A new G-tailing method for the determination of the poly(A) tail length applied to hepatitis A virus RNA

To study the role of the poly(A) tail length during the replication of poly(A)-containing plus-strand RNA virus, we have developed a simple reverse transcription polymerase chain reaction (RT-PCR)-based method that substantially improves the previously reported PAT [poly(A) test] assay. In contrast...

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Veröffentlicht in:	Nucleic acids research 2001-06, Vol.29 (12), p.E57-57
Hauptverfasser:	Kusov, Y Y, Shatirishvili, G, Dzagurov, G, Gauss-Müller, V
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine - genetics Adenosine - metabolism Animals Base Sequence Cell Line DNA Primers - genetics Genome, Viral Guanine - metabolism Hepatitis A virus Hepatovirus - genetics Hepatovirus - growth & development Humans NAR Methods Online Oligodeoxyribonucleotides - genetics Poly A - analysis Poly A - chemistry Poly A - genetics Poly G - biosynthesis Poly G - genetics Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis RNA, Messenger - chemistry RNA, Messenger - genetics RNA, Viral - analysis RNA, Viral - chemistry RNA, Viral - genetics Sensitivity and Specificity Sequence Analysis, DNA Transfection Virus Replication - genetics
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