Reconstituted mammalian U4/U6 snRNP complements splicing: a mutational analysis

We have developed an in vitro complementation assay to analyse the functions of U6 small nuclear RNA (snRNA) in splicing and in the assembly of small nuclear ribonucleoproteins (snRNPs) and spliceosomes. U6‐specific, biotinylated 2′‐OMe RNA oligonucleotides were used to deplete nuclear extract of th...

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Veröffentlicht in:	The EMBO journal 1992-01, Vol.11 (1), p.345-359
Hauptverfasser:	Wolff, T., Bindereif, A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - metabolism Base Sequence Biological and medical sciences Cell Nucleus - metabolism Chromatography, Affinity DNA Mutational Analysis Fundamental and applied biological sciences. Psychology Genetic Complementation Test HeLa Cells Humans Models, Molecular Molecular and cellular biology Molecular genetics Molecular Sequence Data Nucleic Acid Conformation RNA Splicing RNA, Small Nuclear - genetics RNA, Small Nuclear - isolation & purification RNA, Small Nuclear - metabolism Streptavidin Subcellular Fractions Transcription. Transcription factor. Splicing. Rna processing
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