Macrophage type modulates osteogenic differentiation of adipose tissue MSCs
Since the reconstruction of large bone defects remains a challenge, knowledge about the biology of bone healing is desirable to develop novel strategies for improving the treatment of bone defects. In osteoimmunology, macrophages are the central component in the early stage of physiological response...
Gespeichert in:
| Veröffentlicht in: | Cell and tissue research 2017-08, Vol.369 (2), p.273-286 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 286 |
|---|---|
| container_issue | 2 |
| container_start_page | 273 |
| container_title | Cell and tissue research |
| container_volume | 369 |
| creator | Zhang, Yang Böse, Thomas Unger, Ronald E. Jansen, John A. Kirkpatrick, Charles James van den Beucken, Jeroen J. J. P. |
| description | Since the reconstruction of large bone defects remains a challenge, knowledge about the biology of bone healing is desirable to develop novel strategies for improving the treatment of bone defects. In osteoimmunology, macrophages are the central component in the early stage of physiological response after bone injury and bone remodeling in the late stage. During this process, a switch of macrophage phenotype from pro-inflammatory (M1) to anti-inflammatory (M2) is observed. An appealing option for bone regeneration would be to exploit this regulatory role for the benefit of osteogenic differentiation of osteoprogenitor cells (e.g., mesenchymal stem cells; MSCs) and to eventually utilize this knowledge to improve the therapeutic outcome of bone regenerative treatment. In view of this, we focused on the in vitro interaction of different macrophage subtypes with adipose tissue MSCs to monitor the behavior (i.e. proliferation, differentiation and mineralization) of the latter in dedicated co-culture models. Our data show that co-culture of MSCs with M2 macrophages, but not with M1 macrophages or M0 macrophages, results in significantly increased MSC mineralization caused by soluble factors. Specifically, M2 macrophages promoted the proliferation and osteogenic differentiation of MSCs, while M0 and M1 macrophages solely stimulated the osteogenic differentiation of MSCs in the early and middle stages during co-culture. Secretion of the soluble factors oncostatin M (OSM) and bone morphogenetic protein 2 (BMP-2) by macrophages showed correlation with MSC gene expression levels for OSM-receptor and BMP-2, suggesting the involvement of both signaling pathways in the osteogenic differentiation of MSCs. |
| doi_str_mv | 10.1007/s00441-017-2598-8 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5552848</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A501298180</galeid><sourcerecordid>A501298180</sourcerecordid><originalsourceid>FETCH-LOGICAL-c601t-80b95bfa6a040348c3cae6e53ab0fc3bc4444abad01f345ff59e7984ec9924cb3</originalsourceid><addsrcrecordid>eNqFkk-L1TAUxYsoznP0A7iRgiCz6Xjzr0k3wvBwVJzBhQruQpre9GVom9q0wnx7U944vieKySKQ-zuHm5uTZc8JnBMA-ToCcE4KILKgolKFepBtCGe0ACXVw2wDDGghy_LbSfYkxhsAwsuyepydUMVKwoBtso_Xxk5h3JkW8_l2xLwPzdKZGWMe4oyhxcHbvPHO4YTD7M3sw5AHl5vGjyEmkY9xwfz68zY-zR4500V8dneeZl8v337Zvi-uPr37sL24KmwJZC4U1JWonSkNcGBcWWYNliiYqcFZVluelqlNA8QxLpwTFcpKcbRVRbmt2Wn2Zu87LnWPjU19TabT4-R7M93qYLw-rgx-p9vwQwshqOIqGZzdGUzh-4Jx1r2PFrvODBiWqCkACKYkyP-iRClGZEkrSOjLP9CbsExDmoQmFZVcUuDkN9WaDrUfXEgt2tVUXwggtFJErV7nf6HSbrD3NgzofLo_Erw6EOzQdPMuhm5Zvyseg2QPpm-PcUJ3PzcCek2V3qdKp1TpNVV6ndeLw4HfK37FKAF0D8RUGlqcDp7-T9ef-_TWDg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1927472041</pqid></control><display><type>article</type><title>Macrophage type modulates osteogenic differentiation of adipose tissue MSCs</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Zhang, Yang ; Böse, Thomas ; Unger, Ronald E. ; Jansen, John A. ; Kirkpatrick, Charles James ; van den Beucken, Jeroen J. J. P.</creator><creatorcontrib>Zhang, Yang ; Böse, Thomas ; Unger, Ronald E. ; Jansen, John A. ; Kirkpatrick, Charles James ; van den Beucken, Jeroen J. J. P.</creatorcontrib><description>Since the reconstruction of large bone defects remains a challenge, knowledge about the biology of bone healing is desirable to develop novel strategies for improving the treatment of bone defects. In osteoimmunology, macrophages are the central component in the early stage of physiological response after bone injury and bone remodeling in the late stage. During this process, a switch of macrophage phenotype from pro-inflammatory (M1) to anti-inflammatory (M2) is observed. An appealing option for bone regeneration would be to exploit this regulatory role for the benefit of osteogenic differentiation of osteoprogenitor cells (e.g., mesenchymal stem cells; MSCs) and to eventually utilize this knowledge to improve the therapeutic outcome of bone regenerative treatment. In view of this, we focused on the in vitro interaction of different macrophage subtypes with adipose tissue MSCs to monitor the behavior (i.e. proliferation, differentiation and mineralization) of the latter in dedicated co-culture models. Our data show that co-culture of MSCs with M2 macrophages, but not with M1 macrophages or M0 macrophages, results in significantly increased MSC mineralization caused by soluble factors. Specifically, M2 macrophages promoted the proliferation and osteogenic differentiation of MSCs, while M0 and M1 macrophages solely stimulated the osteogenic differentiation of MSCs in the early and middle stages during co-culture. Secretion of the soluble factors oncostatin M (OSM) and bone morphogenetic protein 2 (BMP-2) by macrophages showed correlation with MSC gene expression levels for OSM-receptor and BMP-2, suggesting the involvement of both signaling pathways in the osteogenic differentiation of MSCs.</description><identifier>ISSN: 0302-766X</identifier><identifier>EISSN: 1432-0878</identifier><identifier>DOI: 10.1007/s00441-017-2598-8</identifier><identifier>PMID: 28361303</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Adipose tissue ; Adipose Tissue - cytology ; Alkaline Phosphatase - metabolism ; Biomarkers - metabolism ; Biomedical and Life Sciences ; Biomedicine ; bone formation ; Bone growth ; Bone healing ; Bone morphogenetic protein 2 ; Bone morphogenetic proteins ; Bone remodeling ; Calcification, Physiologic ; Cell Communication ; Cell culture ; Cell Differentiation - genetics ; Cell Line ; Cell Polarity ; Cell Proliferation ; coculture ; Coculture Techniques ; Cytokines - metabolism ; Gene expression ; Gene Expression Regulation ; Human Genetics ; Humans ; Immunology ; Inflammation ; Macrophages ; Macrophages - cytology ; Macrophages - metabolism ; Mesenchymal Stem Cells - cytology ; Mesenchymal Stem Cells - enzymology ; Mesenchyme ; Mineralization ; Molecular Medicine ; Oncostatin M ; Osteogenesis - genetics ; Osteoprogenitor cells ; phenotype ; physiological response ; Proteomics ; Reconstruction ; Regeneration ; Regular ; Regular Article ; Secretion ; signal transduction ; Skin & tissue grafts ; Stem cells</subject><ispartof>Cell and tissue research, 2017-08, Vol.369 (2), p.273-286</ispartof><rights>The Author(s) 2017</rights><rights>COPYRIGHT 2017 Springer</rights><rights>Cell and Tissue Research is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c601t-80b95bfa6a040348c3cae6e53ab0fc3bc4444abad01f345ff59e7984ec9924cb3</citedby><cites>FETCH-LOGICAL-c601t-80b95bfa6a040348c3cae6e53ab0fc3bc4444abad01f345ff59e7984ec9924cb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00441-017-2598-8$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00441-017-2598-8$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,777,781,882,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28361303$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Yang</creatorcontrib><creatorcontrib>Böse, Thomas</creatorcontrib><creatorcontrib>Unger, Ronald E.</creatorcontrib><creatorcontrib>Jansen, John A.</creatorcontrib><creatorcontrib>Kirkpatrick, Charles James</creatorcontrib><creatorcontrib>van den Beucken, Jeroen J. J. P.</creatorcontrib><title>Macrophage type modulates osteogenic differentiation of adipose tissue MSCs</title><title>Cell and tissue research</title><addtitle>Cell Tissue Res</addtitle><addtitle>Cell Tissue Res</addtitle><description>Since the reconstruction of large bone defects remains a challenge, knowledge about the biology of bone healing is desirable to develop novel strategies for improving the treatment of bone defects. In osteoimmunology, macrophages are the central component in the early stage of physiological response after bone injury and bone remodeling in the late stage. During this process, a switch of macrophage phenotype from pro-inflammatory (M1) to anti-inflammatory (M2) is observed. An appealing option for bone regeneration would be to exploit this regulatory role for the benefit of osteogenic differentiation of osteoprogenitor cells (e.g., mesenchymal stem cells; MSCs) and to eventually utilize this knowledge to improve the therapeutic outcome of bone regenerative treatment. In view of this, we focused on the in vitro interaction of different macrophage subtypes with adipose tissue MSCs to monitor the behavior (i.e. proliferation, differentiation and mineralization) of the latter in dedicated co-culture models. Our data show that co-culture of MSCs with M2 macrophages, but not with M1 macrophages or M0 macrophages, results in significantly increased MSC mineralization caused by soluble factors. Specifically, M2 macrophages promoted the proliferation and osteogenic differentiation of MSCs, while M0 and M1 macrophages solely stimulated the osteogenic differentiation of MSCs in the early and middle stages during co-culture. Secretion of the soluble factors oncostatin M (OSM) and bone morphogenetic protein 2 (BMP-2) by macrophages showed correlation with MSC gene expression levels for OSM-receptor and BMP-2, suggesting the involvement of both signaling pathways in the osteogenic differentiation of MSCs.</description><subject>Adipose tissue</subject><subject>Adipose Tissue - cytology</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Biomarkers - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>bone formation</subject><subject>Bone growth</subject><subject>Bone healing</subject><subject>Bone morphogenetic protein 2</subject><subject>Bone morphogenetic proteins</subject><subject>Bone remodeling</subject><subject>Calcification, Physiologic</subject><subject>Cell Communication</subject><subject>Cell culture</subject><subject>Cell Differentiation - genetics</subject><subject>Cell Line</subject><subject>Cell Polarity</subject><subject>Cell Proliferation</subject><subject>coculture</subject><subject>Coculture Techniques</subject><subject>Cytokines - metabolism</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>Immunology</subject><subject>Inflammation</subject><subject>Macrophages</subject><subject>Macrophages - cytology</subject><subject>Macrophages - metabolism</subject><subject>Mesenchymal Stem Cells - cytology</subject><subject>Mesenchymal Stem Cells - enzymology</subject><subject>Mesenchyme</subject><subject>Mineralization</subject><subject>Molecular Medicine</subject><subject>Oncostatin M</subject><subject>Osteogenesis - genetics</subject><subject>Osteoprogenitor cells</subject><subject>phenotype</subject><subject>physiological response</subject><subject>Proteomics</subject><subject>Reconstruction</subject><subject>Regeneration</subject><subject>Regular</subject><subject>Regular Article</subject><subject>Secretion</subject><subject>signal transduction</subject><subject>Skin & tissue grafts</subject><subject>Stem cells</subject><issn>0302-766X</issn><issn>1432-0878</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkk-L1TAUxYsoznP0A7iRgiCz6Xjzr0k3wvBwVJzBhQruQpre9GVom9q0wnx7U944vieKySKQ-zuHm5uTZc8JnBMA-ToCcE4KILKgolKFepBtCGe0ACXVw2wDDGghy_LbSfYkxhsAwsuyepydUMVKwoBtso_Xxk5h3JkW8_l2xLwPzdKZGWMe4oyhxcHbvPHO4YTD7M3sw5AHl5vGjyEmkY9xwfz68zY-zR4500V8dneeZl8v337Zvi-uPr37sL24KmwJZC4U1JWonSkNcGBcWWYNliiYqcFZVluelqlNA8QxLpwTFcpKcbRVRbmt2Wn2Zu87LnWPjU19TabT4-R7M93qYLw-rgx-p9vwQwshqOIqGZzdGUzh-4Jx1r2PFrvODBiWqCkACKYkyP-iRClGZEkrSOjLP9CbsExDmoQmFZVcUuDkN9WaDrUfXEgt2tVUXwggtFJErV7nf6HSbrD3NgzofLo_Erw6EOzQdPMuhm5Zvyseg2QPpm-PcUJ3PzcCek2V3qdKp1TpNVV6ndeLw4HfK37FKAF0D8RUGlqcDp7-T9ef-_TWDg</recordid><startdate>20170801</startdate><enddate>20170801</enddate><creator>Zhang, Yang</creator><creator>Böse, Thomas</creator><creator>Unger, Ronald E.</creator><creator>Jansen, John A.</creator><creator>Kirkpatrick, Charles James</creator><creator>van den Beucken, Jeroen J. J. P.</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7RV</scope><scope>7SS</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope></search><sort><creationdate>20170801</creationdate><title>Macrophage type modulates osteogenic differentiation of adipose tissue MSCs</title><author>Zhang, Yang ; Böse, Thomas ; Unger, Ronald E. ; Jansen, John A. ; Kirkpatrick, Charles James ; van den Beucken, Jeroen J. J. P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c601t-80b95bfa6a040348c3cae6e53ab0fc3bc4444abad01f345ff59e7984ec9924cb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adipose tissue</topic><topic>Adipose Tissue - cytology</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>Biomarkers - metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>bone formation</topic><topic>Bone growth</topic><topic>Bone healing</topic><topic>Bone morphogenetic protein 2</topic><topic>Bone morphogenetic proteins</topic><topic>Bone remodeling</topic><topic>Calcification, Physiologic</topic><topic>Cell Communication</topic><topic>Cell culture</topic><topic>Cell Differentiation - genetics</topic><topic>Cell Line</topic><topic>Cell Polarity</topic><topic>Cell Proliferation</topic><topic>coculture</topic><topic>Coculture Techniques</topic><topic>Cytokines - metabolism</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>Immunology</topic><topic>Inflammation</topic><topic>Macrophages</topic><topic>Macrophages - cytology</topic><topic>Macrophages - metabolism</topic><topic>Mesenchymal Stem Cells - cytology</topic><topic>Mesenchymal Stem Cells - enzymology</topic><topic>Mesenchyme</topic><topic>Mineralization</topic><topic>Molecular Medicine</topic><topic>Oncostatin M</topic><topic>Osteogenesis - genetics</topic><topic>Osteoprogenitor cells</topic><topic>phenotype</topic><topic>physiological response</topic><topic>Proteomics</topic><topic>Reconstruction</topic><topic>Regeneration</topic><topic>Regular</topic><topic>Regular Article</topic><topic>Secretion</topic><topic>signal transduction</topic><topic>Skin & tissue grafts</topic><topic>Stem cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Yang</creatorcontrib><creatorcontrib>Böse, Thomas</creatorcontrib><creatorcontrib>Unger, Ronald E.</creatorcontrib><creatorcontrib>Jansen, John A.</creatorcontrib><creatorcontrib>Kirkpatrick, Charles James</creatorcontrib><creatorcontrib>van den Beucken, Jeroen J. J. P.</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cell and tissue research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Yang</au><au>Böse, Thomas</au><au>Unger, Ronald E.</au><au>Jansen, John A.</au><au>Kirkpatrick, Charles James</au><au>van den Beucken, Jeroen J. J. P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Macrophage type modulates osteogenic differentiation of adipose tissue MSCs</atitle><jtitle>Cell and tissue research</jtitle><stitle>Cell Tissue Res</stitle><addtitle>Cell Tissue Res</addtitle><date>2017-08-01</date><risdate>2017</risdate><volume>369</volume><issue>2</issue><spage>273</spage><epage>286</epage><pages>273-286</pages><issn>0302-766X</issn><eissn>1432-0878</eissn><abstract>Since the reconstruction of large bone defects remains a challenge, knowledge about the biology of bone healing is desirable to develop novel strategies for improving the treatment of bone defects. In osteoimmunology, macrophages are the central component in the early stage of physiological response after bone injury and bone remodeling in the late stage. During this process, a switch of macrophage phenotype from pro-inflammatory (M1) to anti-inflammatory (M2) is observed. An appealing option for bone regeneration would be to exploit this regulatory role for the benefit of osteogenic differentiation of osteoprogenitor cells (e.g., mesenchymal stem cells; MSCs) and to eventually utilize this knowledge to improve the therapeutic outcome of bone regenerative treatment. In view of this, we focused on the in vitro interaction of different macrophage subtypes with adipose tissue MSCs to monitor the behavior (i.e. proliferation, differentiation and mineralization) of the latter in dedicated co-culture models. Our data show that co-culture of MSCs with M2 macrophages, but not with M1 macrophages or M0 macrophages, results in significantly increased MSC mineralization caused by soluble factors. Specifically, M2 macrophages promoted the proliferation and osteogenic differentiation of MSCs, while M0 and M1 macrophages solely stimulated the osteogenic differentiation of MSCs in the early and middle stages during co-culture. Secretion of the soluble factors oncostatin M (OSM) and bone morphogenetic protein 2 (BMP-2) by macrophages showed correlation with MSC gene expression levels for OSM-receptor and BMP-2, suggesting the involvement of both signaling pathways in the osteogenic differentiation of MSCs.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>28361303</pmid><doi>10.1007/s00441-017-2598-8</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0302-766X |
| ispartof | Cell and tissue research, 2017-08, Vol.369 (2), p.273-286 |
| issn | 0302-766X 1432-0878 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5552848 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Adipose tissue Adipose Tissue - cytology Alkaline Phosphatase - metabolism Biomarkers - metabolism Biomedical and Life Sciences Biomedicine bone formation Bone growth Bone healing Bone morphogenetic protein 2 Bone morphogenetic proteins Bone remodeling Calcification, Physiologic Cell Communication Cell culture Cell Differentiation - genetics Cell Line Cell Polarity Cell Proliferation coculture Coculture Techniques Cytokines - metabolism Gene expression Gene Expression Regulation Human Genetics Humans Immunology Inflammation Macrophages Macrophages - cytology Macrophages - metabolism Mesenchymal Stem Cells - cytology Mesenchymal Stem Cells - enzymology Mesenchyme Mineralization Molecular Medicine Oncostatin M Osteogenesis - genetics Osteoprogenitor cells phenotype physiological response Proteomics Reconstruction Regeneration Regular Regular Article Secretion signal transduction Skin & tissue grafts Stem cells |
| title | Macrophage type modulates osteogenic differentiation of adipose tissue MSCs |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T05%3A51%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Macrophage%20type%20modulates%20osteogenic%20differentiation%20of%20adipose%20tissue%20MSCs&rft.jtitle=Cell%20and%20tissue%20research&rft.au=Zhang,%20Yang&rft.date=2017-08-01&rft.volume=369&rft.issue=2&rft.spage=273&rft.epage=286&rft.pages=273-286&rft.issn=0302-766X&rft.eissn=1432-0878&rft_id=info:doi/10.1007/s00441-017-2598-8&rft_dat=%3Cgale_pubme%3EA501298180%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1927472041&rft_id=info:pmid/28361303&rft_galeid=A501298180&rfr_iscdi=true |