Neurotrophic effects of 7,8-dihydroxycoumarin in primary cultured rat cortical neurons
Objective Neuronal loss in the central nervous system is central to the occurrence of neurodegenerative diseases. Pharmaceutical companies have devoted much effort to developing new drugs against such diseases, since there are currently no effective drugs for neurodegenerative disease treatment. Pro...
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| Veröffentlicht in: | Neuroscience bulletin 2012-10, Vol.28 (5), p.493-498 |
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| description | Objective Neuronal loss in the central nervous system is central to the occurrence of neurodegenerative diseases. Pharmaceutical companies have devoted much effort to developing new drugs against such diseases, since there are currently no effective drugs for neurodegenerative disease treatment. Promoting the capacity for nerve regeneration is an ideal treatment target. The present study aimed to investigate the neurotrophic effects of 7,8-dihydroxycoumarin （DHC） or daphnetin in primary cultured rat cortical neurons. Methods Cortical neurons were identified by microtubule-associated protein 2 （MAP2） immunostaining. Morphological observation was used to measure the average length of neurite outgrowth. MTT and lactate dehydrogenase assays were used to assess neuronal survival. The mRNA expression of MAP2 and brain-derived neurotrophic factor （BDNF） was measured by RT-PCR. Results MAP2 immunostaining showed that most of the cultured cells were neurons. Compared with the vehicle control group, DHC promoted neurite outgrowth and prolonged neuronal survival time at concentrations ranging from 2 to 8 μmol/L. Expression of both BDNF mRNA and MAP2 mRNAwas increased in the groups treated with 2, 4 and 8 μmol/L DHC. Conclusion DHC significantly increases neurite outgrowth and promotes neuronal survival in primary cultured rat cortical neurons. The neurotrophic effects of DHC are probably associated with increased BDNF expression. |
| doi_str_mv | 10.1007/s12264-012-1233-7 |
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| fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5560254</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>43876106</cqvip_id><sourcerecordid>22961470</sourcerecordid><originalsourceid>FETCH-LOGICAL-c468t-c18592b7ebd2bbc66b2c88187728e284850b9ad797df0ff7abfc9bcf7396019a3</originalsourceid><addsrcrecordid>eNp9kF1LwzAUhoMoTqc_wBup90aTtM3HjSDDLxh6o96GJE3Wjq2ZSSvu35vROfRGCOSQc94nhweAM4yuMELsOmJCaAERJhCTPIdsDxxhIUrICeb7qaYsPSLKRuA4xjlCFLG8OAQjQgTFBUNH4P3Z9sF3wa_qxmTWOWu6mHmXsUsOq6ZeV8F_rY3vlyo0bZbOKjSpXmemX3R9sFUWVJcZH7rGqEXWbnBtPAEHTi2iPd3eY_B2f_c6eYTTl4enye0UmoLyDhrMS0E0s7oiWhtKNTGcY84Y4ZbwgpdIC1UxwSqHnGNKOyO0cSwXFGGh8jG4GbirXi9tZWzbBbWQ2x2lV43822mbWs78pyxLikhZJAAeACb4GIN1uyxGciNZDpJlkiw3kiVLmfPfn-4SP1bTABkGYmq1Mxvk3PehTSL-pV5sN6l9O_tIuR24yDmjGNH8GxvjldE</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Neurotrophic effects of 7,8-dihydroxycoumarin in primary cultured rat cortical neurons</title><source>MEDLINE</source><source>SpringerLink Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Yan, Li ; Zhou, Xiaowen ; Zhou, Xing ; Zhang, Zheng ; Luo, Huan-Min</creator><creatorcontrib>Yan, Li ; Zhou, Xiaowen ; Zhou, Xing ; Zhang, Zheng ; Luo, Huan-Min</creatorcontrib><description>Objective Neuronal loss in the central nervous system is central to the occurrence of neurodegenerative diseases. Pharmaceutical companies have devoted much effort to developing new drugs against such diseases, since there are currently no effective drugs for neurodegenerative disease treatment. Promoting the capacity for nerve regeneration is an ideal treatment target. The present study aimed to investigate the neurotrophic effects of 7,8-dihydroxycoumarin （DHC） or daphnetin in primary cultured rat cortical neurons. Methods Cortical neurons were identified by microtubule-associated protein 2 （MAP2） immunostaining. Morphological observation was used to measure the average length of neurite outgrowth. MTT and lactate dehydrogenase assays were used to assess neuronal survival. The mRNA expression of MAP2 and brain-derived neurotrophic factor （BDNF） was measured by RT-PCR. Results MAP2 immunostaining showed that most of the cultured cells were neurons. Compared with the vehicle control group, DHC promoted neurite outgrowth and prolonged neuronal survival time at concentrations ranging from 2 to 8 μmol/L. Expression of both BDNF mRNA and MAP2 mRNAwas increased in the groups treated with 2, 4 and 8 μmol/L DHC. Conclusion DHC significantly increases neurite outgrowth and promotes neuronal survival in primary cultured rat cortical neurons. The neurotrophic effects of DHC are probably associated with increased BDNF expression.</description><identifier>ISSN: 1673-7067</identifier><identifier>EISSN: 1995-8218</identifier><identifier>DOI: 10.1007/s12264-012-1233-7</identifier><identifier>PMID: 22961470</identifier><language>eng</language><publisher>Heidelberg: Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences</publisher><subject>Anatomy ; Anesthesiology ; Animals ; Animals, Newborn ; Biomedical and Life Sciences ; Biomedicine ; Cell Survival - drug effects ; Cell Survival - physiology ; Cerebral Cortex - cytology ; Cerebral Cortex - drug effects ; Cerebral Cortex - physiology ; Dose-Response Relationship, Drug ; Human Physiology ; mRNA表达 ; Nerve Growth Factors - chemistry ; Nerve Growth Factors - pharmacology ; Nerve Regeneration - drug effects ; Nerve Regeneration - physiology ; Neurology ; Neurons - drug effects ; Neurons - physiology ; Neurosciences ; Original ; Original Article ; Pain Medicine ; Primary Cell Culture ; Rats ; Rats, Sprague-Dawley ; Umbelliferones - chemistry ; Umbelliferones - pharmacology ; 原代培养 ; 大鼠 ; 皮层神经元 ; 神经营养作用 ; 神经退行性疾病 ; 羟基香豆素 ; 脑源性神经营养因子</subject><ispartof>Neuroscience bulletin, 2012-10, Vol.28 (5), p.493-498</ispartof><rights>Shanghai Institutes for Biological Sciences, CAS and Springer-Verlag Berlin Heidelberg 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c468t-c18592b7ebd2bbc66b2c88187728e284850b9ad797df0ff7abfc9bcf7396019a3</citedby><cites>FETCH-LOGICAL-c468t-c18592b7ebd2bbc66b2c88187728e284850b9ad797df0ff7abfc9bcf7396019a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/91222B/91222B.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5560254/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5560254/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,41488,42557,51319,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22961470$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yan, Li</creatorcontrib><creatorcontrib>Zhou, Xiaowen</creatorcontrib><creatorcontrib>Zhou, Xing</creatorcontrib><creatorcontrib>Zhang, Zheng</creatorcontrib><creatorcontrib>Luo, Huan-Min</creatorcontrib><title>Neurotrophic effects of 7,8-dihydroxycoumarin in primary cultured rat cortical neurons</title><title>Neuroscience bulletin</title><addtitle>Neurosci. Bull</addtitle><addtitle>Neuroscience Bulletin</addtitle><description>Objective Neuronal loss in the central nervous system is central to the occurrence of neurodegenerative diseases. Pharmaceutical companies have devoted much effort to developing new drugs against such diseases, since there are currently no effective drugs for neurodegenerative disease treatment. Promoting the capacity for nerve regeneration is an ideal treatment target. The present study aimed to investigate the neurotrophic effects of 7,8-dihydroxycoumarin （DHC） or daphnetin in primary cultured rat cortical neurons. Methods Cortical neurons were identified by microtubule-associated protein 2 （MAP2） immunostaining. Morphological observation was used to measure the average length of neurite outgrowth. MTT and lactate dehydrogenase assays were used to assess neuronal survival. The mRNA expression of MAP2 and brain-derived neurotrophic factor （BDNF） was measured by RT-PCR. Results MAP2 immunostaining showed that most of the cultured cells were neurons. Compared with the vehicle control group, DHC promoted neurite outgrowth and prolonged neuronal survival time at concentrations ranging from 2 to 8 μmol/L. Expression of both BDNF mRNA and MAP2 mRNAwas increased in the groups treated with 2, 4 and 8 μmol/L DHC. Conclusion DHC significantly increases neurite outgrowth and promotes neuronal survival in primary cultured rat cortical neurons. The neurotrophic effects of DHC are probably associated with increased BDNF expression.</description><subject>Anatomy</subject><subject>Anesthesiology</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Survival - drug effects</subject><subject>Cell Survival - physiology</subject><subject>Cerebral Cortex - cytology</subject><subject>Cerebral Cortex - drug effects</subject><subject>Cerebral Cortex - physiology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Human Physiology</subject><subject>mRNA表达</subject><subject>Nerve Growth Factors - chemistry</subject><subject>Nerve Growth Factors - pharmacology</subject><subject>Nerve Regeneration - drug effects</subject><subject>Nerve Regeneration - physiology</subject><subject>Neurology</subject><subject>Neurons - drug effects</subject><subject>Neurons - physiology</subject><subject>Neurosciences</subject><subject>Original</subject><subject>Original Article</subject><subject>Pain Medicine</subject><subject>Primary Cell Culture</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Umbelliferones - chemistry</subject><subject>Umbelliferones - pharmacology</subject><subject>原代培养</subject><subject>大鼠</subject><subject>皮层神经元</subject><subject>神经营养作用</subject><subject>神经退行性疾病</subject><subject>羟基香豆素</subject><subject>脑源性神经营养因子</subject><issn>1673-7067</issn><issn>1995-8218</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF1LwzAUhoMoTqc_wBup90aTtM3HjSDDLxh6o96GJE3Wjq2ZSSvu35vROfRGCOSQc94nhweAM4yuMELsOmJCaAERJhCTPIdsDxxhIUrICeb7qaYsPSLKRuA4xjlCFLG8OAQjQgTFBUNH4P3Z9sF3wa_qxmTWOWu6mHmXsUsOq6ZeV8F_rY3vlyo0bZbOKjSpXmemX3R9sFUWVJcZH7rGqEXWbnBtPAEHTi2iPd3eY_B2f_c6eYTTl4enye0UmoLyDhrMS0E0s7oiWhtKNTGcY84Y4ZbwgpdIC1UxwSqHnGNKOyO0cSwXFGGh8jG4GbirXi9tZWzbBbWQ2x2lV43822mbWs78pyxLikhZJAAeACb4GIN1uyxGciNZDpJlkiw3kiVLmfPfn-4SP1bTABkGYmq1Mxvk3PehTSL-pV5sN6l9O_tIuR24yDmjGNH8GxvjldE</recordid><startdate>20121001</startdate><enddate>20121001</enddate><creator>Yan, Li</creator><creator>Zhou, Xiaowen</creator><creator>Zhou, Xing</creator><creator>Zhang, Zheng</creator><creator>Luo, Huan-Min</creator><general>Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20121001</creationdate><title>Neurotrophic effects of 7,8-dihydroxycoumarin in primary cultured rat cortical neurons</title><author>Yan, Li ; Zhou, Xiaowen ; Zhou, Xing ; Zhang, Zheng ; Luo, Huan-Min</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-c18592b7ebd2bbc66b2c88187728e284850b9ad797df0ff7abfc9bcf7396019a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Anatomy</topic><topic>Anesthesiology</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell Survival - drug effects</topic><topic>Cell Survival - physiology</topic><topic>Cerebral Cortex - cytology</topic><topic>Cerebral Cortex - drug effects</topic><topic>Cerebral Cortex - physiology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Human Physiology</topic><topic>mRNA表达</topic><topic>Nerve Growth Factors - chemistry</topic><topic>Nerve Growth Factors - pharmacology</topic><topic>Nerve Regeneration - drug effects</topic><topic>Nerve Regeneration - physiology</topic><topic>Neurology</topic><topic>Neurons - drug effects</topic><topic>Neurons - physiology</topic><topic>Neurosciences</topic><topic>Original</topic><topic>Original Article</topic><topic>Pain Medicine</topic><topic>Primary Cell Culture</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Umbelliferones - chemistry</topic><topic>Umbelliferones - pharmacology</topic><topic>原代培养</topic><topic>大鼠</topic><topic>皮层神经元</topic><topic>神经营养作用</topic><topic>神经退行性疾病</topic><topic>羟基香豆素</topic><topic>脑源性神经营养因子</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yan, Li</creatorcontrib><creatorcontrib>Zhou, Xiaowen</creatorcontrib><creatorcontrib>Zhou, Xing</creatorcontrib><creatorcontrib>Zhang, Zheng</creatorcontrib><creatorcontrib>Luo, Huan-Min</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Neuroscience bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yan, Li</au><au>Zhou, Xiaowen</au><au>Zhou, Xing</au><au>Zhang, Zheng</au><au>Luo, Huan-Min</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neurotrophic effects of 7,8-dihydroxycoumarin in primary cultured rat cortical neurons</atitle><jtitle>Neuroscience bulletin</jtitle><stitle>Neurosci. Bull</stitle><addtitle>Neuroscience Bulletin</addtitle><date>2012-10-01</date><risdate>2012</risdate><volume>28</volume><issue>5</issue><spage>493</spage><epage>498</epage><pages>493-498</pages><issn>1673-7067</issn><eissn>1995-8218</eissn><abstract>Objective Neuronal loss in the central nervous system is central to the occurrence of neurodegenerative diseases. Pharmaceutical companies have devoted much effort to developing new drugs against such diseases, since there are currently no effective drugs for neurodegenerative disease treatment. Promoting the capacity for nerve regeneration is an ideal treatment target. The present study aimed to investigate the neurotrophic effects of 7,8-dihydroxycoumarin （DHC） or daphnetin in primary cultured rat cortical neurons. Methods Cortical neurons were identified by microtubule-associated protein 2 （MAP2） immunostaining. Morphological observation was used to measure the average length of neurite outgrowth. MTT and lactate dehydrogenase assays were used to assess neuronal survival. The mRNA expression of MAP2 and brain-derived neurotrophic factor （BDNF） was measured by RT-PCR. Results MAP2 immunostaining showed that most of the cultured cells were neurons. Compared with the vehicle control group, DHC promoted neurite outgrowth and prolonged neuronal survival time at concentrations ranging from 2 to 8 μmol/L. Expression of both BDNF mRNA and MAP2 mRNAwas increased in the groups treated with 2, 4 and 8 μmol/L DHC. Conclusion DHC significantly increases neurite outgrowth and promotes neuronal survival in primary cultured rat cortical neurons. The neurotrophic effects of DHC are probably associated with increased BDNF expression.</abstract><cop>Heidelberg</cop><pub>Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences</pub><pmid>22961470</pmid><doi>10.1007/s12264-012-1233-7</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Neuroscience bulletin, 2012-10, Vol.28 (5), p.493-498 |
| issn | 1673-7067 1995-8218 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Anatomy Anesthesiology Animals Animals, Newborn Biomedical and Life Sciences Biomedicine Cell Survival - drug effects Cell Survival - physiology Cerebral Cortex - cytology Cerebral Cortex - drug effects Cerebral Cortex - physiology Dose-Response Relationship, Drug Human Physiology mRNA表达 Nerve Growth Factors - chemistry Nerve Growth Factors - pharmacology Nerve Regeneration - drug effects Nerve Regeneration - physiology Neurology Neurons - drug effects Neurons - physiology Neurosciences Original Original Article Pain Medicine Primary Cell Culture Rats Rats, Sprague-Dawley Umbelliferones - chemistry Umbelliferones - pharmacology 原代培养 大鼠 皮层神经元 神经营养作用 神经退行性疾病 羟基香豆素 脑源性神经营养因子 |
| title | Neurotrophic effects of 7,8-dihydroxycoumarin in primary cultured rat cortical neurons |
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