Protease cleavage site fingerprinting by label‐free in‐gel degradomics reveals pH‐dependent specificity switch of legumain

Determination of protease specificity is of crucial importance for understanding protease function. We have developed the first gel‐based label‐free proteomic approach (DIPPS—direct in‐gel profiling of protease specificity) that enables quick and reliable determination of protease cleavage specifici...

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Veröffentlicht in:	The EMBO journal 2017-08, Vol.36 (16), p.2455-2465
Hauptverfasser:	Vidmar, Robert, Vizovišek, Matej, Turk, Dušan, Turk, Boris, Fonović, Marko
Format:	Artikel
Sprache:	eng
Schlagworte:	Caspase Caspase-7 Cathepsins Cleavage Cysteine Endopeptidases - metabolism Digestion Dipping Electrophoresis EMBO08 EMBO22 EMBO31 Fingerprinting Hydrogen-Ion Concentration Legumain Mass Spectrometry Mass spectroscopy Matrix metalloproteinase Metalloproteinase Peptides pH effects Protease Proteases Proteinase proteomics Proteomics - methods Resource specificity profiling Substrate Specificity Temperature Temperature effects Thermolysin Trypsin
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