BMP7 dose‐dependently stimulates proliferation and cadherin‐11 expression via ERK and p38 in a murine metanephric mesenchymal cell line
BMP7 is expressed in ureteric buds and cap mesenchyme of the fetal kidney, mediating branching morphogenesis and survival and priming of metanephric mesenchyme. Although dose‐dependent effects of BMP7 in collecting duct cells have been reported, studies in metanephric mesenchymal cells are lacking....
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| description | BMP7 is expressed in ureteric buds and cap mesenchyme of the fetal kidney, mediating branching morphogenesis and survival and priming of metanephric mesenchyme. Although dose‐dependent effects of BMP7 in collecting duct cells have been reported, studies in metanephric mesenchymal cells are lacking. We examined the effects of BMP7 on MAP kinase activation, proliferation, and expression of cadherins in a metanephric mesenchymal cell line MS7 by thymidine incorporation, immunoblot analysis, and quantitative real‐time PCR. The levels of phosphorylated ERK (P‐ERK) and phosphorylated p38 (P‐p38) were not altered at 10 min, 1 h, and 6 h with low‐dose BMP7 (0.25 nmol/L), but were increased at 24 h. At 24 h, P‐ERK was increased with low‐dose BMP7, but not by intermediate‐ (1 nmol/L) or high‐dose (10 nmol/L) BMP7, whereas p38 was activated by intermediate‐dose BMP7. Cell proliferation of MS7 was significantly increased by low‐ and intermediate‐dose BMP7 and decreased by high‐dose BMP7. A p38 inhibitor SB203580 5 μmol/L or a MEK inhibitor PD98059 5 μmol/L abolished BMP7‐stimulated proliferation. Expression of cadherin‐11, an adhesion molecule known to promote cell migration and compaction, was upregulated by intermediate‐dose BMP7. BMP7‐induced cadherin‐11 expression was inhibited by cotreatment with SB203580 and PD98059. Finally, in metanephroi cultured with siRNA for cadherin‐11, the number and thickness of cap mesenchyme were reduced. In conclusion, BMP7 exerts differential effects depending on the concentration; it may expand mesenchymal cells in the stroma where BMP7 concentration is low and may upregulate cadherin‐11 promoting condensation around the tip of ureteric buds.
BMP7 exerts differential effects depending on the concentration in the developing kidney. It may expand mesenchymal cells in the stroma where BMP7 concentration is low and may upregulate cadherin‐11 promoting condensation around the tip of ureteric buds. |
| doi_str_mv | 10.14814/phy2.13378 |
| format | Article |
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BMP7 exerts differential effects depending on the concentration in the developing kidney. It may expand mesenchymal cells in the stroma where BMP7 concentration is low and may upregulate cadherin‐11 promoting condensation around the tip of ureteric buds.</description><identifier>EISSN: 2051-817X</identifier><identifier>DOI: 10.14814/phy2.13378</identifier><identifier>PMID: 28867673</identifier><language>eng</language><publisher>United States: John Wiley & Sons, Inc</publisher><subject>Animals ; Bone morphogenetic protein 7 ; Bone Morphogenetic Protein 7 - administration & dosage ; Bone Morphogenetic Protein 7 - metabolism ; Cadherins ; Cadherins - metabolism ; cap mesenchyme ; Cell adhesion & migration ; Cell Line ; Cell migration ; Cell Proliferation ; Collecting duct ; Compaction ; development ; Development and Regeneration ; Extracellular signal-regulated kinase ; Fetuses ; Kidney ; Kidney - physiology ; Kidneys ; MAP kinase ; Mesenchyme ; Mice ; Mitogen-Activated Protein Kinase 3 - metabolism ; mitogen‐activated protein kinase ; Morphogenesis ; Organ Culture Techniques ; Original Research ; p38 Mitogen-Activated Protein Kinases - metabolism ; Physiology ; Regulatory Pathways ; Signalling Pathways ; siRNA ; Stroma ; Thymidine ; Ureter</subject><ispartof>Physiological reports, 2017-08, Vol.5 (16), p.e13378-n/a</ispartof><rights>2017 The Authors. published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society</rights><rights>2017 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.</rights><rights>2017. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5908-b71fe8de9ec6b1d9e18ba8eb844a526fa08097ecda1a6a51445983a9703cc7753</citedby><cites>FETCH-LOGICAL-c5908-b71fe8de9ec6b1d9e18ba8eb844a526fa08097ecda1a6a51445983a9703cc7753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5582263/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5582263/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,1417,11562,27924,27925,45574,45575,46052,46476,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28867673$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Awazu, Midori</creatorcontrib><creatorcontrib>Nagata, Michio</creatorcontrib><creatorcontrib>Hida, Mariko</creatorcontrib><title>BMP7 dose‐dependently stimulates proliferation and cadherin‐11 expression via ERK and p38 in a murine metanephric mesenchymal cell line</title><title>Physiological reports</title><addtitle>Physiol Rep</addtitle><description>BMP7 is expressed in ureteric buds and cap mesenchyme of the fetal kidney, mediating branching morphogenesis and survival and priming of metanephric mesenchyme. Although dose‐dependent effects of BMP7 in collecting duct cells have been reported, studies in metanephric mesenchymal cells are lacking. We examined the effects of BMP7 on MAP kinase activation, proliferation, and expression of cadherins in a metanephric mesenchymal cell line MS7 by thymidine incorporation, immunoblot analysis, and quantitative real‐time PCR. The levels of phosphorylated ERK (P‐ERK) and phosphorylated p38 (P‐p38) were not altered at 10 min, 1 h, and 6 h with low‐dose BMP7 (0.25 nmol/L), but were increased at 24 h. At 24 h, P‐ERK was increased with low‐dose BMP7, but not by intermediate‐ (1 nmol/L) or high‐dose (10 nmol/L) BMP7, whereas p38 was activated by intermediate‐dose BMP7. Cell proliferation of MS7 was significantly increased by low‐ and intermediate‐dose BMP7 and decreased by high‐dose BMP7. A p38 inhibitor SB203580 5 μmol/L or a MEK inhibitor PD98059 5 μmol/L abolished BMP7‐stimulated proliferation. Expression of cadherin‐11, an adhesion molecule known to promote cell migration and compaction, was upregulated by intermediate‐dose BMP7. BMP7‐induced cadherin‐11 expression was inhibited by cotreatment with SB203580 and PD98059. Finally, in metanephroi cultured with siRNA for cadherin‐11, the number and thickness of cap mesenchyme were reduced. In conclusion, BMP7 exerts differential effects depending on the concentration; it may expand mesenchymal cells in the stroma where BMP7 concentration is low and may upregulate cadherin‐11 promoting condensation around the tip of ureteric buds.
BMP7 exerts differential effects depending on the concentration in the developing kidney. It may expand mesenchymal cells in the stroma where BMP7 concentration is low and may upregulate cadherin‐11 promoting condensation around the tip of ureteric buds.</description><subject>Animals</subject><subject>Bone morphogenetic protein 7</subject><subject>Bone Morphogenetic Protein 7 - administration & dosage</subject><subject>Bone Morphogenetic Protein 7 - metabolism</subject><subject>Cadherins</subject><subject>Cadherins - metabolism</subject><subject>cap mesenchyme</subject><subject>Cell adhesion & migration</subject><subject>Cell Line</subject><subject>Cell migration</subject><subject>Cell Proliferation</subject><subject>Collecting duct</subject><subject>Compaction</subject><subject>development</subject><subject>Development and Regeneration</subject><subject>Extracellular signal-regulated kinase</subject><subject>Fetuses</subject><subject>Kidney</subject><subject>Kidney - physiology</subject><subject>Kidneys</subject><subject>MAP kinase</subject><subject>Mesenchyme</subject><subject>Mice</subject><subject>Mitogen-Activated Protein Kinase 3 - metabolism</subject><subject>mitogen‐activated protein kinase</subject><subject>Morphogenesis</subject><subject>Organ Culture Techniques</subject><subject>Original Research</subject><subject>p38 Mitogen-Activated Protein Kinases - metabolism</subject><subject>Physiology</subject><subject>Regulatory Pathways</subject><subject>Signalling Pathways</subject><subject>siRNA</subject><subject>Stroma</subject><subject>Thymidine</subject><subject>Ureter</subject><issn>2051-817X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU1rFTEUhgdBbGm7ci8BN4LcNh-TSWYjaKmttGIRBV2FTHLGSclMxmSmOjv3bvyN_SXNvbcWdeEqgfOch_PyFsVjgg9JKUl5NHYLPSSMCfmg2KWYk5Uk4tNOcZDSFcaYYMZqXD4qdqiUlagE2y1-vnp7KZANCW5-_LIwwmBhmPyC0uT62esJEhpj8K6FqCcXBqQHi4y2HUQ35B1CEHwfI6S0Hl47jU7en2-gkUnkMo_6OaOAepj0AGMXncn_BIPpll57ZMB75DOxXzxstU9wcPfuFR9fn3w4PltdvDt9c_zyYmV4jeWqEaQFaaEGUzXE1kBkoyU0siw1p1WrscS1AGM10ZXmpCx5LZmuBWbGCMHZXvFi6x3npgdrcuCovRqj63VcVNBO_T0ZXKe-hGvFuaS0Ylnw7E4Qw9cZ0qR6l9Yxcr4wJ0VqxllNsKQZffoPehXmOOR4meJMckzxWvh8S5kYUorQ3h9DsNp0q9bdqk23mX7y5_337O9WM0C3wDfnYfmfS12efaZb6y3EY7UT</recordid><startdate>201708</startdate><enddate>201708</enddate><creator>Awazu, Midori</creator><creator>Nagata, Michio</creator><creator>Hida, Mariko</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201708</creationdate><title>BMP7 dose‐dependently stimulates proliferation and cadherin‐11 expression via ERK and p38 in a murine metanephric mesenchymal cell line</title><author>Awazu, Midori ; Nagata, Michio ; Hida, Mariko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5908-b71fe8de9ec6b1d9e18ba8eb844a526fa08097ecda1a6a51445983a9703cc7753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animals</topic><topic>Bone morphogenetic protein 7</topic><topic>Bone Morphogenetic Protein 7 - administration & dosage</topic><topic>Bone Morphogenetic Protein 7 - metabolism</topic><topic>Cadherins</topic><topic>Cadherins - metabolism</topic><topic>cap mesenchyme</topic><topic>Cell adhesion & migration</topic><topic>Cell Line</topic><topic>Cell migration</topic><topic>Cell Proliferation</topic><topic>Collecting duct</topic><topic>Compaction</topic><topic>development</topic><topic>Development and Regeneration</topic><topic>Extracellular signal-regulated kinase</topic><topic>Fetuses</topic><topic>Kidney</topic><topic>Kidney - physiology</topic><topic>Kidneys</topic><topic>MAP kinase</topic><topic>Mesenchyme</topic><topic>Mice</topic><topic>Mitogen-Activated Protein Kinase 3 - metabolism</topic><topic>mitogen‐activated protein kinase</topic><topic>Morphogenesis</topic><topic>Organ Culture Techniques</topic><topic>Original Research</topic><topic>p38 Mitogen-Activated Protein Kinases - metabolism</topic><topic>Physiology</topic><topic>Regulatory Pathways</topic><topic>Signalling Pathways</topic><topic>siRNA</topic><topic>Stroma</topic><topic>Thymidine</topic><topic>Ureter</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Awazu, Midori</creatorcontrib><creatorcontrib>Nagata, Michio</creatorcontrib><creatorcontrib>Hida, Mariko</creatorcontrib><collection>Wiley-Blackwell Open Access Titles</collection><collection>Wiley Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Physiological reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Awazu, Midori</au><au>Nagata, Michio</au><au>Hida, Mariko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>BMP7 dose‐dependently stimulates proliferation and cadherin‐11 expression via ERK and p38 in a murine metanephric mesenchymal cell line</atitle><jtitle>Physiological reports</jtitle><addtitle>Physiol Rep</addtitle><date>2017-08</date><risdate>2017</risdate><volume>5</volume><issue>16</issue><spage>e13378</spage><epage>n/a</epage><pages>e13378-n/a</pages><eissn>2051-817X</eissn><abstract>BMP7 is expressed in ureteric buds and cap mesenchyme of the fetal kidney, mediating branching morphogenesis and survival and priming of metanephric mesenchyme. Although dose‐dependent effects of BMP7 in collecting duct cells have been reported, studies in metanephric mesenchymal cells are lacking. We examined the effects of BMP7 on MAP kinase activation, proliferation, and expression of cadherins in a metanephric mesenchymal cell line MS7 by thymidine incorporation, immunoblot analysis, and quantitative real‐time PCR. The levels of phosphorylated ERK (P‐ERK) and phosphorylated p38 (P‐p38) were not altered at 10 min, 1 h, and 6 h with low‐dose BMP7 (0.25 nmol/L), but were increased at 24 h. At 24 h, P‐ERK was increased with low‐dose BMP7, but not by intermediate‐ (1 nmol/L) or high‐dose (10 nmol/L) BMP7, whereas p38 was activated by intermediate‐dose BMP7. Cell proliferation of MS7 was significantly increased by low‐ and intermediate‐dose BMP7 and decreased by high‐dose BMP7. A p38 inhibitor SB203580 5 μmol/L or a MEK inhibitor PD98059 5 μmol/L abolished BMP7‐stimulated proliferation. Expression of cadherin‐11, an adhesion molecule known to promote cell migration and compaction, was upregulated by intermediate‐dose BMP7. BMP7‐induced cadherin‐11 expression was inhibited by cotreatment with SB203580 and PD98059. Finally, in metanephroi cultured with siRNA for cadherin‐11, the number and thickness of cap mesenchyme were reduced. In conclusion, BMP7 exerts differential effects depending on the concentration; it may expand mesenchymal cells in the stroma where BMP7 concentration is low and may upregulate cadherin‐11 promoting condensation around the tip of ureteric buds.
BMP7 exerts differential effects depending on the concentration in the developing kidney. It may expand mesenchymal cells in the stroma where BMP7 concentration is low and may upregulate cadherin‐11 promoting condensation around the tip of ureteric buds.</abstract><cop>United States</cop><pub>John Wiley & Sons, Inc</pub><pmid>28867673</pmid><doi>10.14814/phy2.13378</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Bone morphogenetic protein 7 Bone Morphogenetic Protein 7 - administration & dosage Bone Morphogenetic Protein 7 - metabolism Cadherins Cadherins - metabolism cap mesenchyme Cell adhesion & migration Cell Line Cell migration Cell Proliferation Collecting duct Compaction development Development and Regeneration Extracellular signal-regulated kinase Fetuses Kidney Kidney - physiology Kidneys MAP kinase Mesenchyme Mice Mitogen-Activated Protein Kinase 3 - metabolism mitogen‐activated protein kinase Morphogenesis Organ Culture Techniques Original Research p38 Mitogen-Activated Protein Kinases - metabolism Physiology Regulatory Pathways Signalling Pathways siRNA Stroma Thymidine Ureter |
| title | BMP7 dose‐dependently stimulates proliferation and cadherin‐11 expression via ERK and p38 in a murine metanephric mesenchymal cell line |
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