Direct detection of extended-spectrum beta-lactamases (CTX-M) from blood cultures by LC-MS/MS bottom-up proteomics

Rapid bacterial species identification and antibiotic susceptibility testing in positive blood cultures have an important impact on the antibiotic treatment for patients. To identify extended-spectrum beta-lactamases (ESBL) directly in positive blood culture bottles, we developed a workflow of sapon...

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Veröffentlicht in:	European journal of clinical microbiology & infectious diseases 2017-09, Vol.36 (9), p.1621-1628
Hauptverfasser:	Fleurbaaij, F., Goessens, W., van Leeuwen, H. C., Kraakman, M. E. M., Bernards, S. T., Hensbergen, P. J., Kuijper, E. J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Anti-Bacterial Agents - pharmacology Antibiotics Bacteria beta-Lactamases - chemistry beta-Lactamases - genetics beta-Lactamases - metabolism Biomarkers Biomedical and Life Sciences Biomedicine Blood Blood Culture Chromatography, Liquid E coli Escherichia coli - drug effects Escherichia coli - genetics Escherichia coli Infections - diagnosis Escherichia coli Infections - drug therapy Escherichia coli Infections - microbiology Faith healing Humans Internal Medicine Klebsiella Klebsiella Infections - diagnosis Klebsiella Infections - drug therapy Klebsiella Infections - microbiology Klebsiella pneumoniae Klebsiella pneumoniae - drug effects Klebsiella pneumoniae - genetics Liquid chromatography Mass spectrometry Mass spectroscopy Medical Microbiology Microbial Sensitivity Tests Original Original Article Prayer Prospective Studies Proteomics Proteomics - methods Sequence Analysis, DNA Workflow
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