An ultra-sensitive biophysical risk assessment of light effect on skin cells
The aim of this study was to analyze photo-dynamic and photo-pathology changes of different color light radiations on human adult skin cells. We used a real-time biophysical and biomechanics monitoring system for light-induced cellular changes in an in vitro model to find mechanisms of the initial a...
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| Veröffentlicht in: | Oncotarget 2017-07, Vol.8 (29), p.47861-47875 |
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| creator | Bennet, Devasier Viswanath, Buddolla Kim, Sanghyo An, Jeong Ho |
| description | The aim of this study was to analyze photo-dynamic and photo-pathology changes of different color light radiations on human adult skin cells. We used a real-time biophysical and biomechanics monitoring system for light-induced cellular changes in an in vitro model to find mechanisms of the initial and continuous degenerative process. Cells were exposed to intermittent, mild and intense (1-180 min) light with On/Off cycles, using blue, green, red and white light. Cellular ultra-structural changes, damages, and ECM impair function were evaluated by up/down-regulation of biophysical, biomechanical and biochemical properties. All cells exposed to different color light radiation showed significant changes in a time-dependent manner. Particularly, cell growth, stiffness, roughness, cytoskeletal integrity and ECM proteins of the human dermal fibroblasts-adult (HDF-a) cells showed highest alteration, followed by human epidermal keratinocytes-adult (HEK-a) cells and human epidermal melanocytes-adult (HEM-a) cells. Such changes might impede the normal cellular functions. Overall, the obtained results identify a new insight that may contribute to premature aging, and causes it to look aged in younger people. Moreover, these results advance our understanding of the different color light-induced degenerative process and help the development of new therapeutic strategies. |
| doi_str_mv | 10.18632/oncotarget.18136 |
| format | Article |
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We used a real-time biophysical and biomechanics monitoring system for light-induced cellular changes in an in vitro model to find mechanisms of the initial and continuous degenerative process. Cells were exposed to intermittent, mild and intense (1-180 min) light with On/Off cycles, using blue, green, red and white light. Cellular ultra-structural changes, damages, and ECM impair function were evaluated by up/down-regulation of biophysical, biomechanical and biochemical properties. All cells exposed to different color light radiation showed significant changes in a time-dependent manner. Particularly, cell growth, stiffness, roughness, cytoskeletal integrity and ECM proteins of the human dermal fibroblasts-adult (HDF-a) cells showed highest alteration, followed by human epidermal keratinocytes-adult (HEK-a) cells and human epidermal melanocytes-adult (HEM-a) cells. Such changes might impede the normal cellular functions. Overall, the obtained results identify a new insight that may contribute to premature aging, and causes it to look aged in younger people. Moreover, these results advance our understanding of the different color light-induced degenerative process and help the development of new therapeutic strategies.</description><identifier>ISSN: 1949-2553</identifier><identifier>EISSN: 1949-2553</identifier><identifier>DOI: 10.18632/oncotarget.18136</identifier><identifier>PMID: 28599308</identifier><language>eng</language><publisher>United States: Impact Journals LLC</publisher><subject>Biophysics - methods ; Cell Line ; Electric Impedance ; Extracellular Matrix - metabolism ; Extracellular Matrix - radiation effects ; Flow Cytometry ; Humans ; Light - adverse effects ; Microscopy, Atomic Force ; Research Paper ; Risk Assessment ; Skin - cytology ; Skin - radiation effects ; Skin Physiological Phenomena - radiation effects</subject><ispartof>Oncotarget, 2017-07, Vol.8 (29), p.47861-47875</ispartof><rights>Copyright: © 2017 Bennet et al. 2017</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-dfe7c99c62d4e4654b240088c064a5ad5c6d1ab2ec62da57dc70048be47d61f73</citedby><cites>FETCH-LOGICAL-c356t-dfe7c99c62d4e4654b240088c064a5ad5c6d1ab2ec62da57dc70048be47d61f73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5564611/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5564611/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27915,27916,53782,53784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28599308$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bennet, Devasier</creatorcontrib><creatorcontrib>Viswanath, Buddolla</creatorcontrib><creatorcontrib>Kim, Sanghyo</creatorcontrib><creatorcontrib>An, Jeong Ho</creatorcontrib><title>An ultra-sensitive biophysical risk assessment of light effect on skin cells</title><title>Oncotarget</title><addtitle>Oncotarget</addtitle><description>The aim of this study was to analyze photo-dynamic and photo-pathology changes of different color light radiations on human adult skin cells. 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We used a real-time biophysical and biomechanics monitoring system for light-induced cellular changes in an in vitro model to find mechanisms of the initial and continuous degenerative process. Cells were exposed to intermittent, mild and intense (1-180 min) light with On/Off cycles, using blue, green, red and white light. Cellular ultra-structural changes, damages, and ECM impair function were evaluated by up/down-regulation of biophysical, biomechanical and biochemical properties. All cells exposed to different color light radiation showed significant changes in a time-dependent manner. Particularly, cell growth, stiffness, roughness, cytoskeletal integrity and ECM proteins of the human dermal fibroblasts-adult (HDF-a) cells showed highest alteration, followed by human epidermal keratinocytes-adult (HEK-a) cells and human epidermal melanocytes-adult (HEM-a) cells. Such changes might impede the normal cellular functions. 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| source | MEDLINE; PubMed Central; Free E- Journals; EZB Electronic Journals Library; PubMed Central Open Access |
| subjects | Biophysics - methods Cell Line Electric Impedance Extracellular Matrix - metabolism Extracellular Matrix - radiation effects Flow Cytometry Humans Light - adverse effects Microscopy, Atomic Force Research Paper Risk Assessment Skin - cytology Skin - radiation effects Skin Physiological Phenomena - radiation effects |
| title | An ultra-sensitive biophysical risk assessment of light effect on skin cells |
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