Single-Cell Phosphospecific Flow Cytometric Analysis of Canine and Murine Adipose-Derived Stem Cells

Single-Cell Phosphospecific Flow Cytometric Analysis of Canine and Murine Adipose-Derived Stem Cells

This study aimed to demonstrate single-cell phosphospecific flow cytometric analysis of canine and murine adipose-derived stem/stromal cells (ADSCs). ADSCs were obtained from clinically healthy laboratory beagles and C57BL/6 mice. Cell differentiation into adipocytes, osteocytes, and chondrocytes wa...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of veterinary medicine 2017-01, Vol.2017, p.5701016-7
Hauptverfasser: Itoh, Harumichi, Nishikawa, Shimpei, Haraguchi, Tomoya, Arikawa, Yu, Hiyama, Masato, Iseri, Toshie, Itoh, Yoshiki, Nakaichi, Munekazu, Taura, Yasuho, Tani, Kenji, Itamoto, Kazuhito
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 7
container_issue
container_start_page 5701016
container_title Journal of veterinary medicine
container_volume 2017
creator Itoh, Harumichi
Nishikawa, Shimpei
Haraguchi, Tomoya
Arikawa, Yu
Hiyama, Masato
Iseri, Toshie
Itoh, Yoshiki
Nakaichi, Munekazu
Taura, Yasuho
Tani, Kenji
Itamoto, Kazuhito
description This study aimed to demonstrate single-cell phosphospecific flow cytometric analysis of canine and murine adipose-derived stem/stromal cells (ADSCs). ADSCs were obtained from clinically healthy laboratory beagles and C57BL/6 mice. Cell differentiation into adipocytes, osteocytes, and chondrocytes was observed for the cultured canine ADSCs (cADSCs) and murine ADSCs (mADSCs) to determine their multipotency. We also performed single-cell phosphospecific flow cytometric analysis related to cell differentiation and stemness. Cultured cADSCs and mADSCs exhibited the potential to differentiate into adipocytes, osteocytes, and chondrocytes. In addition, single-cell phosphospecific flow cytometric analysis revealed similar β-catenin and Akt phosphorylation between mADSCs and cADSCs. On the other hand, it showed the phosphorylation of different Stat proteins. It was determined that cADSCs and mADSCs show the potential to differentiate into adipocytes, osteocytes, and chondrocytes. Furthermore, a difference in protein phosphorylation between undifferentiated cADSCs and mADSCs was identified.
doi_str_mv 10.1155/2017/5701016
format Article
fullrecord <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5576408</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>28900631</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3986-5c3b1f6a31e162c0e411d12179a21a47be90140bb46651d7e1b67da3db28c7803</originalsourceid><addsrcrecordid>eNp9kEFLw0AQhRdRbKm9eZa9a-xONtlNLkKJVoWKQvUcNtlJu5JsQra29N-b0Fr04mGYN8ybb-ARcgnsFiAMJz4DOQklAwbihAx9DoEnYiFOex0KT0oWDcjYuU_GGACPOffPycCPYsYEhyHRC2OXJXoJliV9W9Wu6QtzU5iczsp6S5Pduq5w3Xbz1Kpy54yjdUETZY1FqqymL19tL6faNLVD7x5bs0FNF2usaM91F-SsUKXD8aGPyMfs4T158uavj8_JdO7lPI6EF-Y8g0IoDgjCzxkGABp8kLHyQQUyw5hBwLIsECIELREyIbXiOvOjXEaMj8jdntt8ZRXqHO26VWXatKZS7S6tlUn_bqxZpct6k4ahFAGLOsDNHpC3tXMtFsdbYGkfeNoHnh4C7-xXv_8dzT_xdobrvWFlrFZb8z_uGwERiJ0</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Single-Cell Phosphospecific Flow Cytometric Analysis of Canine and Murine Adipose-Derived Stem Cells</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>Wiley-Blackwell Open Access Titles</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Itoh, Harumichi ; Nishikawa, Shimpei ; Haraguchi, Tomoya ; Arikawa, Yu ; Hiyama, Masato ; Iseri, Toshie ; Itoh, Yoshiki ; Nakaichi, Munekazu ; Taura, Yasuho ; Tani, Kenji ; Itamoto, Kazuhito</creator><contributor>Tohno, Masanori</contributor><creatorcontrib>Itoh, Harumichi ; Nishikawa, Shimpei ; Haraguchi, Tomoya ; Arikawa, Yu ; Hiyama, Masato ; Iseri, Toshie ; Itoh, Yoshiki ; Nakaichi, Munekazu ; Taura, Yasuho ; Tani, Kenji ; Itamoto, Kazuhito ; Tohno, Masanori</creatorcontrib><description>This study aimed to demonstrate single-cell phosphospecific flow cytometric analysis of canine and murine adipose-derived stem/stromal cells (ADSCs). ADSCs were obtained from clinically healthy laboratory beagles and C57BL/6 mice. Cell differentiation into adipocytes, osteocytes, and chondrocytes was observed for the cultured canine ADSCs (cADSCs) and murine ADSCs (mADSCs) to determine their multipotency. We also performed single-cell phosphospecific flow cytometric analysis related to cell differentiation and stemness. Cultured cADSCs and mADSCs exhibited the potential to differentiate into adipocytes, osteocytes, and chondrocytes. In addition, single-cell phosphospecific flow cytometric analysis revealed similar β-catenin and Akt phosphorylation between mADSCs and cADSCs. On the other hand, it showed the phosphorylation of different Stat proteins. It was determined that cADSCs and mADSCs show the potential to differentiate into adipocytes, osteocytes, and chondrocytes. Furthermore, a difference in protein phosphorylation between undifferentiated cADSCs and mADSCs was identified.</description><identifier>ISSN: 2356-7708</identifier><identifier>EISSN: 2314-6966</identifier><identifier>DOI: 10.1155/2017/5701016</identifier><identifier>PMID: 28900631</identifier><language>eng</language><publisher>United States: Hindawi</publisher><ispartof>Journal of veterinary medicine, 2017-01, Vol.2017, p.5701016-7</ispartof><rights>Copyright © 2017 Harumichi Itoh et al.</rights><rights>Copyright © 2017 Harumichi Itoh et al. 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3986-5c3b1f6a31e162c0e411d12179a21a47be90140bb46651d7e1b67da3db28c7803</citedby><cites>FETCH-LOGICAL-c3986-5c3b1f6a31e162c0e411d12179a21a47be90140bb46651d7e1b67da3db28c7803</cites><orcidid>0000-0003-1957-0129 ; 0000-0002-7788-9376</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5576408/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5576408/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,886,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28900631$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Tohno, Masanori</contributor><creatorcontrib>Itoh, Harumichi</creatorcontrib><creatorcontrib>Nishikawa, Shimpei</creatorcontrib><creatorcontrib>Haraguchi, Tomoya</creatorcontrib><creatorcontrib>Arikawa, Yu</creatorcontrib><creatorcontrib>Hiyama, Masato</creatorcontrib><creatorcontrib>Iseri, Toshie</creatorcontrib><creatorcontrib>Itoh, Yoshiki</creatorcontrib><creatorcontrib>Nakaichi, Munekazu</creatorcontrib><creatorcontrib>Taura, Yasuho</creatorcontrib><creatorcontrib>Tani, Kenji</creatorcontrib><creatorcontrib>Itamoto, Kazuhito</creatorcontrib><title>Single-Cell Phosphospecific Flow Cytometric Analysis of Canine and Murine Adipose-Derived Stem Cells</title><title>Journal of veterinary medicine</title><addtitle>J Vet Med</addtitle><description>This study aimed to demonstrate single-cell phosphospecific flow cytometric analysis of canine and murine adipose-derived stem/stromal cells (ADSCs). ADSCs were obtained from clinically healthy laboratory beagles and C57BL/6 mice. Cell differentiation into adipocytes, osteocytes, and chondrocytes was observed for the cultured canine ADSCs (cADSCs) and murine ADSCs (mADSCs) to determine their multipotency. We also performed single-cell phosphospecific flow cytometric analysis related to cell differentiation and stemness. Cultured cADSCs and mADSCs exhibited the potential to differentiate into adipocytes, osteocytes, and chondrocytes. In addition, single-cell phosphospecific flow cytometric analysis revealed similar β-catenin and Akt phosphorylation between mADSCs and cADSCs. On the other hand, it showed the phosphorylation of different Stat proteins. It was determined that cADSCs and mADSCs show the potential to differentiate into adipocytes, osteocytes, and chondrocytes. Furthermore, a difference in protein phosphorylation between undifferentiated cADSCs and mADSCs was identified.</description><issn>2356-7708</issn><issn>2314-6966</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><recordid>eNp9kEFLw0AQhRdRbKm9eZa9a-xONtlNLkKJVoWKQvUcNtlJu5JsQra29N-b0Fr04mGYN8ybb-ARcgnsFiAMJz4DOQklAwbihAx9DoEnYiFOex0KT0oWDcjYuU_GGACPOffPycCPYsYEhyHRC2OXJXoJliV9W9Wu6QtzU5iczsp6S5Pduq5w3Xbz1Kpy54yjdUETZY1FqqymL19tL6faNLVD7x5bs0FNF2usaM91F-SsUKXD8aGPyMfs4T158uavj8_JdO7lPI6EF-Y8g0IoDgjCzxkGABp8kLHyQQUyw5hBwLIsECIELREyIbXiOvOjXEaMj8jdntt8ZRXqHO26VWXatKZS7S6tlUn_bqxZpct6k4ahFAGLOsDNHpC3tXMtFsdbYGkfeNoHnh4C7-xXv_8dzT_xdobrvWFlrFZb8z_uGwERiJ0</recordid><startdate>20170101</startdate><enddate>20170101</enddate><creator>Itoh, Harumichi</creator><creator>Nishikawa, Shimpei</creator><creator>Haraguchi, Tomoya</creator><creator>Arikawa, Yu</creator><creator>Hiyama, Masato</creator><creator>Iseri, Toshie</creator><creator>Itoh, Yoshiki</creator><creator>Nakaichi, Munekazu</creator><creator>Taura, Yasuho</creator><creator>Tani, Kenji</creator><creator>Itamoto, Kazuhito</creator><general>Hindawi</general><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1957-0129</orcidid><orcidid>https://orcid.org/0000-0002-7788-9376</orcidid></search><sort><creationdate>20170101</creationdate><title>Single-Cell Phosphospecific Flow Cytometric Analysis of Canine and Murine Adipose-Derived Stem Cells</title><author>Itoh, Harumichi ; Nishikawa, Shimpei ; Haraguchi, Tomoya ; Arikawa, Yu ; Hiyama, Masato ; Iseri, Toshie ; Itoh, Yoshiki ; Nakaichi, Munekazu ; Taura, Yasuho ; Tani, Kenji ; Itamoto, Kazuhito</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3986-5c3b1f6a31e162c0e411d12179a21a47be90140bb46651d7e1b67da3db28c7803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Itoh, Harumichi</creatorcontrib><creatorcontrib>Nishikawa, Shimpei</creatorcontrib><creatorcontrib>Haraguchi, Tomoya</creatorcontrib><creatorcontrib>Arikawa, Yu</creatorcontrib><creatorcontrib>Hiyama, Masato</creatorcontrib><creatorcontrib>Iseri, Toshie</creatorcontrib><creatorcontrib>Itoh, Yoshiki</creatorcontrib><creatorcontrib>Nakaichi, Munekazu</creatorcontrib><creatorcontrib>Taura, Yasuho</creatorcontrib><creatorcontrib>Tani, Kenji</creatorcontrib><creatorcontrib>Itamoto, Kazuhito</creatorcontrib><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of veterinary medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Itoh, Harumichi</au><au>Nishikawa, Shimpei</au><au>Haraguchi, Tomoya</au><au>Arikawa, Yu</au><au>Hiyama, Masato</au><au>Iseri, Toshie</au><au>Itoh, Yoshiki</au><au>Nakaichi, Munekazu</au><au>Taura, Yasuho</au><au>Tani, Kenji</au><au>Itamoto, Kazuhito</au><au>Tohno, Masanori</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Single-Cell Phosphospecific Flow Cytometric Analysis of Canine and Murine Adipose-Derived Stem Cells</atitle><jtitle>Journal of veterinary medicine</jtitle><addtitle>J Vet Med</addtitle><date>2017-01-01</date><risdate>2017</risdate><volume>2017</volume><spage>5701016</spage><epage>7</epage><pages>5701016-7</pages><issn>2356-7708</issn><eissn>2314-6966</eissn><abstract>This study aimed to demonstrate single-cell phosphospecific flow cytometric analysis of canine and murine adipose-derived stem/stromal cells (ADSCs). ADSCs were obtained from clinically healthy laboratory beagles and C57BL/6 mice. Cell differentiation into adipocytes, osteocytes, and chondrocytes was observed for the cultured canine ADSCs (cADSCs) and murine ADSCs (mADSCs) to determine their multipotency. We also performed single-cell phosphospecific flow cytometric analysis related to cell differentiation and stemness. Cultured cADSCs and mADSCs exhibited the potential to differentiate into adipocytes, osteocytes, and chondrocytes. In addition, single-cell phosphospecific flow cytometric analysis revealed similar β-catenin and Akt phosphorylation between mADSCs and cADSCs. On the other hand, it showed the phosphorylation of different Stat proteins. It was determined that cADSCs and mADSCs show the potential to differentiate into adipocytes, osteocytes, and chondrocytes. Furthermore, a difference in protein phosphorylation between undifferentiated cADSCs and mADSCs was identified.</abstract><cop>United States</cop><pub>Hindawi</pub><pmid>28900631</pmid><doi>10.1155/2017/5701016</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-1957-0129</orcidid><orcidid>https://orcid.org/0000-0002-7788-9376</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2356-7708
ispartof Journal of veterinary medicine, 2017-01, Vol.2017, p.5701016-7
issn 2356-7708
2314-6966
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5576408
source Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Wiley-Blackwell Open Access Titles; PubMed Central; Alma/SFX Local Collection
title Single-Cell Phosphospecific Flow Cytometric Analysis of Canine and Murine Adipose-Derived Stem Cells
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-15T14%3A40%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Single-Cell%20Phosphospecific%20Flow%20Cytometric%20Analysis%20of%20Canine%20and%20Murine%20Adipose-Derived%20Stem%20Cells&rft.jtitle=Journal%20of%20veterinary%20medicine&rft.au=Itoh,%20Harumichi&rft.date=2017-01-01&rft.volume=2017&rft.spage=5701016&rft.epage=7&rft.pages=5701016-7&rft.issn=2356-7708&rft.eissn=2314-6966&rft_id=info:doi/10.1155/2017/5701016&rft_dat=%3Cpubmed_cross%3E28900631%3C/pubmed_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/28900631&rfr_iscdi=true