cDNA clones for the ecdysone-inducible polypeptide (EIP) mRNAs of Drosophila Kc cells
The ecdysone‐inducible polypeptides (EIPs) 28, 29 and 40 were identified previously as polypeptides whose synthesis is stimulated early in the ecdysone response of Drosophila Kc cells. We have now shown, using two‐dimensional gels, that each of these EIPs consists of three species differing in pI, a...
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| Veröffentlicht in: | The EMBO journal 1984, Vol.3 (1), p.235-243 |
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| description | The ecdysone‐inducible polypeptides (EIPs) 28, 29 and 40 were identified previously as polypeptides whose synthesis is stimulated early in the ecdysone response of Drosophila Kc cells. We have now shown, using two‐dimensional gels, that each of these EIPs consists of three species differing in pI, and all stimulated by ecdysone. Translations and hybrid‐arrested translations indicated that the poly(A)+ EIP mRNAs increase ˜10‐fold in abundance during the first 4 h of ecdysone treatment. By a differential screen of a cDNA library we have identified cDNA clones corresponding to all three EIPs. Two kinds of clones were isolated: one hybridizes to the EIP 40 mRNA(s); the second hybridizes to the mRNA(s) encoding all the EIPs 28 and 29. The EIP 28/29 and EIP 40 loci detected by these clones are each present at single sites on the polytene chromosomes and each is at or in the vicinity of an ecdysone‐regulated puff. |
| doi_str_mv | 10.1002/j.1460-2075.1984.tb01790.x |
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We have now shown, using two‐dimensional gels, that each of these EIPs consists of three species differing in pI, and all stimulated by ecdysone. Translations and hybrid‐arrested translations indicated that the poly(A)+ EIP mRNAs increase ˜10‐fold in abundance during the first 4 h of ecdysone treatment. By a differential screen of a cDNA library we have identified cDNA clones corresponding to all three EIPs. Two kinds of clones were isolated: one hybridizes to the EIP 40 mRNA(s); the second hybridizes to the mRNA(s) encoding all the EIPs 28 and 29. The EIP 28/29 and EIP 40 loci detected by these clones are each present at single sites on the polytene chromosomes and each is at or in the vicinity of an ecdysone‐regulated puff.</description><identifier>ISSN: 0261-4189</identifier><identifier>EISSN: 1460-2075</identifier><identifier>DOI: 10.1002/j.1460-2075.1984.tb01790.x</identifier><identifier>PMID: 16453496</identifier><identifier>CODEN: EMJODG</identifier><language>eng</language><publisher>London: Nature Publishing Group</publisher><subject>animal physiology ; arthropods ; Biological and medical sciences ; cDNA clones ; Cell physiology ; Drosophila ; Drosophilidae ; ecdysone ; entomology ; Fundamental and applied biological sciences. Psychology ; Hormonal regulation ; Kc cells ; Molecular and cellular biology ; steroid</subject><ispartof>The EMBO journal, 1984, Vol.3 (1), p.235-243</ispartof><rights>1984 European Molecular Biology Organization</rights><rights>1984 INIST-CNRS</rights><rights>IRL Press Limited, Oxford, England.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4110-6a5d6f318eef4ceedc199bbe96d4be9cbe8f1e38d99dd7742f3edb44edb119783</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC557326/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC557326/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,4025,27928,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9492302$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16453496$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Savakis, C</creatorcontrib><creatorcontrib>Koehler, M.M.D</creatorcontrib><creatorcontrib>Cherbas, P</creatorcontrib><title>cDNA clones for the ecdysone-inducible polypeptide (EIP) mRNAs of Drosophila Kc cells</title><title>The EMBO journal</title><addtitle>EMBO J</addtitle><description>The ecdysone‐inducible polypeptides (EIPs) 28, 29 and 40 were identified previously as polypeptides whose synthesis is stimulated early in the ecdysone response of Drosophila Kc cells. We have now shown, using two‐dimensional gels, that each of these EIPs consists of three species differing in pI, and all stimulated by ecdysone. Translations and hybrid‐arrested translations indicated that the poly(A)+ EIP mRNAs increase ˜10‐fold in abundance during the first 4 h of ecdysone treatment. By a differential screen of a cDNA library we have identified cDNA clones corresponding to all three EIPs. Two kinds of clones were isolated: one hybridizes to the EIP 40 mRNA(s); the second hybridizes to the mRNA(s) encoding all the EIPs 28 and 29. The EIP 28/29 and EIP 40 loci detected by these clones are each present at single sites on the polytene chromosomes and each is at or in the vicinity of an ecdysone‐regulated puff.</description><subject>animal physiology</subject><subject>arthropods</subject><subject>Biological and medical sciences</subject><subject>cDNA clones</subject><subject>Cell physiology</subject><subject>Drosophila</subject><subject>Drosophilidae</subject><subject>ecdysone</subject><subject>entomology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormonal regulation</subject><subject>Kc cells</subject><subject>Molecular and cellular biology</subject><subject>steroid</subject><issn>0261-4189</issn><issn>1460-2075</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><recordid>eNqVkV1v0zAUhiMEYt3gL4CFEB8XCT6x82EkLspWYDAGAnptOc7J6sqNs7iF9d_jkKjADRI3tuXzvK_P8RtFj4AmQGn6Yp0Az2mc0iJLQJQ82VYUCkGTm1vR7FC6Hc1omkPMoRRH0bH3a0ppVhZwNzqCnGeMi3wWLfXZ5Zxo61r0pHE92a6QoK73PtzEpq132lQWSefsvsNua2okzxbnn5-TzZfLuSeuIWe9865bGavIB000WuvvRXcaZT3en_aTaPlm8e30XXzx6e356fwi1hyAxrnK6rxhUCI2XCPWGoSoKhR5zcOqKywbQFbWQtR1UfC0YVhXnIcFQBQlO4lejb7drtoEObbbXlnZ9Waj-r10ysi_K61ZySv3XWZZwdI86J9O-t5d79Bv5cb4YQLVott5WTDGc8FoGsgn_ySBCcYhhwC-HEEdvsX32By6ASqH-ORaDhnJISM5xCen-ORNED_4c57f0imvADyeAOW1sk2vWm38gRNcpGO38xH7YSzu_6MDufj4-v2vc_B4OHo0ykl11Ydnll9TCsE_y7KSCvYTm7DCPw</recordid><startdate>1984</startdate><enddate>1984</enddate><creator>Savakis, C</creator><creator>Koehler, M.M.D</creator><creator>Cherbas, P</creator><general>Nature Publishing Group</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>1984</creationdate><title>cDNA clones for the ecdysone-inducible polypeptide (EIP) mRNAs of Drosophila Kc cells</title><author>Savakis, C ; Koehler, M.M.D ; Cherbas, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4110-6a5d6f318eef4ceedc199bbe96d4be9cbe8f1e38d99dd7742f3edb44edb119783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>animal physiology</topic><topic>arthropods</topic><topic>Biological and medical sciences</topic><topic>cDNA clones</topic><topic>Cell physiology</topic><topic>Drosophila</topic><topic>Drosophilidae</topic><topic>ecdysone</topic><topic>entomology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hormonal regulation</topic><topic>Kc cells</topic><topic>Molecular and cellular biology</topic><topic>steroid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Savakis, C</creatorcontrib><creatorcontrib>Koehler, M.M.D</creatorcontrib><creatorcontrib>Cherbas, P</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The EMBO journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Savakis, C</au><au>Koehler, M.M.D</au><au>Cherbas, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>cDNA clones for the ecdysone-inducible polypeptide (EIP) mRNAs of Drosophila Kc cells</atitle><jtitle>The EMBO journal</jtitle><addtitle>EMBO J</addtitle><date>1984</date><risdate>1984</risdate><volume>3</volume><issue>1</issue><spage>235</spage><epage>243</epage><pages>235-243</pages><issn>0261-4189</issn><eissn>1460-2075</eissn><coden>EMJODG</coden><abstract>The ecdysone‐inducible polypeptides (EIPs) 28, 29 and 40 were identified previously as polypeptides whose synthesis is stimulated early in the ecdysone response of Drosophila Kc cells. We have now shown, using two‐dimensional gels, that each of these EIPs consists of three species differing in pI, and all stimulated by ecdysone. Translations and hybrid‐arrested translations indicated that the poly(A)+ EIP mRNAs increase ˜10‐fold in abundance during the first 4 h of ecdysone treatment. By a differential screen of a cDNA library we have identified cDNA clones corresponding to all three EIPs. Two kinds of clones were isolated: one hybridizes to the EIP 40 mRNA(s); the second hybridizes to the mRNA(s) encoding all the EIPs 28 and 29. The EIP 28/29 and EIP 40 loci detected by these clones are each present at single sites on the polytene chromosomes and each is at or in the vicinity of an ecdysone‐regulated puff.</abstract><cop>London</cop><pub>Nature Publishing Group</pub><pmid>16453496</pmid><doi>10.1002/j.1460-2075.1984.tb01790.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | animal physiology arthropods Biological and medical sciences cDNA clones Cell physiology Drosophila Drosophilidae ecdysone entomology Fundamental and applied biological sciences. Psychology Hormonal regulation Kc cells Molecular and cellular biology steroid |
| title | cDNA clones for the ecdysone-inducible polypeptide (EIP) mRNAs of Drosophila Kc cells |
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