Expression of ADAMTS13 in Normal and Abnormal Placentae and Its Potential Role in Angiogenesis and Placenta Development
OBJECTIVE—ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin type 1 repeats, member 13) is primarily synthesized in liver. The biosynthesis of ADAMTS13 and its physiological role in placenta are not known. APPROACH AND RESULTS—We used real-time polymerase chain reaction, immunohistoch...
Gespeichert in:
| Veröffentlicht in: | Arteriosclerosis, thrombosis, and vascular biology thrombosis, and vascular biology, 2017-09, Vol.37 (9), p.1748-1756 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1756 |
|---|---|
| container_issue | 9 |
| container_start_page | 1748 |
| container_title | Arteriosclerosis, thrombosis, and vascular biology |
| container_volume | 37 |
| creator | Xiao, Juan Feng, Yun Li, Xueyin Li, Wei Fan, Lei Liu, Jing Zeng, Xue Chen, Kaiyue Chen, Xi Zhou, Xiaoshui Zheng, X Long Chen, Suhua |
| description | OBJECTIVE—ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin type 1 repeats, member 13) is primarily synthesized in liver. The biosynthesis of ADAMTS13 and its physiological role in placenta are not known.
APPROACH AND RESULTS—We used real-time polymerase chain reaction, immunohistochemistry, and Western blotting analyses, as well as proteolytic cleavage of FRETS (fluorescent resonance energy transfers)-VWF73, to determine ADAMTS13 expression in placenta and trophoblasts obtained from individuals with normal pregnancy and patients with severe preeclampsia. We also determined the role of ADAMTS13 in extravillous trophoblasts using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, wound scratch assay, transwell migration assay, tube formation assay, and tissue outgrowth assays. We showed that full-length and proteolytically active ADAMTS13 was expressed in normal human placenta, primarily in the trophoblasts and villous core fetal vessel endothelium during pregnancy. Placental expression of ADAMTS13 mRNA, protein, and proteolytic activity was at the highest levels during the first trimester and significantly reduced at the term of gestation. Additionally, significantly reduced levels of placental ADAMTS13 expression was detected under hypoxic conditions and in patients with preeclampsia. In addition, recombinant ADAMTS13 protease stimulated proliferation, migration, invasion, and network formation of trophoblastic cells in culture. Finally, knockdown of ADAMTS13 expression attenuated the ability of tube formation in trophoblast (HTR-8/SVNEO) cells and the extravillous trophoblast outgrowth in placental explants.
CONCLUSIONS—Our results demonstrate for the first time the expression of ADAMTS13 mRNA and protein in normal and abnormal placental tissues and its role in promoting angiogenesis and trophoblastic cell development. The findings support the potential role of the ADAMTS13–von Willebrand factor pathway in normal pregnancy and pathogenesis of preeclampsia. |
| doi_str_mv | 10.1161/ATVBAHA.117.309735 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5570641</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1924599413</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5155-ee118b191f8179d6d38286ed32b135719876dc594830c99bc885b3ae0439fc933</originalsourceid><addsrcrecordid>eNp9UU1v1DAUtBCIlsIf4IBy5JLij_jrghTa0lYqUMHC1XKSl12DE2_tbLf8e7zNtoILJ3vem5k30iD0muBjQgR5Vy9-fKgv6gzkMcNaMv4EHRJOq7ISTDzNfyx1yUVFD9CLlH5ijCtK8XN0QJXkhMvqEG3P7tYRUnJhLEJf1Kf1p8U3wgo3Fp9DHKwv7NgVdTPO4NrbFsbJwv34ckrFdZjywOXd1-Bhp6vHpQtLGCG5dE97EBWncAs-rIcMXqJnvfUJXu3fI_T949ni5KK8-nJ-eVJflW0OyEsAQlRDNOkVkboTHVNUCegYbQjjkmglRddyXSmGW62bVineMAu4YrpvNWNH6P3su940A3S7HNF6s45usPG3CdaZfzejW5lluDWcSywqkg3e7g1iuNlAmszgUgve2xHCJhmiacW1zsxMpTO1jSGlCP3jGYLNrjGzbywDaebGsujN3wEfJQ8VZYKYCdvgJ4jpl99sIZoVWD-t_uf8By0ao3k</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1924599413</pqid></control><display><type>article</type><title>Expression of ADAMTS13 in Normal and Abnormal Placentae and Its Potential Role in Angiogenesis and Placenta Development</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><source>Journals@Ovid Complete</source><creator>Xiao, Juan ; Feng, Yun ; Li, Xueyin ; Li, Wei ; Fan, Lei ; Liu, Jing ; Zeng, Xue ; Chen, Kaiyue ; Chen, Xi ; Zhou, Xiaoshui ; Zheng, X Long ; Chen, Suhua</creator><creatorcontrib>Xiao, Juan ; Feng, Yun ; Li, Xueyin ; Li, Wei ; Fan, Lei ; Liu, Jing ; Zeng, Xue ; Chen, Kaiyue ; Chen, Xi ; Zhou, Xiaoshui ; Zheng, X Long ; Chen, Suhua</creatorcontrib><description>OBJECTIVE—ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin type 1 repeats, member 13) is primarily synthesized in liver. The biosynthesis of ADAMTS13 and its physiological role in placenta are not known.
APPROACH AND RESULTS—We used real-time polymerase chain reaction, immunohistochemistry, and Western blotting analyses, as well as proteolytic cleavage of FRETS (fluorescent resonance energy transfers)-VWF73, to determine ADAMTS13 expression in placenta and trophoblasts obtained from individuals with normal pregnancy and patients with severe preeclampsia. We also determined the role of ADAMTS13 in extravillous trophoblasts using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, wound scratch assay, transwell migration assay, tube formation assay, and tissue outgrowth assays. We showed that full-length and proteolytically active ADAMTS13 was expressed in normal human placenta, primarily in the trophoblasts and villous core fetal vessel endothelium during pregnancy. Placental expression of ADAMTS13 mRNA, protein, and proteolytic activity was at the highest levels during the first trimester and significantly reduced at the term of gestation. Additionally, significantly reduced levels of placental ADAMTS13 expression was detected under hypoxic conditions and in patients with preeclampsia. In addition, recombinant ADAMTS13 protease stimulated proliferation, migration, invasion, and network formation of trophoblastic cells in culture. Finally, knockdown of ADAMTS13 expression attenuated the ability of tube formation in trophoblast (HTR-8/SVNEO) cells and the extravillous trophoblast outgrowth in placental explants.
CONCLUSIONS—Our results demonstrate for the first time the expression of ADAMTS13 mRNA and protein in normal and abnormal placental tissues and its role in promoting angiogenesis and trophoblastic cell development. The findings support the potential role of the ADAMTS13–von Willebrand factor pathway in normal pregnancy and pathogenesis of preeclampsia.</description><identifier>ISSN: 1079-5642</identifier><identifier>EISSN: 1524-4636</identifier><identifier>DOI: 10.1161/ATVBAHA.117.309735</identifier><identifier>PMID: 28751574</identifier><language>eng</language><publisher>United States: American Heart Association, Inc</publisher><subject>ADAMTS13 Protein - genetics ; ADAMTS13 Protein - metabolism ; Adult ; Animals ; Case-Control Studies ; Cell Movement ; Cell Proliferation ; CHO Cells ; Cricetulus ; Endothelial Cells - enzymology ; Female ; Gene Expression Regulation, Developmental ; Humans ; Neovascularization, Physiologic ; Placenta - blood supply ; Placenta - enzymology ; Placentation ; Pre-Eclampsia - enzymology ; Pre-Eclampsia - genetics ; Pre-Eclampsia - physiopathology ; Pregnancy ; Pregnancy Trimesters ; Proteolysis ; RNA Interference ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Signal Transduction ; Tissue Culture Techniques ; Transfection ; Trophoblasts - enzymology ; von Willebrand Factor - metabolism</subject><ispartof>Arteriosclerosis, thrombosis, and vascular biology, 2017-09, Vol.37 (9), p.1748-1756</ispartof><rights>2017 American Heart Association, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5155-ee118b191f8179d6d38286ed32b135719876dc594830c99bc885b3ae0439fc933</citedby><cites>FETCH-LOGICAL-c5155-ee118b191f8179d6d38286ed32b135719876dc594830c99bc885b3ae0439fc933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28751574$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xiao, Juan</creatorcontrib><creatorcontrib>Feng, Yun</creatorcontrib><creatorcontrib>Li, Xueyin</creatorcontrib><creatorcontrib>Li, Wei</creatorcontrib><creatorcontrib>Fan, Lei</creatorcontrib><creatorcontrib>Liu, Jing</creatorcontrib><creatorcontrib>Zeng, Xue</creatorcontrib><creatorcontrib>Chen, Kaiyue</creatorcontrib><creatorcontrib>Chen, Xi</creatorcontrib><creatorcontrib>Zhou, Xiaoshui</creatorcontrib><creatorcontrib>Zheng, X Long</creatorcontrib><creatorcontrib>Chen, Suhua</creatorcontrib><title>Expression of ADAMTS13 in Normal and Abnormal Placentae and Its Potential Role in Angiogenesis and Placenta Development</title><title>Arteriosclerosis, thrombosis, and vascular biology</title><addtitle>Arterioscler Thromb Vasc Biol</addtitle><description>OBJECTIVE—ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin type 1 repeats, member 13) is primarily synthesized in liver. The biosynthesis of ADAMTS13 and its physiological role in placenta are not known.
APPROACH AND RESULTS—We used real-time polymerase chain reaction, immunohistochemistry, and Western blotting analyses, as well as proteolytic cleavage of FRETS (fluorescent resonance energy transfers)-VWF73, to determine ADAMTS13 expression in placenta and trophoblasts obtained from individuals with normal pregnancy and patients with severe preeclampsia. We also determined the role of ADAMTS13 in extravillous trophoblasts using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, wound scratch assay, transwell migration assay, tube formation assay, and tissue outgrowth assays. We showed that full-length and proteolytically active ADAMTS13 was expressed in normal human placenta, primarily in the trophoblasts and villous core fetal vessel endothelium during pregnancy. Placental expression of ADAMTS13 mRNA, protein, and proteolytic activity was at the highest levels during the first trimester and significantly reduced at the term of gestation. Additionally, significantly reduced levels of placental ADAMTS13 expression was detected under hypoxic conditions and in patients with preeclampsia. In addition, recombinant ADAMTS13 protease stimulated proliferation, migration, invasion, and network formation of trophoblastic cells in culture. Finally, knockdown of ADAMTS13 expression attenuated the ability of tube formation in trophoblast (HTR-8/SVNEO) cells and the extravillous trophoblast outgrowth in placental explants.
CONCLUSIONS—Our results demonstrate for the first time the expression of ADAMTS13 mRNA and protein in normal and abnormal placental tissues and its role in promoting angiogenesis and trophoblastic cell development. The findings support the potential role of the ADAMTS13–von Willebrand factor pathway in normal pregnancy and pathogenesis of preeclampsia.</description><subject>ADAMTS13 Protein - genetics</subject><subject>ADAMTS13 Protein - metabolism</subject><subject>Adult</subject><subject>Animals</subject><subject>Case-Control Studies</subject><subject>Cell Movement</subject><subject>Cell Proliferation</subject><subject>CHO Cells</subject><subject>Cricetulus</subject><subject>Endothelial Cells - enzymology</subject><subject>Female</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Humans</subject><subject>Neovascularization, Physiologic</subject><subject>Placenta - blood supply</subject><subject>Placenta - enzymology</subject><subject>Placentation</subject><subject>Pre-Eclampsia - enzymology</subject><subject>Pre-Eclampsia - genetics</subject><subject>Pre-Eclampsia - physiopathology</subject><subject>Pregnancy</subject><subject>Pregnancy Trimesters</subject><subject>Proteolysis</subject><subject>RNA Interference</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Signal Transduction</subject><subject>Tissue Culture Techniques</subject><subject>Transfection</subject><subject>Trophoblasts - enzymology</subject><subject>von Willebrand Factor - metabolism</subject><issn>1079-5642</issn><issn>1524-4636</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU1v1DAUtBCIlsIf4IBy5JLij_jrghTa0lYqUMHC1XKSl12DE2_tbLf8e7zNtoILJ3vem5k30iD0muBjQgR5Vy9-fKgv6gzkMcNaMv4EHRJOq7ISTDzNfyx1yUVFD9CLlH5ijCtK8XN0QJXkhMvqEG3P7tYRUnJhLEJf1Kf1p8U3wgo3Fp9DHKwv7NgVdTPO4NrbFsbJwv34ckrFdZjywOXd1-Bhp6vHpQtLGCG5dE97EBWncAs-rIcMXqJnvfUJXu3fI_T949ni5KK8-nJ-eVJflW0OyEsAQlRDNOkVkboTHVNUCegYbQjjkmglRddyXSmGW62bVineMAu4YrpvNWNH6P3su940A3S7HNF6s45usPG3CdaZfzejW5lluDWcSywqkg3e7g1iuNlAmszgUgve2xHCJhmiacW1zsxMpTO1jSGlCP3jGYLNrjGzbywDaebGsujN3wEfJQ8VZYKYCdvgJ4jpl99sIZoVWD-t_uf8By0ao3k</recordid><startdate>201709</startdate><enddate>201709</enddate><creator>Xiao, Juan</creator><creator>Feng, Yun</creator><creator>Li, Xueyin</creator><creator>Li, Wei</creator><creator>Fan, Lei</creator><creator>Liu, Jing</creator><creator>Zeng, Xue</creator><creator>Chen, Kaiyue</creator><creator>Chen, Xi</creator><creator>Zhou, Xiaoshui</creator><creator>Zheng, X Long</creator><creator>Chen, Suhua</creator><general>American Heart Association, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201709</creationdate><title>Expression of ADAMTS13 in Normal and Abnormal Placentae and Its Potential Role in Angiogenesis and Placenta Development</title><author>Xiao, Juan ; Feng, Yun ; Li, Xueyin ; Li, Wei ; Fan, Lei ; Liu, Jing ; Zeng, Xue ; Chen, Kaiyue ; Chen, Xi ; Zhou, Xiaoshui ; Zheng, X Long ; Chen, Suhua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5155-ee118b191f8179d6d38286ed32b135719876dc594830c99bc885b3ae0439fc933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>ADAMTS13 Protein - genetics</topic><topic>ADAMTS13 Protein - metabolism</topic><topic>Adult</topic><topic>Animals</topic><topic>Case-Control Studies</topic><topic>Cell Movement</topic><topic>Cell Proliferation</topic><topic>CHO Cells</topic><topic>Cricetulus</topic><topic>Endothelial Cells - enzymology</topic><topic>Female</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Humans</topic><topic>Neovascularization, Physiologic</topic><topic>Placenta - blood supply</topic><topic>Placenta - enzymology</topic><topic>Placentation</topic><topic>Pre-Eclampsia - enzymology</topic><topic>Pre-Eclampsia - genetics</topic><topic>Pre-Eclampsia - physiopathology</topic><topic>Pregnancy</topic><topic>Pregnancy Trimesters</topic><topic>Proteolysis</topic><topic>RNA Interference</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Signal Transduction</topic><topic>Tissue Culture Techniques</topic><topic>Transfection</topic><topic>Trophoblasts - enzymology</topic><topic>von Willebrand Factor - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xiao, Juan</creatorcontrib><creatorcontrib>Feng, Yun</creatorcontrib><creatorcontrib>Li, Xueyin</creatorcontrib><creatorcontrib>Li, Wei</creatorcontrib><creatorcontrib>Fan, Lei</creatorcontrib><creatorcontrib>Liu, Jing</creatorcontrib><creatorcontrib>Zeng, Xue</creatorcontrib><creatorcontrib>Chen, Kaiyue</creatorcontrib><creatorcontrib>Chen, Xi</creatorcontrib><creatorcontrib>Zhou, Xiaoshui</creatorcontrib><creatorcontrib>Zheng, X Long</creatorcontrib><creatorcontrib>Chen, Suhua</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Arteriosclerosis, thrombosis, and vascular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xiao, Juan</au><au>Feng, Yun</au><au>Li, Xueyin</au><au>Li, Wei</au><au>Fan, Lei</au><au>Liu, Jing</au><au>Zeng, Xue</au><au>Chen, Kaiyue</au><au>Chen, Xi</au><au>Zhou, Xiaoshui</au><au>Zheng, X Long</au><au>Chen, Suhua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of ADAMTS13 in Normal and Abnormal Placentae and Its Potential Role in Angiogenesis and Placenta Development</atitle><jtitle>Arteriosclerosis, thrombosis, and vascular biology</jtitle><addtitle>Arterioscler Thromb Vasc Biol</addtitle><date>2017-09</date><risdate>2017</risdate><volume>37</volume><issue>9</issue><spage>1748</spage><epage>1756</epage><pages>1748-1756</pages><issn>1079-5642</issn><eissn>1524-4636</eissn><abstract>OBJECTIVE—ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin type 1 repeats, member 13) is primarily synthesized in liver. The biosynthesis of ADAMTS13 and its physiological role in placenta are not known.
APPROACH AND RESULTS—We used real-time polymerase chain reaction, immunohistochemistry, and Western blotting analyses, as well as proteolytic cleavage of FRETS (fluorescent resonance energy transfers)-VWF73, to determine ADAMTS13 expression in placenta and trophoblasts obtained from individuals with normal pregnancy and patients with severe preeclampsia. We also determined the role of ADAMTS13 in extravillous trophoblasts using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, wound scratch assay, transwell migration assay, tube formation assay, and tissue outgrowth assays. We showed that full-length and proteolytically active ADAMTS13 was expressed in normal human placenta, primarily in the trophoblasts and villous core fetal vessel endothelium during pregnancy. Placental expression of ADAMTS13 mRNA, protein, and proteolytic activity was at the highest levels during the first trimester and significantly reduced at the term of gestation. Additionally, significantly reduced levels of placental ADAMTS13 expression was detected under hypoxic conditions and in patients with preeclampsia. In addition, recombinant ADAMTS13 protease stimulated proliferation, migration, invasion, and network formation of trophoblastic cells in culture. Finally, knockdown of ADAMTS13 expression attenuated the ability of tube formation in trophoblast (HTR-8/SVNEO) cells and the extravillous trophoblast outgrowth in placental explants.
CONCLUSIONS—Our results demonstrate for the first time the expression of ADAMTS13 mRNA and protein in normal and abnormal placental tissues and its role in promoting angiogenesis and trophoblastic cell development. The findings support the potential role of the ADAMTS13–von Willebrand factor pathway in normal pregnancy and pathogenesis of preeclampsia.</abstract><cop>United States</cop><pub>American Heart Association, Inc</pub><pmid>28751574</pmid><doi>10.1161/ATVBAHA.117.309735</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1079-5642 |
| ispartof | Arteriosclerosis, thrombosis, and vascular biology, 2017-09, Vol.37 (9), p.1748-1756 |
| issn | 1079-5642 1524-4636 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5570641 |
| source | MEDLINE; Alma/SFX Local Collection; Journals@Ovid Complete |
| subjects | ADAMTS13 Protein - genetics ADAMTS13 Protein - metabolism Adult Animals Case-Control Studies Cell Movement Cell Proliferation CHO Cells Cricetulus Endothelial Cells - enzymology Female Gene Expression Regulation, Developmental Humans Neovascularization, Physiologic Placenta - blood supply Placenta - enzymology Placentation Pre-Eclampsia - enzymology Pre-Eclampsia - genetics Pre-Eclampsia - physiopathology Pregnancy Pregnancy Trimesters Proteolysis RNA Interference RNA, Messenger - genetics RNA, Messenger - metabolism Signal Transduction Tissue Culture Techniques Transfection Trophoblasts - enzymology von Willebrand Factor - metabolism |
| title | Expression of ADAMTS13 in Normal and Abnormal Placentae and Its Potential Role in Angiogenesis and Placenta Development |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T01%3A54%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20of%20ADAMTS13%20in%20Normal%20and%20Abnormal%20Placentae%20and%20Its%20Potential%20Role%20in%20Angiogenesis%20and%20Placenta%20Development&rft.jtitle=Arteriosclerosis,%20thrombosis,%20and%20vascular%20biology&rft.au=Xiao,%20Juan&rft.date=2017-09&rft.volume=37&rft.issue=9&rft.spage=1748&rft.epage=1756&rft.pages=1748-1756&rft.issn=1079-5642&rft.eissn=1524-4636&rft_id=info:doi/10.1161/ATVBAHA.117.309735&rft_dat=%3Cproquest_pubme%3E1924599413%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1924599413&rft_id=info:pmid/28751574&rfr_iscdi=true |