Three widely separated positions in the 16S RNA lie in or close to the ribosomal decoding region; a site‐directed cross‐linking study with mRNA analogues

Synthetic mRNA analogues were prepared by T7 transcription, each containing several thio‐uridine residues at selected positions. After binding to the ribosome in the presence of cognate tRNA, the thio‐U residues were activated by UV irradiation and the resulting sites of cross‐linking to 16S RNA ana...

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Veröffentlicht in:	The EMBO journal 1992-08, Vol.11 (8), p.3105-3116
Hauptverfasser:	Dontsova, O., Dokudovskaya, S., Kopylov, A., Bogdanov, A., Rinke‐Appel, J., Jünke, N., Brimacombe, R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Binding Sites Biological and medical sciences Cell structures and functions Cross-Linking Reagents Escherichia coli - genetics Escherichia coli - metabolism Fundamental and applied biological sciences. Psychology Models, Molecular Molecular and cellular biology Molecular Sequence Data Nucleic Acid Conformation Oligoribonucleotides - chemical synthesis Oligoribonucleotides - metabolism Ribonuclease H Ribosomal Proteins - metabolism Ribosome Ribosomes - metabolism RNA, Messenger - chemical synthesis RNA, Messenger - metabolism RNA, Messenger - radiation effects RNA, Ribosomal, 16S - metabolism RNA, Transfer - metabolism RNA-Directed DNA Polymerase - metabolism Structure-Activity Relationship Templates, Genetic Thiouridine Ultraviolet Rays
Online-Zugang:	Volltext
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