Endothelial Cell Culture Under Perfusion On A Polyester-Toner Microfluidic Device
This study presents an inexpensive and easy way to produce a microfluidic device that mimics a blood vessel, serving as a start point for cell culture under perfusion, cardiovascular research, and toxicological studies. Endpoint assays ( i.e ., MTT reduction and NO assays) were used and revealed tha...
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| Veröffentlicht in: | Scientific reports 2017-09, Vol.7 (1), p.10466-10466, Article 10466 |
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| creator | Urbaczek, Ana Carolina Leão, Paulo Augusto Gomes Carneiro Souza, Fayene Zeferino Ribeiro de Afonso, Ana Vieira Alberice, Juliana Cappelini, Luciana Teresa Dias Carlos, Iracilda Zeppone Carrilho, Emanuel |
| description | This study presents an inexpensive and easy way to produce a microfluidic device that mimics a blood vessel, serving as a start point for cell culture under perfusion, cardiovascular research, and toxicological studies. Endpoint assays (
i.e
., MTT reduction and NO assays) were used and revealed that the components making up the microchip, which is made of polyester and toner (PT), did not induce cell death or nitric oxide (NO) production. Applying oxygen plasma and fibronectin improved the adhesion and proliferation endothelial cell along the microchannel. As expected, these treatments showed an increase in vascular endothelial growth factor (VEGF-A) concentration profiles, which is correlated with adherence and cell proliferation, thus promoting endothelialization of the device for neovascularization. Regardless the simplicity of the device, our “vein-on-a-chip” mimetic has a potential to serve as a powerful tool for those that demand a rapid microfabrication method in cell biology or organ-on-a-chip research. |
| doi_str_mv | 10.1038/s41598-017-11043-0 |
| format | Article |
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., MTT reduction and NO assays) were used and revealed that the components making up the microchip, which is made of polyester and toner (PT), did not induce cell death or nitric oxide (NO) production. Applying oxygen plasma and fibronectin improved the adhesion and proliferation endothelial cell along the microchannel. As expected, these treatments showed an increase in vascular endothelial growth factor (VEGF-A) concentration profiles, which is correlated with adherence and cell proliferation, thus promoting endothelialization of the device for neovascularization. Regardless the simplicity of the device, our “vein-on-a-chip” mimetic has a potential to serve as a powerful tool for those that demand a rapid microfabrication method in cell biology or organ-on-a-chip research.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-017-11043-0</identifier><identifier>PMID: 28874818</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13 ; 13/107 ; 13/62 ; 631/80 ; 639/166/985 ; Biochips ; Cell culture ; Cell Culture Techniques ; Cell death ; Cell proliferation ; Endothelial cells ; Endothelial Cells - cytology ; Endothelial Cells - metabolism ; Fibronectin ; Human Umbilical Vein Endothelial Cells ; Humanities and Social Sciences ; Humans ; Lab-On-A-Chip Devices ; Microfluidic Analytical Techniques ; Microfluidics ; multidisciplinary ; Nitric oxide ; Nitric Oxide - metabolism ; Perfusion ; Polyesters ; Science ; Science (multidisciplinary) ; Toxicity testing ; Vascular endothelial growth factor ; Vascularization</subject><ispartof>Scientific reports, 2017-09, Vol.7 (1), p.10466-10466, Article 10466</ispartof><rights>The Author(s) 2017</rights><rights>Scientific Reports is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-b88596af41f35f37e35779e2da70af8a038ad065201f823c2fff524939047cb93</citedby><cites>FETCH-LOGICAL-c474t-b88596af41f35f37e35779e2da70af8a038ad065201f823c2fff524939047cb93</cites><orcidid>0000-0001-7351-8220</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5585355/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5585355/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28874818$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Urbaczek, Ana Carolina</creatorcontrib><creatorcontrib>Leão, Paulo Augusto Gomes Carneiro</creatorcontrib><creatorcontrib>Souza, Fayene Zeferino Ribeiro de</creatorcontrib><creatorcontrib>Afonso, Ana</creatorcontrib><creatorcontrib>Vieira Alberice, Juliana</creatorcontrib><creatorcontrib>Cappelini, Luciana Teresa Dias</creatorcontrib><creatorcontrib>Carlos, Iracilda Zeppone</creatorcontrib><creatorcontrib>Carrilho, Emanuel</creatorcontrib><title>Endothelial Cell Culture Under Perfusion On A Polyester-Toner Microfluidic Device</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>This study presents an inexpensive and easy way to produce a microfluidic device that mimics a blood vessel, serving as a start point for cell culture under perfusion, cardiovascular research, and toxicological studies. Endpoint assays (
i.e
., MTT reduction and NO assays) were used and revealed that the components making up the microchip, which is made of polyester and toner (PT), did not induce cell death or nitric oxide (NO) production. Applying oxygen plasma and fibronectin improved the adhesion and proliferation endothelial cell along the microchannel. As expected, these treatments showed an increase in vascular endothelial growth factor (VEGF-A) concentration profiles, which is correlated with adherence and cell proliferation, thus promoting endothelialization of the device for neovascularization. Regardless the simplicity of the device, our “vein-on-a-chip” mimetic has a potential to serve as a powerful tool for those that demand a rapid microfabrication method in cell biology or organ-on-a-chip research.</description><subject>13</subject><subject>13/107</subject><subject>13/62</subject><subject>631/80</subject><subject>639/166/985</subject><subject>Biochips</subject><subject>Cell culture</subject><subject>Cell Culture Techniques</subject><subject>Cell death</subject><subject>Cell proliferation</subject><subject>Endothelial cells</subject><subject>Endothelial Cells - cytology</subject><subject>Endothelial Cells - metabolism</subject><subject>Fibronectin</subject><subject>Human Umbilical Vein Endothelial Cells</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Lab-On-A-Chip Devices</subject><subject>Microfluidic Analytical Techniques</subject><subject>Microfluidics</subject><subject>multidisciplinary</subject><subject>Nitric oxide</subject><subject>Nitric Oxide - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Urbaczek, Ana Carolina</au><au>Leão, Paulo Augusto Gomes Carneiro</au><au>Souza, Fayene Zeferino Ribeiro de</au><au>Afonso, Ana</au><au>Vieira Alberice, Juliana</au><au>Cappelini, Luciana Teresa Dias</au><au>Carlos, Iracilda Zeppone</au><au>Carrilho, Emanuel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Endothelial Cell Culture Under Perfusion On A Polyester-Toner Microfluidic Device</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2017-09-05</date><risdate>2017</risdate><volume>7</volume><issue>1</issue><spage>10466</spage><epage>10466</epage><pages>10466-10466</pages><artnum>10466</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>This study presents an inexpensive and easy way to produce a microfluidic device that mimics a blood vessel, serving as a start point for cell culture under perfusion, cardiovascular research, and toxicological studies. Endpoint assays (
i.e
., MTT reduction and NO assays) were used and revealed that the components making up the microchip, which is made of polyester and toner (PT), did not induce cell death or nitric oxide (NO) production. Applying oxygen plasma and fibronectin improved the adhesion and proliferation endothelial cell along the microchannel. As expected, these treatments showed an increase in vascular endothelial growth factor (VEGF-A) concentration profiles, which is correlated with adherence and cell proliferation, thus promoting endothelialization of the device for neovascularization. Regardless the simplicity of the device, our “vein-on-a-chip” mimetic has a potential to serve as a powerful tool for those that demand a rapid microfabrication method in cell biology or organ-on-a-chip research.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>28874818</pmid><doi>10.1038/s41598-017-11043-0</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-7351-8220</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 13 13/107 13/62 631/80 639/166/985 Biochips Cell culture Cell Culture Techniques Cell death Cell proliferation Endothelial cells Endothelial Cells - cytology Endothelial Cells - metabolism Fibronectin Human Umbilical Vein Endothelial Cells Humanities and Social Sciences Humans Lab-On-A-Chip Devices Microfluidic Analytical Techniques Microfluidics multidisciplinary Nitric oxide Nitric Oxide - metabolism Perfusion Polyesters Science Science (multidisciplinary) Toxicity testing Vascular endothelial growth factor Vascularization |
| title | Endothelial Cell Culture Under Perfusion On A Polyester-Toner Microfluidic Device |
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