Remarkable plasticity of Na+, K+-ATPase, Ca2+-ATPase and SERCA contributes to muscle disuse atrophy resistance in hibernating Daurian ground squirrels
We investigated cytosolic calcium (Ca 2+ ) and sarcoplasmic reticulum Ca 2+ regulation in skeletal muscle fibers of hibernating Daurian ground squirrels ( Spermophilus dauricus ), non-hibernating hindlimb-unloaded (HLU) squirrels, and HLU rats to clarify the molecular mechanisms involved in preventi...
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| Veröffentlicht in: | Scientific reports 2017-09, Vol.7 (1), p.10509-10509, Article 10509 |
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| creator | Guo, Quanling Mi, Xin Sun, Xiaoyong Li, Xiaoyu Fu, Weiwei Xu, Shenhui Wang, Qi Arfat, Yasir Wang, Huiping Chang, Hui Gao, Yunfang |
| description | We investigated cytosolic calcium (Ca
2+
) and sarcoplasmic reticulum Ca
2+
regulation in skeletal muscle fibers of hibernating Daurian ground squirrels (
Spermophilus dauricus
), non-hibernating hindlimb-unloaded (HLU) squirrels, and HLU rats to clarify the molecular mechanisms involved in preventing muscle atrophy in hibernators. The Na
+
, K
+
-ATPase and Ca
2+
-ATPase activities in the soleus muscle (SOL) of squirrels were maintained in hibernation, decreased during interbout arousal (IB-A), and increased to autumn/pre-hibernation (AUT/Pre-H) levels in torpor after interbout arousal (Post-IBA), whereas activities in the extensor digitorum longus muscle (EDL) were stable during hibernation, but increased during post-hibernation (Post-H). Activities increased in the SOL of HLU rats, but were stable in HLU squirrels. Sarco/endoplasmic reticulum Ca
2+
-ATPase (SERCA) activity in the SOL decreased in IB-A squirrels, but returned to AUT/Pre-H levels in the Post-IBA group; no significant changes were found in the EDL. SERCA activity increased in the EDL of HLU squirrels and SOL of HLU rats. Compared with AUT/Pre-H, SERCA type 2 protein expression increased in the SOL and EDL of IB-A and Post-IBA squirrels, but increased in the SOL only in HLU animals. We also describe the protein kinase A changes in this paper. Thus, hibernating ground squirrels displayed remarkable Na
+
, K
+
-ATPase, Ca
2+
-ATPase, and SERCA plasticity. |
| doi_str_mv | 10.1038/s41598-017-10829-6 |
| format | Article |
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2+
) and sarcoplasmic reticulum Ca
2+
regulation in skeletal muscle fibers of hibernating Daurian ground squirrels (
Spermophilus dauricus
), non-hibernating hindlimb-unloaded (HLU) squirrels, and HLU rats to clarify the molecular mechanisms involved in preventing muscle atrophy in hibernators. The Na
+
, K
+
-ATPase and Ca
2+
-ATPase activities in the soleus muscle (SOL) of squirrels were maintained in hibernation, decreased during interbout arousal (IB-A), and increased to autumn/pre-hibernation (AUT/Pre-H) levels in torpor after interbout arousal (Post-IBA), whereas activities in the extensor digitorum longus muscle (EDL) were stable during hibernation, but increased during post-hibernation (Post-H). Activities increased in the SOL of HLU rats, but were stable in HLU squirrels. Sarco/endoplasmic reticulum Ca
2+
-ATPase (SERCA) activity in the SOL decreased in IB-A squirrels, but returned to AUT/Pre-H levels in the Post-IBA group; no significant changes were found in the EDL. SERCA activity increased in the EDL of HLU squirrels and SOL of HLU rats. Compared with AUT/Pre-H, SERCA type 2 protein expression increased in the SOL and EDL of IB-A and Post-IBA squirrels, but increased in the SOL only in HLU animals. We also describe the protein kinase A changes in this paper. Thus, hibernating ground squirrels displayed remarkable Na
+
, K
+
-ATPase, Ca
2+
-ATPase, and SERCA plasticity.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-017-10829-6</identifier><identifier>PMID: 28874726</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/337 ; 631/443 ; 82/1 ; 82/29 ; Humanities and Social Sciences ; multidisciplinary ; Science ; Science (multidisciplinary)</subject><ispartof>Scientific reports, 2017-09, Vol.7 (1), p.10509-10509, Article 10509</ispartof><rights>The Author(s) 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-ad462d60c88d44d0cb364f3733db5a8f96315b96d0c05e26ca6cf7068ba849593</citedby><cites>FETCH-LOGICAL-c353t-ad462d60c88d44d0cb364f3733db5a8f96315b96d0c05e26ca6cf7068ba849593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5585226/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5585226/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids></links><search><creatorcontrib>Guo, Quanling</creatorcontrib><creatorcontrib>Mi, Xin</creatorcontrib><creatorcontrib>Sun, Xiaoyong</creatorcontrib><creatorcontrib>Li, Xiaoyu</creatorcontrib><creatorcontrib>Fu, Weiwei</creatorcontrib><creatorcontrib>Xu, Shenhui</creatorcontrib><creatorcontrib>Wang, Qi</creatorcontrib><creatorcontrib>Arfat, Yasir</creatorcontrib><creatorcontrib>Wang, Huiping</creatorcontrib><creatorcontrib>Chang, Hui</creatorcontrib><creatorcontrib>Gao, Yunfang</creatorcontrib><title>Remarkable plasticity of Na+, K+-ATPase, Ca2+-ATPase and SERCA contributes to muscle disuse atrophy resistance in hibernating Daurian ground squirrels</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><description>We investigated cytosolic calcium (Ca
2+
) and sarcoplasmic reticulum Ca
2+
regulation in skeletal muscle fibers of hibernating Daurian ground squirrels (
Spermophilus dauricus
), non-hibernating hindlimb-unloaded (HLU) squirrels, and HLU rats to clarify the molecular mechanisms involved in preventing muscle atrophy in hibernators. The Na
+
, K
+
-ATPase and Ca
2+
-ATPase activities in the soleus muscle (SOL) of squirrels were maintained in hibernation, decreased during interbout arousal (IB-A), and increased to autumn/pre-hibernation (AUT/Pre-H) levels in torpor after interbout arousal (Post-IBA), whereas activities in the extensor digitorum longus muscle (EDL) were stable during hibernation, but increased during post-hibernation (Post-H). Activities increased in the SOL of HLU rats, but were stable in HLU squirrels. Sarco/endoplasmic reticulum Ca
2+
-ATPase (SERCA) activity in the SOL decreased in IB-A squirrels, but returned to AUT/Pre-H levels in the Post-IBA group; no significant changes were found in the EDL. SERCA activity increased in the EDL of HLU squirrels and SOL of HLU rats. Compared with AUT/Pre-H, SERCA type 2 protein expression increased in the SOL and EDL of IB-A and Post-IBA squirrels, but increased in the SOL only in HLU animals. We also describe the protein kinase A changes in this paper. Thus, hibernating ground squirrels displayed remarkable Na
+
, K
+
-ATPase, Ca
2+
-ATPase, and SERCA plasticity.</description><subject>631/337</subject><subject>631/443</subject><subject>82/1</subject><subject>82/29</subject><subject>Humanities and Social Sciences</subject><subject>multidisciplinary</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><recordid>eNp9kd9uFCEUh4nR2Kb2Bbzi0qQdZfg3zI3JZlttY6Om1mvCMMwudRa2HDDZF_F5Zd1q9EZugJzvfITzQ-hlS163hKk3wFvRq4a0XdMSRftGPkHHlHDRUEbp07_OR-gU4J7UJWjP2_45OqJKdbyj8hj9uHUbk76ZYXZ4OxvI3vq8w3HCH83ZOf5w1izuPhtw53hp6O8LNmHEXy5vlwtsY8jJDyU7wDniTQFbTaOHssdyitv1DicHHrIJ1mEf8NoPLgWTfVjhC1OSNwGvUizVCQ_Fp-RmeIGeTWYGd_q4n6Cv7y7vllfNzaf318vFTWOZYLkxI5d0lMQqNXI-EjswySfWMTYOwqipl6wVQy9rhQhHpTXSTh2RajCK96JnJ-jtwbstw8aN1tXfmFlvk69T2elovP63Evxar-J3LYQSlMoqePUoSPGhOMh648G6eTbBxQK67ZmsmKK8ovSA2hQBkpv-PNMSvc9UHzLVNVP9K1O997NDE1Q4rFzS97HU6c3wv66f1QSkng</recordid><startdate>20170905</startdate><enddate>20170905</enddate><creator>Guo, Quanling</creator><creator>Mi, Xin</creator><creator>Sun, Xiaoyong</creator><creator>Li, Xiaoyu</creator><creator>Fu, Weiwei</creator><creator>Xu, Shenhui</creator><creator>Wang, Qi</creator><creator>Arfat, Yasir</creator><creator>Wang, Huiping</creator><creator>Chang, Hui</creator><creator>Gao, Yunfang</creator><general>Nature Publishing Group UK</general><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20170905</creationdate><title>Remarkable plasticity of Na+, K+-ATPase, Ca2+-ATPase and SERCA contributes to muscle disuse atrophy resistance in hibernating Daurian ground squirrels</title><author>Guo, Quanling ; Mi, Xin ; Sun, Xiaoyong ; Li, Xiaoyu ; Fu, Weiwei ; Xu, Shenhui ; Wang, Qi ; Arfat, Yasir ; Wang, Huiping ; Chang, Hui ; Gao, Yunfang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-ad462d60c88d44d0cb364f3733db5a8f96315b96d0c05e26ca6cf7068ba849593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>631/337</topic><topic>631/443</topic><topic>82/1</topic><topic>82/29</topic><topic>Humanities and Social Sciences</topic><topic>multidisciplinary</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, Quanling</creatorcontrib><creatorcontrib>Mi, Xin</creatorcontrib><creatorcontrib>Sun, Xiaoyong</creatorcontrib><creatorcontrib>Li, Xiaoyu</creatorcontrib><creatorcontrib>Fu, Weiwei</creatorcontrib><creatorcontrib>Xu, Shenhui</creatorcontrib><creatorcontrib>Wang, Qi</creatorcontrib><creatorcontrib>Arfat, Yasir</creatorcontrib><creatorcontrib>Wang, Huiping</creatorcontrib><creatorcontrib>Chang, Hui</creatorcontrib><creatorcontrib>Gao, Yunfang</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, Quanling</au><au>Mi, Xin</au><au>Sun, Xiaoyong</au><au>Li, Xiaoyu</au><au>Fu, Weiwei</au><au>Xu, Shenhui</au><au>Wang, Qi</au><au>Arfat, Yasir</au><au>Wang, Huiping</au><au>Chang, Hui</au><au>Gao, Yunfang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Remarkable plasticity of Na+, K+-ATPase, Ca2+-ATPase and SERCA contributes to muscle disuse atrophy resistance in hibernating Daurian ground squirrels</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><date>2017-09-05</date><risdate>2017</risdate><volume>7</volume><issue>1</issue><spage>10509</spage><epage>10509</epage><pages>10509-10509</pages><artnum>10509</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>We investigated cytosolic calcium (Ca
2+
) and sarcoplasmic reticulum Ca
2+
regulation in skeletal muscle fibers of hibernating Daurian ground squirrels (
Spermophilus dauricus
), non-hibernating hindlimb-unloaded (HLU) squirrels, and HLU rats to clarify the molecular mechanisms involved in preventing muscle atrophy in hibernators. The Na
+
, K
+
-ATPase and Ca
2+
-ATPase activities in the soleus muscle (SOL) of squirrels were maintained in hibernation, decreased during interbout arousal (IB-A), and increased to autumn/pre-hibernation (AUT/Pre-H) levels in torpor after interbout arousal (Post-IBA), whereas activities in the extensor digitorum longus muscle (EDL) were stable during hibernation, but increased during post-hibernation (Post-H). Activities increased in the SOL of HLU rats, but were stable in HLU squirrels. Sarco/endoplasmic reticulum Ca
2+
-ATPase (SERCA) activity in the SOL decreased in IB-A squirrels, but returned to AUT/Pre-H levels in the Post-IBA group; no significant changes were found in the EDL. SERCA activity increased in the EDL of HLU squirrels and SOL of HLU rats. Compared with AUT/Pre-H, SERCA type 2 protein expression increased in the SOL and EDL of IB-A and Post-IBA squirrels, but increased in the SOL only in HLU animals. We also describe the protein kinase A changes in this paper. Thus, hibernating ground squirrels displayed remarkable Na
+
, K
+
-ATPase, Ca
2+
-ATPase, and SERCA plasticity.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>28874726</pmid><doi>10.1038/s41598-017-10829-6</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Scientific reports, 2017-09, Vol.7 (1), p.10509-10509, Article 10509 |
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| language | eng |
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| source | Nature Free; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; Springer Nature OA Free Journals |
| subjects | 631/337 631/443 82/1 82/29 Humanities and Social Sciences multidisciplinary Science Science (multidisciplinary) |
| title | Remarkable plasticity of Na+, K+-ATPase, Ca2+-ATPase and SERCA contributes to muscle disuse atrophy resistance in hibernating Daurian ground squirrels |
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