Generating high-purity cardiac and endothelial derivatives from patterned mesoderm using human pluripotent stem cells

Different concentrations of activin A and BMP4 are used to polarize stem cells into mesodermal subtypes that reflect mid-primitive-streak cardiogenic mesoderm and posterior-primitive-streak hemogenic mesoderm. Human pluripotent stem cells (hPSCs) provide a valuable model for the study of human devel...

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Veröffentlicht in:	Nature protocols 2017-01, Vol.12 (1), p.15-31
Hauptverfasser:	Palpant, Nathan J, Pabon, Lil, Friedman, Clayton E, Roberts, Meredith, Hadland, Brandon, Zaunbrecher, Rebecca J, Bernstein, Irwin, Zheng, Ying, Murry, Charles E
Format:	Artikel
Sprache:	eng
Schlagworte:	631/136/532/2064 631/1647/1407/651 631/443/592/2726 631/532/1360 Activin Analytical Chemistry Bioengineering Biological Techniques Bone morphogenetic protein 4 Cadherins Cardiomyocytes Cell culture Cell Culture Techniques - methods Cell Differentiation Cell fate Cell Line Cell Lineage Computational Biology/Bioinformatics Derivation Differentiation Endothelial cells Endothelial Cells - cytology Endothelium Growth Heart Humans Innovations Life Sciences Medical research Medicine Mesoderm Mesoderm - cytology Microarrays Myocardium - cytology Organic Chemistry Pluripotency Pluripotent Stem Cells - cytology Primitive streak Protocol Stem cell research Stem cells Therapeutic applications
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