Spearmint R2R3‐MYB transcription factor MsMYB negatively regulates monoterpene production and suppresses the expression of geranyl diphosphate synthase large subunit (MsGPPS.LSU)
Summary Many aromatic plants, such as spearmint, produce valuable essential oils in specialized structures called peltate glandular trichomes (PGTs). Understanding the regulatory mechanisms behind the production of these important secondary metabolites will help design new approaches to engineer the...
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| Veröffentlicht in: | Plant biotechnology journal 2017-09, Vol.15 (9), p.1105-1119 |
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| creator | Reddy, Vaishnavi Amarr Wang, Qian Dhar, Niha Kumar, Nadimuthu Venkatesh, Prasanna Nori Rajan, Chakravarthy Panicker, Deepa Sridhar, Vishweshwaran Mao, Hui‐Zhu Sarojam, Rajani |
| description | Summary
Many aromatic plants, such as spearmint, produce valuable essential oils in specialized structures called peltate glandular trichomes (PGTs). Understanding the regulatory mechanisms behind the production of these important secondary metabolites will help design new approaches to engineer them. Here, we identified a PGT‐specific R2R3‐MYB gene, MsMYB, from comparative RNA‐Seq data of spearmint and functionally characterized it. Analysis of MsMYB‐RNAi transgenic lines showed increased levels of monoterpenes, and MsMYB‐overexpressing lines exhibited decreased levels of monoterpenes. These results suggest that MsMYB is a novel negative regulator of monoterpene biosynthesis. Ectopic expression of MsMYB, in sweet basil and tobacco, perturbed sesquiterpene‐ and diterpene‐derived metabolite production. In addition, we found that MsMYB binds to cis‐elements of MsGPPS.LSU and suppresses its expression. Phylogenetic analysis placed MsMYB in subgroup 7 of R2R3‐MYBs whose members govern phenylpropanoid pathway and are regulated by miR858. Analysis of transgenic lines showed that MsMYB is more specific to terpene biosynthesis as it did not affect metabolites derived from phenylpropanoid pathway. Further, our results indicate that MsMYB is probably not regulated by miR858, like other members of subgroup 7. |
| doi_str_mv | 10.1111/pbi.12701 |
| format | Article |
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Many aromatic plants, such as spearmint, produce valuable essential oils in specialized structures called peltate glandular trichomes (PGTs). Understanding the regulatory mechanisms behind the production of these important secondary metabolites will help design new approaches to engineer them. Here, we identified a PGT‐specific R2R3‐MYB gene, MsMYB, from comparative RNA‐Seq data of spearmint and functionally characterized it. Analysis of MsMYB‐RNAi transgenic lines showed increased levels of monoterpenes, and MsMYB‐overexpressing lines exhibited decreased levels of monoterpenes. These results suggest that MsMYB is a novel negative regulator of monoterpene biosynthesis. Ectopic expression of MsMYB, in sweet basil and tobacco, perturbed sesquiterpene‐ and diterpene‐derived metabolite production. In addition, we found that MsMYB binds to cis‐elements of MsGPPS.LSU and suppresses its expression. Phylogenetic analysis placed MsMYB in subgroup 7 of R2R3‐MYBs whose members govern phenylpropanoid pathway and are regulated by miR858. Analysis of transgenic lines showed that MsMYB is more specific to terpene biosynthesis as it did not affect metabolites derived from phenylpropanoid pathway. Further, our results indicate that MsMYB is probably not regulated by miR858, like other members of subgroup 7.</description><identifier>ISSN: 1467-7644</identifier><identifier>EISSN: 1467-7652</identifier><identifier>DOI: 10.1111/pbi.12701</identifier><identifier>PMID: 28160379</identifier><language>eng</language><publisher>England: John Wiley & Sons, Inc</publisher><subject>Amino Acid Sequence ; Base Sequence ; Biosynthesis ; Comparative analysis ; Design engineering ; Diphosphates - metabolism ; Diterpenes - metabolism ; Ectopic expression ; essential oil crops ; Essential oils ; Gene Expression ; Gene Expression Regulation, Plant ; gene overexpression ; genes ; Genetic engineering ; genetically modified organisms ; Geranyltranstransferase - genetics ; Geranyltranstransferase - metabolism ; GPPS ; Mentha spicata ; Mentha spicata - cytology ; Mentha spicata - genetics ; Mentha spicata - metabolism ; Metabolites ; Monoterpenes ; Monoterpenes - metabolism ; monoterpenoids ; MYB gene ; Nicotiana - cytology ; Nicotiana - genetics ; Nicotiana - metabolism ; Ocimum basilicum ; Ocimum basilicum - cytology ; Ocimum basilicum - genetics ; Ocimum basilicum - metabolism ; Oils, Volatile - metabolism ; Phylogeny ; Physiological aspects ; Plant Leaves - genetics ; Plant Leaves - metabolism ; Plant metabolites ; Plant Oils - metabolism ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants ; Plants (botany) ; Plants, Genetically Modified ; R2R3‐MYB ; Regulatory mechanisms (biology) ; Ribonucleic acid ; RNA ; RNA-mediated interference ; Secondary Metabolism ; Secondary metabolites ; sequence analysis ; Sesquiterpenes - metabolism ; spearmint ; terpene ; Tobacco ; transcription factor ; transcription factors ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Trichomes</subject><ispartof>Plant biotechnology journal, 2017-09, Vol.15 (9), p.1105-1119</ispartof><rights>2017 The Authors. published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.</rights><rights>2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.</rights><rights>COPYRIGHT 2017 John Wiley & Sons, Inc.</rights><rights>2017. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0003-0553-272X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fpbi.12701$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fpbi.12701$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,860,881,1411,11541,27901,27902,45550,45551,46027,46451</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28160379$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Reddy, Vaishnavi Amarr</creatorcontrib><creatorcontrib>Wang, Qian</creatorcontrib><creatorcontrib>Dhar, Niha</creatorcontrib><creatorcontrib>Kumar, Nadimuthu</creatorcontrib><creatorcontrib>Venkatesh, Prasanna Nori</creatorcontrib><creatorcontrib>Rajan, Chakravarthy</creatorcontrib><creatorcontrib>Panicker, Deepa</creatorcontrib><creatorcontrib>Sridhar, Vishweshwaran</creatorcontrib><creatorcontrib>Mao, Hui‐Zhu</creatorcontrib><creatorcontrib>Sarojam, Rajani</creatorcontrib><title>Spearmint R2R3‐MYB transcription factor MsMYB negatively regulates monoterpene production and suppresses the expression of geranyl diphosphate synthase large subunit (MsGPPS.LSU)</title><title>Plant biotechnology journal</title><addtitle>Plant Biotechnol J</addtitle><description>Summary
Many aromatic plants, such as spearmint, produce valuable essential oils in specialized structures called peltate glandular trichomes (PGTs). Understanding the regulatory mechanisms behind the production of these important secondary metabolites will help design new approaches to engineer them. Here, we identified a PGT‐specific R2R3‐MYB gene, MsMYB, from comparative RNA‐Seq data of spearmint and functionally characterized it. Analysis of MsMYB‐RNAi transgenic lines showed increased levels of monoterpenes, and MsMYB‐overexpressing lines exhibited decreased levels of monoterpenes. These results suggest that MsMYB is a novel negative regulator of monoterpene biosynthesis. Ectopic expression of MsMYB, in sweet basil and tobacco, perturbed sesquiterpene‐ and diterpene‐derived metabolite production. In addition, we found that MsMYB binds to cis‐elements of MsGPPS.LSU and suppresses its expression. Phylogenetic analysis placed MsMYB in subgroup 7 of R2R3‐MYBs whose members govern phenylpropanoid pathway and are regulated by miR858. Analysis of transgenic lines showed that MsMYB is more specific to terpene biosynthesis as it did not affect metabolites derived from phenylpropanoid pathway. Further, our results indicate that MsMYB is probably not regulated by miR858, like other members of subgroup 7.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Biosynthesis</subject><subject>Comparative analysis</subject><subject>Design engineering</subject><subject>Diphosphates - metabolism</subject><subject>Diterpenes - metabolism</subject><subject>Ectopic expression</subject><subject>essential oil crops</subject><subject>Essential oils</subject><subject>Gene Expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>gene overexpression</subject><subject>genes</subject><subject>Genetic engineering</subject><subject>genetically modified organisms</subject><subject>Geranyltranstransferase - genetics</subject><subject>Geranyltranstransferase - metabolism</subject><subject>GPPS</subject><subject>Mentha spicata</subject><subject>Mentha spicata - cytology</subject><subject>Mentha spicata - genetics</subject><subject>Mentha spicata - metabolism</subject><subject>Metabolites</subject><subject>Monoterpenes</subject><subject>Monoterpenes - metabolism</subject><subject>monoterpenoids</subject><subject>MYB gene</subject><subject>Nicotiana - cytology</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - metabolism</subject><subject>Ocimum basilicum</subject><subject>Ocimum basilicum - cytology</subject><subject>Ocimum basilicum - genetics</subject><subject>Ocimum basilicum - metabolism</subject><subject>Oils, Volatile - metabolism</subject><subject>Phylogeny</subject><subject>Physiological aspects</subject><subject>Plant Leaves - genetics</subject><subject>Plant Leaves - metabolism</subject><subject>Plant metabolites</subject><subject>Plant Oils - metabolism</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plants</subject><subject>Plants (botany)</subject><subject>Plants, Genetically Modified</subject><subject>R2R3‐MYB</subject><subject>Regulatory mechanisms (biology)</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA-mediated interference</subject><subject>Secondary Metabolism</subject><subject>Secondary metabolites</subject><subject>sequence analysis</subject><subject>Sesquiterpenes - metabolism</subject><subject>spearmint</subject><subject>terpene</subject><subject>Tobacco</subject><subject>transcription factor</subject><subject>transcription factors</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Trichomes</subject><issn>1467-7644</issn><issn>1467-7652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFUs1u1DAQjhCIlsKBF0CWuJTDbv2TOJsLUltBqbQrqi49cLKcZJx1ldiu7RT2xiPwMDwRT4J3t6yAC_bBM_6-GXs-fVn2kuApSevE1XpKaInJo-yQ5LyclLygj_dxnh9kz0K4xZgSXvCn2QGdEY5ZWR1mP5YOpB-0ieiaXrOf374vPp-h6KUJjdcuamuQkk20Hi3CBjLQyajvoV8jD93YywgBDdbYCN6BAeS8bcdmWyhNi8LonIcQEiuuAMHXbbZBrUIdpIfWPWq1W9ngVqkZCmsTVzIA6qXvUjrWo9ERHS_CxdXVcjpf3rx5nj1Rsg_w4uE8ym7ev_t0_mEy_3hxeX46n3R5TsikUnlZA24rSlrgHFOZU66AqKYlhWwqylkSR7V1JXlSpKyV4iU0laxAtpzU7Ch7u-vrxnqAtgGThOmF83qQfi2s1OJvxOiV6Oy9KIqC5rMiNTh-aODt3QghikGHBvpeGrBjEBRjXOSY4uq_VDLjRcEqhlmivv6HemtHb5ISglS0TJNiNkus6Y7VyR6ENsqmLzZptzDoxhpQOt2flozRnHFOUsGrP6fdj_nbLIlwsiN8SZXrPU6w2LhQJBeKrQvF1dnlNmC_ALR30-k</recordid><startdate>201709</startdate><enddate>201709</enddate><creator>Reddy, Vaishnavi Amarr</creator><creator>Wang, Qian</creator><creator>Dhar, Niha</creator><creator>Kumar, Nadimuthu</creator><creator>Venkatesh, Prasanna Nori</creator><creator>Rajan, Chakravarthy</creator><creator>Panicker, Deepa</creator><creator>Sridhar, Vishweshwaran</creator><creator>Mao, Hui‐Zhu</creator><creator>Sarojam, Rajani</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>LK8</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-0553-272X</orcidid></search><sort><creationdate>201709</creationdate><title>Spearmint R2R3‐MYB transcription factor MsMYB negatively regulates monoterpene production and suppresses the expression of geranyl diphosphate synthase large subunit (MsGPPS.LSU)</title><author>Reddy, Vaishnavi Amarr ; Wang, Qian ; Dhar, Niha ; Kumar, Nadimuthu ; Venkatesh, Prasanna Nori ; Rajan, Chakravarthy ; Panicker, Deepa ; Sridhar, Vishweshwaran ; Mao, Hui‐Zhu ; Sarojam, Rajani</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g4411-9f47be0d921de6602a426fe1fcd15ac9263764fdb9a62817bff67ec9a9ead61b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Biosynthesis</topic><topic>Comparative analysis</topic><topic>Design engineering</topic><topic>Diphosphates - metabolism</topic><topic>Diterpenes - metabolism</topic><topic>Ectopic expression</topic><topic>essential oil crops</topic><topic>Essential oils</topic><topic>Gene Expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>gene overexpression</topic><topic>genes</topic><topic>Genetic engineering</topic><topic>genetically modified organisms</topic><topic>Geranyltranstransferase - genetics</topic><topic>Geranyltranstransferase - metabolism</topic><topic>GPPS</topic><topic>Mentha spicata</topic><topic>Mentha spicata - cytology</topic><topic>Mentha spicata - genetics</topic><topic>Mentha spicata - metabolism</topic><topic>Metabolites</topic><topic>Monoterpenes</topic><topic>Monoterpenes - metabolism</topic><topic>monoterpenoids</topic><topic>MYB gene</topic><topic>Nicotiana - cytology</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - metabolism</topic><topic>Ocimum basilicum</topic><topic>Ocimum basilicum - cytology</topic><topic>Ocimum basilicum - genetics</topic><topic>Ocimum basilicum - metabolism</topic><topic>Oils, Volatile - metabolism</topic><topic>Phylogeny</topic><topic>Physiological aspects</topic><topic>Plant Leaves - genetics</topic><topic>Plant Leaves - metabolism</topic><topic>Plant metabolites</topic><topic>Plant Oils - metabolism</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plants</topic><topic>Plants (botany)</topic><topic>Plants, Genetically Modified</topic><topic>R2R3‐MYB</topic><topic>Regulatory mechanisms (biology)</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA-mediated interference</topic><topic>Secondary Metabolism</topic><topic>Secondary metabolites</topic><topic>sequence analysis</topic><topic>Sesquiterpenes - metabolism</topic><topic>spearmint</topic><topic>terpene</topic><topic>Tobacco</topic><topic>transcription factor</topic><topic>transcription factors</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Trichomes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reddy, Vaishnavi Amarr</creatorcontrib><creatorcontrib>Wang, Qian</creatorcontrib><creatorcontrib>Dhar, Niha</creatorcontrib><creatorcontrib>Kumar, Nadimuthu</creatorcontrib><creatorcontrib>Venkatesh, Prasanna Nori</creatorcontrib><creatorcontrib>Rajan, Chakravarthy</creatorcontrib><creatorcontrib>Panicker, Deepa</creatorcontrib><creatorcontrib>Sridhar, Vishweshwaran</creatorcontrib><creatorcontrib>Mao, Hui‐Zhu</creatorcontrib><creatorcontrib>Sarojam, Rajani</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant biotechnology journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reddy, Vaishnavi Amarr</au><au>Wang, Qian</au><au>Dhar, Niha</au><au>Kumar, Nadimuthu</au><au>Venkatesh, Prasanna Nori</au><au>Rajan, Chakravarthy</au><au>Panicker, Deepa</au><au>Sridhar, Vishweshwaran</au><au>Mao, Hui‐Zhu</au><au>Sarojam, Rajani</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spearmint R2R3‐MYB transcription factor MsMYB negatively regulates monoterpene production and suppresses the expression of geranyl diphosphate synthase large subunit (MsGPPS.LSU)</atitle><jtitle>Plant biotechnology journal</jtitle><addtitle>Plant Biotechnol J</addtitle><date>2017-09</date><risdate>2017</risdate><volume>15</volume><issue>9</issue><spage>1105</spage><epage>1119</epage><pages>1105-1119</pages><issn>1467-7644</issn><eissn>1467-7652</eissn><abstract>Summary
Many aromatic plants, such as spearmint, produce valuable essential oils in specialized structures called peltate glandular trichomes (PGTs). Understanding the regulatory mechanisms behind the production of these important secondary metabolites will help design new approaches to engineer them. Here, we identified a PGT‐specific R2R3‐MYB gene, MsMYB, from comparative RNA‐Seq data of spearmint and functionally characterized it. Analysis of MsMYB‐RNAi transgenic lines showed increased levels of monoterpenes, and MsMYB‐overexpressing lines exhibited decreased levels of monoterpenes. These results suggest that MsMYB is a novel negative regulator of monoterpene biosynthesis. Ectopic expression of MsMYB, in sweet basil and tobacco, perturbed sesquiterpene‐ and diterpene‐derived metabolite production. In addition, we found that MsMYB binds to cis‐elements of MsGPPS.LSU and suppresses its expression. Phylogenetic analysis placed MsMYB in subgroup 7 of R2R3‐MYBs whose members govern phenylpropanoid pathway and are regulated by miR858. Analysis of transgenic lines showed that MsMYB is more specific to terpene biosynthesis as it did not affect metabolites derived from phenylpropanoid pathway. Further, our results indicate that MsMYB is probably not regulated by miR858, like other members of subgroup 7.</abstract><cop>England</cop><pub>John Wiley & Sons, Inc</pub><pmid>28160379</pmid><doi>10.1111/pbi.12701</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0003-0553-272X</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant biotechnology journal, 2017-09, Vol.15 (9), p.1105-1119 |
| issn | 1467-7644 1467-7652 |
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| source | MEDLINE; Wiley Online Library Open Access; DOAJ Directory of Open Access Journals; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Amino Acid Sequence Base Sequence Biosynthesis Comparative analysis Design engineering Diphosphates - metabolism Diterpenes - metabolism Ectopic expression essential oil crops Essential oils Gene Expression Gene Expression Regulation, Plant gene overexpression genes Genetic engineering genetically modified organisms Geranyltranstransferase - genetics Geranyltranstransferase - metabolism GPPS Mentha spicata Mentha spicata - cytology Mentha spicata - genetics Mentha spicata - metabolism Metabolites Monoterpenes Monoterpenes - metabolism monoterpenoids MYB gene Nicotiana - cytology Nicotiana - genetics Nicotiana - metabolism Ocimum basilicum Ocimum basilicum - cytology Ocimum basilicum - genetics Ocimum basilicum - metabolism Oils, Volatile - metabolism Phylogeny Physiological aspects Plant Leaves - genetics Plant Leaves - metabolism Plant metabolites Plant Oils - metabolism Plant Proteins - genetics Plant Proteins - metabolism Plants Plants (botany) Plants, Genetically Modified R2R3‐MYB Regulatory mechanisms (biology) Ribonucleic acid RNA RNA-mediated interference Secondary Metabolism Secondary metabolites sequence analysis Sesquiterpenes - metabolism spearmint terpene Tobacco transcription factor transcription factors Transcription Factors - genetics Transcription Factors - metabolism Trichomes |
| title | Spearmint R2R3‐MYB transcription factor MsMYB negatively regulates monoterpene production and suppresses the expression of geranyl diphosphate synthase large subunit (MsGPPS.LSU) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T11%3A49%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Spearmint%20R2R3%E2%80%90MYB%20transcription%20factor%20MsMYB%20negatively%20regulates%20monoterpene%20production%20and%20suppresses%20the%20expression%20of%20geranyl%20diphosphate%20synthase%20large%20subunit%20(MsGPPS.LSU)&rft.jtitle=Plant%20biotechnology%20journal&rft.au=Reddy,%20Vaishnavi%20Amarr&rft.date=2017-09&rft.volume=15&rft.issue=9&rft.spage=1105&rft.epage=1119&rft.pages=1105-1119&rft.issn=1467-7644&rft.eissn=1467-7652&rft_id=info:doi/10.1111/pbi.12701&rft_dat=%3Cgale_pubme%3EA733243661%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1927660038&rft_id=info:pmid/28160379&rft_galeid=A733243661&rfr_iscdi=true |