THE EFFECTS OF OXIDATIVE STRESS ON PHENOLIC COMPOSITION AND ANTIOXIDANT METABOLISM IN CALLUS CULTURE OF COMMON DAISY
Background: Exogenous pretreatment of oxidative stress on callus cultures of Bellis perennis L. (common daisy) induced catalase (CAT), superoxide dismutase (SOD), total phenolic, total flavonoid, total protein and selected commercial phenolic compounds production and accumulation. Materials and Meth...
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| Veröffentlicht in: | African journal of traditional, complementary, and alternative medicines complementary, and alternative medicines, 2016-01, Vol.13 (4), p.34-41 |
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| creator | Karakas, Fatma Pehlivan Cingoz, Gunce Sahin Turker, Arzu Ucar |
| description | Background: Exogenous pretreatment of oxidative stress on callus
cultures of Bellis perennis L. (common daisy) induced catalase (CAT),
superoxide dismutase (SOD), total phenolic, total flavonoid, total
protein and selected commercial phenolic compounds production and
accumulation. Materials and Methods: The callus culture obtained from
B. perennis pedicel explants was incubated on Murashige and Skoog
medium (MS) containing 10 mM H2O2 or 0 mM H2O2 (for control group) for
10 hours. Twenty phenolic compounds (apigenin, caffeic acid, p-coumaric
acid, gallic acid, genistein, kaempferol, luteolin, myricetin,
procyanidin-C1, quercetin, rutin hydrate, vanillic acid, ferulic acid,
salicylic acid, sinapic acid, chlorogenic acid, hesperedin, naringenin,
rosmarinic acid and isorhamnetin) were detected by LC-ESI-MS/MS
analysis in methanolic extracts of 10 mM H2O2 and control treatments.
Results: A predominant phenolic compound was chlorogenic acid followed
by rutin hydrate, caffeic acid, luteoline, isorhamnetin, quercetin,
myricetin, apigenin, p-coumaric acid and kaempferol. No gallic acid,
genistein, procyanidin-C1, vanillic acid, sinapic acid, hesperidin and
naringenin were detected in H2O2 treatment and control groups of B.
perennis. The total phenolic contents estimated were in the order of
H2O2 treatment (285.36 μg/g dw) and control (220.79 μg/g dw)
groups. The biosynthesis and accumulation of kaempferol, myricetin,
quercetin and isorhamnetin were only determined in H2O2 treatment
callus materials. The H2O2 pretreatment clearly showed in a raise in
enzymatic and non-enzymatic antioxidant activities. Finally, a
significant positive correlation between phenolic accumulation and
comprehensive activities of CAT, SOD, total phenolic, total flavonoid
and proline was accessible. Conclusion: The present results suggest
that using H2O2 as an elicitor or a stimulant plays a significant
enhancement role in special phenolic molecules biosynthesis and
activation of antioxidant metabolism on callus cultures of B. perennis. |
| doi_str_mv | 10.21010/ajtcam.v13i4.6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5566151</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1933938569</sourcerecordid><originalsourceid>FETCH-LOGICAL-b391t-af88de19be5d5977385d44be236d15a6a9c8d0c32473c03bfbbb9221bff540783</originalsourceid><addsrcrecordid>eNpVkc9v0zAcxS0EYmVw5oZ85JLOP2LHuSCF1KWR0mRaUgQny3YcyJQ2W5JO4r_HW7cKJFu2nj_v-Ss9AD5itCQYYXSlb2er98sHTLtwyV-BBWGIBQiF4WuwQFjEAUeYX4B303SLEBWYRG_BBRGCkQiLBZjrjYRyvZZpXcFyDcsf2Sqps-8SVvWNrLxWwOuNLMo8S2Fabq_LKqszLybFym9_fTQUNdzKOvnqqWoLswKmSZ7vKpju8np3Ix-DvXfrbaskq36-B29a3U_uw_N5CXZrWaebIC-_Zd4aGBrjOdCtEI3DsXGsYXEUUcGaMDSOUN5gprmOrWiQpSSMqEXUtMaYmBBs2paFKBL0Enw55d4dzd411h3mUffqbuz2evyjBt2p_18O3W_1a3hQjHGOGfYBn58DxuH-6KZZ7bvJur7XBzccJ4VjSmM_Fo89enVC7ThM0-ja8zcYqaeu1Kkr9dSV4t7x6d_pzvxLOR5YngDTDX13cGfCjp1WL-Js_cIcCUb_AsVSmnQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1933938569</pqid></control><display><type>article</type><title>THE EFFECTS OF OXIDATIVE STRESS ON PHENOLIC COMPOSITION AND ANTIOXIDANT METABOLISM IN CALLUS CULTURE OF COMMON DAISY</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Bioline International</source><source>PubMed Central</source><creator>Karakas, Fatma Pehlivan ; Cingoz, Gunce Sahin ; Turker, Arzu Ucar</creator><creatorcontrib>Karakas, Fatma Pehlivan ; Cingoz, Gunce Sahin ; Turker, Arzu Ucar</creatorcontrib><description>Background: Exogenous pretreatment of oxidative stress on callus
cultures of Bellis perennis L. (common daisy) induced catalase (CAT),
superoxide dismutase (SOD), total phenolic, total flavonoid, total
protein and selected commercial phenolic compounds production and
accumulation. Materials and Methods: The callus culture obtained from
B. perennis pedicel explants was incubated on Murashige and Skoog
medium (MS) containing 10 mM H2O2 or 0 mM H2O2 (for control group) for
10 hours. Twenty phenolic compounds (apigenin, caffeic acid, p-coumaric
acid, gallic acid, genistein, kaempferol, luteolin, myricetin,
procyanidin-C1, quercetin, rutin hydrate, vanillic acid, ferulic acid,
salicylic acid, sinapic acid, chlorogenic acid, hesperedin, naringenin,
rosmarinic acid and isorhamnetin) were detected by LC-ESI-MS/MS
analysis in methanolic extracts of 10 mM H2O2 and control treatments.
Results: A predominant phenolic compound was chlorogenic acid followed
by rutin hydrate, caffeic acid, luteoline, isorhamnetin, quercetin,
myricetin, apigenin, p-coumaric acid and kaempferol. No gallic acid,
genistein, procyanidin-C1, vanillic acid, sinapic acid, hesperidin and
naringenin were detected in H2O2 treatment and control groups of B.
perennis. The total phenolic contents estimated were in the order of
H2O2 treatment (285.36 μg/g dw) and control (220.79 μg/g dw)
groups. The biosynthesis and accumulation of kaempferol, myricetin,
quercetin and isorhamnetin were only determined in H2O2 treatment
callus materials. The H2O2 pretreatment clearly showed in a raise in
enzymatic and non-enzymatic antioxidant activities. Finally, a
significant positive correlation between phenolic accumulation and
comprehensive activities of CAT, SOD, total phenolic, total flavonoid
and proline was accessible. Conclusion: The present results suggest
that using H2O2 as an elicitor or a stimulant plays a significant
enhancement role in special phenolic molecules biosynthesis and
activation of antioxidant metabolism on callus cultures of B. perennis.</description><identifier>ISSN: 0189-6016</identifier><identifier>EISSN: 2505-0044</identifier><identifier>DOI: 10.21010/ajtcam.v13i4.6</identifier><identifier>PMID: 28852718</identifier><language>eng</language><publisher>Nigeria: African Ethnomedicines Network</publisher><subject>antioxidant activity ; Antioxidants - chemistry ; Antioxidants - metabolism ; Asteraceae - chemistry ; Asteraceae - drug effects ; Asteraceae - growth & development ; Asteraceae - metabolism ; Bellis perennis ; callus culture ; CAT ; Cells, Cultured ; hydrogen peroxide ; Hydrogen Peroxide - pharmacology ; Oxidative Stress - drug effects ; pedicel explant ; Phenols - chemistry ; Plant Extracts - chemistry ; Plant Extracts - metabolism ; SOD</subject><ispartof>African journal of traditional, complementary, and alternative medicines, 2016-01, Vol.13 (4), p.34-41</ispartof><rights>Copyright 2016 - African Journal of Traditional, Complementary and Alternative Medicines</rights><rights>Copyright: © 2016 Afr. J. Traditional Complementary and Alternative Medicines 2016</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b391t-af88de19be5d5977385d44be236d15a6a9c8d0c32473c03bfbbb9221bff540783</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5566151/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5566151/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,886,27929,27930,53796,53798,79431</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28852718$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Karakas, Fatma Pehlivan</creatorcontrib><creatorcontrib>Cingoz, Gunce Sahin</creatorcontrib><creatorcontrib>Turker, Arzu Ucar</creatorcontrib><title>THE EFFECTS OF OXIDATIVE STRESS ON PHENOLIC COMPOSITION AND ANTIOXIDANT METABOLISM IN CALLUS CULTURE OF COMMON DAISY</title><title>African journal of traditional, complementary, and alternative medicines</title><addtitle>Afr J Tradit Complement Altern Med</addtitle><description>Background: Exogenous pretreatment of oxidative stress on callus
cultures of Bellis perennis L. (common daisy) induced catalase (CAT),
superoxide dismutase (SOD), total phenolic, total flavonoid, total
protein and selected commercial phenolic compounds production and
accumulation. Materials and Methods: The callus culture obtained from
B. perennis pedicel explants was incubated on Murashige and Skoog
medium (MS) containing 10 mM H2O2 or 0 mM H2O2 (for control group) for
10 hours. Twenty phenolic compounds (apigenin, caffeic acid, p-coumaric
acid, gallic acid, genistein, kaempferol, luteolin, myricetin,
procyanidin-C1, quercetin, rutin hydrate, vanillic acid, ferulic acid,
salicylic acid, sinapic acid, chlorogenic acid, hesperedin, naringenin,
rosmarinic acid and isorhamnetin) were detected by LC-ESI-MS/MS
analysis in methanolic extracts of 10 mM H2O2 and control treatments.
Results: A predominant phenolic compound was chlorogenic acid followed
by rutin hydrate, caffeic acid, luteoline, isorhamnetin, quercetin,
myricetin, apigenin, p-coumaric acid and kaempferol. No gallic acid,
genistein, procyanidin-C1, vanillic acid, sinapic acid, hesperidin and
naringenin were detected in H2O2 treatment and control groups of B.
perennis. The total phenolic contents estimated were in the order of
H2O2 treatment (285.36 μg/g dw) and control (220.79 μg/g dw)
groups. The biosynthesis and accumulation of kaempferol, myricetin,
quercetin and isorhamnetin were only determined in H2O2 treatment
callus materials. The H2O2 pretreatment clearly showed in a raise in
enzymatic and non-enzymatic antioxidant activities. Finally, a
significant positive correlation between phenolic accumulation and
comprehensive activities of CAT, SOD, total phenolic, total flavonoid
and proline was accessible. Conclusion: The present results suggest
that using H2O2 as an elicitor or a stimulant plays a significant
enhancement role in special phenolic molecules biosynthesis and
activation of antioxidant metabolism on callus cultures of B. perennis.</description><subject>antioxidant activity</subject><subject>Antioxidants - chemistry</subject><subject>Antioxidants - metabolism</subject><subject>Asteraceae - chemistry</subject><subject>Asteraceae - drug effects</subject><subject>Asteraceae - growth & development</subject><subject>Asteraceae - metabolism</subject><subject>Bellis perennis</subject><subject>callus culture</subject><subject>CAT</subject><subject>Cells, Cultured</subject><subject>hydrogen peroxide</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>Oxidative Stress - drug effects</subject><subject>pedicel explant</subject><subject>Phenols - chemistry</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - metabolism</subject><subject>SOD</subject><issn>0189-6016</issn><issn>2505-0044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>RBI</sourceid><sourceid>EIF</sourceid><recordid>eNpVkc9v0zAcxS0EYmVw5oZ85JLOP2LHuSCF1KWR0mRaUgQny3YcyJQ2W5JO4r_HW7cKJFu2nj_v-Ss9AD5itCQYYXSlb2er98sHTLtwyV-BBWGIBQiF4WuwQFjEAUeYX4B303SLEBWYRG_BBRGCkQiLBZjrjYRyvZZpXcFyDcsf2Sqps-8SVvWNrLxWwOuNLMo8S2Fabq_LKqszLybFym9_fTQUNdzKOvnqqWoLswKmSZ7vKpju8np3Ix-DvXfrbaskq36-B29a3U_uw_N5CXZrWaebIC-_Zd4aGBrjOdCtEI3DsXGsYXEUUcGaMDSOUN5gprmOrWiQpSSMqEXUtMaYmBBs2paFKBL0Enw55d4dzd411h3mUffqbuz2evyjBt2p_18O3W_1a3hQjHGOGfYBn58DxuH-6KZZ7bvJur7XBzccJ4VjSmM_Fo89enVC7ThM0-ja8zcYqaeu1Kkr9dSV4t7x6d_pzvxLOR5YngDTDX13cGfCjp1WL-Js_cIcCUb_AsVSmnQ</recordid><startdate>20160101</startdate><enddate>20160101</enddate><creator>Karakas, Fatma Pehlivan</creator><creator>Cingoz, Gunce Sahin</creator><creator>Turker, Arzu Ucar</creator><general>African Ethnomedicines Network</general><general>African Traditional Herbal Medicine Supporters Initiative (ATHMSI)</general><scope>RBI</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20160101</creationdate><title>THE EFFECTS OF OXIDATIVE STRESS ON PHENOLIC COMPOSITION AND ANTIOXIDANT METABOLISM IN CALLUS CULTURE OF COMMON DAISY</title><author>Karakas, Fatma Pehlivan ; Cingoz, Gunce Sahin ; Turker, Arzu Ucar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b391t-af88de19be5d5977385d44be236d15a6a9c8d0c32473c03bfbbb9221bff540783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>antioxidant activity</topic><topic>Antioxidants - chemistry</topic><topic>Antioxidants - metabolism</topic><topic>Asteraceae - chemistry</topic><topic>Asteraceae - drug effects</topic><topic>Asteraceae - growth & development</topic><topic>Asteraceae - metabolism</topic><topic>Bellis perennis</topic><topic>callus culture</topic><topic>CAT</topic><topic>Cells, Cultured</topic><topic>hydrogen peroxide</topic><topic>Hydrogen Peroxide - pharmacology</topic><topic>Oxidative Stress - drug effects</topic><topic>pedicel explant</topic><topic>Phenols - chemistry</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - metabolism</topic><topic>SOD</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Karakas, Fatma Pehlivan</creatorcontrib><creatorcontrib>Cingoz, Gunce Sahin</creatorcontrib><creatorcontrib>Turker, Arzu Ucar</creatorcontrib><collection>Bioline International</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>African journal of traditional, complementary, and alternative medicines</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Karakas, Fatma Pehlivan</au><au>Cingoz, Gunce Sahin</au><au>Turker, Arzu Ucar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>THE EFFECTS OF OXIDATIVE STRESS ON PHENOLIC COMPOSITION AND ANTIOXIDANT METABOLISM IN CALLUS CULTURE OF COMMON DAISY</atitle><jtitle>African journal of traditional, complementary, and alternative medicines</jtitle><addtitle>Afr J Tradit Complement Altern Med</addtitle><date>2016-01-01</date><risdate>2016</risdate><volume>13</volume><issue>4</issue><spage>34</spage><epage>41</epage><pages>34-41</pages><issn>0189-6016</issn><eissn>2505-0044</eissn><abstract>Background: Exogenous pretreatment of oxidative stress on callus
cultures of Bellis perennis L. (common daisy) induced catalase (CAT),
superoxide dismutase (SOD), total phenolic, total flavonoid, total
protein and selected commercial phenolic compounds production and
accumulation. Materials and Methods: The callus culture obtained from
B. perennis pedicel explants was incubated on Murashige and Skoog
medium (MS) containing 10 mM H2O2 or 0 mM H2O2 (for control group) for
10 hours. Twenty phenolic compounds (apigenin, caffeic acid, p-coumaric
acid, gallic acid, genistein, kaempferol, luteolin, myricetin,
procyanidin-C1, quercetin, rutin hydrate, vanillic acid, ferulic acid,
salicylic acid, sinapic acid, chlorogenic acid, hesperedin, naringenin,
rosmarinic acid and isorhamnetin) were detected by LC-ESI-MS/MS
analysis in methanolic extracts of 10 mM H2O2 and control treatments.
Results: A predominant phenolic compound was chlorogenic acid followed
by rutin hydrate, caffeic acid, luteoline, isorhamnetin, quercetin,
myricetin, apigenin, p-coumaric acid and kaempferol. No gallic acid,
genistein, procyanidin-C1, vanillic acid, sinapic acid, hesperidin and
naringenin were detected in H2O2 treatment and control groups of B.
perennis. The total phenolic contents estimated were in the order of
H2O2 treatment (285.36 μg/g dw) and control (220.79 μg/g dw)
groups. The biosynthesis and accumulation of kaempferol, myricetin,
quercetin and isorhamnetin were only determined in H2O2 treatment
callus materials. The H2O2 pretreatment clearly showed in a raise in
enzymatic and non-enzymatic antioxidant activities. Finally, a
significant positive correlation between phenolic accumulation and
comprehensive activities of CAT, SOD, total phenolic, total flavonoid
and proline was accessible. Conclusion: The present results suggest
that using H2O2 as an elicitor or a stimulant plays a significant
enhancement role in special phenolic molecules biosynthesis and
activation of antioxidant metabolism on callus cultures of B. perennis.</abstract><cop>Nigeria</cop><pub>African Ethnomedicines Network</pub><pmid>28852718</pmid><doi>10.21010/ajtcam.v13i4.6</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0189-6016 |
| ispartof | African journal of traditional, complementary, and alternative medicines, 2016-01, Vol.13 (4), p.34-41 |
| issn | 0189-6016 2505-0044 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5566151 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Bioline International; PubMed Central |
| subjects | antioxidant activity Antioxidants - chemistry Antioxidants - metabolism Asteraceae - chemistry Asteraceae - drug effects Asteraceae - growth & development Asteraceae - metabolism Bellis perennis callus culture CAT Cells, Cultured hydrogen peroxide Hydrogen Peroxide - pharmacology Oxidative Stress - drug effects pedicel explant Phenols - chemistry Plant Extracts - chemistry Plant Extracts - metabolism SOD |
| title | THE EFFECTS OF OXIDATIVE STRESS ON PHENOLIC COMPOSITION AND ANTIOXIDANT METABOLISM IN CALLUS CULTURE OF COMMON DAISY |
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