feedback, Physiological Filaments Gene expression Gene Ontology Genes, Plant Immunoblotting Katanin - genetics Metabolism Microtubules Microtubules - metabolism Molecular Sequence Annotation Morphogenesis Mutants Mutation - genetics Phalloidin Polymerase chain reaction Profilin Protein Interaction Maps Proteins Proteome - metabolism Proteomics - methods T-DNA |
| title | Feedback Microtubule Control and Microtubule-Actin Cross-talk in Arabidopsis Revealed by Integrative Proteomic and Cell Biology Analysis of KATANIN 1 Mutants |
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